Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 
or upload a file of gene symbols


Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

NCBP2 Gene

protein-coding   GIFtS: 61
GCID: GC03M196662

Nuclear Cap Binding Protein Subunit 2, 20kDa

(Previous names: nuclear cap binding protein subunit 2, 20kD)
Microbiology & Infectious Diseases Congress
  Search for NCBP2
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
Nuclear Cap Binding Protein Subunit 2, 20kDa1 2     NCBP-Interacting Protein 12 3
Cell Proliferation-Inducing Gene 55 Protein2 3     Nuclear Cap Binding Protein Subunit 2, 20kD1
20 KDa Nuclear Cap-Binding Protein2 3     CBC22
CBP202 3     NCBP Interacting Protein 12
NIP12 3     Nuclear Cap-Binding Protein Subunit 22
NCBP 20 KDa Subunit2 3     

External Ids:    HGNC: 76591   Entrez Gene: 229162   Ensembl: ENSG000001145037   OMIM: 6051335   UniProtKB: P522983   

Export aliases for NCBP2 gene to outside databases

Previous GC identifers: GC03M192627 GC03M197011 GC03M198069 GC03M197988 GC03M198150 GC03M193962


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for NCBP2 Gene:
The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the
monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly
found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end
processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different
isoforms have been found for this gene. (provided by RefSeq, Jul 2008)

GeneCards Summary for NCBP2 Gene: 
NCBP2 (nuclear cap binding protein subunit 2, 20kDa) is a protein-coding gene. Among its related super-pathways are mRNA Processing and Formation of HIV-1 elongation complex containing HIV-1 Tat. GO annotations related to this gene include RNA binding and RNA cap binding. An important paralog of this gene is NCBP2L.

UniProtKB/Swiss-Prot: NCBP2_HUMAN, P52298
Function: Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs
and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA
decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in
mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA
export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The
CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex
is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is
replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC
complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the
CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one
exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The
CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not
participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in
microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA
interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA
deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires
NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding
state with the cap structure




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000003.11  NT_029928.13  NC_018914.2  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the NCBP2 gene promoter:
         NF-1/L   NF-1   MyoD   HNF-4alpha2   CUTL1   HNF-4alpha1   FOXL1   c-Ets-1   AREB6   PPAR-alpha   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidNCBP2 promoter sequence
   Search SABiosciences Chromatin IP Primers for NCBP2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NCBP2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3q29   Ensembl cytogenetic band:  3q29   HGNC cytogenetic band: 3q29

NCBP2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NCBP2 gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03M196662:  view genomic region     (about GC identifiers)

Start:
196,662,273 bp from pter      End:
196,669,468 bp from pter
Size:
7,196 bases      Orientation:
minus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: NCBP2_HUMAN, P52298 (See protein sequence)
Recommended Name: Nuclear cap-binding protein subunit 2  
Size: 156 amino acids; 18001 Da
Subunit: Component of the nuclear cap-binding complex (CBC), a heterodimer composed of NCBP1/CBP80 and NCBP2/CBP20
that interacts with m7GpppG-capped RNA. Found in a U snRNA export complex with RNUXA/PHAX, NCBP1/CBP80,
NCBP2/CBP20, RAN, XPO1 and m7G-capped RNA. Interaction with RNUXA/PHAX. Is part of the exon junction complex
(EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, UPF3B, UPF2, ALYREF/THOC4 and/or REFBP2. Interacts with
EIF4G1, HNRNPF, HNRNPH1 and ALYREF/THOC4/ALY
Subcellular location: Nucleus. Cytoplasm
6/8 PDB 3D structures from and Proteopedia for NCBP2 (see all 8):
1H2T (3D)        1H2U (3D)        1H2V (3D)        1H6K (3D)        1N52 (3D)        1N54 (3D)    
Secondary accessions: B2RE91 B4DMK7 Q14924 Q2TS50
Alternative splicing: 2 isoforms:  P52298-1   P52298-2   

Explore the universe of human proteins at neXtProt for NCBP2: NX_P52298

Explore proteomics data for NCBP2 at MOPED 

Post-translational modifications:

  • View modification sites using PhosphoSitePlus
  • View neXtProt modification sites for NX_P52298

  • NCBP2 Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

    NCBP2 Protein Expression
    REFSEQ proteins (2 alternative transcripts): 
    NP_001036005.1  NP_031388.2  

    ENSEMBL proteins: 
     ENSP00000413518   ENSP00000326806   ENSP00000414122   ENSP00000397619   ENSP00000412785  
     ENSP00000394105   ENSP00000389315   ENSP00000402549  
    Reactome Protein details: P52298
    Human Recombinant Protein Products for NCBP2: 
    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein for NCBP2
    OriGene Protein Over-expression Lysate for NCBP2
    OriGene MassSpec for NCBP2 
    OriGene Custom Protein Services for NCBP2
    GenScript Custom Purified and Recombinant Proteins Services for NCBP2
    Novus Biologicals NCBP2 Proteins
    Novus Biologicals NCBP2 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates 
    ProSpec Recombinant Protein for NCBP2
    Cloud-Clone Corp. Proteins for NCBP2 

    Gene Ontology (GO): 5/7 cellular component terms (GO ID links to tree view) (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus NAS7651522
    GO:0005654nucleoplasm TAS--
    GO:0005730NOT nucleolus IDA--
    GO:0005737cytoplasm IDA8601613
    GO:0005829cytosol TAS--

    NCBP2 for ontologies           About GeneDecksing



    NCBP2 Antibody Products: 
    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    Browse OriGene Antibodies
    OriGene Custom Antibody Services for NCBP2
    GenScript Custom Superior Antibodies Services for NCBP2
    Novus Biologicals NCBP2 Antibodies
    Abcam antibodies for NCBP2
    Cloud-Clone Corp. Antibodies for NCBP2 
    ThermoFisher Antibodies for NCBP2
    LSBio Antibodies in human, mouse, rat for NCBP2 

    Assay Products for NCBP2: 
    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for NCBP2
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for NCBP2
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for NCBP2 
    Cloud-Clone Corp. CLIAs for NCBP2


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    HGNC Gene Families: 
    RBM: RNA binding motif (RRM) containing

    3 InterPro protein domains:
     IPR012677 Nucleotide-bd_a/b_plait
     IPR027157 NCBP2
     IPR000504 RRM_dom

    Graphical View of Domain Structure for InterPro Entry P52298

    ProtoNet protein and cluster: P52298

    1 Blocks protein domain: IPB000504 RNA-binding region RNP-1 (RNA recognition motif)

    UniProtKB/Swiss-Prot: NCBP2_HUMAN, P52298
    Similarity: Belongs to the RRM NCBP2 family
    Similarity: Contains 1 RRM (RNA recognition motif) domain


    NCBP2 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: NCBP2_HUMAN, P52298
    Function: Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs
    and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA
    decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in
    mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA
    export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The
    CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex
    is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is
    replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC
    complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the
    CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one
    exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The
    CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not
    participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in
    microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA
    interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA
    deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires
    NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding
    state with the cap structure

         Genatlas biochemistry entry for NCBP2:
    nuclear cap binding protein subunit 2,component of thecap-binding protein complex in association with NCBP2 and
    other proteins,mediating premRNA splicing and export of U small nuclear RNAs to the cytoplasm

         Gene Ontology (GO): 5/6 molecular function terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166nucleotide binding IEA--
    GO:0000339RNA cap binding NAS7651522
    GO:0000340RNA 7-methylguanosine cap binding IDA12434151
    GO:0003676nucleic acid binding ----
    GO:0003723NOT RNA binding IDA7478990
         
    NCBP2 for ontologies           About GeneDecksing


    Phenotypes:
         2 GenomeRNAi human phenotypes for NCBP2:
     Increased G1 DNA content  Increased gamma-H2AX phosphory 

    Animal Models:
       inGenious Targeting Laboratory - Custom generated mouse model solutions for NCBP2 
       inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for NCBP2

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for NCBP2 
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for NCBP2 

    miRNA
    Products:
        
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat NCBP2
    8/11 QIAGEN miScript miRNA Assays for microRNAs that regulate NCBP2 (see all 11):
    hsa-miR-3163 hsa-miR-3910 hsa-miR-1283 hsa-miR-631 hsa-miR-1267 hsa-miR-507 hsa-miR-3661 hsa-miR-4267
    SwitchGear 3'UTR luciferase reporter plasmidNCBP2 3' UTR sequence
    Inhib. RNA
    Products:
        
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for NCBP2
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NCBP2

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for NCBP2
    Sirion Biotech Customized adenovirus for overexpression of NCBP2

    Clone
    Products:
         
    Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene clones in human, mouse for NCBP2 (see all 11)
    OriGene ORF clones in mouse, rat for NCBP2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): NCBP2 (NM_001042540)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NCBP2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NCBP2
    Sirion Biotech Customized lentivirus for stable overexpression of NCBP2 
                         Customized lentivirus expression plasmids for stable overexpression of NCBP2 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for NCBP2
    Search LifeMap BioReagents cell lines for NCBP2
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NCBP2


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
    About This Section

    SuperPaths for NCBP2 About   (see all 17)                                                                                              See pathways by source

    SuperPathSelected contained pathways About (see all per SuperPath)
    1mRNA Splicing - Major Pathway
    Processing of Capped Intron-Containing Pre-mRNA0.85
    mRNA processing0.46
    mRNA Processing0.85
    mRNA Splicing - Minor Pathway0.40
    mRNA Splicing - Major Pathway0.82
    Spliceosome0.31
    mRNA Splicing0.82
    2Formation of RNA Pol II elongation complex
    mRNA Capping0.93
    Formation of HIV elongation complex in the absence of HIV Tat0.76
    Formation of HIV-1 elongation complex containing HIV-1 Tat0.78
    RNA Polymerase II Transcription Elongation0.76
    Tat-mediated elongation of the HIV-1 transcript0.78
    Formation of RNA Pol II elongation complex 0.76
    HIV Transcription Elongation0.78
    RNA Polymerase II Pre-transcription Events0.72
    3RNA Polymerase II Transcription Termination
    Post-Elongation Processing of Intron-Containing pre-mRNA0.79
    RNA Polymerase II Transcription Termination0.79
    Cleavage of Growing Transcript in the Termination Region 0.79
    Post-Elongation Processing of the Transcript0.79
    mRNA 3'-end processing0.79
    4Viral mRNA Translation
    Nonsense Mediated Decay Independent of the Exon Junction Complex0.94
    Nonsense Mediated Decay Enhanced by the Exon Junction Complex0.84
    Nonsense-Mediated Decay0.84
    5HIV Life Cycle
    HIV Life Cycle0.92
    HIV Infection0.61
    Late Phase of HIV Life Cycle0.92

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 BioSystems Pathway for NCBP2
        mRNA processing

    5/47        Reactome Pathways for NCBP2 (see all 47)
        Transport of Mature mRNA derived from an Intron-Containing Transcript
    Tat-mediated elongation of the HIV-1 transcript
    Transport of Mature Transcript to Cytoplasm
    mRNA Splicing
    Cleavage of Growing Transcript in the Termination Region


    3         Kegg Pathways  (Kegg details for NCBP2):
        RNA transport
    mRNA surveillance pathway
    Spliceosome


    NCBP2 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for NCBP2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/338 Interacting proteins for NCBP2 (P522981, 2, 3 ENSP000003268064) via UniProtKB, MINT, STRING, and/or I2D (see all 338)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    NCBP1Q091611, 2, 3, ENSP000003642894EBI-464729,EBI-464743 MINT-24994 MINT-8335957 I2D: score=7 STRING: ENSP00000364289
    SF3A2Q154282, 3, ENSP000002214944MINT-5205908 I2D: score=1 STRING: ENSP00000221494
    ENSG00000206353Q154773I2D: score=1 
    ENSG00000223493Q154773I2D: score=1 
    ENSG00000225737Q154773I2D: score=1 
    About this table

    Gene Ontology (GO): 5/22 biological process terms (GO ID links to tree view) (see all 22):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000184nuclear-transcribed mRNA catabolic process, nonsense-mediated decay TAS--
    GO:0000387spliceosomal snRNP assembly TAS--
    GO:0000398mRNA splicing, via spliceosome TAS--
    GO:0006366transcription from RNA polymerase II promoter TAS--
    GO:0006368transcription elongation from RNA polymerase II promoter TAS--

    NCBP2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for NCBP2

    Search CenterWatch for drugs/clinical trials and news about NCBP2

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for NCBP2 gene (2 alternative transcripts): 
    NM_001042540.1  NM_007362.3  

    Unigene Cluster for NCBP2:

    Nuclear cap binding protein subunit 2, 20kDa
    Hs.591671  [show with all ESTs]
    Unigene Representative Sequence: AK093216
    13 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000467803 ENST00000447325(uc003fxb.1 uc011btz.1) ENST00000463783(uc003fxf.3)
    ENST00000321256(uc003fxd.1 uc003fxe.1) ENST00000428425(uc003fxc.1)
    ENST00000427641 ENST00000452404 ENST00000468923 ENST00000422610 ENST00000411704
    ENST00000479647 ENST00000482976 ENST00000455953
    Congresses - knowledge worth sharing:  
    European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) 10 - 13 May 2014

    miRNA
    Products:
         
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat NCBP2
    8/11 QIAGEN miScript miRNA Assays for microRNAs that regulate NCBP2 (see all 11):
    hsa-miR-3163 hsa-miR-3910 hsa-miR-1283 hsa-miR-631 hsa-miR-1267 hsa-miR-507 hsa-miR-3661 hsa-miR-4267
    SwitchGear 3'UTR luciferase reporter plasmidNCBP2 3' UTR sequence
    Inhib. RNA
    Products:
         
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for NCBP2
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NCBP2
    Clone
    Products:
         
    OriGene clones in human, mouse for NCBP2 (see all 11)
    OriGene ORF clones in mouse, rat for NCBP2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): NCBP2 (NM_001042540)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NCBP2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NCBP2
    Sirion Biotech Customized lentivirus for stable overexpression of NCBP2 
                         Customized lentivirus expression plasmids for stable overexpression of NCBP2 
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for NCBP2
    OriGene qSTAR qPCR primer pairs in human, mouse for NCBP2
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat NCBP2
      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NCBP2
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NCBP2

    Additional mRNA sequence: 

    AK093216.1 AK296144.1 AK297506.1 AK308192.1 AK315903.1 AK316601.1 AL110262.1 AY644767.1 
    BC001255.1 BT006842.1 D59253.1 X84157.1 

    24/26 DOTS entries (see all 26):

    DT.95313311  DT.97859620  DT.99942089  DT.100877814  DT.420024  DT.100877810  DT.92354625  DT.100032275 
    DT.120865800  DT.120865813  DT.100685365  DT.100877808  DT.120865820  DT.91771720  DT.120865837  DT.100877807 
    DT.102842776  DT.40205728  DT.100037245  DT.120865798  DT.120865844  DT.120865861  DT.121629946  DT.100877818 

    24/396 AceView cDNA sequences (see all 396):

    BU195134 BC001255 BG025812 BP376641 BP375943 BQ962214 BQ051165 CB216069 
    AL705476 X84157 BG678722 CB152214 BM459785 BU781754 BF793977 BP335681 
    BM770757 BP347677 BP337230 BP342620 NM_007362 AW405730 BU184430 BM836154 

    GeneLoc Exon Structure

    5/11 Alternative Splicing Database (ASD) splice patterns (SP) for NCBP2 (see all 11)    About this scheme

    ExUns: 1a · 1b · 1c · 1d · 1e ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b · 6c · 6d ^ 7a · 7b · 7c · 7d · 7e ^ 8a · 8b · 8c · 8d · 8e · 8f ·
    SP1:                                      -     -     -     -                             -                       -     -                                       
    SP2:                                -     -     -     -     -     -                       -                       -     -                                       
    SP3:                                      -     -     -     -     -                       -                       -     -                                       
    SP4:                                -     -     -     -     -     -           -     -     -     -                 -     -                                       
    SP5:                                            -     -     -     -                       -                       -     -                                       

    ExUns: 8g · 8h
    SP1:            
    SP2:            
    SP3:            
    SP4:            
    SP5:            


    ECgene alternative splicing isoforms for NCBP2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    NCBP2 expression in normal human tissues (normalized intensities)      NCBP2 embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AGGACCAGCA
    NCBP2 Expression
    About this image


    NCBP2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database 
     5/33 selected tissues (see all 33) fully expand
     
     Epithelium
             vagina ; squamous epithelial cells   
     
     Uterus
             uterus, post-menopause ; glandular cells   
     
     Testis (Reproductive System)    fully expand to see all 4 entries
             Leydig Cells Testis Interstitium
             seminal vesicle ; glandular cells   
     
     Colon (Gastrointestinal Tract)    fully expand to see all 4 entries
             colon ; peripheral nerve/ganglion   
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex

    See NCBP2 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for NCBP2

    SOURCE GeneReport for Unigene cluster: Hs.591671
        SABiosciences Custom PCR Arrays for NCBP2
    Primer
    Products:
    OriGene qPCR primer pairs and template standards for NCBP2
    OriGene qSTAR qPCR primer pairs in human, mouse for NCBP2
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat NCBP2
    QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NCBP2
    QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NCBP2
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NCBP2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for NCBP2 gene from 10/21 species (see all 21)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Ncbp21 , 5 nuclear cap binding protein subunit 21, 5 90.81(n)1
    98.08(a)1
      16 (22.40 cM)5
    680921  NM_026554.41  NP_080830.11 
     319485135 
    chicken
    (Gallus gallus)
    Aves NCBP21 nuclear cap binding protein subunit 2, 20kDa 75.48(n)
    88.39(a)
      424889  XM_422700.3  XP_422700.3 
    lizard
    (Anolis carolinensis)
    Reptilia --
    Uncharacterized protein
    68(a)
    possible ortholog
    GL343758.1(73652-77886)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.16442 X.laevis mRNA for CAP binding protein 20kDa 81.29(n)    X84788.1 
    zebrafish
    (Danio rerio)
    Actinopterygii ncbp22 nuclear cap binding protein subunit 2 73.2(n)   192325  AY099530.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Cbp203 mRNA splicing RNA binding 78(a)   90E7   --
    worm
    (Caenorhabditis elegans)
    Secernentea F26A3.23 Cap binding protein 65(a)   I(7650521-7651401)   --
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes CBC2(YPL178W)4 Small subunit of the heterodimeric cap binding complex more   --   16(212158-212784) 855925  NP_015147.1 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons CBP201 nuclear cap-binding protein subunit 2 61.7(n)
    68.09(a)
      834443  NM_001085236.1  NP_001078705.1 
    rice
    (Oryza sativa)
    Liliopsida AK061262.12   -- 72.99(n)    AK061262.1 


    ENSEMBL Gene Tree for NCBP2 (if available)
    TreeFam Gene Tree for NCBP2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for NCBP2 gene
    NCBP2L2  
    3 SIMAP similar genes for NCBP2 using alignment to 6 protein entries:     NCBP2_HUMAN (see all proteins):
    DKFZp686G18222    NCBP2L    CIRBP

    NCBP2 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/174 SNPs in NCBP2 are shown (see all 174)    About this table     
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 3 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1444898171,2
    --196662103(+) TCTGTA/GCGCTC 2 -- ds50010--------
    rs68066181,2
    C,F,A,H--196662154(+) TCAAAA/GTGGAC 2 -- ds500118Minor allele frequency- G:0.11NA EA NS WA 1810
    rs1446294481,2
    --196662162(+) GACACA/GCGAAT 2 -- ds50010--------
    rs1140765441,2
    F--196662163(+) ACACGC/TGAATT 2 -- ds50011Minor allele frequency- T:0.05WA 118
    rs1151347531,2
    C,F--196662164(+) CACGCG/AAATTG 2 -- ds50011Minor allele frequency- A:0.05WA 118
    rs1884664971,2
    --196662214(+) TGTATC/GCAGAG 2 -- ds50010--------
    rs769020261,2
    F--196662265(+) AACAGG/ACAGCA 2 -- ds50011Minor allele frequency- A:0.01EA 120
    rs67969631,2
    C,F,H--196662275(+) AACAAC/TGATAA 2 -- ut3120Minor allele frequency- T:0.22EA NS NA WA CSA 2122
    rs773873491,2
    F--196662296(+) CTTTAC/TTGAAA 2 -- ut310--------
    rs1922552981,2
    --196662299(+) TATTGA/GAACCT 2 -- ut310--------

    HapMap Linkage Disequilibrium report for NCBP2 (196662273 - 196669468 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 1 variation for NCBP2:    About this table     
    Variant IDTypeSubtypePubMed ID
    nsv819558CNV Loss19587683

    SABiosciences Cancer Mutation PCR Assays
    SeqTarget long-range PCR primers for resequencing NCBP2
    DNA2.0 Custom Variant and Variant Library Synthesis for NCBP2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    NCBP2 for disorders           About MalaCards
    OMIM gene information: 605133    OMIM disorders: --


    NCBP2 for disorders           About GeneDecksing

    Human Genome Epidemiology (HuGE) Navigator: NCBP2 (1 document)

    Export disorders for NCBP2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for NCBP2 gene, integrated from 9 sources (see all 60):
    (articles sorted by number of sources associating them with NCBP2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. A cap-binding protein complex mediating U snRNA export. (PubMed id 7651522)1, 2, 3, 9 Izaurralde E....Mattaj A.W. (1995)
    2. Identification of the factors that interact with NCBP, an 80 kDa nuclear cap binding protein. (PubMed id 7478990)1, 2, 3 Kataoka N.... Shimura Y. (1995)
    3. A nuclear cap-binding complex binds Balbiani ring pre-mRNA cotranscriptionally and accompanies the ribonucleoprotein particle during nuclear export. (PubMed id 8601613)1, 2, 9 Visa N.... Mattaj I.W. (1996)
    4. Evidence for a pioneer round of mRNA translation: mRNAs subject to nonsense-mediated decay in mammalian cells are bound by CBP80 and CBP20. (PubMed id 11551508)1, 2, 9 Ishigaki Y.... Maquat L.E. (2001)
    5. Interaction between the human nuclear cap-binding protein complex and hnRNP F. (PubMed id 9111328)1, 2, 9 Gamberi C.... Mattaj I.W. (1997)
    6. NMD resulting from encephalomyocarditis virus IRES-directed translation initiation seems to be restricted to CBP80/20-bound mRNA. (PubMed id 18369367)1, 2 Woeller C.F.... Maquat L.E. (2008)
    7. Failsafe nonsense-mediated mRNA decay does not detectably target eIF4E-bound mRNA. (PubMed id 17873884)1, 2 Matsuda D.... Maquat L.E. (2007)
    8. Human mRNA export machinery recruited to the 5' end of mRNA. (PubMed id 17190602)1, 2 Cheng H.... Reed R. (2006)
    9. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    10. eIF4G is required for the pioneer round of translation in mammalian cells. (PubMed id 15361857)1, 2 Lejeune F.... Maquat L.E. (2004)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 22916 HGNC: 7659 AceView: NCBP2 Ensembl:ENSG00000114503 euGenes: HUgn22916
    ECgene: NCBP2 Kegg: 22916 H-InvDB: NCBP2

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for NCBP2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for NCBP2 gene:
    Search GeneIP for patents involving NCBP2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Sirion Biotech, Cell lines from GenScript, and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

     
     EMD Millipore Custom Antibody & Bulk Services
     EMD Millipore Preclinical / Clinical Development Services
     EMD Millipore Immunoassay Services
     EMD Millipore Target Screening & Profiling Services

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Kinase Activity Assays/Reagents  
     Browse ELISpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Multiplex/Array Assay Kits/Reagents  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Protease Activity Assays and Reagents   Browse Recombinant/Natural Proteins  
     Browse Stem Cell Products   Browse Tocris Biochemicals & Compounds  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     Browse OriGene Antibodies   OriGene RNAi products in human, mouse, rat for NCBP2  
     OriGene qPCR primer pairs and template standards for NCBP2   OriGene Protein Over-expression Lysate for NCBP2  
     OriGene MassSpec something-or-other for NCBP2   OriGene clones in human, mouse for NCBP2  
     OriGene qSTAR qPCR primer pairs in human, mouse for NCBP2   OriGene Purified Protein for NCBP2  
     OriGene ORF clones in mouse, rat for NCBP2   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for NCBP2   OriGene Custom Protein Services for NCBP2  

     
     
     QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat NCBP2
     QIAGEN SeqTarget long-range PCR primers for resequencing NCBP2
     QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NCBP2
     QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NCBP2
     QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NCBP2
     QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NCBP2
     GenScript Custom Purified and Recombinant Proteins Services for NCBP2 GenScript cDNA clones with any tag delivered in your preferred vector for NCBP2
     GenScript Custom Assay Services for NCBP2 GenScript Custom Superior Antibodies Services for NCBP2
     GenScript Custom overexpressing Cell Line Services for NCBP2 CloneReady with Over 120,000 Genes
     Gene Synthesis: Any Gene in Any Vector Vector-based siRNA and miRNA, Ready for Transfection
     Gene Mutant Library, Variants up to 10^11 Plasmid Preparation
     Custom Peptide Services
     Search for Antibodies & Assays

     Regulatory tfbs in NCBP2 promoter
     Search Chromatin IP Primers for NCBP2
     RT2 qPCR Primer Assay in human, mouse, rat NCBP2
     GNC Network for NCBP2
     SABiosciences Custom PCR Arrays for NCBP2
     Search Tocris compounds for NCBP2
     Browse Sino Biological Proteins and Antibodies
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies
     Novus Tissue Slides
     NCBP2 antibodies
     NCBP2 proteins
     NCBP2 lysates
     Antibodies for NCBP2
     See all of Abcam's Antibodies, Kits and Proteins for NCBP2
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Recombinant Protein for NCBP2




     Proteins for NCBP2
     Antibodies for NCBP2
     ELISAs for NCBP2
     CLIAs for NCBP2
     Search LifeMap BioReagents cell lines for NCBP2
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NCBP2
     SwitchGear 3'UTR luciferase reporter plasmids for NCBP2
     SwitchGear Promoter luciferase reporter plasmids for NCBP2
     ThermoFisher Antibodies for NCBP2
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NCBP2
     inGenious Targeting Laboratory - Custom generated mouse model solutions for NCBP2
     inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for NCBP2
    Customized:
     lentivirus for stable overexpression of NCBP2
     lentivirus expression plasmids for stable overexpression of NCBP2
     adenovirus for overexpression of NCBP2
     LSBio Antibodies in human, mouse, rat for NCBP2
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
           
    GeneCards Homepage - Last full update: 23 Oct 2013 - Incrementals: 4 Nov 2013 , 7 Nov 2013 , 23 Jan 2014

    View Random Gene

    Category
    (GIFtS: 73)
    transforming growth factor, beta 1
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      NCBP2 gene at Home site.
    hostname: 356977-web1.xennexinc.com index build: 106 solr: 1.4