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NCBP2 Gene

protein-coding   GIFtS: 63
GCID: GC03M196662

Nuclear Cap Binding Protein Subunit 2, 20kDa

(Previous names: nuclear cap binding protein subunit 2, 20kD)
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Nuclear Cap Binding Protein Subunit 2, 20kDa1 2     NCBP-Interacting Protein 12 3
Cell Proliferation-Inducing Gene 55 Protein2 3     Nuclear Cap Binding Protein Subunit 2, 20kD1
20 KDa Nuclear Cap-Binding Protein2 3     CBC22
CBP202 3     NCBP Interacting Protein 12
NIP12 3     Nuclear Cap-Binding Protein Subunit 22
NCBP 20 KDa Subunit2 3     

External Ids:    HGNC: 76591   Entrez Gene: 229162   Ensembl: ENSG000001145037   OMIM: 6051335   UniProtKB: P522983   

Export aliases for NCBP2 gene to outside databases

Previous GC identifers: GC03M192627 GC03M197011 GC03M198069 GC03M197988 GC03M198150 GC03M193962


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for NCBP2 Gene:
The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the
monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly
found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end
processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different
isoforms have been found for this gene. (provided by RefSeq, Jul 2008)

GeneCards Summary for NCBP2 Gene:
NCBP2 (nuclear cap binding protein subunit 2, 20kDa) is a protein-coding gene. GO annotations related to this gene include RNA binding and RNA cap binding. An important paralog of this gene is NCBP2L.

UniProtKB/Swiss-Prot: NCBP2_HUMAN, P52298
Function: Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs
and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA
decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in
mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA
export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The
CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex
is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is
replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC
complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the
CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one
exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The
CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not
participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in
microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA
interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA
deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires
NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding
state with the cap structure




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000003.11  NT_005612.17  NC_018914.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the NCBP2 gene promoter:
         NF-1/L   NF-1   MyoD   HNF-4alpha2   CUTL1   HNF-4alpha1   FOXL1   c-Ets-1   AREB6   PPAR-alpha   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidNCBP2 promoter sequence
   Search Chromatin IP Primers for NCBP2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat NCBP2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3q29   Ensembl cytogenetic band:  3q29   HGNC cytogenetic band: 3q29

NCBP2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NCBP2 gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03M196662:  view genomic region     (about GC identifiers)

Start:
196,662,273 bp from pter      End:
196,669,468 bp from pter
Size:
7,196 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: NCBP2_HUMAN, P52298 (See protein sequence)
Recommended Name: Nuclear cap-binding protein subunit 2  
Size: 156 amino acids; 18001 Da
Subunit: Component of the nuclear cap-binding complex (CBC), a heterodimer composed of NCBP1/CBP80 and NCBP2/CBP20
that interacts with m7GpppG-capped RNA. Found in a U snRNA export complex with RNUXA/PHAX, NCBP1/CBP80,
NCBP2/CBP20, RAN, XPO1 and m7G-capped RNA. Interacts with RNUXA/PHAX, EIF4G1, HNRNPF, HNRNPH1 and
ALYREF/THOC4/ALY
Selected PDB 3D structures from and Proteopedia for NCBP2 (see all 8):
1H2T (3D)        1H2U (3D)        1H2V (3D)        1H6K (3D)        1N52 (3D)        1N54 (3D)    
Secondary accessions: B2RE91 B4DMK7 E9PAR5 Q14924 Q2TS50
Alternative splicing: 3 isoforms:  P52298-1   P52298-2   P52298-3   (Gene prediction based on EST data)

Explore the universe of human proteins at neXtProt for NCBP2: NX_P52298

Explore proteomics data for NCBP2 at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys120, Lys151
  • Modification sites at PhosphoSitePlus

  • See NCBP2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001036005.1  NP_031388.2  

    ENSEMBL proteins: 
     ENSP00000413518   ENSP00000326806   ENSP00000414122   ENSP00000397619   ENSP00000412785  
     ENSP00000394105   ENSP00000389315   ENSP00000402549  
    Reactome Protein details: P52298

    NCBP2 Human Recombinant Protein Products:

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    Novus Biologicals NCBP2 Proteins
    Novus Biologicals NCBP2 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    ProSpec Recombinant Protein for NCBP2
    Cloud-Clone Corp. Proteins for NCBP2

     
    Search eBioscience for Proteins for NCBP2 

    NCBP2 Antibody Products:

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    LSBio Antibodies in human, mouse, rat for NCBP2

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    Search eBioscience for ELISAs for NCBP2 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    RBM: RNA binding motif (RRM) containing

    3 InterPro protein domains:
     IPR012677 Nucleotide-bd_a/b_plait
     IPR027157 NCBP2
     IPR000504 RRM_dom

    Graphical View of Domain Structure for InterPro Entry P52298

    ProtoNet protein and cluster: P52298

    1 Blocks protein domain: IPB000504 RNA-binding region RNP-1 (RNA recognition motif)

    UniProtKB/Swiss-Prot: NCBP2_HUMAN, P52298
    Similarity: Belongs to the RRM NCBP2 family
    Similarity: Contains 1 RRM (RNA recognition motif) domain


    Find genes that share domains with NCBP2           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: NCBP2_HUMAN, P52298
    Function: Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs
    and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA
    decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in
    mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA
    export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The
    CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex
    is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is
    replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC
    complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the
    CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one
    exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The
    CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not
    participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in
    microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA
    interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA
    deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires
    NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding
    state with the cap structure

         Genatlas biochemistry entry for NCBP2:
    nuclear cap binding protein subunit 2,component of thecap-binding protein complex in association with NCBP2 and
    other proteins,mediating premRNA splicing and export of U small nuclear RNAs to the cytoplasm

         Gene Ontology (GO): Selected molecular function terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166nucleotide binding IEA--
    GO:0000339RNA cap binding NAS7651522
    GO:0000340RNA 7-methylguanosine cap binding IDA12434151
    GO:0003676nucleic acid binding ----
    GO:0003723NOT RNA binding IDA7478990
         
    Find genes that share ontologies with NCBP2           About GenesLikeMe


    Phenotypes:
         2 GenomeRNAi human phenotypes for NCBP2:
     Increased G1 DNA content  Increased gamma-H2AX phosphory 

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for NCBP2
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       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for NCBP2
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for NCBP2

    miRNA
    Products:
        
    miRTarBase miRNAs that target NCBP2:
    hsa-mir-19b-3p (MIRT050630), hsa-mir-10a-3p (MIRT038675), hsa-mir-192-5p (MIRT026313), hsa-mir-34a-5p (MIRT025377), hsa-mir-24-3p (MIRT030403)

    Block miRNA regulation of human, mouse, rat NCBP2 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate NCBP2 (see all 11):
    hsa-miR-3163 hsa-miR-3910 hsa-miR-1283 hsa-miR-631 hsa-miR-1267 hsa-miR-507 hsa-miR-3661 hsa-miR-4267
    SwitchGear 3'UTR luciferase reporter plasmidNCBP2 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat NCBP2

    Gene Editing
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    Clone
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NCBP2


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    NCBP2_HUMAN, P52298: Nucleus. Cytoplasm
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol4
    extracellular1

    Gene Ontology (GO): Selected cellular component terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus NAS7651522
    GO:0005654nucleoplasm TAS--
    GO:0005730NOT nucleolus IDA--
    GO:0005737cytoplasm IDA8601613
    GO:0005829cytosol TAS--

    Find genes that share ontologies with NCBP2           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for NCBP2 About   (see all 12)  
    See pathways by source

    SuperPathSelected contained pathways About (see all per SuperPath)
    1mRNA Splicing - Major Pathway
    mRNA Splicing - Major Pathway0.78
    mRNA processing0.44
    Processing of Capped Intron-Containing Pre-mRNA0.78
    mRNA Splicing - Minor Pathway0.38
    mRNA Splicing0.78
    2Formation of RNA Pol II elongation complex
    mRNA Capping0.93
    Formation of RNA Pol II elongation complex0.75
    Formation of the Early Elongation Complex0.77
    Formation of HIV elongation complex in the absence of HIV Tat0.75
    Formation of the HIV-1 Early Elongation Complex0.77
    RNA Polymerase II Pre-transcription Events0.73
    Formation of HIV-1 elongation complex containing HIV-1 Tat0.77
    Transcription of the HIV genome0.71
    3RNA Polymerase II Transcription Termination
    RNA Polymerase II Transcription Termination0.80
    SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs0.00
    mRNA 3-end processing0.80
    SLBP independent Processing of Histone Pre-mRNAs0.00
    Cleavage of Growing Transcript in the Termination Region0.80
    Processing of Intronless Pre-mRNAs0.00
    Processing of Capped Intronless Pre-mRNA0.52
    4Regulation of Glucokinase by Glucokinase Regulatory Protein
    Transport of the SLBP Dependant Mature mRNA0.92
    Transport of Mature mRNA Derived from an Intronless Transcript0.91
    Transport of Mature mRNAs Derived from Intronless Transcripts0.92
    snRNP Assembly0.54
    Transport of the SLBP independent Mature mRNA0.91
    Metabolism of non-coding RNA0.54
    5Influenza Viral RNA Transcription and Replication
    Nonsense Mediated Decay Independent of the Exon Junction Complex0.94
    Nonsense-Mediated Decay0.89
    Nonsense Mediated Decay Enhanced by the Exon Junction Complex0.89


    Find genes that share SuperPaths with NCBP2           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 BioSystems Pathway for NCBP2
        mRNA processing

    Selected Reactome Pathways for NCBP2 (see all 23)
        Transport of Mature mRNA derived from an Intron-Containing Transcript
    Formation of RNA Pol II elongation complex
    Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
    Transport of Mature mRNA Derived from an Intronless Transcript
    snRNP Assembly


    3 Kegg Pathways  (Kegg details for NCBP2):
        RNA transport
    mRNA surveillance pathway
    Spliceosome

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for NCBP2
    Interactions:

        GeneGlobe Interaction Network for NCBP2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for NCBP2 (P522981, 2, 3 ENSP000003268064) via UniProtKB, MINT, STRING, and/or I2D (see all 479)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    NCBP1Q091611, 2, 3, ENSP000003642894EBI-464729,EBI-464743 MINT-24994 MINT-8335957 I2D: score=7 STRING: ENSP00000364289
    SF3A2Q154282, 3, ENSP000002214944MINT-5205908 I2D: score=1 STRING: ENSP00000221494
    ENSG00000206353Q154773I2D: score=1 
    ENSG00000223493Q154773I2D: score=1 
    ENSG00000225737Q154773I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 22):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000184nuclear-transcribed mRNA catabolic process, nonsense-mediated decay TAS--
    GO:0000387spliceosomal snRNP assembly TAS--
    GO:0000398mRNA splicing, via spliceosome TAS--
    GO:0006366transcription from RNA polymerase II promoter TAS--
    GO:0006368transcription elongation from RNA polymerase II promoter TAS--

    Find genes that share ontologies with NCBP2           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for NCBP2



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

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    REFSEQ mRNAs for NCBP2 gene (2 alternative transcripts): 
    NM_001042540.1  NM_007362.3  

    Unigene Cluster for NCBP2:

    Nuclear cap binding protein subunit 2, 20kDa
    Hs.591671  [show with all ESTs]
    Unigene Representative Sequence: AK093216
    13 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000467803 ENST00000447325(uc003fxb.1 uc011btz.1) ENST00000463783(uc003fxf.3)
    ENST00000321256(uc003fxd.1 uc003fxe.1) ENST00000428425(uc003fxc.1)
    ENST00000427641 ENST00000452404 ENST00000468923 ENST00000422610 ENST00000411704
    ENST00000479647 ENST00000482976 ENST00000455953
    miRNA
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    hsa-miR-3163 hsa-miR-3910 hsa-miR-1283 hsa-miR-631 hsa-miR-1267 hsa-miR-507 hsa-miR-3661 hsa-miR-4267
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    GenScript: all cDNA clones in your preferred vector (see all 2): NCBP2 (NM_001042540)
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NCBP2
    Primer
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    OriGene qSTAR qPCR primer pairs in human, mouse for NCBP2
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NCBP2
      QuantiTect SYBR Green Assays in human, mouse, rat NCBP2
      QuantiFast Probe-based Assays in human, mouse, rat NCBP2

    Additional mRNA sequence: 

    AK093216.1 AK296144.1 AK297506.1 AK308192.1 AK315903.1 AK316601.1 AL110262.1 AY644767.1 
    BC001255.1 BT006842.1 D59253.1 X84157.1 

    Selected DOTS entries (see all 26):

    DT.95313311  DT.97859620  DT.99942089  DT.100877814  DT.420024  DT.100877810  DT.92354625  DT.100032275 
    DT.120865800  DT.120865813  DT.100685365  DT.100877808  DT.120865820  DT.91771720  DT.120865837  DT.100877807 
    DT.102842776  DT.40205728  DT.100037245  DT.120865798  DT.120865844  DT.120865861  DT.121629946  DT.100877818 

    Selected AceView cDNA sequences (see all 396):

    BG678722 BP348879 BQ435070 BI089929 CB152083 BQ962214 CB152214 BU183827 
    BQ636686 BU781754 CB216069 BU579958 BQ270359 AI955092 BF793977 NM_007362 
    BU195134 BP375943 AW405730 BM802062 BG576653 CR590424 BI518581 AA436754 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for NCBP2 (see all 11)    About this scheme

    ExUns: 1a · 1b · 1c · 1d · 1e ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b · 6c · 6d ^ 7a · 7b · 7c · 7d · 7e ^ 8a · 8b · 8c · 8d · 8e · 8f ·
    SP1:                                      -     -     -     -                             -                       -     -                                       
    SP2:                                -     -     -     -     -     -                       -                       -     -                                       
    SP3:                                      -     -     -     -     -                       -                       -     -                                       
    SP4:                                -     -     -     -     -     -           -     -     -     -                 -     -                                       
    SP5:                                            -     -     -     -                       -                       -     -                                       

    ExUns: 8g · 8h
    SP1:            
    SP2:            
    SP3:            
    SP4:            
    SP5:            


    ECgene alternative splicing isoforms for NCBP2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    NCBP2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AGGACCAGCA
    NCBP2 Expression
    About this image


    NCBP2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 8) fully expand
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex
     
     Ovary (Reproductive System)    fully expand to see all 2 entries
             Ovarian Mesenchymal Stroma Cells Ovary Interstitium
             Oviduct
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Testis (Reproductive System)
             Leydig Cells Testis Interstitium
    NCBP2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    NCBP2 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.591671
        Custom PCR Arrays for NCBP2
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for NCBP2 gene from Selected species (see all 21)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Ncbp21 , 5 nuclear cap binding protein subunit 21, 5 90.81(n)1
    98.08(a)1
      16 (22.40 cM)5
    680921  NM_026554.41  NP_080830.11 
     319485135 
    chicken
    (Gallus gallus)
    Aves NCBP21 nuclear cap binding protein subunit 2, 20kDa 75.48(n)
    88.39(a)
      424889  NM_001277455.1  NP_001264384.1 
    lizard
    (Anolis carolinensis)
    Reptilia --
    Uncharacterized protein
    68(a)
    1 → many
    GL343758.1(73652-77886)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.16442 X.laevis mRNA for CAP binding protein 20kDa 81.29(n)    X84788.1 
    zebrafish
    (Danio rerio)
    Actinopterygii ncbp22 nuclear cap binding protein subunit 2 73.2(n)   192325  AY099530.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Cbp203 mRNA splicing RNA binding 78(a)   90E7   --
    worm
    (Caenorhabditis elegans)
    Secernentea F26A3.23 Cap binding protein 65(a)   I(7650521-7651401)   --
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes CBC2(YPL178W)4 Small subunit of the heterodimeric cap binding complex more   --   16(212158-212784) 855925  NP_015147.1 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons CBP201 CBP20 61.5(n)
    67.61(a)
      834443  NM_001085236.1  NP_001078705.1 
    rice
    (Oryza sativa)
    Liliopsida AK061262.12   -- 72.99(n)    AK061262.1 


    ENSEMBL Gene Tree for NCBP2 (if available)
    TreeFam Gene Tree for NCBP2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for NCBP2 gene
    NCBP2L2  
    3 SIMAP similar genes for NCBP2 using alignment to 5 protein entries:     NCBP2_HUMAN (see all proteins):
    DKFZp686G18222    NCBP2L    CIRBP

    Find genes that share paralogs with NCBP2           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for NCBP2 (see all 174)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 3 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1444898171,2
    --196662103(+) TCTGTA/GCGCTC 2 -- ds50010--------
    rs68066181,2
    C,F,A,H--196662154(+) TCAAAA/GTGGAC 2 -- ds500118Minor allele frequency- G:0.11NA EA NS WA 1810
    rs1446294481,2
    --196662162(+) GACACA/GCGAAT 2 -- ds50010--------
    rs1140765441,2
    F--196662163(+) ACACGC/TGAATT 2 -- ds50011Minor allele frequency- T:0.05WA 118
    rs1151347531,2
    C,F--196662164(+) CACGCG/AAATTG 2 -- ds50011Minor allele frequency- A:0.05WA 118
    rs1884664971,2
    --196662214(+) TGTATC/GCAGAG 2 -- ds50010--------
    rs769020261,2
    F--196662265(+) AACAGG/ACAGCA 2 -- ds50011Minor allele frequency- A:0.01EA 120
    rs67969631,2
    C,F,H--196662275(+) AACAAC/TGATAA 2 -- ut3120Minor allele frequency- T:0.22EA NS NA WA CSA 2122
    rs773873491,2
    F--196662296(+) CTTTAC/TTGAAA 2 -- ut310--------
    rs1922552981,2
    --196662299(+) TATTGA/GAACCT 2 -- ut310--------

    HapMap Linkage Disequilibrium report for NCBP2 (196662273 - 196669468 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 1 variation for NCBP2:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv819558CNV Loss19587683

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing NCBP2
    DNA2.0 Custom Variant and Variant Library Synthesis for NCBP2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 605133    OMIM disorders: --


    Find genes that share disorders with NCBP2           About GenesLikeMe

    Human Genome Epidemiology (HuGE) Navigator: NCBP2 (1 document)

    Export disorders for NCBP2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for NCBP2 gene, integrated from 10 sources (see all 62):
    (articles sorted by number of sources associating them with NCBP2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. A cap-binding protein complex mediating U snRNA export. (PubMed id 7651522)1, 2, 3, 9 Izaurralde E....Mattaj A.W. (Nature 1995)
    2. Identification of the factors that interact with NCBP, an 80 kDa nuclear cap binding protein. (PubMed id 7478990)1, 2, 3 Kataoka N.... Shimura Y. (Nucleic Acids Res. 1995)
    3. A nuclear cap-binding complex binds Balbiani ring pre-mRNA cotranscriptionally and accompanies the ribonucleoprotein particle during nuclear export. (PubMed id 8601613)1, 2, 9 Visa N.... Mattaj I.W. (J. Cell Biol. 1996)
    4. Evidence for a pioneer round of mRNA translation: mRNAs subject to nonsense-mediated decay in mammalian cells are bound by CBP80 and CBP20. (PubMed id 11551508)1, 2, 9 Ishigaki Y.... Maquat L.E. (Cell 2001)
    5. Interaction between the human nuclear cap-binding protein complex and hnRNP F. (PubMed id 9111328)1, 2, 9 Gamberi C.... Mattaj I.W. (Mol. Cell. Biol. 1997)
    6. NMD resulting from encephalomyocarditis virus IRES-directed translation initiation seems to be restricted to CBP80/20-bound mRNA. (PubMed id 18369367)1, 2 Woeller C.F.... Maquat L.E. (EMBO Rep. 2008)
    7. Failsafe nonsense-mediated mRNA decay does not detectably target eIF4E-bound mRNA. (PubMed id 17873884)1, 2 Matsuda D.... Maquat L.E. (Nat. Struct. Mol. Biol. 2007)
    8. Human mRNA export machinery recruited to the 5' end of mRNA. (PubMed id 17190602)1, 2 Cheng H.... Reed R. (Cell 2006)
    9. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    10. eIF4G is required for the pioneer round of translation in mammalian cells. (PubMed id 15361857)1, 2 Lejeune F.... Maquat L.E. (Nat. Struct. Mol. Biol. 2004)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 22916 HGNC: 7659 AceView: NCBP2 Ensembl:ENSG00000114503 euGenes: HUgn22916
    ECgene: NCBP2 Kegg: 22916 H-InvDB: NCBP2

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for NCBP2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for NCBP2 gene:
    Search GeneIP for patents involving NCBP2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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