NCAM2 Gene
protein-coding GIFtS : 57
GCID: GC21 P022370
neural cell adhesion molecule 2
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Aliasesfor NCAM2 gene
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc ,
7 Ensembl ,
8 DME ,
9 miRBase ,
10 fRNAdb , and/or 11 Nature :405,311-319 and CroW21)About This Section Aliases Neural Cell Adhesion Molecule 2 1 2 NCAM211 2 3 N-CAM-22 3 NCAM-22 3 MGC510081 Neural Cell Adhesion Molecule 2 Precursor11
Export aliases for NCAM2 gene to outside databases Previous GC identifers: GC21P019231 GC21P019032 GC21P021292 GC21P007759
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Summariesfor NCAM2 gene (According to Entrez Gene ,
Tocris Bioscience ,
Wikipedia's
Gene Wiki ,
PharmGKB ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
About This Section Entrez Gene summary for NCAM2 : The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein and may function in selective fasciculation and zone-to-zone projection of the primary olfactory axons. (provided by RefSeq, Jul 2008) UniProtKB/Swiss-Prot: NCAM2_HUMAN, O15394 Function : May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactoryaxons
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Genomic Viewsfor NCAM2 gene
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 37) ,
and/or miRBase ,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69) ,
Regulatory elements and Epigenetics data according to
QIAGEN ,
SABiosciences , and/or
SwitchGear Genomics , Whole Chromsome Sequence According to
Nature (Cited Here with Permission) :405,311-319 and CroW21)About This Section
GC21P022370:
GeneLoc
Nature :405,311-319
Start:
22,370,633 bp from pter
8,232,569 bp from centromere
End:
22,915,650 bp from pter
8,490,122 bp from centromere
Size:
545,018 bases
257,554 bases
Orientation:
plus strand
plus strand
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Proteinsfor NCAM2 gene
(According to
1 UniProtKB ,
HORDE ,
neXtProt ,
Ensembl ,
and/or Reactome ,
Modification sites according to 2 PhosphoSitePlus ,
Specific Peptides from DME ,
Protein expression images according to data from SPIRE MOPED and PaxDb ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from
EMD Millipore ,
R&D Systems ,
GenScript ,
Enzo Life Sciences ,
OriGene ,
Novus Biologicals ,
Sino Biological ,
ProSpec , and/or
Uscn ,
Biochemical Assays by
EMD Millipore ,
R&D Systems ,
OriGene ,
GenScript ,
Cell Signaling Technology ,
Enzo Life Sciences , and/or
Uscn ,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene ,
Antibodies by
EMD Millipore ,
R&D Systems ,
GenScript ,
Cell Signaling Technology ,
OriGene ,
Novus Biologicals ,
Thermo Fisher Scientific ,
Abcam , and/or
Uscn )
About This Section UniProtKB/Swiss-Prot: NCAM2_HUMAN, O15394 (See
protein sequence )Recommended Name: Neural cell adhesion molecule 2 precursor Size : 837 amino acids; 93046 Da
Subcellular location : Cell membrane; Single-pass type I membrane protein
6/9 PDB 3D structures from and Proteopedia for NCAM2 (see all 9 ):2DOC (3D)
  2JLL (3D)
  2KBG (3D)
  2V5T (3D)
  2VAJ (3D)
  2WIM (3D)
 
Secondary accessions : A8MQ06 Q7Z7F2Explore the universe of human proteins at neXtProt for NCAM2: NX_O15394 Post-translational modifications:
View modification sites using PhosphoSitePlus 2 View neXtProt modification sites for NX_O15394 NCAM2 Protein expression data from MOPED and PaxDb : About this image
REFSEQ proteins: NP_004531.2 ENSEMBL proteins: ENSP00000383392 ENSP00000284894 ENSP00000441887 Human Recombinant Protein Products: Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view) : About this table
NCAM2 for ontologies About GeneDecksing NCAM2 Antibody Products: Assay Products for NCAM2:
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Protein
Domains / Familiesfor NCAM2 gene (According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
About This Section
NCAM2 for domains About GeneDecksing 5/6 InterPro domains/families (see all 6 ):
Graphical View of Domain Structure for InterPro Entry O15394 ProtoNet protein and cluster: O15394
2 Blocks protein families : IPB003598 Immunoglobulin C-2 type IPB009138 Neural cell adhesion molecule signature UniProtKB/Swiss-Prot: NCAM2_HUMAN, O15394 Similarity : Contains 2 fibronectin type-III domainsSimilarity : Contains 5 Ig-like C2-type (immunoglobulin-like) domains
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Functionfor NCAM2 gene
(According to 1 UniProtKB ,
Genatlas ,
LifeMap Discovery™ ,
IUBMB , and/or
2 DME ,
Human phenotypes from GenomeRNAi ,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences ,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene ,
RNAi from
EMD Millipore ,
siRNAs from
OriGene ,
QIAGEN ,
microRNA from QIAGEN ,
Gene Editing from DNA2.0 ,
Clones from EMD Millipore ,
OriGene ,
SwitchGear Genomics ,
GenScript ,
Sino Biological ,
DNA2.0 ,
and Vector BioLabs ,
Cell Lines from GenScript ,
LifeMap BioReagents ,
In Situ Hybridization Assays from Advanced Cell Diagnostics ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene .)
About This Section Function Summary: UniProtKB/Swiss-Prot: NCAM2_HUMAN, O15394 Function : May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactoryaxons
Genatlas biochemistry entry for NCAM2 : cell adhesion molecule,neural,2,immunoglobulin superfamily Clone Products: Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for NCAM2 (see all 4 ) OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for NCAM2OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector: NCAM2 (NM_004540 ) Browse Sino Biological Human cDNA Clones DNA2.0 Custom Codon Optimized Gene
Synthesis Service for NCAM2 Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat NCAM2
In Situ Assay Products: Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NCAM2
1 GenomeRNAi human phenotype for NCAM2 :Animal Models: Mouse knock-out Ncam2 tm1Mom for NCAM2 3 MGI mutant phenotypes (inferred from 2 alleles ) (MGI details for Ncam2) :
NCAM2 for phenotypes About GeneDecksing
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Pathways & Interactionsfor NCAM2 gene
(Pathways according to
EMD Millipore ,
R&D Systems ,
Cell Signaling Technology ,
KEGG ,
PharmGKB ,
BioSystems ,
Reactome ,
Tocris Bioscience ,
GeneGo (Thomson Reuters) ,
QIAGEN ,
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Interaction Networks according to
SABiosciences ,
and/or STRING ,
Interactions according to 1 UniProtKB ,
2 MINT ,
3 I2D , and/or
4 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene) .
About This Section Unified GeneCards pathways   About this table See pathways by source Super-pathway contained gene-specific pathways 1 Prion diseases 2 Cell adhesion molecules (CAMs)
Pathway sources See GeneCards unified pathways Show all pathways 2
Kegg Pathways (Kegg details for NCAM2) :
NCAM2 for pathways About GeneDecksing Interactions: Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for NCAM2 STRING Interaction
Network Preview (showing 5 interactants - click image to see more details)5/98 Interacting proteins for NCAM2 (O15394 3 ENSP00000383392 4 ) via UniProtKB, MINT, STRING , and/or I2D (see all 98 )About this table Gene Ontology (GO): 2 biological process terms (GO ID links to tree view) : About this table
NCAM2 for ontologies About GeneDecksing
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Drugs & Compoundsfor NCAM2 gene (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
EMD Millipore , Tocris Bioscience
HMDB ,
BitterDB , and/or
Novoseek , and Drugs according to
DrugBank ,
Enzo Life Sciences , and/or
PharmGKB , with drugs/clinical trials/news
search links to CenterWatch )
About This Section Browse Tocris compounds for NCAM2 Search CenterWatch for drugs/clinical trials and news about NCAM2
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Transcriptsfor NCAM2 gene (Secondary structures according to
fRNAdb ,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
transcript ids from Ensembl
with links to UCSC ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from
EMD Millipore ,
siRNAs from
OriGene ,
QIAGEN ,
shRNA from
OriGene ,
microRNA from QIAGEN ,
Tagged/untagged cDNA clones from
OriGene ,
SwitchGear Genomics ,
GenScript ,
DNA2.0 ,
Vector BioLabs ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN
)About This Section REFSEQ mRNAs for NCAM2 gene: NM_004540.3 Unigene Cluster for NCAM2:
Neural cell adhesion molecule 2 Hs.473450 [show with all ESTs ] Unigene Representative Sequence: NM_004540 7 Ensembl transcripts including schematic representations, and UCSC links where relevant : ENST00000400546 (uc002yld.2 uc011acb.2 ) ENST00000508782 ENST00000486367 ENST00000461281 ENST00000484983 ENST00000284894 ENST00000535285 (uc011acc.2 )Clone Products: OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for NCAM2 (see all 4 ) OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for NCAM2OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector: NCAM2 (NM_004540 ) DNA2.0 Custom Codon Optimized Gene
Synthesis Service for NCAM2 Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat NCAM2
Additional cDNA sequence: AK299054.1 AK302870.1 AL137344.1 BC036088.1 BC052946.1
4 DOTS entries : DT.310937 DT.121136095
DT.40119178 DT.95357462 24/85 AceView cDNA sequences (see all 85 ):
BM835913 AA234378 AA382726 BM729225 BM666325 AL120485 AL138049 BU580655 BM682143 F06760 BC052946 BQ717550 AL120407 NM_004540 Z38354 AA709271 F03927 Z39816 BP357558 BP371109 AI207079 Z42071 AL137344 BM711312 GeneLoc Exon Structure
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Expression for NCAM2 gene
(RNA expression data according to
H-InvDB ,
NONCODE ,
miRBase , and
RNAdb ,
Expression images according to data from
BioGPS ,
Illumina Human BodyMap , and
CGAP
SAGE ,
Sets of similar genes according to GeneDecks ,
in vivo and in vitro expression data from LifeMap Discovery™ ,
plus additional links to
Genevestigator , and/or
SOURCE , and/or
BioGPS , and/or
UniProtKB ,
PCR Arrays from
SABiosciences ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN ,
In Situ Hybridization Assays from Advanced Cell Diagnostics )
About This Section NCAM2 expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this image BioGPS CGAP TAG: TGTATGAGTC
About this image NCAM2 expression in embryonic tissues and stem cells Expression by the Database of Embryonic development, Stem cell research, and
Regenerative medicine About this table See NCAM2 Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for NCAM2 SOURCE GeneReport for Unigene cluster: Hs.473450 UniProtKB/Swiss-Prot: NCAM2_HUMAN, O15394 Tissue specificity : Expressed most strongly in adult and fetal brain SABiosciences Custom PCR Arrays for NCAM2 Primer Products: OriGene genome-wide validated SYBR primer pairs in human , mouse , rat for NCAM2Browse OriGene validated miRNA SYBR primer pairs SABiosciences RT2 qPCR Primer Assay in human , mouse , rat NCAM2 QIAGEN QuantiTect SYBR Green Assays in human , mouse , rat NCAM2 QIAGEN QuantiFast Probe-based Assays in human , mouse , rat NCAM2 In Situ Assay Products: Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NCAM2
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Orthologsfor NCAM2 gene
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
,
5 MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase ,
and/or
6 Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam )
About This Section
This gene was present in the common ancestor of animals.
Orthologs for NCAM2 gene from 5/18 species (see all 18 ) About this table
Organism
Taxonomic classification
Gene
Description
Human Similarity
Orthology Type
Details
chicken (Gallus gallus)
Aves
NCAM21
neural cell adhesion molecule 2
81.66(n) 87.29(a)
 
427968 XM_425540.3 XP_425540.3
lizard (Anolis carolinensis)
Reptilia
NCAM26
--
78(a)
1 ↔ 1
3(150962412-151137572)
tropical clawed frog (Xenopus tropicalis)
Amphibia
Str.118522
Transcribed sequence with moderate similarity to protein more
77.47(n)
 
BX725298.1
zebrafish (Danio rerio)
Actinopterygii
ncam22
neural cell adhesion molecule 2
72.44(n)
 
114441 AY029403.1
fruit fly (Drosophila melanogaster)
Insecta
Fas21 , 3
homophilic cell adhesion3 Fasciclin 21
29(a) 3 41.17(n) 1 30.2(a) 1
 
31364 1 NM_080327.2 1 NP_525066.1 1
ENSEMBL Gene Tree for NCAM2 (if available)TreeFam Gene Tree for NCAM2 (if available)
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Paralogsfor NCAM2 gene (Paralogs according to
1 HomoloGene , 2 Ensembl , and 3 SIMAP , Pseudogenes according to 4 Pseudogene.org Build 68)About This Section Paralogs for NCAM2 gene NTM 2 NEGR1 2 IGLON5 2 LSAMP 2 NCAM1 2 OPCML 2 4 SIMAP similar genes for NCAM2 using alignment to 5 protein entries: NCAM2_HUMAN (see all proteins ):NCAM1 K-SAM CNTN4 HMCN2
NCAM2 for paralogs About GeneDecksing
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Genomic Variantsfor NCAM2 gene (SNPs/Variants according to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE ,
UniProtKB , and
DNA2.0 ,
Linkage Disequilibrium by HapMap ,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants , Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB) , Blood group antigen gene mutations by BGMUT ,
Resequencing Primers from QIAGEN ,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences )
About This Section
Genomic Data Transcription Related Data Allele Frequencies SNP ID Valid Clinical significance Chr 21 pos Sequence # AA Chg Type More # Allele freq Pop Total sample More
HapMap Linkage Disequilibrium report for NCAM2 (22370633 - 22620633 bp, first 250kb of NCAM2)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV): 7 variations for NCAM2 6 CNVs : 9831 59040 0330 23637 9325 103050 1 Indel : 33721
SABiosciences Cancer Mutation PCR Assays
QIAGEN SeqTarget long-range PCR primers in human , mouse , rat for resequencing NCAM2
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Disorders
/ Diseasesfor NCAM2 gene
(in which this Gene is Involved, According to MalaCards ,
OMIM, UniProtKB ,
the University of Copenhagen DISEASES
database ,
Genatlas , GeneTests ,
GAD ,
HuGE Navigator ,
and/or TGDB .)
About This Section
NCAM2 for disorders About GeneDecksing
OMIM gene information: 602040
OMIM disorders : --7 diseases for NCAM2 : About MalaCards chronic interstitial cystitis prion disease down syndrome breast cancer obesity prostatitis neuronitis Human Genome Epidemiology (HuGE) Navigator: NCAM2 (5 documents) Export disorders for NCAM2 gene to outside databases
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Publicationsfor NCAM2 gene (in
PubMed .
Associations of this gene to articles via
1 Entrez Gene ,
2 UniProtKB/Swiss-Prot ,
3 HGNC ,
4 GAD ,
5 PharmGKB ,
6 HMDB ,
7 DrugBank ,
8 UniProtKB/TrEMBL ,
9 Novoseek , and/or
10 fRNAdb )
About This Section PubMed articles for NCAM2 gene, integrated from 9 sources (see all 19 ): (articles sorted by number of sources associating them with NCAM2) Utopia : connect your pdf to the dynamic world of online information
Cloning of a novel human neural cell adhesion molecule gene (NCAM2) that maps to chromosome region 21q21 and is potentially involved in Down syndrome. (PubMed id 9226371) 1 , 2 , 3 Paoloni-Giacobino A.... Antonarakis S.E. (1997) Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry. (PubMed id 12754519) 1 , 2 Zhang H.... Aebersold R. (2003) The DNA sequence of human chromosome 21. (PubMed id 10830953) 1 , 2 Hattori M....Yaspo M.L. (2000) Genome-wide association for abdominal subcutaneous and visceral adipose reveals a novel locus for visceral fat in women. (PubMed id 22589738) 1 Fox C.S....Borecki I.B. (2012) Neural cell adhesion molecule 2 as a target molecule for prostate and breast cancer gene therapy. (PubMed id 21214674) 1 Takahashi S....Hamada H. (2011) Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983) 1 Kim W....Gygi S.P. (2011) Structural model and trans-interaction of the entire ectodomain of the olfactory cell adhesion molecule. (PubMed id 21300289) 1 Kulahin N....Gajhede M. (2011) A genome-wide association study on obesity and obesit y-related traits. (PubMed id 21552555) 1 Wang K....Price R.A. (2011) Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614) 1 Rose J.E....Uhl G.R. (2010) Genome-wide association reveals genetic effects on hu man AI^42 and I8 protein levels in cerebrospinal fluids: a case control study. (PubMed id 20932310) 1 Han M.R....Wang L.S. (2010)
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External Searches for NCAM2 gene
(in PubMed ,
OMIM , and NCBI Bookshelf ) About This Section
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Genome Databases showing NCAM2 gene
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
Kegg ,
and/or
H-InvDB )
About This Section
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Other Databases showing NCAM2 gene
(According to HUGE )
About This Section --
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Specialized Databases showing NCAM2 gene (According to PharmGKB ,
ATLAS , HORDE , IMGT , LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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About This Section Patent Information for NCAM2 gene: Search GeneIP for patents involving NCAM2 GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
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Productsfor NCAM2 gene (Antibodies, recombinant proteins, and assays from EMD Millipore , R&D Systems , OriGene , QIAGEN , GenScript , Cell Signaling Technology , SABiosciences , Novus Biologicals , Sino Biological , Enzo Life Sciences , Abcam , ProSpec , Uscn , Thermo Fisher Scientific , Gene Editing from DNA2.0 , Clones from EMD Millipore , OriGene , GenScript , Sino Biological , DNA2.0 , SwitchGear Genomics , Vector BioLabs , Cell lines from GenScript and LifeMap BioReagents , PCR Arrays from SABiosciences , Drugs and/or compounds from EMD Millipore , Tocris Bioscience , and/or
Enzo Life Sciences ), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
Browse OriGene Antibodies OriGene shRNA RFP for NCAM2 OriGene 29mer shRNA kits in GFP-retroviral vector in human , mouse , rat for NCAM2 OriGene genome-wide validated SYBR primer pairs in human , mouse , rat for NCAM2 Browse OriGene Protein Over-expression Lysates Browse OriGene Fluorogenic Cell Assay Kits OriGene siRNA for NCAM2 OriGene 3'-UTR Clone for NCAM2 OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for NCAM2 OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for NCAM2 Browse OriGene GFP tagged cDNA clones in CMV expression vector Browse OriGene MicroRNA Expression Plasmids Browse OriGene basic RS shRNAs Browse OriGene validated miRNA SYBR primer pairs Browse OriGene full length recombinant human proteins expressed in human HEK293 cells OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling OriGene Custom Antibody Services for NCAM2 OriGene Custom Protein Services for NCAM2 OriGene Custom Immunoassay Development
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat NCAM2 QIAGEN SeqTarget long-range PCR primers in human , mouse , rat for resequencing NCAM2 QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human , mouse , rat NCAM2 QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human , mouse , rat NCAM2 QIAGEN QuantiFast Probe-based Assays in human , mouse , rat NCAM2 QIAGEN QuantiTect SYBR Green Assays in human , mouse , rat NCAM2
Search Tocris compounds for NCAM2
NCAM2 Proteins, Antibodies, CLIAs, and ELISAs
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NCAM2
Search ThermoFisher Antibodies for NCAM2
Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat NCAM2
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Aliases for NCAM2
Databases for NCAM2
Disorders / Diseases for NCAM2
Domains / Families for NCAM2
Drugs / Compounds for NCAM2
Expression for NCAM2
Function for NCAM2
Genomic Views for NCAM2
Intellectual Property for NCAM2
Orthologs for NCAM2
Paralogs for NCAM2
Pathways / Interactions for NCAM2
Products for NCAM2
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Search Box for NCAM2
Summaries for NCAM2
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