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NAT8 Gene

protein-coding   GIFtS: 49

GC02M073779
N-acetyltransferase 8 (GCN5-related, putative)
(Previous name: N-acetyltransferase 8 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
CML1 2, 3
EC 2.3.1.- 3
GLA 1, 2, 3
Hcml1 1, 2
TSC501 1, 2, 3
TSC510 2, 5
Descriptions
Camello-like protein 1 2, 3
N-acetyltransferase 8 1, 2
N-acetyltransferase 8 (GCN5-related, putative) 2
N-acetyltransferase 8 (camello like) 2
kidney- and liver-specific gene product 2
External Ids
HGNC: 180691
Entrez Gene: 90272
UniProtKB: Q9UHE53
Ensembl: ENSG000001440357
Search outside databases for aliases for NAT8 gene

Previous GC identifers: GC02M073877 GC02M074078 GC02M073842

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for NAT8:
This gene, isolated using the differential display method to detect tissue-specific genes, is
specifically expressed in kidney and liver. The encoded protein shows amino acid sequence
similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and
gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is
found in a cluster with this gene. [provided by RefSeq]

UniProtKB/Swiss-Prot: NAT8_HUMAN, Q9UHE5
Function: Plays a role in regulation of gastrulation

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NAT8 gene  

Entrez Gene cytogenetic band: 2p13.1-p12   Ensembl cytogenetic band:  2p13.2   HGNC cytogenetic band: 2p13.2

NAT8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02M073779:     (about GC identifiers)

Start:
73,721,358 bp from pter
End:
73,723,045 bp from pter
Size:
1,688 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000002.10  NT_022184.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: NAT8_HUMAN, Q9UHE5 (See protein sequence)
Recommended Name: Probable N-acetyltransferase 8  
Size: 227 amino acids; 25619 Da
Subcellular location: Membrane; Multi-pass membrane protein (Potential)
Secondary accessions: O75839 Q6LEU4 Q96QI8 Q9UQ17

REFSEQ proteins: NP_003951.3  

ENSEMBL proteins: 
ENSP00000272425 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Browse Abcam for Recombinant Proteins
Human Recombinant Proteins from Abnova (NAT8)
              OriGene Purified Recombinant Human Protein: NAT8 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016020 membrane IEA--
GO:0016021 integral to membrane NAS11397015
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Antibodies for NAT8: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for NAT8

Assays for NAT8: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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2 InterPro domains/families:
 IPR000182 GCN5-rel_AcTrfase
 IPR016181 Acyl_CoA_acyltransferase


   GeneDecks  NAT8 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q9UHE5

ProtoNet protein and cluster: Q9UHE5

1 Blocks protein family: IPB000182 GCN5-related N-acetyltransferase

UniProtKB/Swiss-Prot: NAT8_HUMAN, Q9UHE5
Similarity: Belongs to the camello family
Similarity: Contains 1 N-acetyltransferase domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NAT8)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003960

              Applied Biosystems Silencer® siRNAs for NAT8

              Sigma-Aldrich siRNA and siRNA Panels for NAT8  
                     Sigma-Aldrich shRNA for NAT8  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for NAT8
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_003960
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_003960
                                 untagged cDNA clone in CMV expression vector: NM_003960 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_003960

UniProtKB/Swiss-Prot: NAT8_HUMAN, Q9UHE5
Function: Plays a role in regulation of gastrulation
Enzyme Number (IUBMB): EC 2.3.1.- 

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0008080 N-acetyltransferase activity NAS11397015
GO:0008415 acyltransferase activity IEA--
GO:0016740 transferase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


  --

4 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0001702 gastrulation with mouth forming second IMP11397015
GO:0007275 multicellular organismal development IEA--
GO:0008152 metabolic process IEA--
GO:0042493 response to drug TAS9852678
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for NAT8

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NAT8)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003960

              Sigma-Aldrich siRNA and siRNA Panels for NAT8  
                     Sigma-Aldrich shRNA for NAT8  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_003960  

REFSEQ mRNAs for NAT8 gene: 

NM_003960.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_003960  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_003960
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_003960
                                 untagged cDNA clone in CMV expression vector: NM_003960 

Additional cDNA sequence: 

AB013094.1 AB019551.1 AF187813.1 BC012626.1 CR407610.1 

4 DOTS entries:

DT.100812576  DT.100812571  DT.95372741  DT.75163459 

24/65 AceView cDNA sequences (see all 65 ):

AA985528 CB161536 BI761242 AI431825 BM924554 AA581767 AI283636 N71377 
AI174771 N71326 BG401637 BI759077 BI763593 BV199736 BG426653 BG563833 
AL698327 BG427673 AB019551 N75734 NM_003960 N58170 BI762640 BI759313 

highest scoring ESTs for NAT8:

AB013094 AB019551 AF187813 AI174771 AV650091 BC012626 BF104866 BF104869 BG400118 BG400318 

Unigene Cluster for NAT8:

N-acetyltransferase 8 (GCN5-related, putative)
Hs.14637  [show with all ESTs]
Unigene Representative Sequence: BQ644205


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000272425  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

NAT8 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for NAT8

1 / 2 / 3

5 probe-sets matching NAT8 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
38128_at2, 3 U95-A 2 1.00 0.81 1.00 1.00 AB013094 1.00 1.00 1.00 1

210289_at2, 3 U133-A 1 1.00 1.00 -- -- AB013094 1.00 1.00 1.00 1

206963_s_at2, 3 U133-A 3 1.00 0.39 -- -- NM_016347 0.20 0.33 0.27 2

210289_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

206963_s_at2 U133Plus2 3 1.00 0.39 -- -- -- -- -- -- --
GeneDecks  NAT8 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: GGCTTCAAGA

SOURCE GeneReport for Unigene cluster: Hs.14637

Expression variation in blood from EXPOLDB for NAT8

UniProtKB/Swiss-Prot: NAT8_HUMAN, Q9UHE5
Tissue specificity: Expression is restricted to liver and kidney

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for NAT8 gene from 3 species
Organism Gene Locus Description Human
Similarity
NCBI accessions
chimpanzee
(Pan troglodytes)
NAT81   -- N-acetyltransferase 8 (GCN5-related, putative) 98.83(n)
99.12(a)
470406  XM_001151030.1  XP_001151030.1 
rat
(Rattus norvegicus)
Cml41   -- camello-like 4 72.22(n)
64.41(a)
64570  NM_022635.1  NP_072157.1 
mouse
(Mus musculus)
Cml41   -- camello-like 4 71.6(n)
61.88(a)
68396  NM_023455.2  NP_075944.1 
About this table        Species with no ortholog for NAT8

ENSEMBL Gene Tree for NAT8
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for NAT8 gene
ENSG000002048722  NAT8L2  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/73 NCBI SNPs in NAT8 are shown (see all 73 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 17)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 2 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
--
rs22805071,2
A,C,F,H,O73723066(-) GCAATG/AAGGCA 1 -- ng317Minor allele frequency- A:0.15NA EU EA WA 542
rs43286561,2
A,C,F,H73720968(+) CCCTGT/GAGTTA 1 -- ng513Minor allele frequency- G:0.23EU EA 276
rs22805061,2
A,C,F,H73723174(-) AGGTCA/GTTCCC 1 -- ng315Minor allele frequency- G:0.30EA EU WA 1888
--
rs135381,2
A,C,F,H73721836(-) TCTCTT/CTGTGG 1 S/F mis1 ese313Minor allele frequency- C:0.28MN NA EU EA WA 1239
rs48529511,2
A,C,F,H73723304(+) TCGGTG/CCCTGT 1 -- ng3111Minor allele frequency- C:0.45NA EU EA WA 970
rs134245611,2
C,F,H73721954(+) ACGCTC/TACTCA 1 E/K mis18Minor allele frequency- T:0.04EU EA WA 838
--
rs44551781,2
C,F,H73721453(+) ATCACA/GTGGGA 1 -- ut3111Minor allele frequency- G:0.03EU EA WA NA 978
--
rs18796651,2
A,C,F73720977(-) AATCCA/CCTCTA 1 -- ng511Minor allele frequency- C:0.03MN 184
--
rs18796621,2
A,C,F73721405(-) GTCTTT/CTTGAT 1 -- ut311Minor allele frequency- C:0.42MN 184
--
rs48529541,2
A,C73723518(+) CCAACC/TCCCAT 1 -- ng310--------
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HapMap Linkage Disequilibrium images for NAT8 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 606716

1 PharmGKB disease relationship for NAT8 gene
Disease PharmGKB Relations PubMed IDs for articles supporting these relationships
Leukemia, Lymphocytic, Acute, L1FA  GN  12086872
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Human Genome Epidemiology Navigator: NAT8 (1 document)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/14 PubMed articles for NAT8 gene (see all 14 ):
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 9027 HGNC: 18069 AceView: NAT8 Ensembl:ENSG00000144035 euGenes: HUgn9027
ECgene: NAT8 H-InvDB: NAT8
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for NAT8:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for NAT8-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for NAT8 Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for NAT8 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Search Tocris compounds for NAT8




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Search Novus for antibodies for NAT8

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