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NAT12 Gene

protein-coding GIFtS: 47

GC14P056928

N-acetyltransferase 12 (GCN5-related, putative)
(Previous names: chromosome 14 open reading frame 35, N-acetyltransferase 12 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: C14orf35)

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
C14orf35 ^², ³
EC 2.3.1.- ^³
FLJ35355 ^¹, ²
MAK3 ^¹, ², ³
MGC141884 ^²
MGC141885 ^²
Mak3p ^¹, ²

Descriptions
N-acetyltransferase 12 ^¹, ², ³
N-acetyltransferase 12 (GCN5-related, putative) ^²
chromosome 14 open reading frame 35 ^¹
putative N-acetyltransferase ^²

External Ids
HGNC: 19844¹
Entrez Gene: 122830²
UniProtKB: Q147X3³
Ensembl: ENSG00000139977⁷

Search outside databases for aliases for NAT12 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: MAK3_HUMAN, Q147X3

Function: Probable N-acetyltransferase (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the NAT12 gene

Entrez Gene cytogenetic band: 14q23.1 Ensembl cytogenetic band: 14q23.1 HGNC cytogenetic band: 14q22.2

NAT12 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 14 GeneLoc Exon Structure

GeneLoc location for GC14P056928: (about GC identifiers)

Start:	56,927,024 bp from pter
End:	56,947,636 bp from pter
Size:	20,613 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000014.7 NT_026437.11

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: MAK3_HUMAN, Q147X3 (See protein sequence)

Recommended Name: N-acetyltransferase MAK3 homolog

Size: 362 amino acids; 39320 Da

Secondary accessions: Q0IIN2

Alternative splicing: 2 isoforms: Q147X3-1 Q147X3-2

Post-translational modifications:

View phosphorylation sites using PhosphoSite²

REFSEQ proteins: NP_001011713.2

ENSEMBL proteins:

ENSP00000298406 ENSP00000378676

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (MAK3)
	Browse Abnova for Human Recombinant Proteins

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

Antibodies for NAT12:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for NAT12
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (MAK3), each with their Abpromise^SM
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for NAT12

Assays for NAT12:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q147X3

ProtoNet protein and cluster: Q147X3

UniProtKB/Swiss-Prot: MAK3_HUMAN, Q147X3

Similarity: Belongs to the acetyltransferase family. MAK3 subfamily

Similarity: Contains 1 N-acetyltransferase domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (NAT12)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): XM_058661

Applied Biosystems Silencer® siRNAs for NAT12

Sigma-Aldrich siRNA for NAT12

Sigma-Aldrich shRNA for NAT12

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_001011713
Myc/DDK tagged cDNA clone in CMV expression vector: NM_001011713
untagged cDNA clones in CMV expression vector (see all 2): NM_001011713

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_001011713

UniProtKB/Swiss-Prot: MAK3_HUMAN, Q147X3

Function: Probable N-acetyltransferase (By similarity)

Enzyme Number (IUBMB): EC 2.3.1.-

1 MGI phenotypic allele for Nat12 (no phenotypes)

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0008080	N-acetyltransferase activity	IEA	--
GO:0008415	acyltransferase activity	IEA	--
GO:0016740	transferase activity	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

1 Interacting protein for NAT12 (ENSP00000298406³) via UniProtKB, MINT, and/or STRING

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
MAK10	ENSP00000354972³	STRING (score=.73)

About this table

1 Gene Ontology (GO) biological process term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0008152	metabolic process	IEA	--

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for NAT12

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (NAT12)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): XM_058661

Sigma-Aldrich siRNA for NAT12

Sigma-Aldrich shRNA for NAT12

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_001011713

REFSEQ mRNAs for NAT12 gene:

NM_001011713.2

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_001011713

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001011713
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001011713
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001011713

Additional cDNA sequence:

AK024885.1 AK025749.1 AK092674.1 AK296372.1 AK304257.1 AK307348.1 BC048983.1 BC118589.1

BC122557.1 BX648700.1

8 DOTS entries:

DT.415321 DT.404156 DT.441829 DT.102824364 DT.100754625 DT.95106614 DT.97823552 DT.120753996

24/156 AceView cDNA sequences (see all 156 ):

BI964191 BF061915 CB242587 AI298002 W60557 AA442141 BM313443 AA608632

BM704461 CA941313 AA435565 BE327045 AI803224 BQ959365 AI687432 BU181327

CA941607 AA393159 BQ938170 AI753449 CA437004 N46008 H67235 AA541417

highest scoring ESTs for NAT12:

AA132886 AA248068 AA351408 AA442141 AA534210 AA628624 AI452950 AI522133 AI888124 AK024885

Unigene Cluster for NAT12:

N-acetyltransferase 12 (GCN5-related, putative)

Hs.165465 [show with all ESTs]

Unigene Representative Sequence: NM_001011713

GeneLoc Exon Structure

4 Alternative Splicing Database (ASD) splice patterns (SP) for NAT12

ExUns:	1a	·	1b	·	1c	^	2a	·	2b	·	2c	^	3	^	4	^	5a	·	5b	·	5c
SP1:
SP2:							-		-		-
SP3:											-
SP4:							-		-		-				-

About this scheme

ECgene alternative splicing isoforms for NAT12

2 Ensembl transcripts including schematic representations:

ENST00000298406 ENST00000395257

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

NAT12 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for NAT12

¹ / ² / ³

5 probe-sets matching NAT12 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
88747_r_at²^{, 3}	U95-D	1	1.00	1.00	0.47	0.72	AI393257	0.60	1.00	0.82	1
47959_at²^{, 3}	U95-B	1	1.00	1.00	0.91	1.14	AI522133	0.80	1.00	0.91	1
48574_at²^{, 3}	U95-B	1	1.00	1.00	0.88	1.15	AA534210	0.80	1.00	0.91	1
225679_at²^{, 3}	U133-B	1	1.00	1.00	--	--	AA534210	0.80	1.00	0.91	1
225679_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: ACATCACTAA

SOURCE GeneReport for Unigene cluster: Hs.165465

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for NAT12 gene from 5/16 species (see all 16 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	NAT12¹	--	N-acetyltransferase 12 (GCN5-related, putative)	95.29(n) 94.18(a)	612880 XM_846834.1 XP_851927.1
chimpanzee (Pan troglodytes)	NAT12¹	--	N-acetyltransferase 12 (GCN5-related, putative)	99.63(n) 99.17(a)	452931 XM_509970.2 XP_509970.2
cow (Bos taurus)	NAT12¹	--	N-acetyltransferase 12 (GCN5-related, putative)	94.6(n) 93.3(a)	533922 XM_613489.3 XP_613489.2
rat (Rattus norvegicus)	RGD1559923¹	--	similar to chromosome 14 open reading frame 35	90.05(n) 88.73(a)	498489 XM_573749.2 XP_573749.1
mouse (Mus musculus)	Nat12^{1, 5}	14⁵	N-acetyltransferase 12^{1, 5}	89.04(n)¹ 87.64(a)¹	70646¹ NM_001081430.1¹ NP_001074899.1¹ AA139112⁵ AA615171⁵ (see all 19)

About this table Species with no ortholog for NAT12

ENSEMBL Gene Tree for NAT12

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/69 NCBI SNPs in NAT12 are shown (see all 69 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 39)

AB	SNP ID	Valid	Chr 14 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs11625443^1,2	A,C,F,H	56946328(+)	`GTTCCC/TTAAAA`	1	--	ut3¹ ese³		7		EU EA WA NA	552
	rs1058010^1,2	C,F,H,O	56948632(+)	`AAACTA/GCTTTA`	1	--	ut3¹		12		MN NA EU EA WA	1160
	rs11620639^1,2	A,C,F,H	56946018(+)	`CATCCA/GCACAG`	1	--	ut3¹ ese³		7		NA EU EA WA	550
	rs3825621^1,2	A,C,F,H	56946595(+)	`ATCTCC/TAGTTT`	1	--	ut3¹ ese³		12		EA EU WA NA	2270
	rs3742580^1,2	C,F,H	56946946(+)	`TGCTAC/TGCTGC`	1	--	ut3¹		8		EA NA EU WA	2046
--	rs11158159^1,2	A,C	56926915(+)	`AGCCCC/GCAGGA`	1	--	ng5¹		0	--	--	--	--
--	rs8004627^1,2	A,C	56925484(+)	`taccaC/Tgcccg`	1	--	ng5¹		0	--	--	--	--
--	rs4362296^1,2	H	56933328(+)	`CCAAAT/AACAGG`	1	N/Y	mis¹ ese³		4		EU EA WA	394
	rs1057737^1,2	A,C,F,H	56930441(-)	`GGTTTC/TTAATC`	1	--	int¹		13		NS MN EU EA WA NA	836
	rs2152360^1,2	A,C,F,H	56936376(+)	`GTTTAG/ATATTT`	1	--	int¹		2		EU	228

About this table

HapMap Linkage Disequilibrium images for NAT12 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

6 PubMed articles for NAT12 gene:

Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. (PubMed id 18691976)⁴ Daub H.... Mann M. (2008)
A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648)⁴ Dephoure N.... Gygi S.P. (2008)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)⁴ Gerhard D.S....Malek J. (2004)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)³ Ota T....Sugano S. (2004)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )⁴ Aoki H.... Hino O. (2000)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 122830	HGNC: 19844	AceView: C14orf35	Ensembl:ENSG00000139977	euGenes: HUgn122830
ECgene: NAT12	H-InvDB: NAT12

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for NAT12
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for NAT12:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for NAT12-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for NAT12		Antibodies for NAT12
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for NAT12		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

	Search for Recombinant Proteins
	Search for Antibodies
	Search for Chimera RNAi
	Custom Service for Mouse Mab
	Custom Service for Rabbit Pab from Full-length Protein

Search for Antibodies & Assays

	Recombinant Proteins (MAK3)
	Antibodies (MAK3)

Search OriGene for NAT12

Search Tocris compounds for NAT12

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Search Novus for antibodies for NAT12

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NAT12 Gene

protein-coding GIFtS: 47

Aliases &
Descriptions
for NAT12

Summaries
for NAT12

Genomic Location
for NAT12

Proteins
for NAT12

Protein Domains/
Families
for NAT12

Gene Function
for NAT12

Pathways & Interactions
for NAT12

Drugs & Compounds
for NAT12

Transcripts
for NAT12

Expression
for NAT12

Orthologs
for NAT12

Paralogs
for NAT12

SNPs/Variants
for NAT12

Disorders & Mutations
for NAT12

Medical News
for NAT12

Publications
for NAT12

Search for NAT12

Genome Databases showing NAT12

Other Databases showing NAT12

Specialized Databases showing NAT12

Licensable Technologies
for NAT12

Services
for NAT12

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

NAT12 Gene

protein-coding GIFtS: 47

Aliases & Descriptionsfor NAT12

Summariesfor NAT12

Genomic Locationfor NAT12

Proteinsfor NAT12

Protein Domains/Familiesfor NAT12

Gene Functionfor NAT12

Pathways & Interactionsfor NAT12

Drugs & Compoundsfor NAT12

Transcriptsfor NAT12

Expression for NAT12

Orthologsfor NAT12

Paralogsfor NAT12

SNPs/Variantsfor NAT12

Disorders & Mutationsfor NAT12

Medical Newsfor NAT12

Publicationsfor NAT12

Search for NAT12

Genome Databases showing NAT12

Other Databases showing NAT12

Specialized Databases showing NAT12

Licensable Technologiesfor NAT12

Servicesfor NAT12

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for NAT12

Summaries
for NAT12

Genomic Location
for NAT12

Proteins
for NAT12

Protein Domains/
Families
for NAT12

Gene Function
for NAT12

Pathways & Interactions
for NAT12

Drugs & Compounds
for NAT12

Transcripts
for NAT12

Expression
for NAT12

Orthologs
for NAT12

Paralogs
for NAT12

SNPs/Variants
for NAT12

Disorders & Mutations
for NAT12

Medical News
for NAT12

Publications
for NAT12

Licensable Technologies
for NAT12

Services
for NAT12