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NARS2 Gene

protein-coding   GIFtS: 50

GC11M077825
asparaginyl-tRNA synthetase 2, mitochondrial (putative)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
AsnRS 3
EC 6.1.1.22 3
FLJ23441 1, 2
SLM5 1, 2
Descriptions
Asparagine--tRNA ligase 3
asparagine tRNA ligase 2, mitochondrial (putative) 2
asparaginyl-tRNA synthetase 2, mitochondrial 2
asparaginyl-tRNA synthetase 2, mitochondrial (putative) 2
External Ids
HGNC: 262741
Entrez Gene: 797312
UniProtKB: Q96I593
Ensembl: ENSG000001375137
Search outside databases for aliases for NARS2 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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  --
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NARS2 gene  

Entrez Gene cytogenetic band: 11q14.1   Ensembl cytogenetic band:  11q14.1   HGNC cytogenetic band: 11q14.1

NARS2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11M077825:     (about GC identifiers)

Start:
77,824,886 bp from pter
End:
77,963,474 bp from pter
Size:
138,589 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000011.8  NT_033927.7  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: SYNM_HUMAN, Q96I59 (See protein sequence)
Recommended Name: Probable asparaginyl-tRNA synthetase, mitochondrial precursor  
Size: 477 amino acids; 54077 Da
Subcellular location: Mitochondrion matrix (By similarity)

REFSEQ proteins: NP_078954.4  

ENSEMBL proteins: 
ENSP00000281038 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (NARS2)
Human Recombinant Proteins from Abnova (NARS2)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005737 cytoplasm IEA--
GO:0005739 mitochondrion ISS--
GO:0005759 mitochondrial matrix IEA--
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Antibodies for NARS2: 
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Sigma-Aldrich Antibodies for NARS2
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Antibodies from Abcam (NARS2), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (NARS2)
Novus Biologicals Antibodies for NARS2

Assays for NARS2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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5/7 InterPro domains/families (see all 7 ):
 IPR012340 NA-bd_OB-fold
 IPR004365 NA_bd_OB_tRNA-helicase
 IPR004522 Asn-tRNA-synth_IIb
 IPR006195 aa-tRNA-synth_II_cons-reg
 IPR002312 Asp-tRNA-synth_IIb


   GeneDecks  NARS2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q96I59

ProtoNet protein and cluster: Q96I59

3 Blocks protein families:
IPB002312 Aspartyl-tRNA synthetase signature
IPB004364 tRNA synthetase
IPB004365 OB-fold nucleic acid binding domain


UniProtKB/Swiss-Prot: SYNM_HUMAN, Q96I59
Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NARS2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (NARS2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_024678

              Applied Biosystems Silencer® siRNAs for NARS2

              Sigma-Aldrich siRNA for NARS2  
                     Sigma-Aldrich shRNA for NARS2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for NARS2
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_024678
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_024678
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_024678 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_024678

UniProtKB/Swiss-Prot: SYNM_HUMAN, Q96I59
Catalytic activity: ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn)
Enzyme Number (IUBMB): EC 6.1.1.22 

5/6 Gene Ontology (GO) molecular function terms (links to tree view) (see all 6 ):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0003676 nucleic acid binding IEA--
GO:0004815 aspartate-tRNA ligase activity IEA--
GO:0004816 asparagine-tRNA ligase activity ISS--
GO:0005524 ATP binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for  NARS2  (Your Favorite Gene powered by Ingenuity) 
 Aminoacyl-tRNA Biosynthesis
 Alanine and Aspartate Metabolism

   GeneDecks  NARS2 for the pathways selected above  
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2 Kegg Pathways  (Kegg details for NARS2):
 hsa00252 Alanine and aspartate metabolism
 hsa00970 Aminoacyl-tRNA biosynthesis

   GeneDecks  NARS2 for the pathways selected above  
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2 Interacting proteins for NARS2 (ENSP000002810383) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
ASNSENSP000001755063STRING (score=.899)
NARSENSP000002568543STRING (score=.843)
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2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006421 asparaginyl-tRNA aminoacylation IEA--
GO:0006422 aspartyl-tRNA aminoacylation ISS--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for NARS2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NARS2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (NARS2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_024678

              Sigma-Aldrich siRNA for NARS2  
                     Sigma-Aldrich shRNA for NARS2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_024678  

REFSEQ mRNAs for NARS2 gene: 

NM_024678.4   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_024678  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_024678
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_024678
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_024678 

Additional cDNA sequence: 

AK027094.1 AK056980.1 AK225444.1 AK315425.1 BC007800.2 CR600469.1 

8 DOTS entries:

DT.411699  DT.100766180  DT.95077558  DT.100804478  DT.92425669  DT.95077555  DT.120746611  DT.91665164 

24/143 AceView cDNA sequences (see all 143 ):

AI436094 AI654128 BI823322 BU176731 CR600469 CA419766 NM_024678 AI983291 
BQ011575 CA313358 AI183590 BQ372908 CD520140 CB151891 BC007800 BI251987 
CB157768 BP338428 AW968557 AI859305 AI025839 AI347816 BP353228 CD632206 

highest scoring ESTs for NARS2:

AA913454 AI088432 AI436094 AI524827 AI697717 AK056980 AW968557 BC007800 BF208518 BF315238 

Unigene Cluster for NARS2:

Asparaginyl-tRNA synthetase 2, mitochondrial (putative)
Hs.503389  [show with all ESTs]
Unigene Representative Sequence: AK056980


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000281038  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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NARS2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for NARS2

1 / 2 / 3

3 probe-sets matching NARS2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

60018_at2, 3 U95-C 1 1.00 1.00 1.00 1.00 AI697717 0.80 1.00 0.91 1

219217_at2, 3 U133-A 1 1.00 1.00 -- -- NM_024678 0.60 1.00 0.82 1

219217_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TTTGGATTTT

SOURCE GeneReport for Unigene cluster: Hs.503389
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for NARS2 gene from 5/22 species (see all 22 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
NARS21   -- asparaginyl-tRNA synthetase 2, mitochondrial (putative) 89.66(n)
88.26(a)
608831  XM_845968.1  XP_851061.1 
chimpanzee
(Pan troglodytes)
NARS21   -- asparaginyl-tRNA synthetase 2, mitochondrial (putative) 99.65(n)
99.37(a)
466723  XM_522122.2  XP_522122.2 
cow
(Bos taurus)
NARS21   -- asparaginyl-tRNA synthetase 2, mitochondrial (putative) 89.59(n)
87.21(a)
504824  XM_581002.3  XP_581002.2 
rat
(Rattus norvegicus)
Nars21   -- asparaginyl-tRNA synthetase 2 (mitochondrial)(putative) 83.97(n)
82.86(a)
293128  NM_001034921.1  NP_001030093.1 
mouse
(Mus musculus)
Nars21, 5 75
asparaginyl-tRNA synthetase 2 (mitochondrial)(putative)1, 5 85.26(n)1
84.49(a)1
2441411  NM_153591.31  NP_705819.21 
 AI8751995  AK0467995  (see all 16)
About this table        Species with no ortholog for NARS2

ENSEMBL Gene Tree for NARS2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for NARS2 gene
NARS2  DARS2  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
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10/777 NCBI SNPs in NARS2 are shown (see all 777 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 240)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 11 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs105014291,2
A,C,F,H77957438(+) CAAAAG/TTTAAT 1 T/N mis118Minor allele frequency- N:0.00NS EA NA EU WA 1334
rs107933381,2
A,C,F,H77964680(+) AAAGAG/TACTAC 1 -- ng31 trp38Minor allele frequency- T:0.20EU EA WA 834
rs107368141,2
A,C,F,H77964110(+) GGAAAG/CTCGAG 1 -- ng314Minor allele frequency- C:0.31EU WA MN EA 1482
rs107933371,2
A,C,F77963523(+) CTACCT/ACACCA 1 -- ng316Minor allele frequency- A:0.36MN EA NA 1614
rs124219031,2
C,F,H77964706(+) TAAGTG/ACACAC 1 -- ng317Minor allele frequency- A:0.02EU EA WA NA 562
rs107512961,2
A,C,F,H77954925(+) CGCAGG/ATACTC 1 Y/Y syn110Minor allele frequency- A:0.40EU EA WA NA 922
rs122852661,2
C,F,H77881859(+) AAGGTC/TGGACC 1 P/P syn14Minor allele frequency- T:0.02EU EA WA 420
rs20637281,2
A,C,F,H,O77863284(+) ATCCAG/CCCTAA 1 -- int16Minor allele frequency- C:0.32MN EU EA WA 782
rs107933131,2
A,C,F,H77846958(+) CTAAAC/TATGAA 1 -- int14Minor allele frequency- T:0.30EU EA WA 420
rs65927941,2
A,C,F,H77953391(+) TTTACG/AATTGT 1 -- int14Minor allele frequency- A:0.28EU EA WA 378
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HapMap Linkage Disequilibrium images for NARS2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
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OMIM: 612803

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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8 PubMed articles for NARS2 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 79731 HGNC: 26274 AceView: FLJ23441 Ensembl:ENSG00000137513 euGenes: HUgn79731
ECgene: NARS2 H-InvDB: NARS2
(According to HUGE)
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  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for NARS2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Predesigned and custom siRNAs for NARS2 Antibodies for NARS2
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for NARS2 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

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(NARS2)
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 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for NARS2

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