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NARS Gene

protein-coding   GIFtS: 63

GC18M053418
asparaginyl-tRNA synthetase
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
ASNRS 2, 5
ASNS 3
AsnRS 3
EC 6.1.1.22 3
NARS1 1, 2
Descriptions
Asparagine--tRNA ligase 3
asparagine tRNA ligase 1, cytoplasmic 2
asparaginyl-tRNA synthetase 2
External Ids
HGNC: 76431
Entrez Gene: 46772
UniProtKB: O437763
Ensembl: ENSG000001344407
Search outside databases for aliases for NARS gene

Previous GC identifers: GC18M055658 GC18M055242 GC18M055052 GC18M053416

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for NARS:
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids.
Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of
tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic
asparaginyl-tRNA synthetases. [provided by RefSeq]

Gene Wiki entry for NARS
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NARS gene  

Entrez Gene cytogenetic band: 18q21.2-q21.3   Ensembl cytogenetic band:  18q21.31   HGNC cytogenetic band: 18q21.31

NARS Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 18         GeneLoc Exon Structure

GeneLoc location for GC18M053418:     (about GC identifiers)

Start:
53,418,889 bp from pter
End:
53,440,175 bp from pter
Size:
21,287 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000018.8  NT_025028.13  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: SYNC_HUMAN, O43776 (See protein sequence)
Recommended Name: Asparaginyl-tRNA synthetase, cytoplasmic  
Size: 548 amino acids; 62943 Da
Subcellular location: Cytoplasm
Secondary accessions: Q53GU6

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_004530.1  

    ENSEMBL proteins: 
    ENSP00000256854 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (NARS)
    Human Recombinant Proteins from Abnova (NARS)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005625 soluble fraction TAS9421509
    GO:0005737 cytoplasm TAS9421509
    About this table

    Antibodies for NARS: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for NARS
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (NARS), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (NARS)
    Novus Biologicals Antibodies for NARS

    Assays for NARS: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/7 InterPro domains/families (see all 7 ):
     IPR012340 NA-bd_OB-fold
     IPR004365 NA_bd_OB_tRNA-helicase
     IPR004522 Asn-tRNA-synth_IIb
     IPR006195 aa-tRNA-synth_II_cons-reg
     IPR002312 Asp-tRNA-synth_IIb


       GeneDecks  NARS for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O43776

    ProtoNet protein and cluster: O43776

    3 Blocks protein families:
    IPB002312 Aspartyl-tRNA synthetase signature
    IPB004364 tRNA synthetase
    IPB004365 OB-fold nucleic acid binding domain


    UniProtKB/Swiss-Prot: SYNC_HUMAN, O43776
    Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NARS)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NARS)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004539

                  Applied Biosystems Silencer® siRNAs for NARS

                  Sigma-Aldrich siRNA for NARS  
                         Sigma-Aldrich shRNA for NARS  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for NARS
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_004539
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_004539
                                     untagged cDNA clone in CMV expression vector: NM_004539 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_004539

    UniProtKB/Swiss-Prot: SYNC_HUMAN, O43776
    Catalytic activity: ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn)
    Enzyme Number (IUBMB): EC 6.1.1.22 

    Genatlas biochemistry entry for NARS:
    asparaginyl-tRNA synthetase

    5/7 Gene Ontology (GO) molecular function terms (links to tree view) (see all 7 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0003676 nucleic acid binding IEA--
    GO:0004815 aspartate-tRNA ligase activity IEA--
    GO:0004816 asparagine-tRNA ligase activity TAS9421509
    GO:0005515 protein binding IPI15231747
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    2 Sigma-Aldrich "Your Favorite Gene" Pathways for  NARS  (Your Favorite Gene powered by Ingenuity) 
     Alanine and Aspartate Metabolism
     Aminoacyl-tRNA Biosynthesis

       GeneDecks  NARS for the pathways selected above  
    About GeneDecksing

    2 Kegg Pathways  (Kegg details for NARS):
     hsa00252 Alanine and aspartate metabolism
     hsa00970 Aminoacyl-tRNA biosynthesis

       GeneDecks  NARS for the pathways selected above  
    About GeneDecksing

    5/10 Interacting proteins for NARS (O437761, 2 ENSP000002568543) via UniProtKB, MINT, and/or STRING (see all 10 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    LSM1O151161EBI-373505, EBI-347619
    ATXN1P542532MINT-2861028
    ASNSENSP000001755063STRING (score=.978)
    NARS2ENSP000002810383STRING (score=.843)
    AARSENSP000002617723STRING (score=.807)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006421 asparaginyl-tRNA aminoacylation IEA--
    GO:0006422 aspartyl-tRNA aminoacylation IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for NARS
    4 Novoseek chemical compound relationships for NARS gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    aminoacyl-trna 75.42 1 18285520 (1)
    trna 73.98 31 9421509 (3), 10993083 (2), 16800632 (2), 17393393 (2) (see all 7)
    asparagine 53.01 4 8636057 (2), 16408313 (1)
    uranium 49.69 2 10976874 (1), 16609186 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NARS)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NARS)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004539

                  Sigma-Aldrich siRNA for NARS  
                         Sigma-Aldrich shRNA for NARS  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_004539  

    REFSEQ mRNAs for NARS gene: 

    NM_004539.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_004539  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_004539
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_004539
                                     untagged cDNA clone in CMV expression vector: NM_004539 

    Additional cDNA sequence: 

    AJ000334.1 AK129897.1 AK222835.1 AK293985.1 AK294364.1 AK294763.1 AK297777.1 BC001687.1 
    CR609633.1 D84273.1 U79254.1 

    24/28 DOTS entries (see all 28 ):

    DT.419312  DT.97855359  DT.102832966  DT.121084213  DT.100869391  DT.100673910  DT.100848843  DT.91722905 
    DT.100673905  DT.95083245  DT.422320  DT.91769794  DT.92023625  DT.100673909  DT.100673906  DT.92404386 
    DT.121084198  DT.121084209  DT.91769789  DT.95162534  DT.95162574  DT.121084181  DT.121084196  DT.121084218 

    24/637 AceView cDNA sequences (see all 637 ):

    BX956359 BX952354 AW117736 BU527745 CR609633 CK299384 BX343744 BG260345 
    BP365666 CD654817 BU665242 BQ051329 AA305871 AW150744 AA448652 BU624871 
    N93735 BQ447627 BM782185 AW204862 AW027895 BQ878169 BM839702 BQ213524 

    highest scoring ESTs for NARS:

    D84273 AA219340 AA307724 AA314303 AA361411 AA545788 AJ000334 AK129897 AL602338 AL602587 

    Unigene Cluster for NARS:

    Asparaginyl-tRNA synthetase
    Hs.465224  [show with all ESTs]
    Unigene Representative Sequence: NM_004539


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for NARS

    ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14a · 14b
    SP1:                    -                                                                                       
    SP2:                    -                                                                                       
    SP3:                                                                                                            
    SP4:                                                                                                            

    About this scheme

    ECgene alternative splicing isoforms for NARS

    1 Ensembl transcript including schematic representation:
    ENST00000256854  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    NARS expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for NARS

    1 / 2 / 3

    3 probe-sets matching NARS gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    41241_at2, 3 U95-A 2 1.00 0.97 1.00 1.00 D84273 1.00 1.00 1.00 1

    200027_at2, 3 U133-B 1 1.00 1.00 -- -- NM_004539 0.60 1.00 0.82 1

    200027_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TATTCTCAAT

    SOURCE GeneReport for Unigene cluster: Hs.465224

    Expression variation in blood from EXPOLDB for NARS

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for NARS gene from 5/17 species (see all 17 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    NARS1   -- asparaginyl-tRNA synthetase 90.94(n)
    95.26(a)
    607631  XM_843967.1  XP_849060.1 
    chimpanzee
    (Pan troglodytes)
    NARS1   -- asparaginyl-tRNA synthetase 99.09(n)
    99.45(a)
    455438  XM_001139185.1  XP_001139185.1 
    cow
    (Bos taurus)
    NARS1   -- asparaginyl-tRNA synthetase 87.65(n)
    93.43(a)
    616033  NM_001046572.1  NP_001040037.1 
    rat
    (Rattus norvegicus)
    Nars1   -- asparaginyl-tRNA synthetase 86.55(n)
    92.57(a)
    291556  NM_001025635.1  NP_001020806.1 
    mouse
    (Mus musculus)
    Nars1, 5 185
    asparaginyl-tRNA synthetase1, 5 86.35(n)1
    92.87(a)1
    702231  NM_027350.21  NP_081626.11 
     AA9601285  AK0138805  (see all 22)
    About this table        Species with no ortholog for NARS

    ENSEMBL Gene Tree for NARS
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for NARS gene
    NARS22  DARS2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/203 NCBI SNPs in NARS are shown (see all 203 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 69)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 18 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs20329381,2
    A,C,F,H53440074(-) CGCAGG/ACGCCC 1 -- ut517Minor allele frequency- A:0.17EU EA WA NA 556
    rs113561,2
    A,C,F,H53419484(-) TACCTT/GGAATT 1 -- ut31 ese39Minor allele frequency- G:0.49MN NA EU EA WA 932
    rs20329371,2
    A,C,F,H53440178(-) GTCTCT/CGGATC 1 -- ng3111Minor allele frequency- C:0.19EU EA WA NA 972
    rs20701091,2
    C,F,H53419864(-) GAAGCG/ATGGAG 1 -- ut315Minor allele frequency- A:0.22MN EU EA WA 602
    --
    rs3178321,2
    C53418551(-) agaccA/Gaggcg 1 -- ng510--------
    --
    rs175261051,2
    F53440006(-) GAGTCA/GTAAGA 1 -- ut51 ese32Minor allele frequency- G:0.03EA MN 400
    --
    rs4093051,2
    C53441131(-) CAGGGC/GCCGGA 1 -- ng310--------
    --
    rs17880001,2
    A53441922(-) tgccaA/Ccacgc 1 -- ng310--------
    rs350574871,2
    F53420711(+) AACACG/ATCGAC 1 D/D syn11Minor allele frequency- A:0.03NA 74
    rs3178301,2
    A,C,F,H,O53421934(-) ATTTCA/CCTTCT 1 -- int111Minor allele frequency- C:0.45EA NA EU 2296
    About this table

    HapMap Linkage Disequilibrium images for NARS (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 108410

    2 Novoseek disease relationships for NARS gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    myopathies idiopathic inflammatory 71.15 2 16085368 (1)
    myositis 63.37 1 12235211 (1)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/31 PubMed articles for NARS gene (see all 31 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 4677 HGNC: 7643 AceView: NARS Ensembl:ENSG00000134440 euGenes: HUgn4677
    ECgene: NARS H-InvDB: NARS
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for NARS:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for NARS-related products

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     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for NARS Antibodies for NARS
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for NARS Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant & Natural Proteins  

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     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for NARS

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