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NARF Gene

protein-coding   GIFtS: 53

GC17P078009
nuclear prelamin A recognition factor
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
DKFZp434G0420 1, 2
FLJ10067 1, 2
IOP2 1, 2, 3
Descriptions
Iron-only hydrogenase-like protein 2 3
nuclear prelamin A recognition factor 2
prenyl-dependent prelamin A binding protein 2
External Ids
HGNC: 299161
Entrez Gene: 265022
UniProtKB: Q9UHQ13
Ensembl: ENSG000001415627
Search outside databases for aliases for NARF gene

Previous GC identifers: GC17P079555 GC17P083333 GC17P080945 GC17P081095

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for NARF:
Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends.
Prenylation was initially believed to be important only for membrane attachment. However, another
role for prenylation appears to be its importance in protein-protein interactions. The only
nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins.
Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal
CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it
is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to
the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A
endoprotease complex. The encoded protein is located in the nucleus, where it partially
colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only
bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have
been identified for this gene, including one with a novel exon that is generated by RNA editing.
[provided by RefSeq]

Gene Wiki entry for NARF
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NARF gene  

Entrez Gene cytogenetic band: 17q25.3   Ensembl cytogenetic band:  17q25.3   HGNC cytogenetic band: 17q25.3

NARF Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17P078009:     (about GC identifiers)

Start:
78,009,349 bp from pter
End:
78,039,432 bp from pter
Size:
30,084 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000017.9  NT_010663.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: NARF_HUMAN, Q9UHQ1 (See protein sequence)
Recommended Name: Nuclear prelamin A recognition factor  
Size: 456 amino acids; 51156 Da
Subunit: Interacts with LMNA and binds to the farnesylated C-terminal domain
Subcellular location: Nucleus
Secondary accessions: A6NCJ3 Q96AY9 Q9BWC6
Alternative splicing: 3 isoforms:  Q9UHQ1-1   Q9UHQ1-2   Q9UHQ1-3   

Post-translational modifications:

  • Phosphorylated upon DNA damage, probably by ATM or ATR1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (4 alternative transcripts): 
    NP_001033707.1  NP_001077077.1  NP_036468.1  NP_114174.1  


    ENSEMBL proteins: 
    ENSP00000309899 ENSP00000363739 ENSP00000283996 ENSP00000374656 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (NARF)
    Human Recombinant Proteins from Abnova (NARF)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    GO:0005638 lamin filament IDA10514485
    GO:0031981 nuclear lumen IDA10514485
    About this table

    Antibodies for NARF: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for NARF
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (NARF), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (NARF)
    Novus Biologicals Antibodies for NARF

    Assays for NARF: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR004108 Fe_hydrogenase_lsu_C
     IPR003149 Fe_hydrogenase_ssu-like


       GeneDecks  NARF for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9UHQ1

    ProtoNet protein and cluster: Q9UHQ1

    UniProtKB/Swiss-Prot: NARF_HUMAN, Q9UHQ1
    Similarity: Belongs to the NARF family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NARF)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NARF)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_012336

                  Applied Biosystems Silencer® siRNAs for NARF

                  Sigma-Aldrich siRNA for NARF  
                         Sigma-Aldrich shRNA for NARF  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for NARF
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 4): NM_001038618
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 4): NM_001038618
                                     untagged cDNA clones in CMV expression vector (see all 4): NM_001038618 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_031968

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005521 lamin binding IPI10514485
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    1 Interacting protein for NARF (ENSP000003637393) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    LMNAENSP000003572833STRING (score=.742)
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for NARF

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NARF)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NARF)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_012336

                  Sigma-Aldrich siRNA for NARF  
                         Sigma-Aldrich shRNA for NARF  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001038618  NM_001083608  NM_012336  NM_031968  

    REFSEQ mRNAs for NARF gene (4 alternative transcripts): 

    NM_001038618.2   NM_001083608.1   NM_012336.3   NM_031968.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001038618  NM_001083608  NM_012336  NM_031968  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 4): NM_001038618
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 4): NM_001038618
                                     untagged cDNA clones in CMV expression vector (see all 4): NM_001038618 

    Additional cDNA sequence: 

    AF128406.1 AK000929.1 AK056966.1 AK093717.1 AK297875.1 AK303155.1 AK307351.1 AL137729.1 
    BC000438.2 BC016440.1 CR590238.1 CR595670.1 CR595982.1 CR597828.1 CR598397.1 CR601772.1 
    CR603660.1 CR603694.1 CR606462.1 CR611448.1 CR612983.1 CR619657.1 CR620704.1 

    24/27 DOTS entries (see all 27 ):

    DT.100890151  DT.100890145  DT.100890169  DT.416841  DT.95364511  DT.100890160  DT.100890150  DT.100890164 
    DT.100890156  DT.91729281  DT.120948879  DT.120948878  DT.100676289  DT.100890170  DT.120948942  DT.40269535 
    DT.40316311  DT.95364496  DT.100890147  DT.102832639  DT.120811836  DT.120948901  DT.120948932  DT.91653691 

    24/371 AceView cDNA sequences (see all 371 ):

    BQ574644 CR611448 BQ649482 AI338665 BI906906 CR595670 CR603694 BM977606 
    AI886089 BU734059 CB131418 BU656272 AW969088 AL534171 BQ951167 CR598397 
    AI085650 BI770351 BX442215 AU280807 BG389476 BG397223 BE349169 BM796251 

    highest scoring ESTs for NARF:

    AI963118 AA487570 AA488695 AA504341 AA515401 AA554784 AA568721 AA584420 AA602553 AA603444 

    Unigene Cluster for NARF:

    Nuclear prelamin A recognition factor
    Hs.256526  [show with all ESTs]
    Unigene Representative Sequence: CR620704


    GeneLoc Exon Structure

    5/11 Alternative Splicing Database (ASD) splice patterns (SP) for NARF (see all 11 )

    ExUns: 1a · 1b ^ 2a · 2b · 2c ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6a · 6b · 6c ^ 7 ^ 8a · 8b ^ 9a · 9b ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14
    SP1:                                -           -                                               -     -                       -                     
    SP2:        -     -     -     -     -           -                                               -     -           -           -                     
    SP3:                                -           -                                               -     -           -           -                     
    SP4:                                -           -     -     -                                   -     -           -           -                     
    SP5:                                                                                            -     -           -                                 

    About this scheme

    ECgene alternative splicing isoforms for NARF

    4 Ensembl transcripts including schematic representations:
    ENST00000309794  ENST00000374611  ENST00000345415  ENST00000390006  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    NARF expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for NARF

    1 / 2 / 3

    4 probe-sets matching NARF gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    91771_at2, 3 U95-D 1 1.00 1.00 0.92 1.03 AL042850 0.60 1.00 0.82 1
    56342_at2, 3 U95-B 2 1.00 0.97 0.89 0.98 AI963118 1.00 1.00 1.00 1

    219862_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_012336 0.60 1.00 0.82 1

    219862_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  NARF for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GGCTGACTAA

    SOURCE GeneReport for Unigene cluster: Hs.256526

    UniProtKB/Swiss-Prot: NARF_HUMAN, Q9UHQ1
    Tissue specificity: Ubiquitous. Predominantly expressed in skeletal muscle, heart and brain

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for NARF gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    NARF1   -- nuclear prelamin A recognition factor 83.63(n)
    84.15(a)
    480819  XM_537935.2  XP_537935.2 
    chimpanzee
    (Pan troglodytes)
    NARF1   -- nuclear prelamin A recognition factor 92.46(n)
    90.95(a)
    454995  XM_511766.2  XP_511766.2 
    cow
    (Bos taurus)
    NARF1   -- nuclear prelamin A recognition factor 85.27(n)
    83.71(a)
    618340  XM_870671.2  XP_875764.2 
    rat
    (Rattus norvegicus)
    Narf1   -- nuclear prelamin A recognition factor 82.75(n)
    86.4(a)
    360681  NM_001039207.1  NP_001034296.1 
    mouse
    (Mus musculus)
    Narf1, 5 115
    nuclear prelamin A recognition factor1, 5 82.89(n)1
    85.31(a)1
    676081  NM_026272.21  NP_080548.21 
     AA9812705  AK0038555  (see all 13)
    About this table        Species with no ortholog for NARF

    ENSEMBL Gene Tree for NARF
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for NARF gene
    NARFL2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/192 NCBI SNPs in NARF are shown (see all 192 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 58)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 17 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs72097881,2
    A,C,F,H78009203(+) TTTGTG/AAGCTG 4 -- ng5112Minor allele frequency- A:0.33MN EA EU WA NA 2270
    rs72185341,2
    A,C,F,H78009270(+) TCAAAC/TGGCCA 4 -- ng516Minor allele frequency- T:0.29MN EA EU WA 1664
    rs65021131,2
    A,C,H78008604(+) atacaC/Taaaca 4 -- ng514Minor allele frequency- T:0.00EU EA WA 406
    --
    rs115379101,2
    C,H78036071(-) AACAGC/GTCCTT 4 E/D mis18Minor allele frequency- G:0.00EU EA WA 808
    rs47896901,2
    C,H78008238(+) atttgA/Ggtagt 4 -- ng51 tfbs34Minor allele frequency- G:0.00EU EA WA 384
    --
    rs129414901,2
    A,F78009358(+) TCCGCT/CGAGCC 4 -- ng51 ut512Minor allele frequency- C:0.26MN EA 1252
    --
    rs283659431,2
    F78009379(+) GACGWG/CAAGTG 4 -- ut51 ng51 tfbs32Minor allele frequency- C:0.26MN EA 1252
    --
    rs114904731,2
    A78008192(+) AAAAAA/CAAAAA 4 -- ng510--------
    --
    rs47896911,2
    C78008290(+) tttccC/Tggcct 4 -- ng51 trp30--------
    --
    rs81822881,2
    C78008184(+) aaaaaA/Caaaaa 4 -- ng510--------
    About this table

    HapMap Linkage Disequilibrium images for NARF (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 605349

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/13 PubMed articles for NARF gene (see all 13 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 26502 HGNC: 29916 AceView: NARF Ensembl:ENSG00000141562 euGenes: HUgn26502
    ECgene: NARF H-InvDB: NARF
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for NARF:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Predesigned and custom siRNAs for NARF Antibodies for NARF
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for NARF Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Antibodies for NARF

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