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NAPSA Gene

protein-coding   GIFtS: 53

GC19M055555
napsin A aspartic peptidase
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
ASP4 3
EC 3.4.23.- 3
KAP 1, 2
Kdap 1, 2
NAP1 1, 2, 3
NAPA 1, 2, 3
Napsin-1 3
SNAPA 2
TA01/TA02 3
Descriptions
Asp 4 3
Aspartyl protease 4 3
napsin A 2
napsin A aspartic peptidase 2
pronapsin A 2
External Ids
HGNC: 133951
Entrez Gene: 94762
UniProtKB: O960093
Ensembl: ENSG000001314007
Search outside databases for aliases for NAPSA gene

Previous GC identifer: GC19M055554

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for NAPSA:
The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These
peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control
of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly
in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated
aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its
C-terminus. [provided by RefSeq]

UniProtKB/Swiss-Prot: NAPSA_HUMAN, O96009
Function: May be involved in processing of pneumocyte surfactant precursors

Gene Wiki entry for NAPSA

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NAPSA gene  

Entrez Gene cytogenetic band: 19q13.33   Ensembl cytogenetic band:  19q13.33   HGNC cytogenetic band: 19q13.33

NAPSA Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19M055555:     (about GC identifiers)

Start:
55,553,546 bp from pter
End:
55,560,743 bp from pter
Size:
7,198 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000019.8  NT_011109.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: NAPSA_HUMAN, O96009 (See protein sequence)
Recommended Name: Napsin-A precursor  
Size: 420 amino acids; 45387 Da
Secondary accessions: Q8WWD9

REFSEQ proteins: NP_004842.1  

ENSEMBL proteins: 
ENSP00000253719 


Human Recombinant Proteins 
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Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (NAPSIN A)
Human Recombinant Proteins from Abnova (NAPSA)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 


Antibodies for NAPSA: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for NAPSA
Browse R&D Systems for Antibodies
Antibodies from Abcam (NAPSIN A), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (NAPSA)
Novus Biologicals Antibodies for NAPSA

Assays for NAPSA: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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3 InterPro domains/families:
 IPR009007 Peptidase_aspartic_catalytic
 IPR001461 Peptidase_A1
 IPR001969 Peptidase_aspartic_AS


   GeneDecks  NAPSA for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry O96009

ProtoNet protein and cluster: O96009

1 Blocks protein family: IPB001461 Pepsin (A1) aspartic protease family signature

UniProtKB/Swiss-Prot: NAPSA_HUMAN, O96009
Similarity: Belongs to the peptidase A1 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NAPSA)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004851

              Applied Biosystems Silencer® siRNAs for NAPSA

              Sigma-Aldrich siRNA and siRNA Panels for NAPSA  
                     Sigma-Aldrich shRNA for NAPSA  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for NAPSA
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_004851
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_004851
                                 untagged cDNA clone in CMV expression vector: NM_004851 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_004851

UniProtKB/Swiss-Prot: NAPSA_HUMAN, O96009
Function: May be involved in processing of pneumocyte surfactant precursors
Enzyme Number (IUBMB): EC 3.4.23.- 

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004190 aspartic-type endopeptidase activity TAS9877162
GO:0008233 peptidase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section



3 Interacting proteins for NAPSA (O960092 ENSP000002537193) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
YWHAZP631042MINT-3318098
ELF5ENSP000003110103STRING (score=.916)
KRTDAPENSP000003392513STRING (score=.744)
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1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006508 proteolysis TAS9877162
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for NAPSA

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NAPSA)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004851

              Sigma-Aldrich siRNA and siRNA Panels for NAPSA  
                     Sigma-Aldrich shRNA for NAPSA  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_004851  

REFSEQ mRNAs for NAPSA gene: 

NM_004851.1   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_004851  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_004851
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_004851
                                 untagged cDNA clone in CMV expression vector: NM_004851 

Additional cDNA sequence: 

AF090386.1 AF098484.1 AF200345.1 AK308013.1 AK314364.1 BC017842.1 BC029397.1 CR619860.1 

9 DOTS entries:

DT.100023953  DT.97846383  DT.115036  DT.95244686  DT.40206656  DT.91642990  DT.95244706  DT.121420516 
DT.95244712 

24/215 AceView cDNA sequences (see all 215 ):

AI734145 BM996247 BU541597 AW139459 BM972069 BQ712631 AI005080 BI823670 
AW183525 BQ058202 BU688522 AF090386 AF098484 BP340501 BM992609 BQ055704 
AA279744 BM921728 BQ019267 BC006149 BC040958 BC053864 AF200345 BG540257 

highest scoring ESTs for NAPSA:

AA359075 AF090386 AF098484 AF200345 AW025398 BC017842 BF869544 BG537203 BG540257 BG541671 

Unigene Cluster for NAPSA:

Napsin A aspartic peptidase
Hs.714418  [show with all ESTs]
Unigene Representative Sequence: AF098484


GeneLoc Exon Structure

5/6 Alternative Splicing Database (ASD) splice patterns (SP) for NAPSA (see all 6 )

ExUns: 1a · 1b ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9a · 9b · 9c
SP1:              -     -                 -                                                   
SP2:                                -     -                                                   
SP3:              -     -           -     -                                                   
SP4:                                      -     -                                             
SP5:                                                                                          

About this scheme

ECgene alternative splicing isoforms for NAPSA

1 Ensembl transcript including schematic representation:
ENST00000253719  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

NAPSA expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for NAPSA

1 / 2 / 3

3 probe-sets matching NAPSA gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

90836_at2, 3 U95-E 1 1.00 1.00 1.00 1.00 AW025398 0.80 0.88 0.84 1

223806_s_at2, 3 U133-B 1 1.00 1.00 -- -- AF090386 0.80 0.88 0.84 1

223806_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: CGCAGCGGGT

SOURCE GeneReport for Unigene cluster: Hs.714418

UniProtKB/Swiss-Prot: NAPSA_HUMAN, O96009
Tissue specificity: Expressed predominantly in adult lung (type II pneumocytes) and kidney and in
fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for NAPSA gene from 5/6 species (see all 6 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
NAPSA1   -- napsin A aspartic peptidase 84.14(n)
79.37(a)
476406  XM_533610.2  XP_533610.2 
chimpanzee
(Pan troglodytes)
NAPSA1   -- napsin A aspartic peptidase 99.29(n)
98.57(a)
468961  XM_524345.2  XP_524345.2 
cow
(Bos taurus)
NAPSA1   -- napsin A aspartic peptidase 84.61(n)
79.75(a)
539259  XM_585968.3  XP_585968.2 
rat
(Rattus norvegicus)
Napsa1   -- napsin A aspartic peptidase 78.69(n)
72.15(a)
60575  NM_031670.1  NP_113858.1 
mouse
(Mus musculus)
Napsa1, 5 75
napsin A aspartic peptidase1, 5 77.89(n)1
71.95(a)1
165411  NM_008437.11  NP_032463.11 
 AB0381445  AJ2507185  (see all 12)
About this table        Species with no ortholog for NAPSA

ENSEMBL Gene Tree for NAPSA
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for NAPSA gene
NAPSB2  BACE12  CTSD2  BACE22  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/58 NCBI SNPs in NAPSA are shown (see all 58 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 22)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 19 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs6763141,2
A,C,F,H,O55557347(-) CAGGAC/TCCTGA 1 I/T mis1 ese314Minor allele frequency- A:0.02MN NA EU EA WA 1324
rs8738341,2
A,C,F,H55560814(-) GTCACC/TTGCGG 1 -- ng318Minor allele frequency- T:0.03EU EA WA NA 568
--
rs96768441,2
C55562651(+) tctctC/Gtctct 1 -- ng310--------
--
rs72528041,2
A55562066(+) tttttA/Gtattt 1 -- ng310--------
--
rs48027001,2
C55562616(+) ctctcC/Tccctc 1 -- ng31 trp30--------
--
rs104132281,2
C55561885(+) gcaccC/Ggatac 1 -- ng310--------
--
rs48027011,2
C55562618(+) ctctcC/Tctctc 1 -- ng31 trp30--------
--
rs6382941,2
A,C,F55554835(-) GTCCCT/CGCCTA 1 P/P syn110Minor allele frequency- C:0.24EA NA MN 2044
--
rs5991001,2
A,C,F,H55559541(+) tagccG/Aggcgt 1 -- int13Minor allele frequency- A:0.26EU EA 294
rs12745171,2
C,F,H,O55560566(-) GGATTT/CTCTGC 1 -- int114Minor allele frequency- C:0.25EU EA WA NA 2636
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HapMap Linkage Disequilibrium images for NAPSA (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 605631

6 Novoseek disease relationships for NAPSA gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
adenocarcinoma lung 73.22 12 16259111 (6), 11050472 (1), 15221555 (1), 10580105 (1) (see all 6)
lung carcinoma 67.00 3 16259111 (2)
adenocarcinoma 49.02 14 16259111 (4), 17636482 (2), 15832091 (1), 19438742 (1) (see all 6)
cancer lung 27.27 1 12151090 (1)
metastasis 27.11 1 17636482 (1)
tumors 7.41 4 15221555 (1), 15754301 (1), 17636482 (1), 18195689 (1)
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(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/35 PubMed articles for NAPSA gene (see all 35 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 9476 HGNC: 13395 AceView: NAPSAandNAPSB Ensembl:ENSG00000131400 euGenes: HUgn9476
ECgene: NAPSA H-InvDB: NAPSA
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for NAPSA:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Predesigned and custom siRNAs for NAPSA Antibodies for NAPSA
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for NAPSA Browse small molecules at Sigma-Aldrich
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 Antibodies for NAPSA

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