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NAPRT1 Gene

protein-coding   GIFtS: 51

GC08M144730
nicotinate phosphoribosyltransferase domain containing 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
EC 2.4.2.11 3
FHIP 3
NAPRTase 3
PP3856 1, 2
Descriptions
FHA-HIT-interacting protein 3
Nicotinate phosphoribosyltransferase domain-containing
protein 1 3
nicotinate phosphoribosyltransferase domain containing 1 2
nicotinic acid phosphoribosyltransferase 2
External Ids
HGNC: 304501
Entrez Gene: 931002
UniProtKB: Q6XQN63
Ensembl: ENSG000001478137
Search outside databases for aliases for NAPRT1 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for NAPRT1:
Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC
2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD),
and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular
redox reactions and is an essential component of a variety of processes in cellular metabolism
including response to stress (Hara et al., 2007).[supplied by OMIM]

UniProtKB/Swiss-Prot: PNCB_HUMAN, Q6XQN6
Function: Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). Essential
for NA to increase cellular NAD levels and prevent oxidative stress of the cells

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NAPRT1 gene  

Entrez Gene cytogenetic band: 8q24.3   Ensembl cytogenetic band:  8q24.3   HGNC cytogenetic band: 8q24.3

NAPRT1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08M144730:     (about GC identifiers)

Start:
144,728,098 bp from pter
End:
144,731,651 bp from pter
Size:
3,554 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000008.9  NT_023684.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: PNCB_HUMAN, Q6XQN6 (See protein sequence)
Recommended Name: Nicotinate phosphoribosyltransferase  
Size: 538 amino acids; 57578 Da
Subcellular location: Cytoplasm, cytosol
Secondary accessions: A7BFI3 Q6PJL1 Q6XQN4 Q6XQN5 Q8N5E8 Q9BRG0
Alternative splicing: 4 isoforms:  Q6XQN6-1   Q6XQN6-2   Q6XQN6-3   Q6XQN6-4   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_660202.3  

    ENSEMBL proteins: 
    ENSP00000341136 ENSP00000276844 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (NAPRT1)
    Human Recombinant Proteins from Abnova (NAPRT1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm IEA--
    GO:0005829 cytosol IDA17604275
    About this table

    Antibodies for NAPRT1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for NAPRT1
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (NAPRT1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (NAPRT1)
    Novus Biologicals Antibodies for NAPRT1

    Assays for NAPRT1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR006405 Nic_PRibTrfase_put
     IPR015977 Nic_PRibTrfase-like


       GeneDecks  NAPRT1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q6XQN6

    ProtoNet protein and cluster: Q6XQN6

    1 Blocks protein family: IPB007229 Nicotinate phosphoribosyltransferase and related

    UniProtKB/Swiss-Prot: PNCB_HUMAN, Q6XQN6
    Similarity: Belongs to the NAPRTase family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NAPRT1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_145201

                  Applied Biosystems Silencer® siRNAs for NAPRT1

                  Sigma-Aldrich siRNA for NAPRT1  
                         Sigma-Aldrich shRNA for NAPRT1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for NAPRT1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_145201
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_145201
                                     untagged cDNA clones in CMV expression vector (see all 2): AK097002 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_145201

    UniProtKB/Swiss-Prot: PNCB_HUMAN, Q6XQN6
    Function: Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). Essential
    for NA to increase cellular NAD levels and prevent oxidative stress of the cells
    Catalytic activity: Nicotinate D-ribonucleotide + diphosphate = nicotinate +
    5-phospho-alpha-D-ribose 1-diphosphate
    Enzyme Number (IUBMB): EC 2.4.2.11 

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004516 nicotinate phosphoribosyltransferase activity IDA17604275
    GO:0016757 transferase activity, transferring glycosyl groups IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  NAPRT1  (Your Favorite Gene powered by Ingenuity) 
     Nicotinate and Nicotinamide Metabolism

       GeneDecks  NAPRT1 for the pathways selected above  
    About GeneDecksing

    UniProtKB/Swiss-Prot: PNCB_HUMAN, Q6XQN6
    Pathway: Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate ribonucleotide from nicotinate:
    step 1/1


    2 Interacting proteins for NAPRT1 (ENSP000002768443) via UniProtKB, MINT, and/or STRING

    InteractantInteraction Details
    GeneCardExternal ID(s)
    NAMPTENSP000002225533STRING (score=.917)
    QPRTENSP000002197713STRING (score=.736)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006979 response to oxidative stress IMP17604275
    GO:0019363 pyridine nucleotide biosynthetic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for NAPRT1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NAPRT1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_145201

                  Sigma-Aldrich siRNA for NAPRT1  
                         Sigma-Aldrich shRNA for NAPRT1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_145201  

    REFSEQ mRNAs for NAPRT1 gene: 

    NM_145201.4   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_145201  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_145201
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_145201
                                     untagged cDNA clones in CMV expression vector (see all 2): AK097002 

    Additional cDNA sequence: 

    AB242230.1 AF258565.1 AK097002.1 AY214325.1 AY214326.1 AY214327.1 BC006284.2 BC032466.1 
    CR591708.1 CR592925.1 

    14 DOTS entries:

    DT.95293101  DT.97813675  DT.75101665  DT.100751570  DT.100682281  DT.75101664  DT.120634731  DT.412214 
    DT.95120888  DT.100751572  DT.120634822  DT.92058858  DT.95213902  DT.97813644 

    22 AceView cDNA sequences:

    AW806799 BM826760 CA407797 AW806800 AI458775 CR591708 AW806801 AI476528 
    CR592925 BM818808 AW751138 BC006284 AW516824 AI871064 BG746945 CD671444 
    BM768570 AW806779 BM818957 BG824256 BG746868 NM_145201 

    highest scoring ESTs for NAPRT1:

    AI871064 AW516824 BC006284 BG746868 BG746945 BG824256 AA621834 AA649311 AI185086 AI431636 

    Unigene Cluster for NAPRT1:

    Nicotinate phosphoribosyltransferase domain containing 1
    Hs.493164  [show with all ESTs]
    Unigene Representative Sequence: AY214325


    GeneLoc Exon Structure

    5/10 Alternative Splicing Database (ASD) splice patterns (SP) for NAPRT1 (see all 10 )

    ExUns: 1 ^ 2a · 2b ^ 3a · 3b · 3c ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7a · 7b · 7c ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^ 14a · 14b
    SP1:                    -                                         -                                                     -                     
    SP2:                    -                                         -                                                                           
    SP3:                    -                                         -                                                                           
    SP4:                    -                                         -                                                                           
    SP5:                                                                                                                                          

    About this scheme

    ECgene alternative splicing isoforms for NAPRT1

    2 Ensembl transcripts including schematic representations:
    ENST00000340490  ENST00000276844  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    NAPRT1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for NAPRT1

    1 / 2 / 3

    4 probe-sets matching NAPRT1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    63030_at2, 3 U95-C 1 1.00 1.00 0.96 1.01 AI925574 0.60 1.00 0.82 1

    89340_at2, 3 U95-E 1 0.56 1.00 0.92 0.99 AL042450 0.60 1.00 0.82 1

    226707_at2, 3 U133-B 1 1.00 1.00 -- -- AI925574 0.60 1.00 0.82 1

    226707_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CTGCAGTTAG

    SOURCE GeneReport for Unigene cluster: Hs.493164
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for NAPRT1 gene from 5/6 species (see all 6 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    NAPRT11   -- nicotinate phosphoribosyltransferase domain containing more 84.11(n)
    87.67(a)
    609412  XM_846661.1  XP_851754.1 
    cow
    (Bos taurus)
    NAPRT11   -- nicotinate phosphoribosyltransferase domain containing more 86.74(n)
    89.57(a)
    399559  XM_594775.3  XP_594775.3 
    rat
    (Rattus norvegicus)
    Naprt11   -- nicotinate phosphoribosyltransferase domain containing more 81.65(n)
    83.66(a)
    315085  NM_207609.1  NP_997492.1 
    mouse
    (Mus musculus)
    Naprt11, 5 155
    nicotinate phosphoribosyltransferase domain containing more1, 5 82.15(n)1
    86.67(a)1
    2236461  NM_172607.31  NP_766195.21 
     AI1740345  AI8514425  (see all 11)
    chicken
    (Gallus gallus)
    NAPRT11   -- nicotinate phosphoribosyltransferase domain containing more 60.33(n)
    55.45(a)
    404301  NM_206981.1  NP_996864.1 
    About this table        Species with no ortholog for NAPRT1

    ENSEMBL Gene Tree for NAPRT1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/17 NCBI SNPs in NAPRT1 are shown (see all 17 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 60)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 8 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs8969491,2
    A,C,F144731718(-) CAAACC/GGCACG 1 -- ng312Minor allele frequency- G:0.27MN EA 1688
    --
    rs8969501,2
    A,C144731462(-) CTTGGC/TAGCCG 1 A/V mis1 ese31Minor allele frequency- T:0.00MN 184
    --
    rs100958851,2
    C144732538(+) CCACGC/TTCTGG 1 -- ng310--------
    --
    rs44755001,2
    C144731666(+) CCCCGA/GCCCCG 1 -- ng310--------
    --
    rs69961261,2
    C144731971(+) CCAGGA/CGGCGG 1 -- ng310--------
    --
    rs8969511,2
    A,C144731461(-) TTGGTA/CGCCGG 1 A/A syn1 ese30--------
    rs8969531,2
    A,C,F144731332(-) CCTTGC/TCCCCC 1 -- int11Minor allele frequency- T:0.31MN 184
    rs20155621,2
    F144731400(-) GTAGTC/ACCCCG 1 -- int11Minor allele frequency- A:0.04MN 184
    --
    rs119907681,2
    --144731414(+) CGTCCC/TGCAGG 1 R/Q mis1 ese30--------
    --
    rs615444711,2
    --144731688(+) GGCCTA/GTGGCG 1 -- ng310--------
    About this table

    HapMap Linkage Disequilibrium images for NAPRT1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 611552

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    7 PubMed articles for NAPRT1 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 93100 HGNC: 30450 AceView: PP3856 Ensembl:ENSG00000147813 euGenes: HUgn93100
    ECgene: NAPRT1 H-InvDB: NAPRT1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for NAPRT1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for NAPRT1 Antibodies for NAPRT1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for NAPRT1 Browse small molecules at Sigma-Aldrich
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     Search OriGene for NAPRT1
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     Antibodies for NAPRT1

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