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NAPG Gene

protein-coding   GIFtS: 56

GC18P010516
N-ethylmaleimide-sensitive factor attachment protein, gamma
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
GAMMASNAP 2
SNAP-gamma 3
SNAPG 3
Descriptions
N-ethylmaleimide-sensitive factor attachment protein gamma 3
N-ethylmaleimide-sensitive factor attachment protein, gamma 2
gamma SNAP 2
soluble NSF attachment protein 2
External Ids
HGNC: 76421
Entrez Gene: 87742
UniProtKB: Q997473
Ensembl: ENSG000001342657
Search outside databases for aliases for NAPG gene

Previous GC identifers: GC18P010683 GC18P010582

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for NAPG:
This gene encodes soluble NSF attachment protein gamma. The soluble NSF attachment proteins (SNAPs)
enable N-ethyl-maleimide-sensitive fusion protein (NSF) to bind to target membranes. NSF and SNAPs
appear to be general components of the intracellular membrane fusion apparatus, and their action
at specific sites of fusion must be controlled by SNAP receptors particular to the membranes being
fused. The product of this gene mediates platelet exocytosis and controls the membrane fusion
events of this process.

UniProtKB/Swiss-Prot: SNAG_HUMAN, Q99747
Function: Required for vesicular transport between the endoplasmic reticulum and the Golgi
apparatus

Gene Wiki entry for NAPG

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NAPG gene  

Entrez Gene cytogenetic band: 18p11.22   Ensembl cytogenetic band:  18p11.22   HGNC cytogenetic band: 18p11.21

NAPG Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 18         GeneLoc Exon Structure

GeneLoc location for GC18P010516:     (about GC identifiers)

Start:
10,516,031 bp from pter
End:
10,540,372 bp from pter
Size:
24,342 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000018.8  NT_010859.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: SNAG_HUMAN, Q99747 (See protein sequence)
Recommended Name: Gamma-soluble NSF attachment protein  
Size: 312 amino acids; 34746 Da
Subunit: Binds RIP11
Subcellular location: Membrane; Peripheral membrane protein (By similarity)
Secondary accessions: Q9BUV1

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_003817.1  

    ENSEMBL proteins: 
    ENSP00000324628 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (NAPG)
    Human Recombinant Proteins from Abnova (NAPG)
                  OriGene Purified Recombinant Human Protein: NAPG 

    5 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005624 membrane fraction NAS11278501
    GO:0005739 mitochondrion IDA11278501
    GO:0005783 endoplasmic reticulum IEA--
    GO:0005794 Golgi apparatus IEA--
    GO:0016020 membrane IEA--
    About this table

    Antibodies for NAPG: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for NAPG
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (NAPG), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Novus Biologicals Antibody for NAPG

    Assays for NAPG: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR000744 NSF_attach
     IPR011990 TPR-like_helical


       GeneDecks  NAPG for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q99747

    ProtoNet protein and cluster: Q99747

    UniProtKB/Swiss-Prot: SNAG_HUMAN, Q99747
    Similarity: Belongs to the SNAP family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NAPG)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NAPG)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003826

                  Applied Biosystems Silencer® siRNAs for NAPG

                  Sigma-Aldrich siRNA for NAPG  
                         Sigma-Aldrich shRNA for NAPG  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for NAPG
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003826
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003826
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_003826 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_003826

    UniProtKB/Swiss-Prot: SNAG_HUMAN, Q99747
    Function: Required for vesicular transport between the endoplasmic reticulum and the Golgi
    apparatus

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515 protein binding IPI11278501
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  NAPG  (Your Favorite Gene powered by Ingenuity) 
     Huntington's Disease Signaling

       GeneDecks  NAPG for the pathways selected above  
    About GeneDecksing

     Gene Network CentralTM Interacting Genes and Proteins Network for  NAPG 

      --

    5/6 Gene Ontology (GO) biological process terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006461 protein complex assembly NAS11278501
    GO:0006886 intracellular protein transport IEA--
    GO:0006891 intra-Golgi vesicle-mediated transport TAS8455721
    GO:0006944 membrane fusion TAS8455721
    GO:0016192 vesicle-mediated transport IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for NAPG
    2 Novoseek chemical compound relationships for NAPG gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    atp 46.53 8 8702750 (2), 12554740 (1), 17397838 (1), 9325254 (1) (see all 5)
    calcium 2.29 3 7501022 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NAPG)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NAPG)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003826

                  Sigma-Aldrich siRNA for NAPG  
                         Sigma-Aldrich shRNA for NAPG  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_003826  

    REFSEQ mRNAs for NAPG gene: 

    NM_003826.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_003826  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003826
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003826
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_003826 

    Additional cDNA sequence: 

    AF131752.1 AK023725.1 AK294038.1 AK314495.1 BC001889.1 CR536554.1 CR602036.1 U78107.1 

    9 DOTS entries:

    DT.97843470  DT.412344  DT.100775687  DT.100001089  DT.100661614  DT.100812344  DT.40213778  DT.91647160 
    DT.92422036 

    24/231 AceView cDNA sequences (see all 231 ):

    CR536554 AA767196 BC001889 AA485349 AA760853 BF060754 N90028 CA447657 
    BI760705 BQ045235 AW152374 AL559231 AK126942 R06227 AA921903 BI084064 
    AA972946 CB960558 BU173657 AW451167 BX486893 AI597642 AA903085 CA312045 

    highest scoring ESTs for NAPG:

    U78107 AA010503 AA127677 AA258463 AI159800 AI467980 AI628298 AI683876 AI942385 AL580888 

    Unigene Cluster for NAPG:

    N-ethylmaleimide-sensitive factor attachment protein, gamma
    Hs.464622  [show with all ESTs]
    Unigene Representative Sequence: NM_003826


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for NAPG

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12
    SP1:              -     -     -     -                                             
    SP2:                                                                              
    SP3:              -                                                               
    SP4:                                                                              

    About this scheme

    ECgene alternative splicing isoforms for NAPG

    1 Ensembl transcript including schematic representation:
    ENST00000322897  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    NAPG expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for NAPG

    1 / 2 / 3

    6 probe-sets matching NAPG gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    46252_at2, 3 U95-B 1 1.00 1.00 0.94 1.24 AA127677 0.80 1.00 0.91 1

    40289_at2, 3 U95-A 1 0.88 1.00 0.80 0.77 U78107 1.00 1.00 1.00 1

    225448_at2, 3 U133-B 1 1.00 1.00 -- -- AL519376 0.40 1.00 0.76 1

    210048_at2, 3 U133-A 1 1.00 1.00 -- -- BC001889 0.80 1.00 0.91 1

    225448_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    210048_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  NAPG for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AATATGCTCA

    SOURCE GeneReport for Unigene cluster: Hs.464622

    Expression variation in blood from EXPOLDB for NAPG

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for NAPG gene from 5/13 species (see all 13 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    NAPG1   -- N-ethylmaleimide-sensitive factor attachment protein, more 91.85(n)
    96.78(a)
    608647  XM_845734.1  XP_850827.1 
    chimpanzee
    (Pan troglodytes)
    NAPG1   -- N-ethylmaleimide-sensitive factor attachment protein, more 97.49(n)
    97.05(a)
    468475  XM_523864.2  XP_523864.2 
    cow
    (Bos taurus)
    NAPG1   -- N-ethylmaleimide-sensitive factor attachment protein, more 86(n)
    95.19(a)
    509041  NM_001046022.1  NP_001039487.1 
    rat
    (Rattus norvegicus)
    Napg1   -- N-ethylmaleimide-sensitive factor attachment protein, more 88.35(n)
    97.44(a)
    307382  XM_225881.4  XP_225881.4 
    mouse
    (Mus musculus)
    Napg1, 5 185
    N-ethylmaleimide sensitive fusion protein attachment more1, 5 88.25(n)1
    98.08(a)1
    1081231  NM_028017.11  NP_082293.11 
     AI4502165  AK0102755  (see all 14)
    About this table        Species with no ortholog for NAPG

    ENSEMBL Gene Tree for NAPG
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Pseudogenes
    1 related pseudogene


    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/163 NCBI SNPs in NAPG are shown (see all 163 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 140)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 18 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs5127101,2
    A,C,F,H,O10515585(-) TTAATT/CGTATG 1 -- ng517Minor allele frequency- C:0.37EU EA WA NA 554
    rs6497771,2
    A,C,F,H,O10515518(-) CCAACG/CCAAAT 1 -- ng517Minor allele frequency- C:0.33EU EA WA NA 542
    rs38653651,2
    C,F,H10515480(-) AAGTGC/TTCTAA 1 -- ng517Minor allele frequency- T:0.04EU EA WA NA 560
    rs169747651,2
    C,F,H10529774(+) AACTAC/TCAGAG 1 P/S mis1 ese315Minor allele frequency- T:0.06NA EU EA WA 1400
    rs23053701,2
    C,F,H10540121(-) TTCTTC/GTTGAT 1 K/N mis1 ese316Minor allele frequency- G:0.08NA EA EU WA 2794
    rs5445531,2
    C,F,H10514453(-) aaacaA/Tgtcat 1 -- ng518Minor allele frequency- T:0.29EU EA WA 838
    rs169747081,2
    F,H10516068(+) GTCACC/ACTCTC 1 -- ut51 ese3 trp37Minor allele frequency- A:0.02NA EU EA WA 524
    rs175471781,2
    F,H10540588(+) GAATTG/TTTTTT 1 -- ng317Minor allele frequency- T:0.01NA EU EA WA 552
    rs5728961,2
    H10514399(-) AAATAC/GTTTGG 1 -- ng514Minor allele frequency- G:0.00EU EA WA 410
    rs4962071,2
    H10520785(+) TTTTTA/TAAATG 1 L/F mis14Minor allele frequency- T:0.00EU EA WA 418
    About this table

    HapMap Linkage Disequilibrium images for NAPG (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 603216

    Human Genome Epidemiology Navigator: NAPG (3 documents)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/70 PubMed articles for NAPG gene (see all 70 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 8774 HGNC: 7642 AceView: NAPG Ensembl:ENSG00000134265 euGenes: HUgn8774
    ECgene: NAPG H-InvDB: NAPG
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for NAPG:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for NAPG-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for NAPG Antibodies for NAPG
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for NAPG Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
     Antibodies   Primer Pairs  
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     Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
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     Recombinant & Natural Proteins  

     Recombinant Proteins (NAPG)
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     Recombinant Proteins
    (NAPG)
     Antibodies (NAPG)
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for NAPG




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for NAPG

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