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NAPEPLD Gene

protein-coding   GIFtS: 44

GC07M102528
N-acyl phosphatidylethanolamine phospholipase D
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
C7orf18 1, 3
DKFZp781D1098 2
EC 3.1.4.- 3
FMP30 1, 2
NAPE-PLD 1, 2, 3
Descriptions
N-acyl phosphatidylethanolamine phospholipase D 2, 3
N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D 2
NAPE-hydrolyzing phospholipase D 3
External Ids
HGNC: 216831
Entrez Gene: 2222362
UniProtKB: Q6IQ203
Ensembl: ENSG000001610487
Search outside databases for aliases for NAPEPLD gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for NAPEPLD:
NAPEPLD is a phospholipase D type enzyme that catalyzes the release of N-acylethanolamine (NAE)
from N-acyl-phosphatidylethanolamine (NAPE) in the second step of the biosynthesis of
N-acylethanolamine (Okamoto et al., 2004 [PubMed 14634025]).[supplied by OMIM]

UniProtKB/Swiss-Prot: NAPEP_HUMAN, Q6IQ20
Function: Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs)
and phosphatidic acid. Responsible for the generation of anandamide (N-arachidonoylethanolamine),
the ligand of cannabinoid and vanilloid receptors (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 7q22.1   Ensembl cytogenetic band:  7q22.1   HGNC cytogenetic band: 7q22.1

NAPEPLD Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07M102528:     (about GC identifiers)

Start:
102,527,459 bp from pter
End:
102,576,749 bp from pter
Size:
49,291 bases
Orientation:
minus strand

1 alternative location:
Chr7-,CRA_TCAG 102,100,589-102,149,871     
RefSeq DNA sequence:
NC_000007.12  NT_007933.14  NT_079596.2  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: NAPEP_HUMAN, Q6IQ20 (See protein sequence)
Recommended Name: N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D  
Size: 393 amino acids; 45596 Da
Cofactor: Binds 1 or 2 zinc ions per subunit (By similarity)
Subunit: Monomer (By similarity)
Subcellular location: Membrane (By similarity)
Secondary accessions: Q5CZ87 Q769K1

REFSEQ proteins (2 alternative transcripts): 
NP_001116310.1  NP_945341.3  


ENSEMBL proteins: 
ENSP00000340093 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (NAPE-PLD)
Browse Abnova for Human Recombinant Proteins
              OriGene Purified Recombinant Human Protein: NAPEPLD 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016020 membrane IEA--
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Antibodies for NAPEPLD: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for NAPEPLD
Browse R&D Systems for Antibodies
Antibodies from Abcam (NAPE-PLD), each with their AbpromiseSM
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for NAPEPLD

Assays for NAPEPLD: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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ProtoNet protein and cluster: Q6IQ20

UniProtKB/Swiss-Prot: NAPEP_HUMAN, Q6IQ20
Similarity: Belongs to the NAPE-PLD family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NAPEPLD)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): XM_168636

              Applied Biosystems Silencer® siRNAs for NAPEPLD

              Sigma-Aldrich siRNA for NAPEPLD  
                     Sigma-Aldrich shRNA for NAPEPLD  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001122838
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001122838
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001122838 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_198990

UniProtKB/Swiss-Prot: NAPEP_HUMAN, Q6IQ20
Function: Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs)
and phosphatidic acid. Responsible for the generation of anandamide (N-arachidonoylethanolamine),
the ligand of cannabinoid and vanilloid receptors (By similarity)
Enzyme regulation: Activity is stimulated by divalent cations (By similarity)
Enzyme Number (IUBMB): EC 3.1.4.- 

1 MGI mutant phenotype (inferred from 2 alleles(MGI details for Napepld):

nervous system

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0008270 zinc ion binding IEA--
GO:0016787 hydrolase activity IEA--
GO:0046872 metal ion binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


1 Millipore Pathway for NAPEPLD
 Anandamide biosynthesis and metabolism

   GeneDecks  NAPEPLD for the pathways selected above  
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5 Sigma-Aldrich "Your Favorite Gene" Pathways for  NAPEPLD  (Your Favorite Gene powered by Ingenuity) 
 Phospholipid Degradation
 Glycerophospholipid Metabolism
 IL-8 Signaling
 Endothelin-1 Signaling
 Fc&gamma

   GeneDecks  NAPEPLD for the pathways selected above  
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5/6 Interacting proteins for NAPEPLD (ENSP000003400933) via UniProtKB, MINT, and/or STRING (see all 6 )
InteractantInteraction Details
GeneCardExternal ID(s)
FAAHENSP000002431673STRING (score=.748)
CNR2ENSP000003635963STRING (score=.746)
DAGLAENSP000002572153STRING (score=.738)
TRPV1ENSP000001746213STRING (score=.736)
DAGLBENSP000002970563STRING (score=.704)
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2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006629 lipid metabolic process IEA--
GO:0009395 phospholipid catabolic process IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for NAPEPLD

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NAPEPLD)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): XM_168636

              Sigma-Aldrich siRNA for NAPEPLD  
                     Sigma-Aldrich shRNA for NAPEPLD  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001122838  NM_198990  

REFSEQ mRNAs for NAPEPLD gene (2 alternative transcripts): 

NM_001122838.1   NM_198990.4   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001122838  NM_198990  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001122838
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001122838
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001122838 

Additional cDNA sequence: 

AB112352.1 AK000801.1 AK023244.1 AK304644.1 AY357337.1 BC037350.2 BC057294.1 BC071604.1 
CR615836.1 CR936639.1 

12 DOTS entries:

DT.100686975  DT.100670302  DT.99983730  DT.100710941  DT.91798318  DT.101969415  DT.102842703  DT.422759 
DT.121073908  DT.121073766  DT.40131984  DT.86841791 

24/108 AceView cDNA sequences (see all 108 ):

AA322501 BU169519 Z40218 BX091822 AI460037 AB112352 CR615836 AI209134 
BM148178 AW072626 AY357337 AA864799 AW445108 AA360843 AI399802 AI369945 
BU507368 N78417 BG742468 AI435063 H29395 BG426843 BQ025325 BQ214521 

Unigene Cluster for NAPEPLD:

N-acyl phosphatidylethanolamine phospholipase D
Hs.324271  [show with all ESTs]
Unigene Representative Sequence: NM_001122838


GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for NAPEPLD

ExUns: 1 ^ 2a · 2b · 2c ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6
SP1:                                                            
SP2:        -     -     -                                       
SP3:                    -                                       

About this scheme

ECgene alternative splicing isoforms for NAPEPLD

1 Ensembl transcript including schematic representation:
ENST00000341533  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

NAPEPLD expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for NAPEPLD

1 / 2

13 probe-sets matching NAPEPLD gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

64613_at2 U95-C 1 1.00 1.00 0.78 0.76 -- -- -- -- --

58038_at2 U95-B 1 1.00 1.00 0.79 0.88 -- -- -- -- --
43067_at2 U95-B 1 1.00 1.00 0.66 1.38 -- -- -- -- --

242635_s_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

242229_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

226041_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

238722_x_at2 U133-B 3 1.00 0.94 -- -- -- -- -- -- --

233539_at*2 U133-B 2 1.00 0.50 -- -- -- -- -- -- --

242635_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

242229_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

226041_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

238722_x_at2 U133Plus2 3 1.00 0.94 -- -- -- -- -- -- --

233539_at*2 U133Plus2 2 1.00 0.50 -- -- -- -- -- -- --
GeneDecks  NAPEPLD for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: GCAGGGGTTA

SOURCE GeneReport for Unigene cluster: Hs.324271
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for NAPEPLD gene from 5/14 species (see all 14 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
NAPEPLD1   -- N-acyl phosphatidylethanolamine phospholipase D 89.32(n)
89.74(a)
475898  XM_533105.2  XP_533105.2 
chimpanzee
(Pan troglodytes)
NAPEPLD1   -- N-acyl phosphatidylethanolamine phospholipase D 99.41(n)
98.73(a)
472476  XM_001158294.1  XP_001158294.1 
cow
(Bos taurus)
NAPEPLD1   -- N-acyl phosphatidylethanolamine phospholipase D 88.21(n)
90.51(a)
541291  NM_001015680.1  NP_001015680.1 
rat
(Rattus norvegicus)
Napepld1   -- N-acyl phosphatidylethanolamine phospholipase D 83.88(n)
90.33(a)
296757  NM_199381.1  NP_955413.1 
mouse
(Mus musculus)
Napepld1, 5 55
N-acyl phosphatidylethanolamine phospholipase D1, 5 83.88(n)1
89.06(a)1
2428641  NM_178728.41  NP_848843.11 
 AB1123505  AI4484725  (see all 12)
About this table        Species with no ortholog for NAPEPLD

ENSEMBL Gene Tree for NAPEPLD
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/292 NCBI SNPs in NAPEPLD are shown (see all 292 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 74)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 7 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs115603671,2
A,C,F,H102529380(+) GAGCAT/CTGGTG 2 -- ut31 ese34Minor allele frequency- C:0.12EU EA WA 420
rs77843371,2
A,C,F,H102528329(+) TTCACA/GAAGAG 2 -- ut31 trp312Minor allele frequency- G:0.11EU EA WA NS NA 668
--
rs31810091,2
A,C,F,H102531129(-) ATAATA/GATGAT 2 D/N mis18Minor allele frequency- G:0.14NA EU EA WA 636
rs77838561,2
A,C,F,H102528270(+) AAAGTG/ATTCCT 2 -- ut314Minor allele frequency- A:0.13EU EA WA 410
--
rs77897271,2
A,C,F,H102576731(+) CTCTCG/AGCCCG 2 -- ut514Minor allele frequency- A:0.18EU EA WA 412
rs125405831,2
C,F,H102547747(+) ACGAGA/CGCTAA 2 S/A mis1 ese311Minor allele frequency- C:0.16EU EA WA NA 982
rs132420791,2
C,F,H102529454(+) CTAACA/GTGGCA 2 -- ut31 ese36Minor allele frequency- G:0.15EU EA WA NA 602
rs132321941,2
F,H102529790(+) AAAAAT/ATATAA 2 -- ut314Minor allele frequency- A:0.43EU EA WA 418
--
rs69668761,2
A,C102578578(+) tccccA/Caaaaa 1 -- ng31 trp30--------
--
rs69629161,2
A,C102578413(+) taattA/Taaaaa 1 -- ng31 tfbs3 trp30--------
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HapMap Linkage Disequilibrium images for NAPEPLD (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 612334

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

8 PubMed articles for NAPEPLD gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 222236 HGNC: 21683 AceView: NAPE-PLD Ensembl:ENSG00000161048 euGenes: HUgn222236
ECgene: NAPEPLD H-InvDB: NAPEPLD
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
Wikipedia http://en.wikipedia.org/wiki/N-acyl_phosphatidylethanolamine-specific_phospholipase_D
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for NAPEPLD:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
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  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for NAPEPLD-related products

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 Predesigned and custom siRNAs for NAPEPLD Antibodies for NAPEPLD
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for NAPEPLD Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Tagged/untagged cDNA clones
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 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for NAPEPLD




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Search Novus for antibodies for NAPEPLD

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