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NAPB Gene

protein-coding   GIFtS: 51

GC20M023303
N-ethylmaleimide-sensitive factor attachment protein, beta
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
MGC26066 2
MGC48335 2
MSF 5
MSF1 5
OTTHUMP00000030424 2
SEPT9 5
SNAP-BETA 1, 2
SNAP-beta 3
SNAPB 1, 2, 3
Descriptions
N-ethylmaleimide-sensitive factor attachment protein beta 3
N-ethylmaleimide-sensitive factor attachment protein, beta 2
beta-soluble NSF attachment protein 2
External Ids
HGNC: 157511
Entrez Gene: 639082
UniProtKB: Q9H1153
Ensembl: ENSG000001258147
Search outside databases for aliases for NAPB gene

Previous GC identifers: GC17U990129 GC20M023350

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: SNAB_HUMAN, Q9H115
Function: Required for vesicular transport between the endoplasmic reticulum and the Golgi
apparatus (By similarity)

Gene Wiki entry for NAPB

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NAPB gene  

Entrez Gene cytogenetic band: 20p12.3-p11.21   Ensembl cytogenetic band:  20p11.21   HGNC cytogenetic band: 20p12.3-p11.21

NAPB Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20M023303:     (about GC identifiers)

Start:
23,303,165 bp from pter
End:
23,350,081 bp from pter
Size:
46,917 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000020.9  NT_011387.8  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: SNAB_HUMAN, Q9H115 (See protein sequence)
Recommended Name: Beta-soluble NSF attachment protein  
Size: 298 amino acids; 33557 Da
Subunit: Interacts with PRKCABP, and disrupts the interaction between GRIA2 and PRKCABP, leading to
the internalization of GRIA2 (By similarity)
Subcellular location: Membrane; Peripheral membrane protein (By similarity)
Secondary accessions: Q4G187 Q5JXF9 Q8N3C4

REFSEQ proteins: NP_071363.1  

ENSEMBL proteins: 
ENSP00000366225 ENSP00000381459 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (alpha+beta SNAP)
Browse Abnova for Human Recombinant Proteins
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005783 endoplasmic reticulum IEA--
GO:0005794 Golgi apparatus IEA--
GO:0016020 membrane IEA--
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Antibodies for NAPB: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibody Arrays and Antibodies for NAPB
Browse R&D Systems for Antibodies
Antibodies from Abcam (alpha+beta SNAP), each with their AbpromiseSM
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for NAPB

Assays for NAPB: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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2 InterPro domains/families:
 IPR000744 NSF_attach
 IPR011990 TPR-like_helical


   GeneDecks  NAPB for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q9H115

ProtoNet protein and cluster: Q9H115

1 Blocks protein family: IPB000744 NSF attachment protein signature

UniProtKB/Swiss-Prot: SNAB_HUMAN, Q9H115
Similarity: Belongs to the SNAP family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NAPB)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_022080

              Applied Biosystems Silencer® siRNAs for NAPB

              Sigma-Aldrich siRNA for NAPB  
                     Sigma-Aldrich shRNA for NAPB  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for NAPB
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_022080
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_022080
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_022080 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_022080

UniProtKB/Swiss-Prot: SNAB_HUMAN, Q9H115
Function: Required for vesicular transport between the endoplasmic reticulum and the Golgi
apparatus (By similarity)

1 Gene Ontology (GO) molecular function term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005488 binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Sigma-Aldrich "Your Favorite Gene" Pathway for  NAPB  (Your Favorite Gene powered by Ingenuity) 
 Huntington's Disease Signaling

   GeneDecks  NAPB for the pathways selected above  
About GeneDecksing

  --

2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006886 intracellular protein transport IEA--
GO:0016192 vesicle-mediated transport IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for NAPB

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NAPB)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_022080

              Sigma-Aldrich siRNA for NAPB  
                     Sigma-Aldrich shRNA for NAPB  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_022080  

REFSEQ mRNAs for NAPB gene: 

NM_022080.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_022080  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_022080
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_022080
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_022080 

Additional cDNA sequence: 

AK022817.1 AK124876.1 AK294703.1 AK295791.1 AK296385.1 AK307709.1 AL834445.1 BC008463.1 
BC026310.1 BC047426.1 BC060840.1 BX648330.1 

15 DOTS entries:

DT.449099  DT.101983121  DT.101983122  DT.99946862  DT.101983124  DT.100031457  DT.100781796  DT.97786086 
DT.120839147  DT.120839159  DT.97842873  DT.120839158  DT.120839173  DT.120839174  DT.120839182 

24/203 AceView cDNA sequences (see all 203 ):

BE327058 AI278427 AK124876 BM717893 BM128314 AK125811 CB069717 BU184334 
BM699534 BM704222 AA351671 AA953489 AL834445 H91664 CK819291 CB127322 
AW207670 BM930893 AI692909 BQ694031 F13158 F09971 T33462 AI305251 

highest scoring ESTs for NAPB:

AA083398 AI915331 AL713203 AW207670 AW207721 BG704245 BG704312 BI458311 BI544957 BI545009 

Unigene Cluster for NAPB:

N-ethylmaleimide-sensitive factor attachment protein, beta
Hs.269471  [show with all ESTs]
Unigene Representative Sequence: NM_022080


GeneLoc Exon Structure

5/16 Alternative Splicing Database (ASD) splice patterns (SP) for NAPB (see all 16 )

ExUns: 1a · 1b · 1c · 1d · 1e ^ 2a · 2b ^ 3a · 3b · 3c ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b ^ 9a · 9b · 9c ^ 10 ^ 11 ^ 12a · 12b ^ 13a ·
SP1:                                      -                 -     -     -                                         -                 -                 -         
SP2:                                      -     -           -     -     -                                         -                 -                 -         
SP3:                                      -     -           -     -     -     -                                   -                 -                 -         
SP4:                                      -     -           -     -     -     -                                   -                 -                 -         
SP5:                                                                                                              -                 -                 -         

ExUns: 13b · 13c · 13d
SP1:                  
SP2:                  
SP3:                  
SP4:                  
SP5:                  

About this scheme

ECgene alternative splicing isoforms for NAPB

2 Ensembl transcripts including schematic representations:
ENST00000377026  ENST00000398425  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

NAPB expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for NAPB

1 / 2 / 3

4 probe-sets matching NAPB gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

53776_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AA044775 0.60 1.00 0.82 1

225111_s_at2, 3 U133-B 1 1.00 1.00 -- -- AK022817 0.60 0.75 0.68 1

1570441_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

225111_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TGTAACAATA

SOURCE GeneReport for Unigene cluster: Hs.269471
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for NAPB gene from 5/12 species (see all 12 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
NAPB1   -- N-ethylmaleimide-sensitive factor attachment protein, more 93.85(n)
99.66(a)
477131  XM_534322.2  XP_534322.2 
chimpanzee
(Pan troglodytes)
NAPB1   -- N-ethylmaleimide-sensitive factor attachment protein, more 99.89(n)
100(a)
458139  XM_514551.2  XP_514551.2 
cow
(Bos taurus)
NAPB1   -- N-ethylmaleimide-sensitive factor attachment protein, more 89.15(n)
99.33(a)
517728  NM_001046233.1  NP_001039698.1 
rat
(Rattus norvegicus)
Napb1   -- N-ethylmaleimide-sensitive factor attachment protein, more 92.06(n)
100(a)
499903  XM_575249.2  XP_575249.1 
mouse
(Mus musculus)
Napb1, 5 25
N-ethylmaleimide sensitive fusion protein attachment more1, 5 91.16(n)1
100(a)1
179571  NM_019632.21  NP_062606.11 
 AK0392045  AK0439175  (see all 14)
About this table        Species with no ortholog for NAPB

ENSEMBL Gene Tree for NAPB
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for NAPB gene
NAPA2  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/390 NCBI SNPs in NAPB are shown (see all 390 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 131)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 20 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs22528241,2
A,C,F,H,O23302748(+) GTTGAG/ATGGTA 1 -- ng5116Minor allele frequency- A:0.33EA EU WA NA 2888
rs24245521,2
A,C,F,H,O23350833(+) TAGGAG/AAAGTA 1 -- ng314Minor allele frequency- A:0.24EU EA WA 412
rs24245531,2
A,C,F,H23351939(+) GAAGAA/GTAGAA 1 -- ng31 trp35Minor allele frequency- G:0.48EU EA NA 482
rs61326051,2
A,C,F,H23302839(+) TTTGCA/CAGTAA 1 -- ng517Minor allele frequency- C:0.15EU EA WA NA 548
--
rs20247461,2
A,C,F,O23304862(+) AATAGA/GATCAA 1 -- ut31 ese33Minor allele frequency- G:0.26NA 142
--
rs86151,2
A,C,F,H23303423(-) GTGGCG/ATCACT 1 -- ut31 ese310Minor allele frequency- A:0.17MN EU EA WA 1204
rs61379131,2
A,C,F,H23305725(+) CATAGG/ACTCCA 1 -- ut31 ese311Minor allele frequency- A:0.15EU EA WA NA 976
rs38270851,2
C,F,H23304985(+) GAAACA/TAGTTG 1 -- ut314Minor allele frequency- T:0.15EU EA WA 412
--
rs24245271,2
A,C23305329(+) GTGTAA/CACGCG 1 -- ut31 ese30--------
--
rs7617251,2
C,F23350735(-) AATGGC/TTCACA 1 -- ng31 tfbs31Minor allele frequency- T:0.09MN 184
About this table

HapMap Linkage Disequilibrium images for NAPB (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 604061

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

9 PubMed articles for NAPB gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 63908 HGNC: 15751 AceView: NAPB Ensembl:ENSG00000125814 euGenes: HUgn63908
ECgene: NAPB H-InvDB: NAPB
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for NAPB:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
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  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for NAPB-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for NAPB Antibodies for NAPB
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for NAPB Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant Proteins
(alpha+beta SNAP)
 Antibodies (alpha+beta SNAP)
 Search OriGene for NAPB
 Search Tocris compounds for NAPB




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Search Novus for antibodies for NAPB

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