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NAP5 Gene

protein-coding GIFtS: 34

GC02M133145

Nck-associated protein 5
symbol from the EntrezGene Database
NOT approved by the HUGO Gene Nomenclature Committee (HGNC).

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
ERIH ^³
ERIH2 ^²
FLJ34870 ^²
NAP-5 ^³

Descriptions
Nck-associated protein 5 ^²
Peripheral clock protein ^³
peripheral clock protein 2 ^²

External Ids
Entrez Gene: 344148²
UniProtKB: O14513³
Ensembl: ENSG00000176771⁷

Search outside databases for aliases for NAP5 gene

Previous GC identifers: GC02M133538 GC02M133741 GC02M133640 GC02M133263

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2q21.2 Ensembl cytogenetic band: 2q21.2

NAP5 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2 GeneLoc Exon Structure

GeneLoc location for GC02M133145: (about GC identifiers)

Start:	133,145,842 bp from pter
End:	134,042,504 bp from pter
Size:	896,663 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000002.10 NT_022135.15

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: NAP5_HUMAN, O14513 (See protein sequence)

Recommended Name: Nck-associated protein 5

Size: 1909 amino acids; 208537 Da

Subunit: Interacts with the SH3-containing region of the adapter protein Nck

Caution: It is uncertain whether Met-1 or Met-26 is the initiator

Sequence caution: Sequence=BAA22433.1; Type=Frameshift; Positions=856, 877, 920, 926, 957, 967;

Secondary accessions: Q29SS9 Q29ST0 Q2NL90 Q6ZVE2 Q8NAS3

Alternative splicing: 4 isoforms: O14513-1 O14513-2 O14513-3 O14513-4

Post-translational modifications:

View phosphorylation sites using PhosphoSite²

REFSEQ proteins (2 alternative transcripts):

NP_997246.2 NP_997364.3

ENSEMBL proteins:

ENSP00000387128 ENSP00000386952 ENSP00000385692 ENSP00000380603 ENSP00000384489 ENSP00000351882

Human Recombinant Proteins

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	Browse Abcam for Recombinant Proteins
	Browse Abnova for Human Recombinant Proteins

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

1 Gene Ontology (GO) cellular component term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005575	cellular_component	ND	--

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Antibodies for NAP5:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for NAP5

Assays for NAP5:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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ProtoNet protein and cluster: O14513

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Browse Invitrogen for RNAi
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 5): NM_207481

Applied Biosystems Silencer® siRNAs for NAP5

Sigma-Aldrich siRNA for NAP5

Sigma-Aldrich shRNA for NAP5

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_207363
Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_207363
untagged cDNA clones in CMV expression vector (see all 3): NM_207363

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_207363

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003674	molecular_function	ND	--
GO:0005515	protein binding	IPI	17474147

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Gene Network Central^TM Interacting Genes and Proteins Network for NAP5

4 Interacting proteins for NAP5 (O14513¹) via UniProtKB, MINT, and/or STRING
Interactant Interaction Details
GeneCard External ID(s)
ABL1 P00519¹ EBI-1752508, EBI-375543
FYN P06241¹ EBI-1752508, EBI-515315
GRB2 P62993¹ EBI-1752508, EBI-401755
NCK1 P16333¹ EBI-1752508, EBI-389883
About this table
1 Gene Ontology (GO) biological process term (links to tree view):
GO ID Qualified GO term Evidence PubMed IDs
GO:0008150 biological_process ND --
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for NAP5

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Browse Invitrogen for RNAi
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 5): NM_207481

Sigma-Aldrich siRNA for NAP5

Sigma-Aldrich shRNA for NAP5

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_207363 NM_207481

REFSEQ mRNAs for NAP5 gene (2 alternative transcripts):

NM_207363.2 NM_207481.3

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_207363 NM_207481

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_207363
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_207363
                                 untagged cDNA clones in CMV expression vector (see all 3): NM_207363

Additional cDNA sequence:

AB005217.1 AK057980.1 AK092189.1 AK124659.1 AY946007.1 AY946008.1 BC110831.1

9 DOTS entries:

DT.92066581 DT.40116151 DT.75157428 DT.91716271 DT.40115256 DT.95233567 DT.40312617 DT.40115255

DT.40199397

22 AceView cDNA sequences:

CA438539 BF940247 AI590295 BI496664 AI367833 AI347863 BI494911 BM972282

BU737153 BX112078 BE858593 AK124659 NM_207363 BU186341 R54936 AA053381

AI830450 BI494910 BI496665 AA779875 R54748 BG200485

highest scoring ESTs for NAP5:

BE619570 BE963800 NM_207363 XM_292943 AI367833 BF358526 BF869490 BG200485 BU737153 AA121579

Unigene Cluster for NAP5:

Nck-associated protein 5

Hs.537329 [show with all ESTs]

Unigene Representative Sequence: NM_207363

GeneLoc Exon Structure

6 Ensembl transcripts including schematic representations:

ENST00000409261 ENST00000409213 ENST00000405974 ENST00000317721 ENST00000407235 ENST00000358991

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

NAP5 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for NAP5

¹ / ² / ³

5 probe-sets matching NAP5 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
81307_i_at²^{, 3}	U95-D	1	1.00	1.00	0.69	1.25	AI830450	0.40	0.50	0.45	1
81309_r_at²^{, 3}	U95-D	1	1.00	1.00	0.64	0.83	AI830450	0.40	0.50	0.45	1
87928_at²^{, 3}	U95-D	1	1.00	1.00	0.33	0.96	AA121384	0.20	0.50	0.38	1
239650_at²^{, 3}	U133-B	1	1.00	1.00	--	--	BE858593	0.20	0.50	0.38	1
239650_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

GeneDecks NAP5 for binary patterns associated with the probe-sets selected above

About GeneDecksing

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: CTCTTGAAAT

SOURCE GeneReport for Unigene cluster: Hs.537329
UniProtKB/Swiss-Prot: NAP5_HUMAN, O14513
Tissue specificity: Expressed in fetal and adult brain, leukocytes and fetal fibroblasts

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for NAP5 gene from 5 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	LOC483893¹	--	similar to Nck-associated protein 5	81.58(n) 75.53(a)	483893 XM_541013.2 XP_541013.2
chimpanzee (Pan troglodytes)	LOC460094¹	--	Nck-associated protein 5	96.93(n) 96.85(a)	460094 XM_001151197.1 XP_001151197.1
rat (Rattus norvegicus)	RGD1564507¹	--	similar to Nck-associated protein 5	70.25(n) 60.11(a)	363974 XM_344134.3 XP_344135.3
mouse (Mus musculus)	E030049G20Rik¹	--	RIKEN cDNA E030049G20 gene	75.67(n) 68.01(a)	210356 NM_001081756.1 NP_001075225.1
chicken (Gallus gallus)	LOC424283¹	--	similar to peripheral clock protein 2	64.19(n) 57.06(a)	424283 XM_422129.2 XP_422129.2

About this table Species with no ortholog for NAP5

ENSEMBL Gene Tree for NAP5

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for NAP5 gene

KIAA1602²

Pseudogenes

1 related pseudogene

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/4460 NCBI SNPs in NAP5 are shown (see all 4460 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 1577)

AB	SNP ID	Valid	Chr 2 pos	Sequence	Recs	AA Chg	Type	More	Recs	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--			--
	rs978097^1,2	A,C,F,H	134044181(+)	`GCAGCG/ATAGAA`	2	--	ng3¹		4	NA EU	242
	rs11675147^1,2	A,C,F,H	134043638(+)	`ATTGAG/AGGTGG`	2	--	ng3¹		4	EU EA WA	378
	rs12691830^1,2	A,C,F,H	133257924(+)	`CAGAAA/GTTCCT`	2	I/T	mis¹ int¹ ese³		8	EA NA EU WA	628
	rs2278752^1,2	C,F,H,O	133256646(-)	`GGCTGT/CACTTG`	2	A/V	mis¹ int¹		8	EA NA EU WA	2040
	rs6430430^1,2	A,C,F,H	134042279(+)	`GTCAAC/TACATA`	2	--	ut5¹		4	EU EA WA	418
	rs12611515^1,2	A,C,F,H	133258045(+)	`GGAGAC/TGGACC`	2	V/I	mis¹ int¹ ese³		4	EU EA WA	412
	rs6737180^1,2	A,C,F,H	134044241(+)	`ATATAA/GATGAT`	2	--	ng3¹		7	EU EA WA NA	562
	rs17325719^1,2	C,F,H	133259055(+)	`AAGGAC/GTTTTC`	2	S/T	mis¹ int¹		12	NA EA EU WA	1054
	rs13421559^1,2	C,F,H	134042993(+)	`GTCTCA/GGCTTG`	2	--	ng3¹		11	EU EA WA NA	980
	rs16841277^1,2	C,F,H	133257577(+)	`ATCATT/ACAATT`	2	Y/N	mis¹ int¹		12	NA EU EA WA	1048

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HapMap Linkage Disequilibrium images for NAP5 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 608789

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

6 PubMed articles for NAP5 gene:

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{3, 4} Ota T....Sugano S. (2004)
A novel ligand for an SH3 domain of the adaptor protein Nck bears an SH2 domain and nuclear signaling motifs. (PubMed id 9344857)^{3, 4} Matuoka K.... Takenawa T. (1997)
Genome-wide association analysis of susceptibility and clinical phenotype in multiple sclerosis. (PubMed id 19010793)³ Baranzini S.E....Oksenberg J.R. (2008)
Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening. (PubMed id 17474147)³ Wu C....Li S.S. (2007)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 344148

AceView: NAP5

Ensembl:ENSG00000176771

euGenes: HUgn344148

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for NAP5
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for NAP5:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for NAP5-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for NAP5		Browse antibodies at Sigma-Aldrich
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NAP5 Gene

protein-coding GIFtS: 34

Aliases &
Descriptions
for NAP5

Summaries
for NAP5

Genomic Location
for NAP5

Proteins
for NAP5

Protein Domains/
Families
for NAP5

Gene Function
for NAP5

Pathways & Interactions
for NAP5

Drugs & Compounds
for NAP5

Transcripts
for NAP5

Expression
for NAP5

Orthologs
for NAP5

Paralogs
for NAP5

SNPs/Variants
for NAP5

Disorders & Mutations
for NAP5

Medical News
for NAP5

Publications
for NAP5

Search for NAP5

Genome Databases showing NAP5

Other Databases showing NAP5

Specialized Databases showing NAP5

Licensable Technologies
for NAP5

Services
for NAP5

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

NAP5 Gene

protein-coding GIFtS: 34

Aliases & Descriptionsfor NAP5

Summariesfor NAP5

Genomic Locationfor NAP5

Proteinsfor NAP5

Protein Domains/Familiesfor NAP5

Gene Functionfor NAP5

Pathways & Interactionsfor NAP5

Drugs & Compoundsfor NAP5

Transcriptsfor NAP5

Expression for NAP5

Orthologsfor NAP5

Paralogsfor NAP5

SNPs/Variantsfor NAP5

Disorders & Mutationsfor NAP5

Medical Newsfor NAP5

Publicationsfor NAP5

Search for NAP5

Genome Databases showing NAP5

Other Databases showing NAP5

Specialized Databases showing NAP5

Licensable Technologiesfor NAP5

Servicesfor NAP5

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for NAP5

Summaries
for NAP5

Genomic Location
for NAP5

Proteins
for NAP5

Protein Domains/
Families
for NAP5

Gene Function
for NAP5

Pathways & Interactions
for NAP5

Drugs & Compounds
for NAP5

Transcripts
for NAP5

Expression
for NAP5

Orthologs
for NAP5

Paralogs
for NAP5

SNPs/Variants
for NAP5

Disorders & Mutations
for NAP5

Medical News
for NAP5

Publications
for NAP5

Licensable Technologies
for NAP5

Services
for NAP5