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NAP1L4 Gene

protein-coding   GIFtS: 62

GC11M002922
nucleosome assembly protein 1-like 4
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
MGC4565 2
NAP2 1, 2, 3, 5
NAP2L 2, 5
OTTHUMP00000012671 2
hNAP2 2
Descriptions
Nucleosome assembly protein 2 2, 3
nucleosome assembly protein 1-like 4 2
External Ids
HGNC: 76401
Entrez Gene: 46762
UniProtKB: Q997333
Ensembl: ENSG000002055317
Search outside databases for aliases for NAP1L4 gene

Previous GC identifers: GC11M003025 GC11M003216 GC11M002924 GC11M002929 GC11M002919

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for NAP1L4:
This gene encodes a member of the nucleosome assembly protein (NAP) family which can interact with
both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role
as a histone chaperone. This gene is one of several located near the imprinted gene domain of
11p15.5, an important tumor-suppressor gene region. Alterations in this region have been
associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical
carcinoma, and lung, ovarian, and breast cancer. [provided by RefSeq]

Gene Wiki entry for NAP1L4
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NAP1L4 gene  

Entrez Gene cytogenetic band: 11p15.5   Ensembl cytogenetic band:  11p15.4   HGNC cytogenetic band: 11p15.5

NAP1L4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11M002922:     (about GC identifiers)

Start:
2,922,236 bp from pter
End:
2,970,183 bp from pter
Size:
47,948 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000011.8  NT_009237.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: NP1L4_HUMAN, Q99733 (See protein sequence)
Recommended Name: Nucleosome assembly protein 1-like 4  
Size: 375 amino acids; 42823 Da
Subcellular location: Nucleus

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_005960.1  

    ENSEMBL proteins: 
    ENSP00000382533 ENSP00000382523 ENSP00000369915 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Sigma-Aldrich AQUA Peptides for NAP1L4  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (NAP1L4)
    Human Recombinant Proteins from Abnova (NAP1L4)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    GO:0005678 chromatin assembly complex TAS9325046
    GO:0005737 cytoplasm TAS9325046
    About this table

    Antibodies for NAP1L4: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (NAP1L4), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (NAP1L4)
    Novus Biologicals Antibody for NAP1L4

    Assays for NAP1L4: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR002164 NAP_family


       GeneDecks  NAP1L4 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q99733

    ProtoNet protein and cluster: Q99733

    1 Blocks protein family: IPB002164 Nucleosome assembly protein (NAP)

    UniProtKB/Swiss-Prot: NP1L4_HUMAN, Q99733
    Similarity: Belongs to the nucleosome assembly protein (NAP) family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NAP1L4)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NAP1L4)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005969

                  Applied Biosystems Silencer® siRNAs for NAP1L4

                  Sigma-Aldrich siRNA for NAP1L4  
                         Sigma-Aldrich shRNA for NAP1L4  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005969
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005969
                                     untagged cDNA clones in CMV expression vector (see all 2): AK095320 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_005969

    Genatlas biochemistry entry for NAP1L4:
    nucleosome assembly protein 1-like 4, excluded of the imprinting domain,interacting with core and
    linker histones,putative chaperone

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0051082 unfolded protein binding TAS9325046
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    5/15 Interacting proteins for NAP1L4 (ENSP000003699153) via UniProtKB, MINT, and/or STRING (see all 15 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CHAF1AENSP000003012803STRING (score=.899)
    CHAF1BENSP000003157003STRING (score=.899)
    NAP1L1ENSP000002611823STRING (score=.899)
    PHLDA2ENSP000003192313STRING (score=.863)
    PPBPENSP000002960283STRING (score=.829)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006334 nucleosome assembly TAS9325046
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for NAP1L4

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NAP1L4)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NAP1L4)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005969

                  Sigma-Aldrich siRNA for NAP1L4  
                         Sigma-Aldrich shRNA for NAP1L4  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_005969  

    REFSEQ mRNAs for NAP1L4 gene: 

    NM_005969.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_005969  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005969
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005969
                                     untagged cDNA clones in CMV expression vector (see all 2): AK095320 

    Additional cDNA sequence: 

    AK299515.1 AK311098.1 AK312599.1 AK316242.1 AK316324.1 AK316548.1 BC022090.2 CR624939.1 
    U77456.1 

    24/29 DOTS entries (see all 29 ):

    DT.448916  DT.95197346  DT.100798067  DT.92462968  DT.120697094  DT.97769343  DT.100811147  DT.92462973 
    DT.86854900  DT.439912  DT.120697081  DT.120697111  DT.101968316  DT.91745205  DT.92063326  DT.95192027 
    DT.95197340  DT.120697077  DT.40298417  DT.95360129  DT.100798070  DT.102842833  DT.120697088  DT.120697146 

    24/569 AceView cDNA sequences (see all 569 ):

    N71060 BQ653221 AI350273 BM765931 BE253491 CB108970 BI087561 BM710883 
    BG820129 BQ219178 BX394357 BM982895 AI635549 BX479631 AI373211 AA448113 
    BM799809 AW246975 BQ027034 BI861430 BM724553 NM_005969 BM695399 AU138864 

    highest scoring ESTs for NAP1L4:

    U77456 AU099131 BI601589 BM834137 BU598621 CA338105 AL135252 AU130576 AU131441 AU139058 

    Unigene Cluster for NAP1L4:

    Nucleosome assembly protein 1-like 4
    Hs.501684  [show with all ESTs]
    Unigene Representative Sequence: NM_005969


    GeneLoc Exon Structure

    3 Ensembl transcripts including schematic representations:
    ENST00000399624  ENST00000399614  ENST00000380542  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    NAP1L4 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for NAP1L4

    1 / 2 / 3

    8 probe-sets matching NAP1L4 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    32575_at2, 3 U95-A 1 1.00 1.00 0.41 1.53 U77456 1.00 0.90 0.95 1

    50654_at2, 3 U95-C 1 1.00 1.00 0.90 0.50 AA775427 0.60 1.00 0.82 1

    244001_at2, 3 U133-B 1 1.00 1.00 -- -- BF960295 0.40 1.00 0.76 1

    201414_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_005969 0.60 1.00 0.82 1

    244001_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    201414_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1556567_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1560339_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GCATAGCTGG

    SOURCE GeneReport for Unigene cluster: Hs.501684

    Expression variation in blood from EXPOLDB for NAP1L4

    UniProtKB/Swiss-Prot: NP1L4_HUMAN, Q99733
    Tissue specificity: Ubiquitous. Biallelically expressed in fetal and adult tissues. Highest levels
    in testis

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for NAP1L4 gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    NAP1L41   -- nucleosome assembly protein 1-like 4 88.95(n)
    95.72(a)
    483670  XM_540791.2  XP_540791.2 
    chimpanzee
    (Pan troglodytes)
    NAP1L41   -- nucleosome assembly protein 1-like 4 99.02(n)
    99.2(a)
    450961  XM_508227.2  XP_508227.2 
    cow
    (Bos taurus)
    NAP1L41   -- nucleosome assembly protein 1-like 4 87.34(n)
    94.12(a)
    513028  NM_001038094.1  NP_001033183.1 
    rat
    (Rattus norvegicus)
    Nap1l41   -- nucleosome assembly protein 1-like 4 85.92(n)
    94.12(a)
    361684  NM_001012170.1  NP_001012170.1 
    mouse
    (Mus musculus)
    Nap1l41, 5 7 (69.55 cM)5
    nucleosome assembly protein 1-like 41, 5 85.87(n)1
    94.67(a)1
    179551  NM_008672.21  NP_032698.11 
     AA4075845  AI3167765  (see all 19)
    About this table        Species with no ortholog for NAP1L4

    ENSEMBL Gene Tree for NAP1L4
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for NAP1L4 gene
    NAP1L62  NAP1L32  NAP1L22  NAP1L52  NAP1L12  

    Pseudogenes
    2 related pseudogenes


    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/371 NCBI SNPs in NAP1L4 are shown (see all 371 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 145)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 11 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs85051,2
    A,C,F2922869(-) TTCTTG/CATTTT 1 -- ut312Minor allele frequency- C:0.36MN 372
    --
    rs74840071,2
    A,C2971959(+) aaaaaA/Taaaaa 1 -- ng31 tfbs30--------
    --
    rs106701,2
    C,F2922304(+) CTGTAT/ATCAGT 1 -- ut31 ese31Minor allele frequency- A:0.04MN 184
    --
    rs10505511,2
    F2922368(-) TGGCCG/TTGTCC 1 -- ut31 ese31Minor allele frequency- T:0.06MN 184
    --
    rs10505521,2
    F2922345(-) CCTGTG/AGAGAG 1 -- ut31 ese31Minor allele frequency- A:0.08MN 184
    rs25191701,2
    H2932385(+) GCGGGA/GCTATC 1 V/A mis1 ese34Minor allele frequency- G:0.00EU EA WA 420
    rs122861201,2
    A2921828(+) GTGCCG/TGTGCA 1 -- ng510--------
    --
    rs11310861,2
    F2932454(-) ATGGAA/GAATCA 3 DEDSEFT QDEDSEFT RDEDSEFT fra1 mis10--------
    rs128002381,2
    H2932347(+) ATCTTC/TTATGG 1 E/K mis1 ese34Minor allele frequency- T:0.00EU EA WA 406
    --
    rs47586231,2
    C2971529(+) aggccC/Tgtaat 1 -- ng310--------
    About this table

    HapMap Linkage Disequilibrium images for NAP1L4 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 601651

    3 Novoseek disease relationships for NAP1L4 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    beckwith-wiedemann syndrome 88.46 1 10777363 (1)
    wilms tumor 68.25 1 9325046 (1)
    tumors 8.64 1 9325046 (1)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/25 PubMed articles for NAP1L4 gene (see all 25 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 4676 HGNC: 7640 AceView: NAP1L4 Ensembl:ENSG00000205531 euGenes: HUgn4676
    ECgene: NAP1L4 H-InvDB: NAP1L4
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for NAP1L4 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for NAP1L4:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for NAP1L4-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for NAP1L4 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Proteins for NAP1L4
     Lentivirus-delivered shRNAs for NAP1L4 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Search OriGene for NAP1L4
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     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for NAP1L4

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