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NAP1L3 Gene

protein-coding   GIFtS: 50

GC0XM092732
nucleosome assembly protein 1-like 3
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
BNAP 3
MB20 1, 2
MGC26312 1, 2
NPL3 1, 2
OTTHUMP00000023645 2
Descriptions
nucleosome assembly protein 1-like 3 2
External Ids
HGNC: 76391
Entrez Gene: 46752
UniProtKB: Q994573
Ensembl: ENSG000001863107
Search outside databases for aliases for NAP1L3 gene

Previous GC identifers: GC0XM088209 GC0XM089906 GC0XM090961 GC0XM091697

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for NAP1L3:
This gene encodes a member of the nucleosome assembly protein (NAP) family. It is linked closely to
a region of genes responsible for several X-linked mental retardation syndromes. Alternatively
spliced transcript variants have been found for this gene, but the biological validity of the
variants has not been determined. [provided by RefSeq]

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NAP1L3 gene  

Entrez Gene cytogenetic band: Xq21.3-q22   Ensembl cytogenetic band:  Xq21.32   HGNC cytogenetic band: Xq21.3-q22

NAP1L3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XM092732:     (about GC identifiers)

Start:
92,812,585 bp from pter
End:
92,815,264 bp from pter
Size:
2,680 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000023.9  NT_011651.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: NP1L3_HUMAN, Q99457 (See protein sequence)
Recommended Name: Nucleosome assembly protein 1-like 3  
Size: 506 amino acids; 57593 Da
Subcellular location: Nucleus (By similarity)
Secondary accessions: B2RCM0 O60788

REFSEQ proteins: NP_004529.2  

ENSEMBL proteins: 
ENSP00000362171 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (NAP1L3)
Human Recombinant Proteins from Abnova (NAP1L3)
              OriGene Purified Recombinant Human Protein: NAP1L3 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IEA--
GO:0005678 chromatin assembly complex TAS8976385
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Antibodies for NAP1L3: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for NAP1L3
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Antibodies from Abcam (NAP1L3), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (NAP1L3)
Novus Biologicals Antibodies for NAP1L3

Assays for NAP1L3: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR002164 NAP_family


   GeneDecks  NAP1L3 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q99457

ProtoNet protein and cluster: Q99457

1 Blocks protein family: IPB002164 Nucleosome assembly protein (NAP)

UniProtKB/Swiss-Prot: NP1L3_HUMAN, Q99457
Similarity: Belongs to the nucleosome assembly protein (NAP) family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NAP1L3)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004538

              Applied Biosystems Silencer® siRNAs for NAP1L3

              Sigma-Aldrich siRNA for NAP1L3  
                     Sigma-Aldrich shRNA for NAP1L3  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for NAP1L3
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_004538
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_004538
                                 untagged cDNA clone in CMV expression vector: NM_004538 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_004538

Genatlas biochemistry entry for NAP1L3:
nucleosome assembly protein-like 3,tightly linked to DXS990
1 MGI phenotypic allele for Nap1l3 (no phenotypes)

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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  --

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006334 nucleosome assembly TAS8976385
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for NAP1L3

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NAP1L3)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004538

              Sigma-Aldrich siRNA for NAP1L3  
                     Sigma-Aldrich shRNA for NAP1L3  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_004538  

REFSEQ mRNAs for NAP1L3 gene: 

NM_004538.4   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_004538  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_004538
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_004538
                                 untagged cDNA clone in CMV expression vector: NM_004538 

Additional cDNA sequence: 

AK090772.1 AK095682.1 AK223621.1 AK315176.1 BC034954.2 BC094729.1 CR610053.1 D50370.1 

5 DOTS entries:

DT.92423957  DT.116325  DT.121298700  DT.121298694  DT.121298688 

24/84 AceView cDNA sequences (see all 84 ):

NM_004538 BF594742 AI241936 AU127955 AI418649 AL119710 BG654940 AA393645 
AL534617 CR610053 AL120635 AI015526 BQ637558 AI370896 AA400830 D50370 
AA205224 BG699951 BX413252 AA043992 AA626771 AL536140 AV729387 AU131171 

highest scoring ESTs for NAP1L3:

D50370 AA223829 AA393645 AA463421 AI207857 AI333607 AL119710 AL120635 AU127955 AU131171 

Unigene Cluster for NAP1L3:

Nucleosome assembly protein 1-like 3
Hs.21365  [show with all ESTs]
Unigene Representative Sequence: BC094729


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000373079  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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NAP1L3 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for NAP1L3

1 / 2 / 3

3 probe-sets matching NAP1L3 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
743_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 D50370 1.00 1.00 1.00 1

204749_at2, 3 U133-A 1 1.00 1.00 -- -- NM_004538 0.60 1.00 0.82 1

204749_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  NAP1L3 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TTCTTGTATT

SOURCE GeneReport for Unigene cluster: Hs.21365

Expression variation in blood from EXPOLDB for NAP1L3

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for NAP1L3 gene from 4 species
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
NAP1L31   -- nucleosome assembly protein 1-like 3 87.71(n)
83.26(a)
492003  XM_549123.2  XP_549123.2 
chimpanzee
(Pan troglodytes)
NAP1L31   -- nucleosome assembly protein 1-like 3 99.6(n)
99.21(a)
465745  XM_001136245.1  XP_001136245.1 
rat
(Rattus norvegicus)
Nap1l31   -- nucleosome assembly protein 1-like 3 82.24(n)
76.94(a)
170914  NM_133402.2  NP_596893.1 
mouse
(Mus musculus)
Nap1l31, 5 X5
nucleosome assembly protein 1-like 31, 5 82.67(n)1
78.57(a)1
545611  NM_138742.11  NP_620081.11 
 AB0107115  AK0785215  (see all 9)
About this table        Species with no ortholog for NAP1L3

ENSEMBL Gene Tree for NAP1L3
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for NAP1L3 gene
NAP1L62  NAP1L22  NAP1L52  NAP1L42  NAP1L12  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
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  --
(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
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OMIM: 300117

(Possibly Related Articles in Doctor's Guide)
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  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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7 PubMed articles for NAP1L3 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 4675 HGNC: 7639 AceView: NAP1L3 Ensembl:ENSG00000186310 euGenes: HUgn4675
ECgene: NAP1L3 H-InvDB: NAP1L3
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for NAP1L3:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Millipore Preclinical / Clinical Development Services
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 Predesigned and custom siRNAs for NAP1L3 Antibodies for NAP1L3
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for NAP1L3 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant Proteins
(NAP1L3)
 Antibodies (NAP1L3)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for NAP1L3




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for NAP1L3

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