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NAP1L2 Gene

protein-coding GIFtS: 50

GC0XM072348

nucleosome assembly protein 1-like 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
BPX ^¹, ², ³
MGC26243 ^¹, ²
OTTHUMP00000023565 ^²

Descriptions
Brain-specific protein, X-linked ^³
brain specific gene BPX ^²
nucleosome assembly protein 1-like 2 ^²

External Ids
HGNC: 7638¹
Entrez Gene: 4674²
UniProtKB: Q9ULW6³
Ensembl: ENSG00000186462⁷

Search outside databases for aliases for NAP1L2 gene

Previous GC identifers: GC0XM068245 GC0XM069606 GC0XM070646 GC0XM071298 GC0XM072215

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for NAP1L2:

This gene encodes a member of the nucleosome assembly protein (NAP) family. The function of this

family member is unknown; however, mouse studies suggest that it represents a class of

tissue-specific factors interacting with chromatin to regulate neuronal cell proliferation.

[provided by RefSeq]

UniProtKB/Swiss-Prot: NP1L2_HUMAN, Q9ULW6

Function: Acidic protein which may be involved in interactions with other proteins or DNA (By

similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the NAP1L2 gene

Entrez Gene cytogenetic band: Xq13 Ensembl cytogenetic band: Xq13.2 HGNC cytogenetic band: Xq13

NAP1L2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X GeneLoc Exon Structure

GeneLoc location for GC0XM072348: (about GC identifiers)

Start:	72,348,860 bp from pter
End:	72,351,409 bp from pter
Size:	2,550 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000023.9 NT_011669.16

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: NP1L2_HUMAN, Q9ULW6 (See protein sequence)

Recommended Name: Nucleosome assembly protein 1-like 2

Size: 460 amino acids; 52542 Da

Subcellular location: Nucleus (Probable)

Secondary accessions: Q8TAN6

Post-translational modifications:

View phosphorylation sites using PhosphoSite²

REFSEQ proteins: NP_068798.1

ENSEMBL proteins:

ENSP00000362616

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (NAP1L2)
	Human Recombinant Proteins from Abnova (NAP1L2)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	TAS	8789438
GO:0005678	chromatin assembly complex	TAS	8789438

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Antibodies for NAP1L2:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for NAP1L2
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (NAP1L2), each with their Abpromise^SM
	Monoclonal and Polyclonal Antibodies from Abnova (NAP1L2)
	Novus Biologicals Antibodies for NAP1L2

Assays for NAP1L2:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q9ULW6

ProtoNet protein and cluster: Q9ULW6

1 Blocks protein family: IPB002164 Nucleosome assembly protein (NAP)

UniProtKB/Swiss-Prot: NP1L2_HUMAN, Q9ULW6

Similarity: Belongs to the nucleosome assembly protein (NAP) family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (NAP1L2)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_021963

Applied Biosystems Silencer® siRNAs for NAP1L2

Sigma-Aldrich siRNA for NAP1L2

Sigma-Aldrich shRNA for NAP1L2

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for NAP1L2
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_021963
Myc/DDK tagged cDNA clone in CMV expression vector: NM_021963
untagged cDNA clone in CMV expression vector: NM_021963

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_021963

UniProtKB/Swiss-Prot: NP1L2_HUMAN, Q9ULW6

Function: Acidic protein which may be involved in interactions with other proteins or DNA (By

nucleosome assembly protein 1-like 2,murine Bpx homolog

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Interacting protein for NAP1L2 (Q9ULW6²) via UniProtKB, MINT, and/or STRING
Interactant Interaction Details
GeneCard External ID(s)
-- Q9WMX2² MINT-7048724 MINT-7050993 MINT-7050403
About this table
1 Gene Ontology (GO) biological process term (links to tree view):
GO ID Qualified GO term Evidence PubMed IDs
GO:0006334 nucleosome assembly TAS 8789438
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for NAP1L2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (NAP1L2)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_021963

Sigma-Aldrich siRNA for NAP1L2

Sigma-Aldrich shRNA for NAP1L2

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_021963

REFSEQ mRNAs for NAP1L2 gene:

NM_021963.2

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_021963

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_021963
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_021963
                                 untagged cDNA clone in CMV expression vector: NM_021963

Additional cDNA sequence:

AB027013.1 AF136178.1 AK291297.1 AK316569.1 BC026325.1 CR457095.1

2 DOTS entries:

DT.309076 DT.97839188

24/45 AceView cDNA sequences (see all 45 ):

BQ721589 AF136178 AI205064 CA771292 AA634151 Z41332 AB027013 AA156109

AI350825 AI823491 AA081879 BF448963 BM725977 NM_021963 F08328 CA947068

CK826447 CD678482 BM683236 CK826446 BM719302 BC026325 CB242638 CA946795

highest scoring ESTs for NAP1L2:

AA081879 AA310738 AA322852 AA324132 AA330450 AB027013 AF136178 AI244044 AI694972 AI823491

Unigene Clusters for NAP1L2:

Nucleosome assembly protein 1-like 2

Hs.66180 [show with all ESTs], Hs.719281 [show with all ESTs]

Unigene Representative Sequences: AK303676, NM_021963

GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:

ENST00000373517

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

NAP1L2 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for NAP1L2

¹ / ² / ³

3 probe-sets matching NAP1L2 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
62533_at²^{, 3}	U95-C	1	1.00	1.00	1.00	1.00	AI823491	0.80	1.00	0.91	1
219368_at²^{, 3}	U133-A	1	1.00	1.00	--	--	NM_021963	0.60	1.00	0.82	1
219368_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

GeneDecks NAP1L2 for binary patterns associated with the probe-sets selected above

About GeneDecksing

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene clusters: Hs.66180 Hs.719281

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for NAP1L2 gene from 4 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	NAP1L2¹	--	nucleosome assembly protein 1-like 2	89.67(n) 85.37(a)	491959 XM_549079.2 XP_549079.2
chimpanzee (Pan troglodytes)	NAP1L2¹	--	nucleosome assembly protein 1-like 2	99.71(n) 99.57(a)	473666 XM_001141012.1 XP_001141012.1
rat (Rattus norvegicus)	Nap1l2¹	--	nucleosome assembly protein 1-like 2	85.04(n) 82.79(a)	317247 NM_001024789.1 NP_001019960.1
mouse (Mus musculus)	Nap1l2^{1, 5}	X (42.50 cM)⁵	nucleosome assembly protein 1-like 2^{1, 5}	84.86(n)¹ 83.84(a)¹	17954¹ NM_008671.1¹ NP_032697.1¹ AJ421480⁵ AK051439⁵ (see all 11)

About this table Species with no ortholog for NAP1L2

ENSEMBL Gene Tree for NAP1L2

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for NAP1L2 gene

NAP1L6² NAP1L3² NAP1L5² NAP1L4² NAP1L1²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/18 NCBI SNPs in NAP1L2 are shown (see all 18 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 16)

AB	SNP ID	Valid	Chr X pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs2885665^1,2	A,C,F,H,O	72352073(-)	`TTACTT/AGTCAC`	1	--	ng3¹ tfbs³		17		NS EU EA WA NA	1098
	rs5938504^1,2	C,F,H	72351535(+)	`AGATCT/CATCAA`	1	--	ng3¹ tfbs³		8		EU EA WA	616
	rs12689678^1,2	H	72351353(+)	`CTCCGC/GAAGCA`	1	--	ut5¹ ese³		4		EU EA WA	420
	rs15738^1,2	C	72348953(+)	`ACTTTC/TAATGA`	1	--	ut3¹		0	--	--	--	--
	rs2362986^1,2	H	72349713(-)	`GCTTGT/ATGCAA`	1	X/C	non¹		3		EU EA	286
	rs5982097^1,2	C,F,H	72350508(+)	`TCTTCA/GTGACA`	1	H/H	syn¹		4		EU EA WA	418
	rs3747405^1,2	H	72350136(+)	`AGCACA/GTAGGT`	1	Y/Y	syn¹ ese³		4		EU EA WA	418
	rs34495238^1,2	F	72351027(-)	`AAGGAG/ACTGTC`	1	E/E	syn¹		1		NA	72
--	rs35199885^1,2	--	72350210(+)	`GAGGG-/GCTCGC`	1	P/	fra¹		0	--	--	--	--
--	rs35676900^1,2	--	72348574(+)	`TTCCC-/CTGCAA`	1	--	ng5¹		0	--	--	--	--

About this table

HapMap Linkage Disequilibrium images for NAP1L2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 300026

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
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6 PubMed articles for NAP1L2 gene:

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Cloning and characterization of a murine brain specific gene Bpx and its human homologue lying within the Xic candidate region. (PubMed id 8789438)^{2, 3} Rougeulle C. and Avner P. (1996)
Control of neurulation by the nucleosome assembly protein-1-like 2. (PubMed id 10932189)^{1, 3} Rogner U.C....Avner P. (2000)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )⁴ Aoki H.... Hino O. (2000)
Characterization of a highly complex region in Xq13 and mapping of three isodicentric breakpoints associated with preleukemia. (PubMed id 10756090)¹ McDonell N.... Chelly J. (2000)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 4674	HGNC: 7638	AceView: NAP1L2	Ensembl:ENSG00000186462	euGenes: HUgn4674
ECgene: NAP1L2	H-InvDB: NAP1L2

(According to HUGE)
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(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for NAP1L2
(Available from WIS Yeda, Salk, Tufts)

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(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for NAP1L2:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for NAP1L2-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for NAP1L2		Antibodies for NAP1L2
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for NAP1L2		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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NAP1L2 Gene

protein-coding GIFtS: 50

Aliases &
Descriptions
for NAP1L2

Summaries
for NAP1L2

Genomic Location
for NAP1L2

Proteins
for NAP1L2

Protein Domains/
Families
for NAP1L2

Gene Function
for NAP1L2

Pathways & Interactions
for NAP1L2

Drugs & Compounds
for NAP1L2

Transcripts
for NAP1L2

Expression
for NAP1L2

Orthologs
for NAP1L2

Paralogs
for NAP1L2

SNPs/Variants
for NAP1L2

Disorders & Mutations
for NAP1L2

Medical News
for NAP1L2

Publications
for NAP1L2

Search for NAP1L2

Genome Databases showing NAP1L2

Other Databases showing NAP1L2

Specialized Databases showing NAP1L2

Licensable Technologies
for NAP1L2

Services
for NAP1L2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

NAP1L2 Gene

protein-coding GIFtS: 50

Aliases & Descriptionsfor NAP1L2

Summariesfor NAP1L2

Genomic Locationfor NAP1L2

Proteinsfor NAP1L2

Protein Domains/Familiesfor NAP1L2

Gene Functionfor NAP1L2

Pathways & Interactionsfor NAP1L2

Drugs & Compoundsfor NAP1L2

Transcriptsfor NAP1L2

Expression for NAP1L2

Orthologsfor NAP1L2

Paralogsfor NAP1L2

SNPs/Variantsfor NAP1L2

Disorders & Mutationsfor NAP1L2

Medical Newsfor NAP1L2

Publicationsfor NAP1L2

Search for NAP1L2

Genome Databases showing NAP1L2

Other Databases showing NAP1L2

Specialized Databases showing NAP1L2

Licensable Technologiesfor NAP1L2

Servicesfor NAP1L2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for NAP1L2

Summaries
for NAP1L2

Genomic Location
for NAP1L2

Proteins
for NAP1L2

Protein Domains/
Families
for NAP1L2

Gene Function
for NAP1L2

Pathways & Interactions
for NAP1L2

Drugs & Compounds
for NAP1L2

Transcripts
for NAP1L2

Expression
for NAP1L2

Orthologs
for NAP1L2

Paralogs
for NAP1L2

SNPs/Variants
for NAP1L2

Disorders & Mutations
for NAP1L2

Medical News
for NAP1L2

Publications
for NAP1L2

Licensable Technologies
for NAP1L2

Services
for NAP1L2