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NANP Gene

protein-coding   GIFtS: 49

GC20M025543
N-acetylneuraminic acid phosphatase
(Previous names: chromosome 20 open reading frame 147, haloacid dehalogenase-like hydrolase domain containing 4 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbols: C20orf147, HDHD4)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
C20orf147 2, 3
EC 3.1.3.29 3
HDHD4 2, 3, 5
MGC26833 1, 2
N-acylneuraminate-9-phosphatase 2
Neu5Ac-9-Pase 2, 3
OTTHUMP00000030503 2
dJ694B14.3 1, 2
Descriptions
Haloacid dehalogenase-like hydrolase domain-containing
protein 4 3
N-acetylneuraminic acid phosphatase 2
chromosome 20 open reading frame 147 1
haloacid dehalogenase-like hydrolase domain containing 4 1, 2
External Ids
HGNC: 161401
Entrez Gene: 1408382
UniProtKB: Q8TBE93
Ensembl: ENSG000001701917
Search outside databases for aliases for NANP gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

  --
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NANP gene  

Entrez Gene cytogenetic band: 20p11.1   Ensembl cytogenetic band:  20p11.21   HGNC cytogenetic band: 20p11.1

NANP Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20M025543:     (about GC identifiers)

Start:
25,541,575 bp from pter
End:
25,552,621 bp from pter
Size:
11,047 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000020.9  NT_011387.8  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: NANP_HUMAN, Q8TBE9 (See protein sequence)
Recommended Name: N-acylneuraminate-9-phosphatase  
Size: 248 amino acids; 27813 Da
Cofactor: Magnesium
Sequence caution: Sequence=BAB85055.1; Type=Miscellaneous discrepancy; Note=It seems to be derived
from genomic DNA and not from cDNA;
PDB structures from and Proteopedia :
2W4M (3D)    
Secondary accessions: Q5JYN8 Q8TE97 Q9Y3N0

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_689880.1  

    ENSEMBL proteins: 
    ENSP00000302441 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (NANP)
    Human Recombinant Proteins from Abnova (NANP)
                  OriGene Purified Recombinant Human Protein: NANP 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005575 cellular_component ND--
    About this table

    Antibodies for NANP: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for NANP
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (NANP), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (NANP)
    Novus Biologicals Antibodies for NANP

    Assays for NANP: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR005833 Haloacid_DH/epoxide_hydro
     IPR011950 HAD-SF_hydro_IA_CTE7
     IPR006439 HAD-SF_hydro_IA_v1
     IPR005834 Dehalogen-like_hydro


       GeneDecks  NANP for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q8TBE9

    ProtoNet protein and cluster: Q8TBE9

    1 Blocks protein family: IPB005833 Haloacid dehalogenase/epoxide hydrolase family signature

    UniProtKB/Swiss-Prot: NANP_HUMAN, Q8TBE9
    Similarity: Belongs to the HAD-like hydrolase superfamily. NANP family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NANP)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_152667

                  Applied Biosystems Silencer® siRNAs for NANP

                  Sigma-Aldrich siRNA for NANP  
                         Sigma-Aldrich shRNA for NANP  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for NANP
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_152667
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_152667
                                     untagged cDNA clone in CMV expression vector: NM_152667 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_152667

    UniProtKB/Swiss-Prot: NANP_HUMAN, Q8TBE9
    Catalytic activity: N-acylneuraminate 9-phosphate + H(2)O = N-acylneuraminate + phosphate
    Enzyme regulation: Inhibited by vanadate and calcium
    Biophysicochemical properties: Kinetic parameters: KM=0.09 mM for N-acetylneuraminate 9-P; KM=19.2
    mM for fructose 1,6-P2; KM=2.7 mM for 6-P-gluconate; KM=5.9 mM for N-acetylglucosamine 6-P;
    Vmax=112 umol/min/mg enzyme with N-acetylneuraminate 9-P as substrate; Vmax=6.10 umol/min/mg
    enzyme with fructose 1,6-P2 as substrate; Vmax=2.79 umol/min/mg enzyme with 6-P-gluconate as
    substrate; Vmax=2.46 umol/min/mg enzyme with N-acetylglucosamine 6-P as substrate; Vmax=0.149
    umol/min/mg enzyme with sorbitol 6-P as substrate; Vmax=0.140 umol/min/mg enzyme with 3-P
    glycerate as substrate; Vmax=0.095 umol/min/mg enzyme with P-serine as substrate; Vmax=0.094
    umol/min/mg enzyme with glycerol 3-P as substrate; Vmax=0.090 umol/min/mg enzyme with ribulose 5-P
    as substrate; Vmax=0.072 umol/min/mg enzyme with N-acetylmannosamine 6-P as substrate; Vmax=0.063
    umol/min/mg enzyme with arabinose 6-P as substrate; Vmax=0.042 umol/min/mg enzyme with ribose 6-P
    as substrate; Vmax=0.023 umol/min/mg enzyme with glucosamine 6-P as substrate; Vmax=0.019
    umol/min/mg enzyme with phosphoglycolate as substrate; Vmax=0.019 umol/min/mg enzyme with fructose
    6-P as substrate; Vmax=0.017 umol/min/mg enzyme with glycerol 2-P as substrate; Vmax=0.016
    umol/min/mg enzyme with glucose 6-P as substrate; Vmax=0.015 umol/min/mg enzyme with mannose 6-P
    as substrate; Vmax=0.004 umol/min/mg enzyme with glucose 1-P as substrate; Vmax=0.002 umol/min/mg
    enzyme with P-enolpyruvate as substrate; Vmax=0.001 umol/min/mg enzyme with ATP as substrate;
    Enzyme Number (IUBMB): EC 3.1.3.29 

    1 MGI phenotypic allele for Nanp (no phenotypes)


    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000287 magnesium ion binding IEA--
    GO:0008967 phosphoglycolate phosphatase activity IEA--
    GO:0016787 hydrolase activity IEA--
    GO:0050124 N-acylneuraminate-9-phosphatase activity IDA16237198
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  NANP  (Your Favorite Gene powered by Ingenuity) 
     Aminosugars Metabolism

       GeneDecks  NANP for the pathways selected above  
    About GeneDecksing

    1 Kegg Pathway  (Kegg details for NANP):
     hsa00530 Aminosugars metabolism

       GeneDecks  NANP for the pathways selected above  
    About GeneDecksing
    UniProtKB/Swiss-Prot: NANP_HUMAN, Q8TBE9
    Pathway: Amino-sugar metabolism; N-acetylneuraminic acid biosynthesis

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005975 carbohydrate metabolic process IEA--
    GO:0008152 metabolic process IEA--
    GO:0046380 N-acetylneuraminate biosynthetic process IDA16237198
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for NANP

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NANP)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_152667

                  Sigma-Aldrich siRNA for NANP  
                         Sigma-Aldrich shRNA for NANP  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_152667  

    REFSEQ mRNAs for NANP gene: 

    NM_152667.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_152667  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_152667
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_152667
                                     untagged cDNA clone in CMV expression vector: NM_152667 

    Additional cDNA sequence: 

    AK055472.1 AL833116.1 BC003415.1 BC022552.1 

    2 DOTS entries:

    DT.40297535  DT.100776215 

    24/51 AceView cDNA sequences (see all 51 ):

    AI150963 AI422507 AI129166 BQ937679 BX109319 CF126993 BF475385 AI130756 
    AW452991 AI348660 BE045534 AI280765 BU153984 BP351640 BQ224337 AK055472 
    BX404520 BM801309 AI693731 BI087780 BF511870 AL833116 AI146450 BG705027 

    highest scoring ESTs for NANP:

    AI146450 AK055472 AK074335 AL833116 BC022552 BG705027 BQ224337 BQ937679 BU153984 BX404146 

    Unigene Cluster for NANP:

    N-acetylneuraminic acid phosphatase
    Hs.143137  [show with all ESTs]
    Unigene Representative Sequence: AL833116


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000304788  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    NANP expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for NANP

    1 / 2 / 3

    4 probe-sets matching NANP gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    77008_at2, 3 U95-E 1 1.00 1.00 0.67 1.09 AI146450 0.80 1.00 0.91 1

    60323_at2, 3 U95-C 1 1.00 1.00 0.48 0.91 AI693731 0.60 1.00 0.82 1

    228073_at2, 3 U133-B 1 1.00 1.00 -- -- AI146450 0.80 1.00 0.91 1

    228073_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: --

    SOURCE GeneReport for Unigene cluster: Hs.143137
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for NANP gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    LOC6081821   -- similar to haloacid dehalogenase-like hydrolase domain more 88.84(n)
    91.13(a)
    608182  XM_845120.1  XP_850213.1 
    chimpanzee
    (Pan troglodytes)
    NANP1   -- N-acetylneuraminic acid phosphatase 99.29(n)
    99.29(a)
    745164  XM_001150653.1  XP_001150653.1 
    cow
    (Bos taurus)
    NANP1   -- N-acetylneuraminic acid phosphatase 88.71(n)
    94.35(a)
    516539  XM_594694.3  XP_594694.2 
    rat
    (Rattus norvegicus)
    Nanp1   -- N-acetylneuraminic acid phosphatase 86.91(n)
    94.74(a)
    311530  NM_001009409.1  NP_001009409.1 
    mouse
    (Mus musculus)
    Nanp1, 5 25
    N-acetylneuraminic acid phosphatase1, 5 86.5(n)1
    94.33(a)1
    673111  NM_026086.11  NP_080362.11 
     AK0055665  AK0213445  (see all 9)
    About this table        Species with no ortholog for NANP

    ENSEMBL Gene Tree for NANP
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/70 NCBI SNPs in NANP are shown (see all 70 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 59)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 20 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs9580751,2
    A,C,F,H,O25543123(-) GGAACC/TGGAGA 1 -- ut31 ese317Minor allele frequency- T:0.30NA EA NS MN EU WA 1300
    --
    rs60371581,2
    A,C,F,H25543466(+) aaaaaA/Cacaca 1 -- ut31 ese35Minor allele frequency- C:0.12EA NA 316
    rs60507241,2
    A,C,F,H25554327(+) ACCCAT/CAGGCA 1 -- ng317Minor allele frequency- C:0.18EU EA WA NA 552
    rs60371661,2
    A,C,F,H25554082(+) gtaagC/Tgagac 1 -- ng318Minor allele frequency- T:0.18EU EA WA 838
    rs37522731,2
    A,C,F,H25542783(+) CACGAA/TAGGGC 1 -- ng5114Minor allele frequency- T:0.27MN EU EA WA NA 1340
    rs60507111,2
    A,C,F,H25544519(+) CTCAGC/TAAATT 1 -- ut314Minor allele frequency- T:0.02EU NA 262
    rs48154381,2
    A,C,F,H25554369(+) ACCTCA/GTTGAC 1 -- ng3112Minor allele frequency- N:0.00EU EA WA NA 1052
    --
    rs60507101,2
    A,C,F,H25544312(+) TTCCTT/CAAATT 1 -- ut31 ese34Minor allele frequency- C:0.18EU EA WA 412
    rs81200651,2
    A,F,H25552655(+) CCCAGA/CGAGAC 1 -- ng31 ese34Minor allele frequency- C:0.17EU EA WA 412
    rs60371641,2
    C,F,H25553550(+) ATTACC/TTGATT 1 -- ng317Minor allele frequency- T:0.17EU EA WA NA 562
    About this table

    HapMap Linkage Disequilibrium images for NANP (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 610763

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    7 PubMed articles for NANP gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 140838 HGNC: 16140 AceView: HDHD4 Ensembl:ENSG00000170191 euGenes: HUgn140838
    ECgene: NANP H-InvDB: NANP
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for NANP:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for NANP-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for NANP Antibodies for NANP
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for NANP Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant & Natural Proteins  

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     Recombinant Proteins
    (NANP)
     Antibodies (NANP)
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for NANP




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for NANP

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