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NANOS1 Gene

protein-coding   GIFtS: 51

GC10P120779
nanos homolog 1 (Drosophila)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC_Rep1a 3
NOS-1 3
NOS1 1, 2, 3, 5
OTTHUMP00000046853 2
Descriptions
nanos homolog 1 2
nanos homolog 1 (Drosophila) 2
External Ids
HGNC: 230441
Entrez Gene: 3407192
UniProtKB: Q8WY413
Ensembl: ENSG000001886137
Search outside databases for aliases for NANOS1 gene

Previous GC identifers: GC10U900443 GC10P120453

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: NANO1_HUMAN, Q8WY41
Function: May regulate translation of specific mRNAs by forming a complex with PUM2 that associates
with the 3'-UTR of mRNA targets

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NANOS1 gene  

Entrez Gene cytogenetic band: 10q26.11   Ensembl cytogenetic band:  10q26.11   HGNC cytogenetic band: 10q26.13

NANOS1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10P120779:     (about GC identifiers)

Start:
120,779,218 bp from pter
End:
120,783,844 bp from pter
Size:
4,627 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000010.9  NT_030059.12  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: NANO1_HUMAN, Q8WY41 (See protein sequence)
Recommended Name: Nanos homolog 1  
Size: 292 amino acids; 30230 Da
Subunit: Interacts with PUM2
Subcellular location: Cytoplasm, perinuclear region

REFSEQ proteins: NP_955631.1  

ENSEMBL proteins: 
ENSP00000345924 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (Nanos Homologue 1 (NANOS1))
Browse Abnova for Human Recombinant Proteins
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005737 cytoplasm IDA12690449
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Antibodies for NANOS1: 
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Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for NANOS1
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Antibodies from Abcam (Nanos Homologue 1 (NANOS1)), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (NANOS1)
Novus Biologicals Antibody for NANOS1

Assays for NANOS1: 
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Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR008705 Znf_Nanos


   GeneDecks  NANOS1 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q8WY41

ProtoNet protein and cluster: Q8WY41

1 Blocks protein family: IPB008705 Nanos RNA-binding

UniProtKB/Swiss-Prot: NANO1_HUMAN, Q8WY41
Domain: The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule
of zinc. It is essential for the translation repression activity of the protein (By similarity)
Similarity: Belongs to the nanos family
Similarity: Contains 1 nanos-type zinc finger

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NANOS1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (NANOS1)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001009553

              Applied Biosystems Silencer® siRNAs for NANOS1

              Sigma-Aldrich siRNA for NANOS1  
                     Sigma-Aldrich shRNA for NANOS1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_199461
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_199461
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001009553 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_199461

UniProtKB/Swiss-Prot: NANO1_HUMAN, Q8WY41
Function: May regulate translation of specific mRNAs by forming a complex with PUM2 that associates
with the 3'-UTR of mRNA targets

1 MGI mutant phenotype (inferred from 1 allele(MGI details for Nanos1):

normal

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0003723 RNA binding IEA--
GO:0008270 zinc ion binding IEA--
GO:0046872 metal ion binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


 Gene Network CentralTM Interacting Genes and Proteins Network for  NANOS1 


5/19 Interacting proteins for NANOS1 (ENSP000003459243) via UniProtKB, MINT, and/or STRING (see all 19 )
InteractantInteraction Details
GeneCardExternal ID(s)
PUM2ENSP000003543703STRING (score=.958)
SNAPINENSP000003576743STRING (score=.925)
CTNND1ENSP000003548233STRING (score=.906)
CALM3ENSP000002912953STRING (score=.897)
KRIT1ENSP000003446683STRING (score=.88)
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1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006417 regulation of translation IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for NANOS1
1 Novoseek chemical compound relationship for NANOS1 gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
nitric oxide 35.95 1 8972893 (1)
About this table


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NANOS1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (NANOS1)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001009553

              Sigma-Aldrich siRNA for NANOS1  
                     Sigma-Aldrich shRNA for NANOS1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_199461  

REFSEQ mRNAs for NANOS1 gene: 

NM_199461.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_199461  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_199461
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_199461
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001009553 

Additional cDNA sequence: 

AF086393.1 AF275269.2 AF458985.1 

4 DOTS entries:

DT.419410  DT.100798858  DT.40302531  DT.92421843 

24/66 AceView cDNA sequences (see all 66 ):

AI138906 AW340709 AI422817 AI572316 AI421274 BE467063 AI371014 AI090689 
BX094286 AW134982 W79223 AW026179 AI273269 N30160 AF458985 AA989054 
BE503548 AI188525 BF590809 BE218421 W07677 AW137865 NM_199461 BE467767 

highest scoring ESTs for NANOS1:

AA459718 AA461301 AA482971 AA832519 AA989054 AF086393 AF275269 AF458985 AI090689 AI138906 

Unigene Cluster for NANOS1:

Nanos homolog 1 (Drosophila)
Hs.591918  [show with all ESTs]
Unigene Representative Sequence: NM_199461


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000340087  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

NANOS1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for NANOS1

1 / 2 / 3

5 probe-sets matching NANOS1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

83101_at2, 3 U95-D 1 1.00 1.00 0.86 0.72 AI419908 0.80 0.88 0.84 1

59795_r_at2, 3 U95-B 1 1.00 1.00 0.66 0.71 N30160 0.80 0.88 0.84 1
59792_i_at2, 3 U95-B 1 1.00 1.00 0.67 1.63 N30160 0.80 0.88 0.84 1

228523_at2, 3 U133-B 1 1.00 1.00 -- -- AW970089 0.60 0.83 0.73 1

228523_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  NANOS1 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.591918

Expression variation in blood from EXPOLDB for NANOS1

UniProtKB/Swiss-Prot: NANO1_HUMAN, Q8WY41
Tissue specificity: Predominantly expressed in testis. Specifically expressed during germline
development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the
germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage
germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic
oocytes, suggesting that it may be expressed in premeiotic female germ cells

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for NANOS1 gene from 5 species
Organism Gene Locus Description Human
Similarity
NCBI accessions
chimpanzee
(Pan troglodytes)
NANOS11   -- nanos homolog 1 (Drosophila) 99.24(n)
99.09(a)
450773  XM_508067.2  XP_508067.2 
rat
(Rattus norvegicus)
Nanos11   -- nanos homolog 1 (Drosophila) 86.19(n)
85.55(a)
365494  XM_345053.3  XP_345054.2 
mouse
(Mus musculus)
Nanos11, 5 195
nanos homolog 1 (Drosophila)1, 5 85.91(n)1
85.28(a)1
3323971  NM_178421.31  NP_848508.21 
 AB0950295  AK0429895  (see all 10)
African clawed frog
(Xenopus laevis)
Xcat-22   -- X.laevis Xcat-2 mRNA 77.36(n)  X72340.1 
tropical clawed frog
(Silurana tropicalis)
Xcat-22   -- Xcat-2 protein 79.41(n)  AF256086.1 
About this table        Species with no ortholog for NANOS1

ENSEMBL Gene Tree for NANOS1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for NANOS1 gene
NANOS32  NANOS22  

Pseudogenes
2 related pseudogenes


(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/36 NCBI SNPs in NANOS1 are shown (see all 36 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 38)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 10 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
--
rs5468081,2
A,C120782693(-) ttggaG/Tacaga 1 -- ut31 ese30--------
rs6144651,2
C,H120781050(+) TTGGCC/ATTTTC 1 -- ut317Minor allele frequency- A:0.00EA NA EU WA 470
rs15009861,2
A,C120778537(-) GGCTTC/TCGTTT 1 -- ng510--------
rs127831721,2
C,H120782108(+) ATCAGC/TGCATT 1 -- ut31 ese34Minor allele frequency- T:0.00EU EA WA 418
rs10613661,2
C120783998(-) AAGGAC/TTTTTC 1 -- ng310--------
--
rs127742971,2
--120778982(+) GCCCCC/TTCCCG 1 -- ng510--------
--
rs32159531,2
--120781769(-) TATCG-/GG    
   TATTT
ACCCC
1 -- ut310--------
--
rs681420301,2
--120783380(+) AAAAA-/AAAAAA 1 -- ut310--------
--
rs741576181,2
--120781456(+) CTAACC/TCCTAC 1 -- ut310--------
--
rs127743111,2
--120779008(+) GACAGC/TTGGCG 1 -- ng51 tfbs30--------
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HapMap Linkage Disequilibrium images for NANOS1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 608226

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10 PubMed articles for NANOS1 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 340719 HGNC: 23044 AceView: NANOS1 Ensembl:ENSG00000188613 euGenes: HUgn340719
ECgene: NANOS1 H-InvDB: NANOS1
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for NANOS1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Predesigned and custom siRNAs for NANOS1 Antibodies for NANOS1
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for NANOS1 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

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 Recombinant Proteins
(Nanos Homologue 1 (NANOS1))
 Antibodies (Nanos Homologue 1 (NANOS1))
 Search OriGene for NANOS1
 Search Tocris compounds for NANOS1




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for NANOS1

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