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NALCN Gene

protein-coding   GIFtS: 44

GC13M100505
sodium leak channel, non-selective
(Previous name: voltage gated channel like 1 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: VGCNL1)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
CanIon 1, 2, 3
FLJ23913 2
FLJ44659 2
FLJ44764 2
MGC74524 2
OTTHUMP00000018654 2
OTTHUMP00000018655 2
OTTHUMP00000018656 2
OTTHUMP00000040730 2
VGCNL1 2, 3
bA430M15.1 1, 2
Descriptions
Voltage gated channel-like protein 1 3
four repeat voltage-gated ion channel 2
sodium leak channel, non-selective 2
voltage gated channel like 1 1, 2
External Ids
HGNC: 190821
Entrez Gene: 2592322
UniProtKB: Q8IZF03
Ensembl: ENSG000001024527
Search outside databases for aliases for NALCN gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for NALCN:
NALCN forms a voltage-independent, nonselective, noninactivating cation channel permeable to Na+,
K+, and Ca(2+). It is responsible for the neuronal background sodium leak conductance (Lu et al.,
2007 [PubMed 17448995]).[supplied by OMIM]

UniProtKB/Swiss-Prot: NALCN_HUMAN, Q8IZF0
Function: Voltage-independent, cation-nonselective channel which is permeable to sodium, potassium
and calcium ions. Responsible for the background sodium ion leak current in neurons and controls
neuronal excitability. Activated either by neuropeptides substance P or neurotensin. Required for
normal respiratory rhythm and neonatal survival (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 13q32.3   Ensembl cytogenetic band:  13q33.1   HGNC cytogenetic band: 13q32.3

NALCN Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 13         GeneLoc Exon Structure

GeneLoc location for GC13M100505:     (about GC identifiers)

Start:
100,504,131 bp from pter
End:
100,866,814 bp from pter
Size:
362,684 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000013.9  NT_009952.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: NALCN_HUMAN, Q8IZF0 (See protein sequence)
Recommended Name: Sodium leak channel non-selective protein  
Size: 1738 amino acids; 200331 Da
Subunit: Interacts with UNC79/KIAA1409 and UNC80 (By similarity)
Subcellular location: Membrane; Multi-pass membrane protein (Potential)
Secondary accessions: Q6P2S6 Q6ZMI7 Q8IZZ1 Q8TAH1
Alternative splicing: 3 isoforms:  Q8IZF0-1   Q8IZF0-2   Q8IZF0-3   

Post-translational modifications:

  • Phosphorylated on tyrosine residues (By similarity)1


  • REFSEQ proteins: NP_443099.1  

    ENSEMBL proteins: 
    ENSP00000365367 ENSP00000365373 ENSP00000251127 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (VGCNL1)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0016020 membrane IEA--
    GO:0016021 integral to membrane IEA--
    About this table

    Antibodies for NALCN: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (VGCNL1), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for NALCN

    Assays for NALCN: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR005821 Ion_trans


       GeneDecks  NALCN for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q8IZF0

    ProtoNet protein and cluster: Q8IZF0

    UniProtKB/Swiss-Prot: NALCN_HUMAN, Q8IZF0
    Domain: Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2,
    S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent
    the voltage-sensor and are characterized by a series of positively charged amino acids at every
    third position
    Similarity: Belongs to the cation-nonselective channel family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NALCN)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (VGCNL1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_052867

                  Applied Biosystems Silencer® siRNAs for NALCN

                  Sigma-Aldrich siRNA and siRNA Panels for NALCN  
                         Sigma-Aldrich shRNA Panels and shRNA for NALCN  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for NALCN
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_052867
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_052867
                                     untagged cDNA clones in CMV expression vector (see all 4): NM_052867 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_052867

    UniProtKB/Swiss-Prot: NALCN_HUMAN, Q8IZF0
    Function: Voltage-independent, cation-nonselective channel which is permeable to sodium, potassium
    and calcium ions. Responsible for the background sodium ion leak current in neurons and controls
    neuronal excitability. Activated either by neuropeptides substance P or neurotensin. Required for
    normal respiratory rhythm and neonatal survival (By similarity)

    7 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Nalcn):

    behavior/neurologicalembryogenesishomeostasis/metabolismlethality-prenatal/perinatalnervous system
    no phenotypic analysisrespiratory system

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005244 voltage-gated ion channel activity IEA--
    GO:0005272 sodium channel activity IEA--
    GO:0031402 sodium ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


      --

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006811 ion transport IEA--
    GO:0006814 sodium ion transport IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for NALCN

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NALCN)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (VGCNL1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_052867

                  Sigma-Aldrich siRNA and siRNA Panels for NALCN  
                         Sigma-Aldrich shRNA Panels and shRNA for NALCN  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_052867  

    REFSEQ mRNAs for NALCN gene: 

    NM_052867.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_052867  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_052867
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_052867
                                     untagged cDNA clones in CMV expression vector (see all 4): NM_052867 

    Additional cDNA sequence: 

    AF339780.1 AK002089.1 AK055506.1 AK094390.1 AK094669.1 AK126621.1 AK126718.1 AK172752.1 
    AY141972.1 BC012128.1 BC028390.1 BC064343.1 

    6 DOTS entries:

    DT.109049  DT.100009034  DT.91697284  DT.91655058  DT.40115258  DT.86856304 

    24/72 AceView cDNA sequences (see all 72 ):

    AW149508 BU948062 AI168806 BC064343 AI811060 CA775297 AU145670 AY141972 
    BM556576 NM_052867 AK002089 BX508225 BC012128 BU069355 BE220114 AU138314 
    AK126621 AK126718 AW205016 BE671149 AU119288 N40916 BE327303 AK094669 

    Unigene Cluster for NALCN:

    Sodium leak channel, non-selective
    Hs.525146  [show with all ESTs]
    Unigene Representative Sequence: NM_052867


    GeneLoc Exon Structure

    3 Ensembl transcripts including schematic representations:
    ENST00000376196  ENST00000376200  ENST00000251127  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    NALCN expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for NALCN

    1 / 2

    6 probe-sets matching NALCN gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    51064_at2 U95-B 1 1.00 1.00 1.00 1.00 -- -- -- -- --

    228608_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

    242880_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

    228608_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1554702_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    242880_at2 U133Plus2 1 0.82 1.00 -- -- -- -- -- -- --
    GeneDecks  NALCN for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AGCTCAGGAG

    SOURCE GeneReport for Unigene cluster: Hs.525146
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for NALCN gene from 5/16 species (see all 16 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    NALCN1   -- sodium leak channel, non-selective 91.6(n)
    99.19(a)
    485535  XM_542654.2  XP_542654.2 
    chimpanzee
    (Pan troglodytes)
    NALCN1   -- sodium leak channel, non-selective 99.62(n)
    100(a)
    452643  XM_001149573.1  XP_001149573.1 
    cow
    (Bos taurus)
    NALCN1   -- sodium leak channel, non-selective 89.72(n)
    96.78(a)
    536103  XM_616223.3  XP_616223.3 
    rat
    (Rattus norvegicus)
    Nalcn1   -- sodium leak channel, non-selective 89.46(n)
    98.62(a)
    266760  NM_153630.1  NP_705894.1 
    mouse
    (Mus musculus)
    Nalcn1, 5 145
    sodium leak channel, non-selective1, 5 89.57(n)1
    98.68(a)1
    3383701  NM_177393.41  NP_796367.31 
     AC1027835  AC1248185  (see all 14)
    About this table        Species with no ortholog for NALCN

    ENSEMBL Gene Tree for NALCN
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/2588 NCBI SNPs in NALCN are shown (see all 2588 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 925)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 13 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs6827671,2
    A,C,F,H,O100504630(-) TTCCTC/TTGAAC 1 -- ut317Minor allele frequency- T:0.36NA EU EA WA 560
    rs6826661,2
    A,C,F,H,O100504702(-) TGCTAT/CGTTAG 1 -- ut31 ese311Minor allele frequency- C:0.36EU EA WA NA 976
    rs89221,2
    A,C,F,H,O100504194(-) TGATTT/GGATGT 1 -- ut3112Minor allele frequency- G:0.23MN NA EU EA WA 1204
    rs79899321,2
    A,C,F,H100504124(+) TCAGAG/ATAAAT 1 -- ng5111Minor allele frequency- A:0.02NA EU EA WA 980
    rs95575811,2
    A,C,F,H100504717(+) TGTGTT/CAGGAT 1 -- ut31 ese37Minor allele frequency- C:0.45NA EU EA WA 560
    rs95183951,2
    A,C,F,H100867076(+) CCCGCA/CGTCAG 1 -- ng314Minor allele frequency- C:0.50EU EA WA 412
    rs21523241,2
    C,F,H100868161(+) GCTTGA/TTCTAA 1 -- ng3111Minor allele frequency- T:0.13NA EA EU WA 1084
    rs37944121,2
    C,F100504417(+) CATCTG/AAACAG 1 -- ut31 ese33Minor allele frequency- A:0.02NA 142
    rs95856061,2
    F,H100503909(+) TTCTGT/CTCATT 1 -- ng514Minor allele frequency- C:0.07EU EA WA 418
    rs37944111,2
    C,F100504223(+) ACATGA/CCTGGT 1 -- ut314Minor allele frequency- C:0.12EA NA 1642
    About this table

    HapMap Linkage Disequilibrium images for NALCN (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 611549

    Human Genome Epidemiology Navigator: NALCN (1 document)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/12 PubMed articles for NALCN gene (see all 12 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 259232 HGNC: 19082 AceView: VGCNL1 Ensembl:ENSG00000102452 euGenes: HUgn259232
    ECgene: NALCN H-InvDB: NALCN
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for NALCN:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for NALCN-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for NALCN Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for NALCN Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant Proteins
    (VGCNL1)
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     Search OriGene for NALCN
     Search Tocris compounds for NALCN




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     Search Novus for antibodies for NALCN

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