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NAGK Gene

protein-coding   GIFtS: 59

GC02P071207
N-acetylglucosamine kinase
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 2.7.1.59 3
GNK 1, 2, 5
HSA242910 2
Descriptions
GlcNAc kinase 3
N-acetylglucosamine kinase 2, 3
External Ids
HGNC: 171741
Entrez Gene: 555772
UniProtKB: Q9UJ703
Ensembl: ENSG000001243577
Search outside databases for aliases for NAGK gene

Previous GC identifers: GC02P071305 GC02P071506 GC02P071270

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for NAGK:
N-acetylglucosamine kinase (NAGK; EC 2.7.1.59) converts endogenous N-acetylglucosamine (GlcNAc), a
major component of complex carbohydrates, from lysosomal degradation or nutritional sources into
GlcNAc 6-phosphate. NAGK belongs to the group of N-acetylhexosamine kinases and is a prominent
salvage enzyme of amino sugar metabolism in mammals.[supplied by OMIM]

UniProtKB/Swiss-Prot: NAGK_HUMAN, Q9UJ70
Function: Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex
carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has
ManNAc kinase activity (By similarity)

Gene Wiki entry for NAGK

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NAGK gene  

Entrez Gene cytogenetic band: 2p13.3   Ensembl cytogenetic band:  2p13.3   HGNC cytogenetic band: 2p24.3-p24.1

NAGK Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P071207:     (about GC identifiers)

Start:
71,148,955 bp from pter
End:
71,159,279 bp from pter
Size:
10,325 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000002.10  NT_022184.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: NAGK_HUMAN, Q9UJ70 (See protein sequence)
Recommended Name: N-acetyl-D-glucosamine kinase  
Size: 344 amino acids; 37376 Da
Subunit: Homodimer
PDB structure from and Proteopedia :
2CH5 (3D)    2CH6 (3D)    
Secondary accessions: Q53HD5 Q6IA84 Q9BS29 Q9BVP0 Q9NV37

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_060037.2  

    ENSEMBL proteins: 
    ENSP00000244204 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (N acetylglucosamine kinase )
    Human Recombinant Proteins from Abnova (NAGK)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005625 soluble fraction IEA--
    About this table

    Antibodies for NAGK: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for NAGK
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (N acetylglucosamine kinase ), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (NAGK)
    Novus Biologicals Antibody for GNK

    Assays for NAGK: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR002731 ATPase_BadF


       GeneDecks  NAGK for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9UJ70

    ProtoNet protein and cluster: Q9UJ70

    1 Blocks protein family: IPB002731 ATPase

    UniProtKB/Swiss-Prot: NAGK_HUMAN, Q9UJ70
    Similarity: Belongs to the eukaryotic-type N-acetylglucosamine kinase family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NAGK)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NAGK)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_017567

                  Applied Biosystems Silencer® siRNAs for NAGK

                  Sigma-Aldrich siRNA and siRNA Panels for NAGK  
                         Sigma-Aldrich shRNA Panels and shRNA for NAGK  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for NAGK
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_017567
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_017567
                                     untagged cDNA clones in CMV expression vector (see all 3): AK092876 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_017567

    UniProtKB/Swiss-Prot: NAGK_HUMAN, Q9UJ70
    Function: Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex
    carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has
    ManNAc kinase activity (By similarity)
    Catalytic activity: ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate
    Enzyme Number (IUBMB): EC 2.7.1.59 

    1 MGI mutant phenotype (inferred from 1 allele(MGI details for Nagk):

    normal

    5/7 Gene Ontology (GO) molecular function terms (links to tree view) (see all 7 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0005515 protein binding IPI15231747 16189514
    GO:0005524 ATP binding IEA--
    GO:0009384 N-acylmannosamine kinase activity IEA--
    GO:0016301 kinase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    2 Sigma-Aldrich "Your Favorite Gene" Pathways for  NAGK  (Your Favorite Gene powered by Ingenuity) 
     Glutamate Metabolism
     Aminosugars Metabolism

       GeneDecks  NAGK for the pathways selected above  
    About GeneDecksing

    1 Kegg Pathway  (Kegg details for NAGK):
     hsa00530 Aminosugars metabolism

       GeneDecks  NAGK for the pathways selected above  
    About GeneDecksing
     Gene Network CentralTM Interacting Genes and Proteins Network for  NAGK 


    5/17 Interacting proteins for NAGK (ENSP000002442043 Q9UJ701, 2) via UniProtKB, MINT, and/or STRING (see all 17 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    LNX1Q8TBB11, 2EBI-372578, EBI-739832 MINT-67311 EBI-372578, EBI-739832 MINT-67311
    DCP2Q8IU601EBI-372578, EBI-521577
    LNX1Q8TBB11, 2EBI-372578, EBI-739832 MINT-67311 EBI-372578, EBI-739832 MINT-67311
    CCDC158Q5M9N02MINT-67891
    NAGKQ9UJ702MINT-67601
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006044 N-acetylglucosamine metabolic process TAS10824116
    GO:0006051 N-acetylmannosamine metabolic process TAS10824116
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for NAGK
    8 Novoseek chemical compound relationships for NAGK gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    n-acetylglutamate 90.75 10 12875848 (2), 16122935 (1), 12351849 (1), 16545809 (1) (see all 6)
    n-acetylglucosamine 85.76 32 10824116 (6), 9468341 (4), 11308027 (3), 1312935 (2) (see all 14)
    carbamoyl phosphate 61.99 1 17425781 (1)
    sialic acid 54.52 1 1312935 (1)
    atp 43.65 8 12875848 (1), 17010375 (1), 10824116 (1), 19006331 (1) (see all 5)
    adp 42.57 3 17010375 (2), 19013524 (1)
    mannose 36.64 1 11298769 (1)
    glucose 15.41 5 17010375 (2), 17229727 (1), 11298769 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NAGK)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NAGK)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_017567

                  Sigma-Aldrich siRNA and siRNA Panels for NAGK  
                         Sigma-Aldrich shRNA Panels and shRNA for NAGK  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_017567  

    REFSEQ mRNAs for NAGK gene: 

    NM_017567.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_017567  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_017567
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_017567
                                     untagged cDNA clones in CMV expression vector (see all 3): AK092876 

    Additional cDNA sequence: 

    AJ242910.1 AK001812.1 AK092876.1 AK097223.1 AK222645.1 AK297224.1 BC001029.2 BC005371.1 
    BC131781.1 CR457271.1 CR591536.1 CR609075.1 CR615452.1 CR622455.1 CR622456.1 

    18 DOTS entries:

    DT.416482  DT.99977142  DT.97809944  DT.92436699  DT.100028615  DT.305375  DT.92436700  DT.92436698 
    DT.95300813  DT.120957174  DT.100641713  DT.120957173  DT.120957190  DT.92436696  DT.92436697  DT.95143517 
    DT.95294218  DT.97809003 

    24/279 AceView cDNA sequences (see all 279 ):

    BP338817 BE856167 AW298245 CR622455 BM691183 AI146728 CR615452 F12441 
    BI918367 AL832837 CA308248 CR622456 BG575875 BM982771 AI694016 CR609075 
    NM_017567 BM716173 W16890 CD370002 AA369452 BE410198 BQ436233 BF725735 

    highest scoring ESTs for NAGK:

    AW026312 AA039704 AA053240 AA058760 AA281320 AA443840 AA446627 AA452800 AA452936 AA502611 

    Unigene Cluster for NAGK:

    N-acetylglucosamine kinase
    Hs.7036  [show with all ESTs]
    Unigene Representative Sequence: AK097223


    GeneLoc Exon Structure

    5/18 Alternative Splicing Database (ASD) splice patterns (SP) for NAGK (see all 18 )

    ExUns: 1a · 1b · 1c · 1d · 1e · 1f · 1g · 1h · 1i ^ 2a · 2b · 2c · 2d ^ 3 ^ 4a · 4b ^ 5a · 5b · 5c · 5d ^ 6a · 6b ^ 7a · 7b · 7c · 7d ^
    SP1:                                            -     -     -                 -                       -                       -     -     -     -               
    SP2:                                                                          -                       -                       -     -     -     -               
    SP3:                                                                          -                       -                       -     -     -     -               
    SP4:                                            -     -     -                 -                       -                       -     -     -                     
    SP5:                                            -     -     -     -     -     -                       -                       -     -     -     -               

    ExUns: 8a · 8b · 8c · 8d · 8e · 8f · 8g ^ 9 ^ 10a · 10b ^ 11a · 11b · 11c
    SP1:  -     -                       -     -                                       
    SP2:  -     -                       -     -                                       
    SP3:  -     -     -                 -     -                                       
    SP4:                                                                              
    SP5:  -     -                                                                     

    About this scheme

    ECgene alternative splicing isoforms for NAGK

    1 Ensembl transcript including schematic representation:
    ENST00000244204  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    NAGK expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for NAGK

    1 / 2 / 3

    4 probe-sets matching NAGK gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    46246_at2, 3 U95-B 1 1.00 1.00 0.82 1.50 AW026312 1.00 1.00 1.00 1

    39527_at*2, 3 U95-A 9 1.00 0.11 0.62 0.51 AF090102 0.40 0.05 0.29 2

    218231_at2, 3 U133-A 1 1.00 1.00 -- -- NM_017567 0.60 1.00 0.82 1

    218231_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GACTATAGCG

    SOURCE GeneReport for Unigene cluster: Hs.7036

    UniProtKB/Swiss-Prot: NAGK_HUMAN, Q9UJ70
    Tissue specificity: Ubiquitous

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for NAGK gene from 5/11 species (see all 11 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    NAGK1   -- N-acetylglucosamine kinase 90.31(n)
    91.28(a)
    492060  XM_549180.2  XP_549180.2 
    chimpanzee
    (Pan troglodytes)
    NAGK1   -- N-acetylglucosamine kinase 99.22(n)
    99.13(a)
    459309  XM_515537.2  XP_515537.2 
    cow
    (Bos taurus)
    NAGK1   -- N-acetylglucosamine kinase 91.28(n)
    93.02(a)
    508132  NM_001034314.1  NP_001029486.1 
    rat
    (Rattus norvegicus)
    Nagk1   -- N-acetylglucosamine kinase 87.66(n)
    92.42(a)
    297393  NM_001037768.1  NP_001032857.1 
    mouse
    (Mus musculus)
    Nagk1, 5 65
    N-acetylglucosamine kinase1, 5 87.27(n)1
    91.84(a)1
    561741  NM_019542.11  NP_062415.11 
     AJ2429095  AJ2429115  (see all 9)
    About this table        Species with no ortholog for NAGK

    ENSEMBL Gene Tree for NAGK
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/64 NCBI SNPs in NAGK are shown (see all 64 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 56)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 2 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs22873291,2
    A,C,F,H,O71148562(-) CGGCTT/GTACAA 1 -- ng51 tfbs313Minor allele frequency- G:0.15NA MN EA EU WA 2228
    rs22873301,2
    C,F,H71148221(-) TTAAGC/GTTTCT 1 -- ng519Minor allele frequency- G:0.22EA EU WA 2194
    rs118902381,2
    C,F,H71147636(+) TTAAGA/GTCTGT 1 -- ng51 tfbs311Minor allele frequency- G:0.17EU EA WA NA 980
    rs22873311,2
    F71148060(-) GCCTAT/CGGCAT 1 -- ng511Minor allele frequency- C:0.43EA 1478
    --
    rs18333511,2
    C71147118(-) caacaG/Tggcga 1 -- ng510--------
    rs67131,2
    A,C,F,H71158964(-) CGCCAA/GAAGAA 1 L/L syn1 ese315Minor allele frequency- G:0.13NA EU EA WA 1398
    rs22873281,2
    A,C,F,H71151203(-) AGTCCA/GTCTGC 1 D/D syn1 ese310Minor allele frequency- G:0.16EA NA EU WA 2228
    rs10437011,2
    C,F,H71158246(+) TCTGTG/ATGGAA 1 V/V syn1 ese39Minor allele frequency- A:0.02MN EU EA WA 952
    rs115396221,2
    H71152434(+) GATGCC/AGCCGG 1 A/A syn1 ese34Minor allele frequency- A:0.00EU EA WA 420
    --
    rs24188931,2
    A71151456(+) GATCCA/TCTGGT 1 P/P syn1 ese30--------
    About this table

    HapMap Linkage Disequilibrium images for NAGK (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 606828

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/36 PubMed articles for NAGK gene (see all 36 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 55577 HGNC: 17174 AceView: NAGK Ensembl:ENSG00000124357 euGenes: HUgn55577
    ECgene: NAGK H-InvDB: NAGK
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for NAGK:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for NAGK-related products

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     Predesigned and custom siRNAs for NAGK Antibodies for NAGK
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for NAGK Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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