NAF1 Gene
protein-coding GIFtS: 47
GCID: GC04M164047
|
|
nuclear assembly factor 1 homolog (S. cerevisiae)
| |
Aliases
for NAF1 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Nuclear Assembly Factor 1 Homolog (S. Cerevisiae)1 2 | | H/ACA Ribonucleoprotein Complex Non-Core Subunit NAF12 | | HNAF11 | | KIAA01135 | | TNIP15 |
Export aliases for NAF1 gene to outside databasesPrevious GC identifers: GC04M164270 GC04M159801 |
Summaries
for NAF1 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: NAF1_HUMAN, Q96HR8Function: RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. Duringassembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complexand is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for bindingwith DKC1/NOLA4
|
Genomic Views
for NAF1 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000004.11 NC_018915.1 NT_016354.19
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the NAF1 gene promoter:
STAT1 p300 STAT1beta HSF1 (long) STAT1alpha MEF-2A AREB6 HFH-1 HSF1short aMEF-2
Other transcription factors
Search SABiosciences Chromatin IP Primers for NAF1
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NAF1 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 4q32.2 Ensembl cytogenetic band: 4q32.2 HGNC cytogenetic band: 4q32.2NAF1 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 4 GeneLoc Exon Structure GeneLoc location for GC04M164047: view genomic region
(about GC identifiers)
Start:
|
164,031,225 bp from pter |
End:
|
164,088,073 bp from pter |
Size:
|
56,849 bases |
Orientation:
|
minus strand |
|
Proteins
for NAF1 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: NAF1_HUMAN, Q96HR8 (See
protein sequence)Recommended Name: H/ACA ribonucleoprotein complex non-core subunit NAF1 Size: 494 amino acids; 53717 Da
Subunit: During assembly of the complex, component of the small nucleolar ribonucleoprotein particles containingH/ACA-type snoRNAs (H/ACA snoRNPs) which contains NOLA2/NHP2, NOLA3/NOP10, NAF1 and DKC1/NOLA4. Interacts directlywith DKC1/NOLA4
Subcellular location: Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus. Absent from thenucleolus (By similarity)
1 PDB 3D structure from  and Proteopedia  for NAF1:2EQN (3D)
Secondary accessions: D3DP28 E9PAZ2Alternative splicing: 2 isoforms: Q96HR8-1 Q96HR8-2 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for NAF1: NX_Q96HR8
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q96HR8
NAF1 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_001122403.1 NP_612395.2
ENSEMBL proteins: ENSP00000427518 ENSP00000408963 ENSP00000274054
Human Recombinant Protein Products:
Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view): About this table
NAF1 for ontologies About GeneDecksing
NAF1 Antibody Products:
Assay Products for NAF1: |
Protein
Domains /
Families
for NAF1 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
NAF1 for domains About GeneDecksing
2 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q96HR8ProtoNet protein and cluster: Q96HR8 UniProtKB/Swiss-Prot: NAF1_HUMAN, Q96HR8Similarity: Belongs to the NAF1 family |
Function
for NAF1 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: NAF1_HUMAN, Q96HR8Function: RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. Duringassembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complexand is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for bindingwith DKC1/NOLA4
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NAF1 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NAF1 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NAF1 |
Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view): About this table
NAF1 for ontologies About GeneDecksing
|
Pathways & Interactions
for NAF1 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for NAF1
STRING Interaction
Network Preview (showing 5 interactants - click image to see 17)
5/17 Interacting proteins for NAF1 (Q96HR82, 3 ENSP000002740544) via UniProtKB, MINT, STRING, and/or I2D (see all 17)About this table
Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table
NAF1 for ontologies About GeneDecksing
|
Drugs & Compounds
for NAF1 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for NAF1
Search CenterWatch for drugs/clinical trials and news about NAF1
|
Transcripts
for NAF1 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for NAF1 gene (2 alternative transcripts): NM_001128931.1 NM_138386.2 Unigene Cluster for NAF1: Nuclear assembly factor 1 homolog (S. cerevisiae) Hs.129095 [show with all ESTs]Unigene Representative Sequence: AK0973526 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000509434 ENST00000422287(uc010iqw.1) ENST00000274054(uc003iqj.3)
ENST00000509884 ENST00000509232 ENST00000502973
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NAF1 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NAF1 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 2): NAF1 (NM_001128931) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for NAF1 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NAF1 |
Additional cDNA sequence: AK097352.1 AK308601.1 BC008207.1 4 DOTS entries: DT.406805 DT.100020829 DT.101961616 DT.99974448 24/49 AceView cDNA sequences (see all 49): CR611986 AI923378 AK126037 AI886391 BX103045 CR622366 AK097352 CF128149 BX096010 AA321245 BX107765 AW469161 NM_138386 BX383358 BI869579 BM463276 BX508033 AI652820 BF110552 CD385920 AI452422 BC008207 BG673991 AL559696
GeneLoc Exon Structure
4 Alternative Splicing Database (ASD) splice patterns (SP) for NAF1 About this scheme
| ExUns: | 1a | · | 1b | · | 1c | · | 1d | ^ | 2 | ^ | 3a | · | 3b | ^ | 4 | ^ | 5 | ^ | 6 | ^ | 7 | ^ | 8 | ^ | 9 | ^ | 10 |
|---|
|
| SP1: | | | | | | | | | | | - | | - | | | | | | | | | | - | | | | | | |
| SP2: | | | | | | | | | | | - | | - | | | | | | | | | | | | | | | | |
| SP3: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
ECgene alternative splicing isoforms for NAF1
|
Expression
for NAF1 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| NAF1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
See NAF1 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for NAF1
SOURCE GeneReport for Unigene cluster: Hs.129095
SABiosciences Custom PCR Arrays for NAF1
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NAF1
Browse OriGene validated miRNA SYBR primer pairs
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Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NAF1 |
Orthologs
for NAF1 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for NAF1 gene from 8/22 species (see all 22) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
mouse
(Mus musculus) |
Mammalia |
Naf11 , 5 |
nuclear assembly factor 1 homolog (S. cerevisiae)1, 5 |
79.22(n)1
76.62(a)1 |
|
8 (33.18 cM)5
2343441 NM_001163564.11 NP_001157036.11
668602175 |
chicken
(Gallus gallus) |
Aves |
NAF11 |
nuclear assembly factor 1 homolog (S. cerevisiae) |
64.22(n)
54.52(a) |
|
769997 XM_001233312.1 XP_001233313.1 |
lizard
(Anolis carolinensis) |
Reptilia |
NAF16 |
-- |
67(a) |
1 ↔ 1 |
5(126231086-126237336) |
African clawed frog
(Xenopus laevis) |
Amphibia |
Xl.258322 |
Xenopus laevis transcribed sequence with weak similarity more |
75.69(n) |
|
BG413749.1 |
zebrafish
(Danio rerio) |
Actinopterygii |
AI657832.12 |
-- |
73.28(n) |
|
AI657832.1 |
fruit fly
(Drosophila melanogaster) |
Insecta |
CG103416 |
-- |
18(a) |
1 ↔ 1 |
2L(18698719-18700645) |
thale cress
(Arabidopsis thaliana) |
eudicotyledons |
ATNAF16 |
nuclear assembly factor 1 |
11(a) |
1 ↔ 1 |
1(880496-883391) |
rice
(Oryza sativa) |
Liliopsida |
-- |
NAF1 domain containing protein, expressed |
13(a) |
1 ↔ 1 |
5(24675996-24679972) |
ENSEMBL Gene Tree for NAF1 (if available)
TreeFam Gene Tree for NAF1 (if available) |
Paralogs
for NAF1 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| -- |
Genomic Variants
for NAF1 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 6 variations for NAF1
5 CNVs: 92513 80548 80547 98943 80549
1 Indel: 12575 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing NAF1 |
|
Disorders
/ Diseases
for NAF1 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
NAF1 for disorders About GeneDecksing
OMIM gene information: 607714
OMIM disorders: --
4  diseases for NAF1: About MalaCardspapillon-lefevre disease dyskeratosis congenita dyskeratosis hepatitis
Human Genome Epidemiology (HuGE) Navigator: NAF1 (15 documents)
Export disorders for NAF1 gene to outside databases
|
Publications
for NAF1 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for NAF1 gene, integrated from 9 sources (see all 25):
(articles sorted by number of sources associating them with NAF1) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- hNaf1 is required for accumulation of human box H/ACA snoRNPs, scaRNPs, and telomerase. (PubMed id 16601202)1, 2, 3 Hoareau-Aveilla C....Henry Y. (2006)
- Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells. (PubMed id 16618814)1, 2, 3 Darzacq X.... Meier U.T. (2006)
- Dynamic association and localization of human H/ACA RNP proteins. (PubMed id 17135485)1, 2 Kittur N....Meier U.T. (2006)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- Targeted ubiquitination and degradation of G-protein-c oupled receptor kinase 5 by the DDB1-CUL4 ubiquitin ligase complex. (PubMed id 22952844)1 Wu Z....Ma L. (2012)
- A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
- A high-throughput approach for measuring temporal chan ges in the interactome. (PubMed id 22863883)1 Kristensen A.R....Foster L.J. (2012)
- Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
- Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
- System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. (PubMed id 21406692)2 Rigbolt K.T....Blagoev B. (2011)
|
External Searches for NAF1 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing NAF1 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing NAF1 gene
(According to HUGE)
About This Section
| |
Specialized Databases showing NAF1 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for NAF1 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for NAF1 gene:
Search GeneIP for patents involving NAF1
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for NAF1 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for NAF1 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for NAF1 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NAF1 | | | OriGene Protein Over-expression Lysate for NAF1 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for NAF1 | | OriGene 3'-UTR Clone for NAF1 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NAF1 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NAF1 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
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| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NAF1 |
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