Home
GeneCards Guide
Suite
- MalaCards
- GeneALaCart
- GeneDecks
- GeneIP
- GeneLoc
Terms and Conditions
- Terms of Use
- 3rd Party Notice
About Us
User Feedback
Mirror sites

Set Analyses:

Generates a file of GeneCards annotations for your list of genes

Advanced Search

Search By

Section (entire)

for

All GeneCards Within gene subset (to enable gene prioritization):
or upload a file of gene symbols

Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

NAE1 Gene

protein-coding GIFtS: 61
GCID: GC16M066836

NEDD8 activating enzyme E1 subunit 1

(Previous names: amyloid beta precursor protein-binding protein 1, 59kD,...)
(Previous symbol: APPBP1)

Explore 5 diseases affiliated with
NAE1 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
NEDD8 Activating Enzyme E1 Subunit 1¹ ²		Proto-Oncogene Protein 1² ³
APPBP1¹ ² ³ ⁵		APP-BP1² ³
Amyloid Beta Precursor Protein Binding Protein 1, 59kDa¹ ²		Amyloid Beta Precursor Protein Binding Protein 1¹
Amyloid Beta Precursor Protein-Binding Protein 1, 59kD¹ ²		A-116A10.1²
Ula-1¹		NEDD8-Activating Enzyme E1 Regulatory Subunit²
Amyloid Beta Precursor Protein-Binding Protein 1, 59 KDa² ³		NEDD8-Activating Enzyme E1 Subunit²
Amyloid Protein-Binding Protein 1² ³		Protooncogene Protein 1²

External Ids:	HGNC: 621¹	Entrez Gene: 8883²	Ensembl: ENSG00000159593⁷	OMIM: 603385⁵	UniProtKB: Q13564³

Export aliases for NAE1 gene to outside databases

Previous GC identifers: GC16M065395 GC16M052710

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for NAE1:

The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell

surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of

Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a

ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three

transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: ULA1_HUMAN, Q13564

Function: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal

glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a

NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell

cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8

conjugation

Gene Wiki entry for NAE1 (APPBP1)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000016.9 NC_018927.1 NT_010498.15

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the NAE1 gene promoter:
SRF NF-1 Pax-5 Tal-1 p53 SRF (504 AA) E47 Ik-2
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: NAE1 promoter sequence

Search SABiosciences Chromatin IP Primers for NAE1

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NAE1

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16q22 Ensembl cytogenetic band: 16q22.1 HGNC cytogenetic band: 16q22

NAE1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NAE1 gene location

GeneLoc information about chromosome 16 GeneLoc Exon Structure

GeneLoc location for GC16M066836: view genomic region (about GC identifiers)

Start:	66,836,778 bp from pter	End:	66,907,159 bp from pter
Size:	70,382 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: ULA1_HUMAN, Q13564 (See protein sequence)

Recommended Name: NEDD8-activating enzyme E1 regulatory subunit

Size: 534 amino acids; 60246 Da

Subunit: Heterodimer of UBA3 and NAE1. The complex binds NEDD8 and UBE2M. Binds APP and TP53BP2

Subcellular location: Cell membrane. Note=Colocalizes with APP in lipid rafts

Miscellaneous: NAE1 and UBA3 correspond to the N-terminal and the C-terminal part of yeast UBA3. In yeast the two

subunits form a single polypeptide chain

6/9 PDB 3D structures from and Proteopedia for NAE1 (see all 9):

1R4M (3D)

1R4N (3D)

1TT5 (3D)

1YOV (3D)

2NVU (3D)

3DBH (3D)

Secondary accessions: A6NFN4 B2R700

Alternative splicing: 2 isoforms: Q13564-1 Q13564-2 (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for NAE1: NX_Q13564

Post-translational modifications:

Ubiquitinated by TRIP12, leading to its degradation by the proteasome¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q13564

NAE1 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (3 alternative transcripts):

NP_001018169.1 NP_001018170.1 NP_003896.1

ENSEMBL proteins:

ENSP00000463546 ENSP00000351990 ENSP00000290810 ENSP00000462937 ENSP00000368776

ENSP00000377637 ENSP00000455562 ENSP00000454471 ENSP00000454506 ENSP00000464119

ENSP00000456847 ENSP00000462040 ENSP00000455967 ENSP00000456564

Human Recombinant Protein Products for NAE1:

	Browse Purified and Recombinant Proteins at EMD Millipore
	R&D Systems Recombinant & Natural Proteins for NAE1 (NEDD8 Activating Enzyme (APPBP1/UBA3))
	Browse recombinant and purified proteins available from Enzo Life Sciences
	OriGene Purified Protein: NAE1 OriGene Protein Over-expression Lysate (see all 3): NAE1 OriGene Custom Protein Services for NAE1
	GenScript Custom Purified and Recombinant Proteins Services for NAE1
	Novus Biologicals NAE1 Proteins Novus Biologicals NAE1 Lysates
	Browse Sino Biological Recombinant Proteins
	ProSpec Recombinant Protein for NAE1
	Uscn Proteins for NAE1

Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005737	cytoplasm	TAS	8626687
GO:0005886	plasma membrane	IEA	--

NAE1 for ontologies About GeneDecksing

NAE1 Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse R&D Systems for Antibodies
	OriGene Antibodies: NAE1 OriGene Custom Antibody Services for NAE1
	GenScript Custom Superior Antibodies Services for NAE1
	Novus Biologicals NAE1 Antibodies
	Abcam antibodies for NAE1
	Uscn Antibodies for NAE1
	ThermoFisher Antibodies for NAE1

Assay Products for NAE1:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	R&D Systems Ubiquitin Proteasome Pathway (UPP) Assay Kits & Reagents for NAE1 (APPBP1, NEDD8 Activating Enzyme (APPBP1/UBA3))
	GenScript Custom Assay Services for NAE1
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for NAE1

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

NAE1 for domains About GeneDecksing

3 InterPro domains/families:

IPR009036 Molybdenum_cofac_synth_MoeB

IPR000594 ThiF_NAD_FAD-bd

IPR016040 NAD(P)-bd_dom

Graphical View of Domain Structure for InterPro Entry Q13564

ProtoNet protein and cluster: Q13564

1 Blocks protein family: IPB000594 UBA/THIF-type NAD/FAD binding fold

UniProtKB/Swiss-Prot: ULA1_HUMAN, Q13564

Similarity: Belongs to the ubiquitin-activating E1 family. ULA1 subfamily

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: ULA1_HUMAN, Q13564

Function: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal

glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a

NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell

cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8

conjugation

Enzyme regulation: Binding of TP53BP2 to the regulatory subunit NAE1 decreases neddylation activity

Gene Ontology (GO): 5/6 molecular function terms (GO ID links to tree view) (see all 6): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000166	nucleotide binding	IEA	--
GO:0003824	catalytic activity	--	--
GO:0005515	protein binding	IPI	15361859
GO:0019781	contributes to NEDD8 activating enzyme activity	IDA	12740388
GO:0031625	ubiquitin protein ligase binding	IPI	18627766

NAE1 for ontologies About GeneDecksing

Phenotypes:
3 GenomeRNAi human phenotypes for NAE1:

Decreased influenza A/WSN/33 r

Increased circadian period len

Synthetic lethal with Ras

Animal Models:

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for NAE1

miRNA Products:		OriGene 3'-UTR Clone (see all 3): NAE1 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat NAE1
		Search QIAGEN for miScript miRNA Assays for microRNAs that regulate NAE1
		Browse SwitchGear 3'UTR luciferase reporter plasmids

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for NAE1 (see all 4) OriGene shRNA RFP: NAE1 OriGene siRNA: NAE1
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NAE1
		Sirion Biotech Custom design and validation of potent shRNA sequences against NAE1

Gene Editing Products:			DNA2.0 Custom Protein Engineering Service for NAE1
			Sirion Biotech Customized adenovirus for overexpression of NAE1 Sirion Biotech Customized adenovirus for potent knockdown of NAE1

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NAE1 (see all 6) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NAE1 (see all 3) OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript Custom all cDNA clones Services for NAE1
		Browse Sino Biological Human cDNA Clones
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for NAE1
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NAE1

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for NAE1
		Search LifeMap BioReagents cell lines for NAE1
		Sirion Biotech Customized stable knockdown cell line services for NAE1 Sirion Biotech Customized inducible knockdown cell line services for NAE1 Sirion Biotech Customized stable overexpressing cell line services for NAE1 Sirion Biotech Customized inducible overexpressing cell line services for NAE1

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NAE1

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.

Alzheimer's disease

0.43

Alzheimers Disease

0.00

Pathway sources
See GeneCards unified pathways
Show all pathways

1 BioSystems Pathway for NAE1

Alzheimers Disease

1 Kegg Pathway (Kegg details for NAE1):

Alzheimer's disease

UniProtKB/Swiss-Prot: ULA1_HUMAN, Q13564

Pathway: Protein modification; protein neddylation

NAE1 for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for NAE1

STRING Interaction Network Preview (showing 5 interactants - click image to see 14)

5/89 Interacting proteins for NAE1 (Q13564^{2, 3} ENSP00000290810⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 89)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
UBE2M	P61081²^,³, ENSP00000253023⁴	MINT-6542674 I2D: score=3 STRING: ENSP00000253023
NEDD8	Q15843²^,³, ENSP00000250495⁴	MINT-6542674 I2D: score=5 STRING: ENSP00000250495
APP	P05067³, ENSP00000284981⁴	I2D: score=4 STRING: ENSP00000284981
UBA3	Q8TBC4³, ENSP00000354340⁴	I2D: score=13 STRING: ENSP00000354340
UIMC1	Q96RL1³, ENSP00000366434⁴	I2D: score=2 STRING: ENSP00000366434

About this table

Gene Ontology (GO): 5/7 biological process terms (GO ID links to tree view) (see all 7): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006260	DNA replication	IDA	14557245
GO:0006915	apoptotic process	IEA	--
GO:0007049	cell cycle	IEA	--
GO:0007165	signal transduction	TAS	8626687
GO:0033314	mitotic cell cycle DNA replication checkpoint	IDA	10722740

NAE1 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for NAE1

1 DrugBank Compound for NAE1 About this table

Compound	Synonyms	CAS #	Type	Actions	PubMed Ids
Adenosine triphosphate	5'-ATP (see all 8)	56-65-5	target	--	17139284 17016423

Search CenterWatch for drugs/clinical trials and news about NAE1 / ULA1

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for NAE1 gene (3 alternative transcripts):

NM_001018159.1 NM_001018160.1 NM_003905.3

Unigene Cluster for NAE1:

NEDD8 activating enzyme E1 subunit 1

Hs.460978 [show with all ESTs]

Unigene Representative Sequence: NM_001018159

18/22 Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 22):

ENST00000562428 ENST00000359087(uc002eqg.3 uc010cdv.3) ENST00000290810(uc002eqf.3)

ENST00000566663 ENST00000563221 ENST00000568672 ENST00000379463(uc002eqe.3)

ENST00000394074 ENST00000562074 ENST00000567521 ENST00000567743(uc010cdw.1)

ENST00000563253 ENST00000563185 ENST00000561579 ENST00000564040 ENST00000569388

ENST00000566336 ENST00000565535

miRNA Products:		OriGene 3'-UTR Clone (see all 3): NAE1 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat NAE1
		Search QIAGEN for miScript miRNA Assays for microRNAs that regulate NAE1
		Browse SwitchGear 3'UTR luciferase reporter plasmids

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for NAE1 (see all 4) OriGene shRNA RFP: NAE1 OriGene siRNA: NAE1
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NAE1
		Sirion Biotech Custom design and validation of potent shRNA sequences against NAE1

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NAE1 (see all 6) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NAE1 (see all 3) OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript Custom all cDNA clones Services for NAE1
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for NAE1
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NAE1

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NAE1 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat NAE1
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NAE1
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NAE1

Additional cDNA sequence:

AK097680.1 AK298159.1 AK307677.1 AK312784.1 AL136798.1 AY197612.1 BC000480.2 BC013301.2

BC041323.2 U50939.1

21 DOTS entries:

DT.86847561 DT.92448264 DT.97802551 DT.417295 DT.101982931 DT.95177720 DT.100796436 DT.40132947

DT.92448266 DT.100712589 DT.100796442 DT.95177762 DT.100646036 DT.100865362 DT.120675278 DT.91977513

DT.95177712 DT.100703919 DT.120675252 DT.40132949 DT.91698729

24/301 AceView cDNA sequences (see all 301):

BP371260 BF724456 H91836 BQ716155 BF724441 BU673985 BM978806 AA173544

BM793155 CA489574 CN481697 BC013301 AL042083 BU630112 AA353343 BE464580

AA373065 AU123884 BM827917 BQ576293 CA429735 BC000480 AW328270 CR614006

GeneLoc Exon Structure

5 Alternative Splicing Database (ASD) splice patterns (SP) for NAE1 About this scheme

ExUns:	1a	·	1b	^	2a	·	2b	^	3a	·	3b	^	4a	·	4b	^	5	^	6	^	7	^	8	^	9	^	10	^	11a	·	11b	^	12	^	13a	·	13b	^	14	^	15	^	16	^	17	^	18	^	19	^	20
SP1:											-				-														-								-
SP2:							-				-				-														-								-
SP3:							-				-
SP4:
SP5:							-

ECgene alternative splicing isoforms for NAE1

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

NAE1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TCACAAACTT

About this image

NAE1 expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

1 LifeMap In Vivo Development Anatomical Compartment/Cell

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Testis

Seminiferous Tubules

Secondary Spermatocyte

Germ Cells, Male Gametocytes

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

Stem Cell Differentiation: 1 LifeMap Cell

Name

Category

ES04(HES-4) (Embryonic Stem Cell)

Early Embryo, Inner Cell Mass

See NAE1 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for NAE1

SOURCE GeneReport for Unigene cluster: Hs.460978

UniProtKB/Swiss-Prot: ULA1_HUMAN, Q13564

Tissue specificity: Ubiquitous in fetal tissues. Expressed throughout the adult brain

SABiosciences Expression via Pathway-Focused PCR Arrays including NAE1:

Ubiquitination (Ubiquitylation) Pathway in human mouse rat

Alzheimer's Disease in human mouse rat

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NAE1 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat NAE1
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NAE1
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NAE1

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NAE1

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of eukaryotes.

Orthologs for NAE1 gene from 8/32 species (see all 32) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
chicken (Gallus gallus)	Aves	NAE1¹	NEDD8 activating enzyme E1 subunit 1	79.28(n) 84.08(a)		415792 NM_001006129.1 NP_001006129.1
lizard (Anolis carolinensis)	Reptilia	NAE1⁶	--	79(a)	1 ↔ 1	GL343295.1(198858-215729)
African clawed frog (Xenopus laevis)	Amphibia	Xl.32585²	Xenopus laevis transcribed sequence with moderate similarity more	79.19(n)		BU910737.1
zebrafish (Danio rerio)	Actinopterygii	zgc66148²	hypothetical protein MGC66148	73.43(n)		393471 BC055513.1
fruit fly (Drosophila melanogaster)	Insecta	APP-BP1¹	beta-Amyloid precursor protein binding protein 1	51.8(n) 46.17(a)		39244 NM_140178.4 NP_648435.1
worm (Caenorhabditis elegans)	Secernentea	ula-1⁶	NEDD8-activating enzyme E1 regulatory subunit	36(a)	1 ↔ 1	III(4918240-4920285)
thale cress (Arabidopsis thaliana)	eudicotyledons	AXL¹	amyloid beta precursor protein binding protein 1	50.52(n) 41.67(a)		817802 NM_128800.3 NP_180800.1
rice (Oryza sativa)	Liliopsida	Os.17427²	Oryza sativa (japonica cultivar-group) cDNA cloneJ more	75.04(n)		AK111534.1

ENSEMBL Gene Tree for NAE1 (if available)
TreeFam Gene Tree for NAE1 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for NAE1 gene

18/24 SIMAP similar genes for NAE1 using alignment to 14 protein entries: ULA1_HUMAN (see all proteins) (see all similar genes):

SUMO1 HBP1 FLJ00047 pp12301 KIAA0101 CDK10

RARS DYNC2LI1 MST132 TUBA1C EEF1E1 PARK2

PFKFB3 ADCK1 C21orf37 SEPSECS ARIH1 NLN

NAE1 for paralogs About GeneDecksing

1 Pseudogenes.org Pseudogene for NAE1
PGOHUM00000256677

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 16 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs78454486^1,2	F	--	66836404(+)	`AGAATG/TACCCC`	4	--	ds500¹ us2k¹		1		NA	120
rs181292622^1,2		--	66836484(+)	`ATGGTC/GTTGTT`	4	--	ds500¹ us2k¹		0	--	--	--	--
rs79934334^1,2	C	--	66836513(+)	`CCAGA-/AATAATAT`	4	--	ds500¹ us2k¹		0	--	--	--	--
rs363201^1,2	C,F,O	--	66836570(+)	`GTCCAT/CAGCTA`	4	--	ds500¹ us2k¹		5		NA WA	380
rs112257771^1,2	C	--	66836818(+)	`AATTTT/AGCAGC`	4	--	ut3¹ us2k¹		1		CSA	1
rs200209122^1,2		--	66836878(+)	`TTTCAA/GTCATT`	4	--	ut3¹ us2k¹		0	--	--	--	--
rs186346179^1,2	C	--	66836891(+)	`CACACA/TACTTA`	4	--	ut3¹ us2k¹		0	--	--	--	--
rs141151787^1,2	C,F	--	66836988(+)	`GTTTGG/CTGATT`	7	/T /S	mis¹ us2k¹		2		NA EU	5773
rs190706638^1,2	C	--	66837068(+)	`ACTTCA/GAGAGT`	4	--	int¹ us2k¹		0	--	--	--	--
rs363202^1,2	C	--	66837165(+)	`GAGTCA/GTATCA`	4	--	us2k¹ int¹		3		MN NA	188

HapMap Linkage Disequilibrium report for NAE1 (66836778 - 66907159 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for NAE1
1 CNV: 58769

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing NAE1

DNA2.0 Custom Variant and Variant Library Synthesis for NAE1

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

NAE1 for disorders           About GeneDecksing

OMIM gene information: 603385    OMIM disorders: --

5 diseases for NAE1:    About MalaCards

alzheimer's disease hepatitis e noma hepatitis

neuronitis

1 disease from the University of Copenhagen DISEASES database for NAE1:

Hepatitis E

Export disorders for NAE1 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for NAE1 gene, integrated from 9 sources (see all 44):
(articles sorted by number of sources associating them with NAE1)

Utopia: connect your pdf to the dynamic
world of online information

Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. (PubMed id 12740388)^{1, 2, 3} Bohnsack R.N. and Haas A.L. (2003)
APP-BP1, a novel protein that binds to the carboxyl-terminal region of the amyloid precursor protein. (PubMed id 8626687)^{1, 2, 3} Chow N.... Neve R.L. (1996)
APP-BP1 mediates APP-induced apoptosis and DNA synthesis and is increased in Alzheimer's disease brain. (PubMed id 14557245)^{1, 2, 9} Chen Y.... Neve R.L. (2003)
TRIP12 functions as an E3 ubiquitin ligase of APP-BP1. (PubMed id 18627766)^{1, 2} Park Y....Yoon J.B. (2008)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{1, 2} Ota T.... Sugano S. (2004)
A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8. (PubMed id 15361859)^{1, 2} Huang D.T.... Schulman B.A. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8. (PubMed id 12646924)^{1, 2} Walden H.... Schulman B.A. (2003)
The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1. (PubMed id 14690597)^{1, 2} Walden H.... Schulman B.A. (2003)
ASPP2 inhibits APP-BP1-mediated NEDD8 conjugation to cullin-1 and decreases APP-BP1-induced cell proliferation and neuronal apoptosis. (PubMed id 12694406)^{1, 2} Chen Y.... Neve R.L. (2003)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 8883	HGNC: 621	AceView: APPBP1	Ensembl:ENSG00000159593	euGenes: HUgn8883
ECgene: NAE1	Kegg: 8883	H-InvDB: NAE1

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for NAE1	Pharmacogenomics, SNPs, Pathways

Intellectual Property for NAE1 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

Patent Information for NAE1 gene:
Search GeneIP for patents involving NAE1

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, LifeMap BioReagents, and Sirion Biotech, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory)
About This Section


	EMD Millipore Custom Antibody & Bulk Services
	EMD Millipore Preclinical / Clinical Development Services
	EMD Millipore Immunoassay Services
	EMD Millipore Target Screening & Profiling Services

	Browse Antibodies		Browse Cell Culture Products
	Browse ELISAs		Browse Flow Cytometry Kits
	Browse Primer Pairs		Browse Kinase Activity Assays/Reagents
	Browse ELISpot Kits/Development Modules		Browse TFB/Immunoprecipitation Assays
	Browse Apoptosis Detection Kits/Reagents		Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents for NAE1 (APPBP1)
	Browse DNA Damage/Repair Kits/Reagents		Browse Multiplex/Array Assay Kits/Reagents
	Browse Cell Selection/Detection Kits/Reagents		Browse Secondary Antibodies/Controls/Staining Reagents
	Browse Phosphatase Activity Assays/Reagents		Recombinant/Natural Proteins for NAE1 (NEDD8 Activating Enzyme (APPBP1/UBA3))

	OriGene Antibodies for NAE1		OriGene shRNA RFP for NAE1
	OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for NAE1		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NAE1
	OriGene Protein Over-expression Lysate for NAE1		Browse OriGene Fluorogenic Cell Assay Kits
	OriGene siRNA for NAE1		OriGene 3'-UTR Clone for NAE1
	OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NAE1		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NAE1
	Browse OriGene GFP tagged cDNA clones in CMV expression vector		Browse OriGene MicroRNA Expression Plasmids
	Browse OriGene basic RS shRNAs		Browse OriGene validated miRNA SYBR primer pairs
	OriGene Purified Protein for NAE1		OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
	OriGene Custom Antibody Services for NAE1		OriGene Custom Protein Services for NAE1
	OriGene Custom Immunoassay Development

	QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat NAE1
	QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing NAE1
	QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NAE1
	QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NAE1
	QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NAE1
	QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NAE1

	GenScript Custom Purified and Recombinant Proteins Services for NAE1		GenScript Custom cDNA clones with any tag delivered in your preferred vector Services for NAE1
	GenScript Custom Assay Services for NAE1		GenScript Custom Superior Antibodies Services for NAE1
	GenScript Custom overexpressing Cell Line Services for NAE1		CloneReady with Over 120,000 Genes
	Gene Synthesis: Any Gene in Any Vector		Vector-based siRNA and miRNA, Ready for Transfection
	Gene Mutant Library, Variants up to 10^11		Plasmid Preparation
	Custom Peptide Services

Search for Antibodies & Assays

	Regulatory tfbs in NAE1 promoter
	Search Chromatin IP Primers for NAE1
	RT² qPCR Primer Assay in human, mouse, rat NAE1
	GNC Network for NAE1
	SABiosciences PCR Arrays including human, mouse, rat NAE1

Search Tocris compounds for NAE1

	Browse Sino Biological Proteins and Antibodies
	Browse Sino Biological cDNA Clones
	Antibodies/Proteins Production Services
	Rabbit Monoclonal Antibody Platform
	Bulk Purchasing

Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

	Novus Tissue Slides
	NAE1 antibodies
	NAE1 proteins
	NAE1 lysates

	Antibodies for NAE1
	See all of Abcam's Antibodies, Kits and Proteins for NAE1
	Custom Antibody / Protein Production Service
	Bulk Purchasing
	Advantages of Rabbit Monoclonal antibodies
	Abcam protocols and scientific support

Recombinant Protein for NAE1

NAE1 Proteins, Antibodies, CLIAs, and ELISAs

Search LifeMap BioReagents cell lines for NAE1

	Gene Synthesis
	Protein Engineering
	Variant Library Synthesis
	Codon Optimization
	Protein Production and Purification

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NAE1

	Browse SwitchGear 3'UTR luciferase reporter plasmids for NAE1
	SwitchGear Promoter luciferase reporter plasmids for NAE1

ThermoFisher Antibodies for NAE1

Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NAE1

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for NAE1

Sirion Biotech Customized:

	stable knockdown cell line services for NAE1
	inducible knockdown cell line services for NAE1
	design and validation of potent shRNA sequences against NAE1
	adenovirus for potent knockdown of NAE1
	adenovirus for overexpression of NAE1
	stable overexpressing cell line services for NAE1
	inducible overexpressing cell line services for NAE1

GeneCards Homepage - Last full update: 18 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013 , 14 May 2013

View Random Gene

Category			VWF (GIFTS: 73) von Willebrand factor
GIFtS Group

The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

The GeneCards Human Gene Database

Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

Hot genes Disease genes NAE1 gene at Home site.

hostname: 356977-web1.xennexinc.com index build: 106 solr: 1.4

Advanced Search

Export list of displayed genes

NAE1 Gene

NEDD8 activating enzyme E1 subunit 1

Aliases
for NAE1 gene

Summaries
for NAE1 gene

Genomic Views
for NAE1 gene

Proteins
for NAE1 gene

Protein Domains /
Families
for NAE1 gene

Function
for NAE1 gene

Pathways & Interactions
for NAE1 gene

Drugs & Compounds
for NAE1 gene

Transcripts
for NAE1 gene

Expression
for NAE1 gene

Orthologs
for NAE1 gene

Paralogs
for NAE1 gene

Genomic Variants
for NAE1 gene

Disorders / Diseases
for NAE1 gene

Publications
for NAE1 gene

External Searches for NAE1 gene

Genome Databases showing NAE1 gene

Other Databases showing NAE1 gene

Specialized Databases showing NAE1 gene

Intellectual Property
for NAE1 gene

Products
for NAE1 gene

View Random Gene

Advanced Search

Export list of displayed genes

NAE1 Gene

NEDD8 activating enzyme E1 subunit 1

Aliasesfor NAE1 gene

Summariesfor NAE1 gene

Genomic Viewsfor NAE1 gene

Proteinsfor NAE1 gene

Protein Domains /Familiesfor NAE1 gene

Functionfor NAE1 gene

Pathways & Interactionsfor NAE1 gene

Drugs & Compoundsfor NAE1 gene

Transcriptsfor NAE1 gene

Expression for NAE1 gene

Orthologsfor NAE1 gene

Paralogsfor NAE1 gene

Genomic Variantsfor NAE1 gene

Disorders / Diseasesfor NAE1 gene

Publicationsfor NAE1 gene

External Searches for NAE1 gene

Genome Databases showing NAE1 gene

Other Databases showing NAE1 gene

Specialized Databases showing NAE1 gene

Intellectual Propertyfor NAE1 gene

Productsfor NAE1 gene

View Random Gene

Aliases
for NAE1 gene

Summaries
for NAE1 gene

Genomic Views
for NAE1 gene

Proteins
for NAE1 gene

Protein Domains /
Families
for NAE1 gene

Function
for NAE1 gene

Pathways & Interactions
for NAE1 gene

Drugs & Compounds
for NAE1 gene

Transcripts
for NAE1 gene

Expression
for NAE1 gene

Orthologs
for NAE1 gene

Paralogs
for NAE1 gene

Genomic Variants
for NAE1 gene

Disorders / Diseases
for NAE1 gene

Publications
for NAE1 gene

Intellectual Property
for NAE1 gene

Products
for NAE1 gene