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NADSYN1 Gene

protein-coding   GIFtS: 56

GC11P070841
NAD synthetase 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 6.3.5.1 3
FLJ10631 1, 2
FLJ36703 2
FLJ40627 2
Descriptions
NAD synthetase 1 2
NAD(+) synthase [glutamine-hydrolyzing] 3
NAD(+) synthetase 1 3
glutamine-dependent NAD synthetase 2
External Ids
HGNC: 298321
Entrez Gene: 551912
UniProtKB: Q6IA693
Ensembl: ENSG000001728907
Search outside databases for aliases for NADSYN1 gene

Previous GC identifers: GC11P071386 GC11P070890

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for NADSYN1:
Nicotinamide adenine dinucleotide (NAD) is a coenzyme in metabolic redox reactions, a precursor for
several cell signaling molecules, and a substrate for protein posttranslational modifications. NAD
synthetase (EC 6.3.5.1) catalyzes the final step in the biosynthesis of NAD from nicotinic acid
adenine dinucleotide (NaAD).[supplied by OMIM]

Gene Wiki entry for NADSYN1
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NADSYN1 gene  

Entrez Gene cytogenetic band: 11q13.4   Ensembl cytogenetic band:  11q13.4   HGNC cytogenetic band: 11q13.4

NADSYN1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P070841:     (about GC identifiers)

Start:
70,841,865 bp from pter
End:
70,890,232 bp from pter
Size:
48,368 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000011.8  NT_033927.7  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: NADE1_HUMAN, Q6IA69 (See protein sequence)
Recommended Name: Glutamine-dependent NAD(+) synthetase  
Size: 706 amino acids; 79294 Da
Subunit: Homohexamer
Secondary accessions: Q86SN2 Q9HA25 Q9NVM8

REFSEQ proteins: NP_060631.2  

ENSEMBL proteins: 
ENSP00000326424 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (NAD Synthetase)
Human Recombinant Proteins from Abnova (NADSYN1)
              OriGene Purified Recombinant Human Protein: NADSYN1 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005829 cytosol EXP12547821
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Antibodies for NADSYN1: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for NADSYN1
Browse R&D Systems for Antibodies
Antibodies from Abcam (NAD Synthetase), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (NADSYN1)
Novus Biologicals Antibodies for NADSYN1

Assays for NADSYN1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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4 InterPro domains/families:
 IPR003010 Ntlse/CNhydtse
 IPR014729 Rossmann-like_a/b/a_fold
 IPR003694 NAD_synthase
 IPR014445 Gln-dep_NAD_synthase_GAT


   GeneDecks  NADSYN1 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q6IA69

ProtoNet protein and cluster: Q6IA69

2 Blocks protein families:
IPB003010 Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase
IPB003694 NAD+ synthase


UniProtKB/Swiss-Prot: NADE1_HUMAN, Q6IA69
Similarity: In the C-terminal section; belongs to the NAD synthetase family
Similarity: Contains 1 CN hydrolase domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NADSYN1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (NADSYN1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018161

              Applied Biosystems Silencer® siRNAs for NADSYN1

              Sigma-Aldrich siRNA for NADSYN1  
                     Sigma-Aldrich shRNA for NADSYN1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for NADSYN1
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_018161
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_018161
                                 untagged cDNA clone in CMV expression vector: NM_018161 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_018161

UniProtKB/Swiss-Prot: NADE1_HUMAN, Q6IA69
Catalytic activity: ATP + deamido-NAD(+) + L-glutamine + H(2)O = AMP + diphosphate + NAD(+) +
L-glutamate
Enzyme Number (IUBMB): EC 6.3.5.1 

5/6 Gene Ontology (GO) molecular function terms (links to tree view) (see all 6 ):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0003952 NAD+ synthase (glutamine-hydrolyzing) activity IEA--
GO:0005515 protein binding IPI15231747
GO:0005524 ATP binding IEA--
GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Invitrogen iPath™ Online BioAtlas - Pathway for NADSYN1 (Maps provided by GeneGo):
 ATP metabolism

   GeneDecks  NADSYN1 for the pathways selected above  
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1 Millipore Pathway for NADSYN1
 NAD metabolism

   GeneDecks  NADSYN1 for the pathways selected above  
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for  NADSYN1  (Your Favorite Gene powered by Ingenuity) 
 Glutamate Metabolism
 Nicotinate and Nicotinamide Metabolism

   GeneDecks  NADSYN1 for the pathways selected above  
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2 Kegg Pathways  (Kegg details for NADSYN1):
 hsa00251 Glutamate metabolism
 hsa00760 Nicotinate and nicotinamide metabolism

   GeneDecks  NADSYN1 for the pathways selected above  
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UniProtKB/Swiss-Prot: NADE1_HUMAN, Q6IA69
Pathway: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (glutamine route):
step 1/1


5/20 Interacting proteins for NADSYN1 (ENSP000003264243 Q6IA691) via UniProtKB, MINT, and/or STRING (see all 20 )

InteractantInteraction Details
GeneCardExternal ID(s)
UPF1Q929001EBI-748610, EBI-373471
BST1ENSP000003717833STRING (score=.899)
CD38ENSP000002262793STRING (score=.899)
ENPP1ENSP000003509643STRING (score=.899)
ENPP3ENSP000003502653STRING (score=.899)
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2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006807 nitrogen compound metabolic process IEA--
GO:0009435 NAD biosynthetic process IEA--
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for NADSYN1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NADSYN1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (NADSYN1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018161

              Sigma-Aldrich siRNA for NADSYN1  
                     Sigma-Aldrich shRNA for NADSYN1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_018161  

REFSEQ mRNAs for NADSYN1 gene: 

NM_018161.4   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_018161  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_018161
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_018161
                                 untagged cDNA clone in CMV expression vector: NM_018161 

Additional cDNA sequence: 

AB091316.1 AK001493.1 AK022436.1 AK055452.1 AK094022.1 AK097940.1 AK097946.1 AK225700.1 
AL512694.1 BC003638.2 BC003666.2 BC020977.1 CR457286.1 

22 DOTS entries:

DT.413773  DT.100787908  DT.100036310  DT.95307509  DT.86844688  DT.91741720  DT.95107745  DT.100787916 
DT.99930233  DT.120683340  DT.413775  DT.97802378  DT.97856969  DT.99982468  DT.100787914  DT.120683339 
DT.92012346  DT.92034641  DT.97822559  DT.99964869  DT.87011015  DT.95107741 

24/263 AceView cDNA sequences (see all 263 ):

CD370172 AW263058 AA465522 AI927502 AA857884 BE158957 BU684632 BQ719229 
AI669045 AK097946 BC015237 AL530760 BX283748 BF514939 AI658748 CA487729 
BM728592 BG272124 AI744722 AI186846 AI921634 AU122486 BG289242 BF591019 

highest scoring ESTs for NADSYN1:

AA178924 AA417583 AA417691 AA432078 AA446879 AA447224 AA480502 AA514351 AA551669 AA573433 

Unigene Cluster for NADSYN1:

NAD synthetase 1
Hs.556986  [show with all ESTs]
Unigene Representative Sequence: BC020977


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000319023  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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NADSYN1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for NADSYN1

1 / 2 / 3

8 probe-sets matching NADSYN1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

51760_s_at2, 3 U95-C 1 1.00 1.00 0.89 1.41 AI669045 0.80 0.88 0.84 1

51763_at*2, 3 U95-C 1 0.62 1.00 0.82 0.63 AA621061 0.60 0.83 0.73 1
86309_at*2, 3 U95-D 1 0.44 1.00 0.71 0.97 AA420492 0.20 0.33 0.27 2

218840_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_018161 0.60 0.83 0.73 1

232946_s_at2, 3 U133-B 1 1.00 1.00 -- -- AL512694 0.40 0.75 0.60 1

218840_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

232946_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

1565906_at*2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  NADSYN1 for binary patterns associated with the probe-sets selected above  
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: AACAGACACA

SOURCE GeneReport for Unigene cluster: Hs.556986
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for NADSYN1 gene from 5/20 species (see all 20 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
NADSYN11   -- NAD synthetase 1 86.81(n)
88.94(a)
483674  XM_540795.2  XP_540795.2 
chimpanzee
(Pan troglodytes)
NADSYN11   -- NAD synthetase 1 98.3(n)
98.3(a)
451395  XM_001174076.1  XP_001174076.1 
cow
(Bos taurus)
NADSYN11   -- NAD synthetase 1 86.87(n)
90.37(a)
513400  NM_001034443.1  NP_001029615.1 
rat
(Rattus norvegicus)
Nadsyn11   -- NAD synthetase 1 82.84(n)
85.49(a)
353255  NM_181480.1  NP_852145.1 
mouse
(Mus musculus)
Nadsyn11, 5 75
NAD synthetase 11, 5 83.5(n)1
86.02(a)1
789141  NM_030221.11  NP_084497.11 
 AJ3053435  AJ3116125  (see all 13)
About this table        Species with no ortholog for NADSYN1

ENSEMBL Gene Tree for NADSYN1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
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10/351 NCBI SNPs in NADSYN1 are shown (see all 351 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 176)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 11 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs7513891,2
A,C,F,H,O70890655(-) ATAAAC/GAGACA 1 -- ng3122Minor allele frequency- G:0.25NA EA EU WA 1880
rs22763601,2
A,C,F,H70847195(+) CCCTTG/CTGGAG 1 L/V mis121Minor allele frequency- C:0.42EA NA EU WA 3382
rs20020641,2
A,C,F,H70841068(-) CTGTTT/CGACTT 1 -- ng5112Minor allele frequency- C:0.34EU EA WA NA 1060
rs122820601,2
C,F,H70890035(+) TGGACG/AGCGTG 1 S/G mis1 ese39Minor allele frequency- A:0.01EU EA WA 1010
rs79504411,2
C,F,H70862326(+) CACCAC/AGTGCT 1 H/Q mis19Minor allele frequency- A:0.01EU EA WA 986
rs71211061,2
C,F70869465(+) GAGCCC/TCTACC 1 P/L mis1 ese32Minor allele frequency- T:0.06NA EA 248
--
rs116008601,2
A,C70841667(+) GCACCC/TGAACG 1 -- ng510--------
--
rs116066111,2
A,C70841694(+) CCGGGC/GCGGGG 1 -- ng510--------
rs350079711,2
C,F70886185(+) GATATG/AGGGAT 1 I/M mis15Minor allele frequency- A:0.02NA EU EA WA 498
rs20020631,2
A,C70841124(-) TCGTGA/GCCTCG 1 -- ng510--------
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HapMap Linkage Disequilibrium images for NADSYN1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 608285

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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9 PubMed articles for NADSYN1 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 55191 HGNC: 29832 AceView: NADSYN1 Ensembl:ENSG00000172890 euGenes: HUgn55191
ECgene: NADSYN1 H-InvDB: NADSYN1
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for NADSYN1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for NADSYN1-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for NADSYN1 Antibodies for NADSYN1
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for NADSYN1 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
 Antibodies   Primer Pairs  
 Cell Culture Products   ELISAs  
 Flow Cytometry Kits   Protease Activity Assays & Reagents  
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 Recombinant & Natural Proteins  

 Recombinant Proteins (NADSYN1)
 Antibodies (NADSYN1)
 Chimera RNAi (NADSYN1)
 Custom Service for Mouse Mab
 Custom Service for Rabbit Pab from Full-length Protein
  
 Search for Antibodies & Assays

 Recombinant Proteins
(NAD Synthetase)
 Antibodies (NAD Synthetase)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for NADSYN1




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for NADSYN1

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