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Aliases for NADK2 Gene

Aliases for NADK2 Gene

  • NAD Kinase 2, Mitochondrial 2 3
  • NAD Kinase Domain-Containing Protein 1, Mitochondrial 3 4
  • Mitochondrial NAD Kinase 2 4
  • C5orf33 3 4
  • NADKD1 3 4
  • MNADK 3 4
  • Chromosome 5 Open Reading Frame 33 2
  • NAD Kinase Domain Containing 1 2
  • EC 4
  • DECRD 3

External Ids for NADK2 Gene

Previous HGNC Symbols for NADK2 Gene

  • C5orf33
  • NADKD1

Summaries for NADK2 Gene

Entrez Gene Summary for NADK2 Gene

  • This gene encodes a mitochondrial kinase that catalyzes the phosphorylation of NAD to yield NADP. Mutations in this gene result in 2,4-dienoyl-CoA reductase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

GeneCards Summary for NADK2 Gene

NADK2 (NAD Kinase 2, Mitochondrial) is a Protein Coding gene. Diseases associated with NADK2 include 2,4-dienoyl-coa reductase deficiency. GO annotations related to this gene include protein homodimerization activity and NAD+ kinase activity.

UniProtKB/Swiss-Prot for NADK2 Gene

  • Mitochondrial NAD(+) kinase that phosphorylates NAD(+) to yield NADP(+). Can use both ATP or inorganic polyphosphate as the phosphoryl donor. Also has weak NADH kinase activity in vitro; however NADH kinase activity is much weaker than the NAD(+) kinase activity and may not be relevant in vivo.

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NADK2 Gene

Genomics for NADK2 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for NADK2 Gene

36,192,589 bp from pter
36,242,279 bp from pter
49,691 bases
Minus strand

Genomic View for NADK2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for NADK2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NADK2 Gene

No data available for Regulatory Elements for NADK2 Gene

Proteins for NADK2 Gene

  • Protein details for NADK2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    NAD kinase 2, mitochondrial
    Protein Accession:
    Secondary Accessions:
    • B5MC93
    • Q6UTX5
    • Q96NM0

    Protein attributes for NADK2 Gene

    442 amino acids
    Molecular mass:
    49433 Da
    Quaternary structure:
    • Homodimer.

    Alternative splice isoforms for NADK2 Gene


neXtProt entry for NADK2 Gene

Proteomics data for NADK2 Gene at MOPED

Post-translational modifications for NADK2 Gene

  • Ubiquitination at Lys104
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for NADK2 Gene

Domains for NADK2 Gene

Graphical View of Domain Structure for InterPro Entry



  • Q4G0N4
  • Belongs to the NAD kinase family.
genes like me logo Genes that share domains with NADK2: view

No data available for Gene Families and Suggested Antigen Peptide Sequences for NADK2 Gene

Function for NADK2 Gene

Molecular function for NADK2 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties: Kinetic parameters: KM=0.022 mM for NAD(+) {ECO:0000269 PubMed:23212377}; KM=1.7 mM for ATP {ECO:0000269 PubMed:23212377}; KM=1.2 mM for tetrapolyphosphate {ECO:0000269 PubMed:23212377}; Vmax=0.091 umol/min/mg enzyme {ECO:0000269 PubMed:23212377};
UniProtKB/Swiss-Prot CatalyticActivity: ATP + NAD(+) = ADP + NADP(+).
UniProtKB/Swiss-Prot EnzymeRegulation: Inhibited by NADH, NADPH and NADP(+).
UniProtKB/Swiss-Prot Function: Mitochondrial NAD(+) kinase that phosphorylates NAD(+) to yield NADP(+). Can use both ATP or inorganic polyphosphate as the phosphoryl donor. Also has weak NADH kinase activity in vitro; however NADH kinase activity is much weaker than the NAD(+) kinase activity and may not be relevant in vivo.

Enzyme Numbers (IUBMB) for NADK2 Gene

Gene Ontology (GO) - Molecular Function for NADK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003951 NAD+ kinase activity IDA 23212377
GO:0005524 ATP binding IEA --
GO:0042803 protein homodimerization activity IDA 23212377
genes like me logo Genes that share ontologies with NADK2: view

Animal Model Products

CRISPR Products

miRNA for NADK2 Gene

miRTarBase miRNAs that target NADK2

No data available for Phenotypes , Animal Models , Transcription Factor Targeting and HOMER Transcription for NADK2 Gene

Localization for NADK2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NADK2 Gene


Gene Ontology (GO) - Cellular Components for NADK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005739 mitochondrion IDA 23212377
genes like me logo Genes that share ontologies with NADK2: view

No data available for Subcellular locations from COMPARTMENTS for NADK2 Gene

Pathways for NADK2 Gene

SuperPathways for NADK2 Gene

No Data Available

Interacting Proteins for NADK2 Gene

Gene Ontology (GO) - Biological Process for NADK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006741 NADP biosynthetic process IEA --
GO:0008152 metabolic process --
GO:0016310 phosphorylation IEA --
GO:0019674 NAD metabolic process IDA 23212377
genes like me logo Genes that share ontologies with NADK2: view

No data available for Pathways by source for NADK2 Gene

Transcripts for NADK2 Gene

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for NADK2

Primer Products

  • QuantiTect SYBR Green Assays in human,mouse,rat
  • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
  • QuantiFast Probe-based Assays in human,mouse,rat

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for NADK2 Gene

No ASD Table

Relevant External Links for NADK2 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for NADK2 Gene

mRNA expression in normal human tissues for NADK2 Gene

mRNA differential expression in normal tissues according to GTEx for NADK2 Gene

This gene is overexpressed in Liver (7.6).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for NADK2 Gene

mRNA Expression by UniProt/SwissProt for NADK2 Gene

Tissue specificity: Widely expressed.
genes like me logo Genes that share expressions with NADK2: view

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery for NADK2 Gene

Orthologs for NADK2 Gene

This gene was present in the common ancestor of animals.

Orthologs for NADK2 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia NADK2 36
  • 100 (a)
  • 99.7 (n)
  • 99.55 (a)
(Bos Taurus)
Mammalia NADK2 36
  • 91 (a)
  • 91.78 (n)
  • 95.7 (a)
(Canis familiaris)
Mammalia NADK2 35
  • 93.51 (n)
  • 96.61 (a)
(Mus musculus)
Mammalia Nadk2 35
  • 89.22 (n)
  • 94.65 (a)
Nadk2 36
  • 90 (a)
Nadkd1 16
(Monodelphis domestica)
Mammalia NADK2 36
  • 80 (a)
(Ornithorhynchus anatinus)
Mammalia NADK2 36
  • 81 (a)
(Rattus norvegicus)
Mammalia Nadk2 35
  • 89.4 (n)
  • 96.18 (a)
(Gallus gallus)
Aves NADK2 36
  • 74 (a)
  • 79.09 (n)
  • 83.8 (a)
(Anolis carolinensis)
Reptilia NADK2 36
  • 73 (a)
African clawed frog
(Xenopus laevis)
Amphibia Xl.2638 35
tropical clawed frog
(Silurana tropicalis)
Amphibia nadk2 35
  • 72.07 (n)
  • 76.6 (a)
Str.11200 35
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.7246 35
(Danio rerio)
Actinopterygii Dr.13238 35
nadk2 35
  • 66.43 (n)
  • 73.38 (a)
nadk2 36
  • 68 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP006767 35
  • 46.75 (n)
  • 40.53 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG8080 35
  • 46.65 (n)
  • 38.34 (a)
CG8080 36
  • 33 (a)
(Caenorhabditis elegans)
Secernentea Y17G7B.10 35
  • 51.25 (n)
  • 47.06 (a)
Y17G7B.10 36
  • 36 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 33 (a)
CSA.2100 36
  • 42 (a)
Species with no ortholog for NADK2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NADK2 Gene

Gene Tree for NADK2 (if available)
Gene Tree for NADK2 (if available)

Paralogs for NADK2 Gene

Selected SIMAP similar genes for NADK2 Gene using alignment to 4 proteins: Pseudogenes for NADK2 Gene

genes like me logo Genes that share paralogs with NADK2: view

No data available for Paralogs for NADK2 Gene

Variants for NADK2 Gene

Sequence variations from dbSNP and Humsavar for NADK2 Gene

SNP ID Clin Chr 05 pos Sequence Context AA Info Type MAF
rs991032 -- 36,209,860(+) CATGA(G/T)GTATT intron-variant
rs991033 -- 36,209,973(+) AGTCA(C/T)TGGCT intron-variant
rs991034 -- 36,210,293(+) TCCAC(A/C)AAAAT intron-variant
rs1364013 -- 36,218,037(+) ATAGC(C/T)TCCTT intron-variant
rs1364014 -- 36,218,229(+) AAAGC(A/T)TTCTT intron-variant

Relevant External Links for NADK2 Gene

HapMap Linkage Disequilibrium report
Human Gene Mutation Database (HGMD)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for NADK2 Gene

Disorders for NADK2 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (1) Diseases for NADK2 Gene including...

Search for NADK2 Gene in MalaCards »


  • 2,4-dienoyl-CoA reductase deficiency (DECRD) [MIM:616034]: A rare, autosomal recessive, inborn error of polyunsaturated fatty acids and lysine metabolism, resulting in mitochondrial dysfunction. Affected individuals have a severe encephalopathy with neurologic and metabolic abnormalities beginning in early infancy. Laboratory studies show increased C10:2 carnitine levels and hyperlysinemia. {ECO:0000269 PubMed:24847004}. Note=The disease is caused by mutations affecting the gene represented in this entry.
genes like me logo Genes that share disorders with NADK2: view

Publications for NADK2 Gene

  1. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4
  2. Identification and characterization of a human mitochondrial NAD kinase. (PMID: 23212377) Ohashi K. … Murata K. (Nat. Commun. 2012) 3 4
  3. MNADK, a novel liver-enriched mitochondrion-localized NAD kinase. (PMID: 23616928) Zhang R. (Biol Open 2013) 2 3
  4. Mitochondrial NADP(H) deficiency due to a mutation in NADK2 causes dienoyl-CoA reductase deficiency with hyperlysinemia. (PMID: 24847004) Houten S.M. … Wanders R.J. (Hum. Mol. Genet. 2014) 3 4
  5. The DNA sequence and comparative analysis of human chromosome 5. (PMID: 15372022) Schmutz J. … Rubin E.M. (Nature 2004) 4

Products for NADK2 Gene

Sources for NADK2 Gene

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