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NAB2 Gene

protein-coding   GIFtS: 57

GC12P055769
NGFI-A binding protein 2 (EGR1 binding protein 2)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
MADER 1, 2, 3
MGC75085 2
Descriptions
EGR-1-binding protein 2 3
EGR1 binding protein 2 2
Melanoma-associated delayed early response protein 2, 3
NGFI-A binding protein 2 2
NGFI-A binding protein 2 (EGR1 binding protein 2) 2
Protein MADER 3
External Ids
HGNC: 76271
Entrez Gene: 46652
UniProtKB: Q157423
Ensembl: ENSG000001668867
Search outside databases for aliases for NAB2 gene

Previous GC identifers: GC12P057457 GC12P057707 GC12P057199

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for NAB2:
This gene encodes a member of the family of NGFI-A binding (NAB) proteins, which function in the
nucleus to repress transcription induced by some members of the EGR (early growth response) family
of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins
through a conserved N-terminal domain, and repress transcription through two partially redundant
C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by
interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced
transcript variants have been described, but their biological validity has not been determined.
[provided by RefSeq]

UniProtKB/Swiss-Prot: NAB2_HUMAN, Q15742
Function: Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.
Isoform 2 lacks repression ability (By similarity)

Gene Wiki entry for NAB2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NAB2 gene  

Entrez Gene cytogenetic band: 12q13.3-q14.1   Ensembl cytogenetic band:  12q13.3   HGNC cytogenetic band: 12q13.3

NAB2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P055769:     (about GC identifiers)

Start:
55,768,944 bp from pter
End:
55,775,526 bp from pter
Size:
6,583 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000012.10  NT_029419.11  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: NAB2_HUMAN, Q15742 (See protein sequence)
Recommended Name: NGFI-A-binding protein 2  
Size: 525 amino acids; 56594 Da
Subunit: Homomultimers may associate with EGR1 bound to DNA (By similarity)
Subcellular location: Nucleus (By similarity). Note=Isoform 2 is not localized to the nucleus (By
similarity)
Secondary accessions: O76006 Q14797
Alternative splicing: 3 isoforms:  Q15742-1   Q15742-2   Q15742-3   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_005958.1  

    ENSEMBL proteins: 
    ENSP00000377439 ENSP00000350309 ENSP00000341491 ENSP00000300131 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Nab2)
    Human Recombinant Proteins from Abnova (NAB2)
                  OriGene Purified Recombinant Human Protein: NAB2 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    About this table

    Antibodies for NAB2: 
    Browse Antibodies Central at Invitrogen
    Millipore Mono- and Polyclonal Antibodies for the study of NAB2
    Sigma-Aldrich Antibodies for NAB2
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Nab2), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (NAB2)
    Novus Biologicals Antibodies for NAB2

    Assays for NAB2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR006989 Nab_centr
     IPR006988 Nab_N


       GeneDecks  NAB2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q15742

    ProtoNet protein and cluster: Q15742

    UniProtKB/Swiss-Prot: NAB2_HUMAN, Q15742
    Domain: The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates
    multimerization
    Domain: The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression
    Similarity: Belongs to the NAB family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NAB2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NAB2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005967

                  Applied Biosystems Silencer® siRNAs for NAB2

                  Sigma-Aldrich siRNA for NAB2  
                         Sigma-Aldrich shRNA for NAB2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for NAB2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005967
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005967
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_005967 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_005967

    UniProtKB/Swiss-Prot: NAB2_HUMAN, Q15742
    Function: Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.
    Isoform 2 lacks repression ability (By similarity)
    Induction: By serum and PMA stimulation

    Genatlas biochemistry entry for NAB2:
    NGFI-A (nerve growth factor induced clone A) binding protein 2 (ERG2 binding protein) highly
    expressed in brain and thymus,with an alternatively splicing isoform missing exon 3,and in
    vascular smooth muscle cell in response to injury

    10 MGI mutant phenotypes (inferred from 1 allele(MGI details for Nab2):

    behavior/neurologicalgrowth/sizelethality-postnatallimbs/digits/tailnervous system
    normalreproductive systemrespiratory systemskeletonskin/coat/nails

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003714 transcription corepressor activity TAS8668170
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Millipore Pathway for NAB2
     Transcription factors in neurogenesis

       GeneDecks  NAB2 for the pathways selected above  
    About GeneDecksing

     Gene Network CentralTM Interacting Genes and Proteins Network for  NAB2 


    5 Interacting proteins for NAB2 (ENSP000003001313) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    EGR1ENSP000002399383STRING (score=.981)
    EGR2ENSP000002424803STRING (score=.954)
    IL2ENSP000002267303STRING (score=.887)
    --ENSP000003705563STRING (score=.95)
    --ENSP000003610993STRING (score=.895)
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    GO:0007399 nervous system development TAS8668170
    GO:0008283 cell proliferation TAS8668170
    GO:0016481 negative regulation of transcription IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for NAB2
    1 Novoseek chemical compound relationship for NAB2 gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    zinc 27.41 1 9722618 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NAB2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NAB2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005967

                  Sigma-Aldrich siRNA for NAB2  
                         Sigma-Aldrich shRNA for NAB2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_005967  

    REFSEQ mRNAs for NAB2 gene: 

    NM_005967.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_005967  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005967
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005967
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_005967 

    Additional cDNA sequence: 

    AK314229.1 BC065931.1 CR603613.1 CR622165.1 U48361.1 X70991.1 

    12 DOTS entries:

    DT.92021908  DT.97846322  DT.97767371  DT.448512  DT.75172958  DT.100797695  DT.121175392  DT.92455131 
    DT.95371077  DT.95371081  DT.95220306  DT.95337045 

    24/73 AceView cDNA sequences (see all 73 ):

    BQ424118 BC007756 U48361 BM835228 BQ422441 NM_005967 BM552661 BC065931 
    BQ059284 BM559519 CR622165 BI767607 BQ956141 BM928408 BM558623 AU133683 
    AW163064 BM545671 BG678380 BF525913 BM810231 BG476950 CD621461 BX414813 

    highest scoring ESTs for NAB2:

    X70991 AA195032 AA242793 AA252180 AA333002 AA378071 AA434391 AA631087 AA670443 AA725011 

    Unigene Cluster for NAB2:

    NGFI-A binding protein 2 (EGR1 binding protein 2)
    Hs.159223  [show with all ESTs]
    Unigene Representative Sequence: NM_005967


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for NAB2

    ExUns: 1 ^ 2 ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b
    SP1:                          -                                             
    SP2:                          -                             -               
    SP3:                                                                        
    SP4:                                                                        

    About this scheme

    ECgene alternative splicing isoforms for NAB2

    4 Ensembl transcripts including schematic representations:
    ENST00000393858  ENST00000357680  ENST00000342556  ENST00000300131  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    NAB2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for NAB2

    1 / 2 / 3

    5 probe-sets matching NAB2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    40405_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 X70991 1.00 1.00 1.00 1

    216017_s_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

    212803_at2, 3 U133-A 1 1.00 1.00 -- -- BF337329 0.80 1.00 0.91 1

    216017_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    212803_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GGCATAAGAC

    SOURCE GeneReport for Unigene cluster: Hs.159223

    Expression variation in blood from EXPOLDB for NAB2

    UniProtKB/Swiss-Prot: NAB2_HUMAN, Q15742
    Tissue specificity: Widely expressed at low levels. Highly expressed in melanoma cell lines

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for NAB2 gene from 5/6 species (see all 6 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    NAB21   -- NGFI-A binding protein 2 (EGR1 binding protein 2) 92.7(n)
    98.1(a)
    481122  XM_538243.2  XP_538243.2 
    chimpanzee
    (Pan troglodytes)
    NAB21   -- NGFI-A binding protein 2 (EGR1 binding protein 2) 99.81(n)
    100(a)
    452004  XM_509154.2  XP_509154.2 
    cow
    (Bos taurus)
    NAB21   -- NGFI-A binding protein 2 (EGR1 binding protein 2) 93.52(n)
    98.29(a)
    504755  NM_001045897.1  NP_001039362.1 
    rat
    (Rattus norvegicus)
    Nab21   -- Ngfi-A binding protein 2 89.97(n)
    96.57(a)
    314910  XM_235224.4  XP_235224.3 
    mouse
    (Mus musculus)
    Nab21, 5 10 (70.00 cM)5
    Ngfi-A binding protein 21, 5 88.83(n)1
    95.24(a)1
    179371  NM_008668.11  NP_032694.11 
     AI3855565  AI4519075  (see all 14)
    About this table        Species with no ortholog for NAB2

    ENSEMBL Gene Tree for NAB2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for NAB2 gene
    NAB12  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/49 NCBI SNPs in NAB2 are shown (see all 49 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 59)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 12 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs3240101,2
    A,C,F,H55768519(-) CTTCTC/TCTGTG 1 -- ng516Minor allele frequency- T:0.39EU EA WA NA 594
    rs37591361,2
    F,H55767761(+) AGCCCC/TGGTCC 1 -- ng513Minor allele frequency- T:0.03EA 1666
    rs10572901,2
    C,F55772545(+) AACACG/ACTCTT 1 T/T syn1 ese32Minor allele frequency- A:0.06MN NA 276
    rs30249791,2
    C,F,H,O55774560(-) TGGGCT/ACAGTT 1 -- int110Minor allele frequency- A:0.04NS EU EA WA 926
    rs3240191,2
    A,C,F,H55772914(-) TCGACT/CGGCAG 1 -- int17Minor allele frequency- C:0.48EA WA NA 642
    rs3240201,2
    A,F,H55772300(+) GGAGGA/CGGCCC 1 -- int1 trp35Minor allele frequency- C:0.40MN EU EA WA 562
    rs3240171,2
    A,C55774081(-) CCGCCG/TGCCCA 1 -- int10--------
    rs3240181,2
    A55773996(-) CCCATA/CCCCAC 1 -- int10--------
    rs22332671,2
    --55768531(+) CGCACA/TGCCCA 1 -- ng510--------
    --
    rs351312161,2
    --55772984(+) GAACT-/GGGGAG 1 L/R fra10--------
    About this table

    HapMap Linkage Disequilibrium images for NAB2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 602381

    3 Novoseek disease relationships for NAB2 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    prostate carcinoma 42.13 4 11567222 (3), 16956712 (1)
    prostate cancer 23.36 1 11567222 (1)
    tumors 0.00 6 16552752 (1), 11567222 (1), 17207284 (1)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/42 PubMed articles for NAB2 gene (see all 42 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 4665 HGNC: 7627 AceView: NAB2 Ensembl:ENSG00000166886 euGenes: HUgn4665
    ECgene: NAB2 H-InvDB: NAB2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for NAB2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Predesigned and custom siRNAs for NAB2 Antibodies for NAB2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for NAB2 Browse small molecules at Sigma-Aldrich
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     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for NAB2




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for NAB2

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