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NAB1 Gene

protein-coding   GIFtS: 59

GC02P191222
NGFI-A binding protein 1 (EGR1 binding protein 1)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Descriptions
EGR-1-binding protein 1 3
EGR1 binding protein 1 2
NGFI-A binding protein 1 2
NGFI-A binding protein 1 (EGR1 binding protein 1) 2
Transcriptional regulatory protein p54 3
External Ids
HGNC: 76261
Entrez Gene: 46642
UniProtKB: Q135063
Ensembl: ENSG000001383867
Search outside databases for aliases for NAB1 gene

Previous GC identifers: GC02P189718 GC02P190226 GC02P191477 GC02P191716 GC02P191339

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: NAB1_HUMAN, Q13506
Function: Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2
(By similarity)

Gene Wiki entry for NAB1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NAB1 gene  

Entrez Gene cytogenetic band: 2q32.3-q33   Ensembl cytogenetic band:  2q32.2   HGNC cytogenetic band: 2q32.3-q33

NAB1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P191222:     (about GC identifiers)

Start:
191,222,041 bp from pter
End:
191,265,737 bp from pter
Size:
43,697 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000002.10  NT_005403.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: NAB1_HUMAN, Q13506 (See protein sequence)
Recommended Name: NGFI-A-binding protein 1  
Size: 487 amino acids; 54401 Da
Subunit: Homomultimers may associate with EGR1 bound to DNA (By similarity)
Subcellular location: Nucleus (By similarity)
PDB structures from and Proteopedia :
2YUF (3D)    
Secondary accessions: O75383 O75384 Q6GTU1 Q9UEV1
Alternative splicing: 2 isoforms:  Q13506-1   Q13506-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_005957.2  

    ENSEMBL proteins: 
    ENSP00000336894 ENSP00000349749 ENSP00000387089 ENSP00000386682 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
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    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (NAB1)
    Human Recombinant Proteins from Abnova (NAB1)
                  OriGene Purified Recombinant Human Protein: NAB1 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus NAS8668170
    About this table

    Antibodies for NAB1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for NAB1
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (NAB1), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for NAB1

    Assays for NAB1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR006989 Nab_centr
     IPR006986 Nab1_C
     IPR006988 Nab_N


       GeneDecks  NAB1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q13506

    ProtoNet protein and cluster: Q13506

    1 Blocks protein family: IPB006986 Nab1

    UniProtKB/Swiss-Prot: NAB1_HUMAN, Q13506
    Domain: The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates
    multimerization
    Domain: The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression
    Similarity: Belongs to the NAB family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NAB1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NAB1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005966

                  Applied Biosystems Silencer® siRNAs for NAB1

                  Sigma-Aldrich siRNA for NAB1  
                         Sigma-Aldrich shRNA for NAB1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005966
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005966
                                     untagged cDNA clone in CMV expression vector: NM_005966 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_005966

    UniProtKB/Swiss-Prot: NAB1_HUMAN, Q13506
    Function: Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2
    (By similarity)

    Genatlas biochemistry entry for NAB1:
    NGFI-A (nerve growth factor induced clone A) binding protein 1 (EGR1 binding protein) also
    repressor of EGR2

    10 MGI mutant phenotypes (inferred from 1 allele(MGI details for Nab1):

    behavior/neurologicalgrowth/sizelethality-postnatallimbs/digits/tailnervous system
    normalreproductive systemrespiratory systemskeletonskin/coat/nails

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0016564 transcription repressor activity NAS8668170
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  NAB1 


    3 Interacting proteins for NAB1 (ENSP000003368943) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    EGR1ENSP000002399383STRING (score=.99)
    EGR2ENSP000002424803STRING (score=.977)
    --ENSP000003705563STRING (score=.734)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006355 regulation of transcription, DNA-dependent NAS8668170
    GO:0016481 negative regulation of transcription IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for NAB1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NAB1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NAB1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005966

                  Sigma-Aldrich siRNA for NAB1  
                         Sigma-Aldrich shRNA for NAB1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_005966  

    REFSEQ mRNAs for NAB1 gene: 

    NM_005966.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_005966  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005966
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005966
                                     untagged cDNA clone in CMV expression vector: NM_005966 

    Additional cDNA sequence: 

    AF045451.1 AF045452.1 AK292446.1 AK296549.1 AK313542.1 BC035724.1 U47007.1 

    12 DOTS entries:

    DT.415016  DT.95374036  DT.100777911  DT.70105379  DT.100674142  DT.75104531  DT.95192760  DT.95374031 
    DT.120967271  DT.120967303  DT.120967255  DT.95075512 

    24/181 AceView cDNA sequences (see all 181 ):

    AA464835 AW949339 BM757930 AA243485 AA968597 AL703348 F11652 NM_005966 
    BE041507 BM847145 AW340316 CB528843 BM837857 AA287080 BG681694 BM467050 
    AU125407 AI884323 T30554 AF045451 CA314681 AA687766 AA994001 BI793037 

    highest scoring ESTs for NAB1:

    AF045451 AA243423 AA243485 AA287080 AA287081 AA325121 AA401060 AA401067 AA426439 AA437171 

    Unigene Cluster for NAB1:

    NGFI-A binding protein 1 (EGR1 binding protein 1)
    Hs.570078  [show with all ESTs]
    Unigene Representative Sequence: NM_005966


    GeneLoc Exon Structure

    5/10 Alternative Splicing Database (ASD) splice patterns (SP) for NAB1 (see all 10 )

    ExUns: 1 ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6a · 6b · 6c · 6d · 6e · 6f ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13a · 13b · 13c
    SP1:                    -           -     -                                                                                                         
    SP2:                                                                                                                                                
    SP3:                                                                                                        -                                       
    SP4:                                                                                            -     -           -                                 
    SP5:        -     -                 -     -     -                                                                                                   

    About this scheme

    ECgene alternative splicing isoforms for NAB1

    4 Ensembl transcripts including schematic representations:
    ENST00000337386  ENST00000357215  ENST00000409581  ENST00000409641  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    NAB1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for NAB1

    1 / 2 / 3

    7 probe-sets matching NAB1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    38692_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AF045451 1.00 1.00 1.00 1

    208047_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_005966 0.60 1.00 0.82 1

    209272_at2, 3 U133-A 1 1.00 1.00 -- -- AF045451 1.00 1.00 1.00 1

    211139_s_at2, 3 U133-A 2 1.00 0.77 -- -- AF045452 0.60 1.00 0.82 1

    208047_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    209272_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    211139_s_at2 U133Plus2 2 1.00 0.77 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AAACGTTTCC

    SOURCE GeneReport for Unigene cluster: Hs.570078

    Expression variation in blood from EXPOLDB for NAB1

    UniProtKB/Swiss-Prot: NAB1_HUMAN, Q13506
    Tissue specificity: The short isoform is found in a myeloid Leukemia cell line

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for NAB1 gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    NAB11   -- NGFI-A binding protein 1 (EGR1 binding protein 1) 80.73(n)
    85.8(a)
    488447  XM_545569.2  XP_545569.2 
    chimpanzee
    (Pan troglodytes)
    NAB11   -- NGFI-A binding protein 1 (EGR1 binding protein 1) 99.59(n)
    100(a)
    459832  XM_001166842.1  XP_001166842.1 
    cow
    (Bos taurus)
    NAB11   -- NGFI-A binding protein 1 (EGR1 binding protein 1) 92.2(n)
    95.89(a)
    516781  XM_594940.3  XP_594940.3 
    rat
    (Rattus norvegicus)
    Nab11   -- Ngfi-A binding protein 1 87.6(n)
    92.98(a)
    64824  NM_022856.1  NP_074047.1 
    mouse
    (Mus musculus)
    Nab11, 5 1 (27.00 cM)5
    Ngfi-A binding protein 11, 5 87.7(n)1
    92.99(a)1
    179361  NM_008667.21  NP_032693.21 
     AB2216775  AI4510445  (see all 12)
    About this table        Species with no ortholog for NAB1

    ENSEMBL Gene Tree for NAB1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for NAB1 gene
    NAB22  

    Pseudogenes
    1 related pseudogene


    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/201 NCBI SNPs in NAB1 are shown (see all 201 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 90)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 2 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs22937651,2
    A,C,F,H191229090(+) GACTCC/AGAGTG 1 -- ut51 ese310Minor allele frequency- A:0.32EA EU WA NA 3145
    --
    rs64344101,2
    A,C,F191265990(+) AAGACA/GTTTTT 1 -- ng313Minor allele frequency- G:0.13NA 128
    rs126935821,2
    A,C191220184(+) TCAACC/TACTCC 1 -- ng510--------
    --
    rs75945181,2
    A,C191220239(+) ATTATA/GTGTAT 1 -- ng510--------
    rs102026421,2
    A,C191221010(+) TAATAC/GTTTGG 1 -- ng510--------
    rs133826041,2
    C191220275(+) TAACAC/TGATTT 1 -- ng510--------
    --
    rs10531761,2
    H191232895(+) AAATTC/TGGAAA 1 R/W mis14Minor allele frequency- T:0.00EU EA WA 404
    --
    rs10531751,2
    H191232878(+) TCCACA/GCAAAG 1 H/R mis1 ese35Minor allele frequency- G:0.00MN EU EA WA 590
    --
    rs109314671,2
    C191220684(+) ttcttC/Tttttt 1 -- ng51 trp30--------
    --
    rs22702321,2
    C,F,H191232639(-) GCATGG/ATGGCC 1 H/H syn1 ese38Minor allele frequency- A:0.04EA NA EU WA 1988
    About this table

    HapMap Linkage Disequilibrium images for NAB1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 600800

    1 PharmGKB disease relationship for NAB1 gene
    Disease PharmGKB Relations PubMed IDs for articles supporting these relationships
    Leukemia, Lymphocytic, Acute, L1FA  GN  12086872
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/20 PubMed articles for NAB1 gene (see all 20 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 4664 HGNC: 7626 AceView: NAB1 Ensembl:ENSG00000138386 euGenes: HUgn4664
    ECgene: NAB1 H-InvDB: NAB1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for NAB1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for NAB1 Antibodies for NAB1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for NAB1 Browse small molecules at Sigma-Aldrich
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     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for NAB1




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
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