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Aliases for NAALAD2 Gene

Aliases for NAALAD2 Gene

  • N-Acetylated Alpha-Linked Acidic Dipeptidase 2 2 3
  • Glutamate Carboxypeptidase III 2 3 4
  • N-Acetylated-Alpha-Linked Acidic Dipeptidase II 3 4
  • NAALADase II 3 4
  • EC 4 63
  • GCPIII 3 4
  • N-Acetylated Alpha-Linked Acidic Dipeptidase II 3
  • N-Acetylated-Alpha-Linked Acidic Dipeptidase 2 3
  • GPCIII 3

External Ids for NAALAD2 Gene

Previous GeneCards Identifiers for NAALAD2 Gene

  • GC11P092197
  • GC11P091374
  • GC11P090052
  • GC11P089555
  • GC11P089556
  • GC11P089508
  • GC11P089867
  • GC11P085952

Summaries for NAALAD2 Gene

Entrez Gene Summary for NAALAD2 Gene

  • This gene is a member of the N-acetylated alpha-linked acidic dipeptidase (NAALADase) gene family. The representative member of this family is the gene encoding human prostate-specific membrane antigen (PSM), which is a marker of prostatic carcinomas and is the first to be shown to possess NAALADase activity. NAALADase cleaves N-acetyl-L-aspartate-L-glutamate (NAAG), which is a neuropeptide expressed both in the central nervous systems and in the periphery and is thought to function as a neurotransmitter. The product of this gene is a type II integral membrane protein. Transient transfection of this gene confers both NAALADase and dipetidyl peptidase IV activities to mammalian cells. This gene is highly expressed in ovary and testis as well as within discrete brain areas. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

GeneCards Summary for NAALAD2 Gene

NAALAD2 (N-Acetylated Alpha-Linked Acidic Dipeptidase 2) is a Protein Coding gene. GO annotations related to this gene include metallopeptidase activity and carboxypeptidase activity. An important paralog of this gene is FOLH1.

UniProtKB/Swiss-Prot for NAALAD2 Gene

  • Has N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Also exhibits a dipeptidyl-peptidase IV type activity. Inactivate the peptide neurotransmitter N-acetylaspartylglutamate.

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NAALAD2 Gene

Genomics for NAALAD2 Gene

Regulatory Elements for NAALAD2 Gene

Genomic Location for NAALAD2 Gene

90,131,515 bp from pter
90,192,894 bp from pter
61,380 bases
Plus strand

Genomic View for NAALAD2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for NAALAD2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NAALAD2 Gene

Proteins for NAALAD2 Gene

  • Protein details for NAALAD2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    N-acetylated-alpha-linked acidic dipeptidase 2
    Protein Accession:
    Secondary Accessions:
    • B3KQR4
    • Q4KKV4
    • Q4VAM9

    Protein attributes for NAALAD2 Gene

    740 amino acids
    Molecular mass:
    83592 Da
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 2 Zn(2+) ions per subunit. Required for NAALADase activity.;
    Quaternary structure:
    • Homodimer.

    Three dimensional structures from OCA and Proteopedia for NAALAD2 Gene

    Alternative splice isoforms for NAALAD2 Gene


neXtProt entry for NAALAD2 Gene

Proteomics data for NAALAD2 Gene at MOPED

Selected DME Specific Peptides for NAALAD2 Gene

Post-translational modifications for NAALAD2 Gene

  • Glycosylation at Asn111, Asn143, Asn185, Asn314, Asn449, Asn603, and Asn628
  • Modification sites at PhosphoSitePlus

Other Protein References for NAALAD2 Gene

Domains for NAALAD2 Gene

Suggested Antigen Peptide Sequences for NAALAD2 Gene

Graphical View of Domain Structure for InterPro Entry



  • Q9Y3Q0
  • The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal
  • Belongs to the peptidase M28 family. M28B subfamily.
genes like me logo Genes that share domains with NAALAD2: view

Function for NAALAD2 Gene

Molecular function for NAALAD2 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates
UniProtKB/Swiss-Prot EnzymeRegulation:
Inhibited by quisqualate
UniProtKB/Swiss-Prot Function:
Has N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Also exhibits a dipeptidyl-peptidase IV type activity. Inactivate the peptide neurotransmitter N-acetylaspartylglutamate.

Enzyme Numbers (IUBMB) for NAALAD2 Gene

Gene Ontology (GO) - Molecular Function for NAALAD2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004180 carboxypeptidase activity NAS 10085079
GO:0008233 peptidase activity --
GO:0008236 serine-type peptidase activity NAS 10085079
GO:0008237 metallopeptidase activity IEA --
GO:0008239 dipeptidyl-peptidase activity NAS 10085079
genes like me logo Genes that share ontologies with NAALAD2: view

Animal Model Products

CRISPR Products

miRNA for NAALAD2 Gene

miRTarBase miRNAs that target NAALAD2

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for NAALAD2

In Situ Assay Products

Flow Cytometry Products

No data available for Phenotypes , Animal Models , Transcription Factor Targeting and HOMER Transcription for NAALAD2 Gene

Localization for NAALAD2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NAALAD2 Gene

Membrane; Single-pass type II membrane protein.

Subcellular locations from

Jensen Localization Image for NAALAD2 Gene COMPARTMENTS Subcellular localization image for NAALAD2 gene
Compartment Confidence
endoplasmic reticulum 2
cytoskeleton 1
cytosol 1
golgi apparatus 1
mitochondrion 1
plasma membrane 1

Gene Ontology (GO) - Cellular Components for NAALAD2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0016021 integral component of membrane NAS 10085079
genes like me logo Genes that share ontologies with NAALAD2: view

Pathways for NAALAD2 Gene

SuperPathways for NAALAD2 Gene

No Data Available

Interacting Proteins for NAALAD2 Gene

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)
Selected Interacting proteins: Q9Y3Q0-NALD2_HUMAN ENSP00000432481 for NAALAD2 Gene via I2D STRING

Gene Ontology (GO) - Biological Process for NAALAD2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006508 proteolysis NAS 10085079
GO:0042135 neurotransmitter catabolic process IEA --
genes like me logo Genes that share ontologies with NAALAD2: view

No data available for Pathways by source for NAALAD2 Gene

Transcripts for NAALAD2 Gene

Unigene Clusters for NAALAD2 Gene

N-acetylated alpha-linked acidic dipeptidase 2:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for NAALAD2

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for NAALAD2 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10a · 10b ^ 11 ^ 12a · 12b ^ 13a · 13b ^ 14 ^ 15 ^ 16a · 16b · 16c ^
SP1: - - - - - - - -
SP2: - -
SP3: -
SP4: - - - - - - - - - - - - - - - -
SP6: -

ExUns: 17 ^ 18 ^ 19 ^ 20 ^ 21
SP4: - - - -

Relevant External Links for NAALAD2 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for NAALAD2 Gene

mRNA expression in normal human tissues for NAALAD2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for NAALAD2 Gene

This gene is overexpressed in Pituitary (6.1).

Protein differential expression in normal tissues for NAALAD2 Gene

This gene is overexpressed in Fetal ovary (16.1), Ovary (14.3), Testis (11.1), Prostate (6.9), and Stomach (6.0).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for NAALAD2 Gene

SOURCE GeneReport for Unigene cluster for NAALAD2 Gene Hs.503560

mRNA Expression by UniProt/SwissProt for NAALAD2 Gene

Tissue specificity: Highest expression in the testis. Also found in ovary and spleen. Weak expression in prostate, heart and placenta. In brain, expressed in striatum, parietal cortex and ventral striatum with lower levels in hippocampus, brain stem, putamen and superior colliculus
genes like me logo Genes that share expressions with NAALAD2: view

Expression partners for NAALAD2 Gene

In Situ Assay Products

Orthologs for NAALAD2 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for NAALAD2 Gene

Organism Taxonomy Gene Similarity Type Details
(Bos Taurus)
Mammalia NAALAD2 35
  • 87.84 (n)
  • 86.35 (a)
  • 86 (a)
(Canis familiaris)
Mammalia NAALAD2 35
  • 89.82 (n)
  • 90.14 (a)
  • 83 (a)
(Mus musculus)
Mammalia Naalad2 35
  • 84.82 (n)
  • 88.11 (a)
Naalad2 16
Naalad2 36
  • 88 (a)
(Pan troglodytes)
Mammalia NAALAD2 35
  • 99.59 (n)
  • 99.73 (a)
  • 100 (a)
(Rattus norvegicus)
Mammalia Naalad2 35
  • 85.72 (n)
  • 88.38 (a)
(Monodelphis domestica)
Mammalia NAALAD2 36
  • 79 (a)
(Ornithorhynchus anatinus)
Mammalia NAALAD2 36
  • 78 (a)
(Gallus gallus)
Aves NAALAD2 35
  • 73.07 (n)
  • 74.42 (a)
  • 74 (a)
(Anolis carolinensis)
Reptilia NAALAD2 36
  • 72 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia naalad2 35
  • 70.11 (n)
  • 74.97 (a)
African clawed frog
(Xenopus laevis)
Amphibia Xl.15462 35
(Danio rerio)
Actinopterygii naalad2 36
  • 66 (a)
(Caenorhabditis elegans)
Secernentea C35C5.2 37
  • 32 (a)
R57.1 35
  • 49.93 (n)
  • 38.6 (a)
gcp-2.1 36
  • 33 (a)
gcp-2.2 36
  • 30 (a)
gcp-2.3 36
  • 28 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes VPS70 36
  • 21 (a)
VPS70 38
thale cress
(Arabidopsis thaliana)
eudicotyledons AT5G19740 35
  • 49.71 (n)
  • 39.68 (a)
(Hordeum vulgare)
Liliopsida Hv.3035 35
(Oryza sativa)
Liliopsida Os.18145 35
Os01g0740500 35
  • 46.62 (n)
  • 39.38 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU06285 35
  • 43.3 (n)
  • 32.9 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes SPBC354.09c 35
  • 42.61 (n)
  • 26.28 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 44 (a)
Species with no ortholog for NAALAD2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NAALAD2 Gene

Gene Tree for NAALAD2 (if available)
Gene Tree for NAALAD2 (if available)

Paralogs for NAALAD2 Gene

Paralogs for NAALAD2 Gene

Selected SIMAP similar genes for NAALAD2 Gene using alignment to 9 proteins:

genes like me logo Genes that share paralogs with NAALAD2: view

Variants for NAALAD2 Gene

Sequence variations from dbSNP and Humsavar for NAALAD2 Gene

SNP ID Clin Chr 11 pos Sequence Context AA Info Type MAF
rs472266 -- 90,190,085(-) GCAGC(A/C)AGCTA intron-variant
rs475613 -- 90,138,461(-) aaagc(A/C)aatga intron-variant
rs489009 -- 90,163,289(+) GGTTT(C/T)TTGAA intron-variant
rs492059 -- 90,133,820(-) TTCTT(C/T)CCTGA upstream-variant-2KB
rs493199 -- 90,140,441(+) gtgac(A/C)aacct intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NAALAD2 Gene

Variant ID Type Subtype PubMed ID
nsv898094 CNV Gain 21882294
dgv412e1 CNV Complex 17122850
nsv898118 CNV Gain 21882294
nsv898139 CNV Loss 21882294
nsv898140 CNV Loss 21882294
nsv898141 CNV Loss 21882294
esv1511526 CNV Insertion 17803354
nsv898142 CNV Gain 21882294
nsv468787 CNV Loss 19166990
esv272147 CNV Insertion 20981092

Relevant External Links for NAALAD2 Gene

HapMap Linkage Disequilibrium report

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NAALAD2 Gene

Disorders for NAALAD2 Gene

Relevant External Links for NAALAD2

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator

No disorders were found for NAALAD2 Gene.

No data available for MalaCards , OMIM , UniProtKB/Swiss-Prot , University of Copenhagen DISEASES , Novoseek inferred disease relationships and Genatlas for NAALAD2 Gene

Publications for NAALAD2 Gene

  1. Isolation and expression of novel human glutamate carboxypeptidases with N-acetylated alpha-linked acidic dipeptidase and dipeptidyl peptidase IV activity. (PMID: 10085079) Pangalos M.N. … Gordon R.D. (J. Biol. Chem. 1999) 2 3 4 23
  2. Identification of genes from a schizophrenia-linked translocation breakpoint region. (PMID: 11352574) Semple C.A. … Porteous D.J. (Genomics 2001) 3 23
  3. Signal sequence and keyword trap in silico for selection of full- length human cDNAs encoding secretion or membrane proteins from oligo- capped cDNA libraries. (PMID: 16303743) Otsuki T. … Isogai T. (DNA Res. 2005) 3 4
  4. Structural insight into the evolutionary and pharmacologic homology of glutamate carboxypeptidases II and III. (PMID: 19678840) Hlouchova K. … Lubkowski J. (FEBS J. 2009) 3 4
  5. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PMID: 20379614) Rose J.E. … Uhl G.R. (Mol. Med. 2010) 3 48

Products for NAALAD2 Gene

Sources for NAALAD2 Gene

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