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NAALAD2 Gene

protein-coding   GIFtS: 53

GC11P089508
N-acetylated alpha-linked acidic dipeptidase 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 3.4.17.21 3
MGC116996 2
MGC26353 2
NAADALASE2 1, 2
NAALADASE2 1, 2
Descriptions
N-acetylated alpha-linked acidic dipeptidase 2 2
N-acetylated alpha-linked acidic dipeptidase II 2
N-acetylated-alpha-linked acidic dipeptidase II 3
NAALADase II 3
External Ids
HGNC: 145261
Entrez Gene: 100032
UniProtKB: Q9Y3Q03
Ensembl: ENSG000000776167
Search outside databases for aliases for NAALAD2 gene

Previous GC identifers: GC11P092197 GC11P091374 GC11P090052 GC11P089555 GC11P089556

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for NAALAD2:
This gene is a member of the N-acetylated alpha-linked acidic dipeptidase (NAALADase) gene family.
The representative member of this family is the gene encoding human prostate-specific membrane
antigen (PSM), which is a marker of prostatic carcinomas and is the first to be shown to possess
NAALADase activity. NAALADase cleaves N-acetyl-L-aspartate-L-glutamate (NAAG), which is a
neuropeptide expressed both in the central nervous systems and in the periphery and is thought to
function as a neurotransmitter. The product of this gene is a type II integral membrane protein.
Transient transfection of this gene confers both NAALADase and dipetidyl peptidase IV activities
to mammalian cells. This gene is highly expressed in ovary and testis as well as within discrete
brain areas. [provided by RefSeq]

UniProtKB/Swiss-Prot: NALD2_HUMAN, Q9Y3Q0
Function: Has N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Also exhibits a
dipeptidyl-peptidase IV type activity. Inactivate the peptide neurotransmitter
N-acetylaspartylglutamate

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NAALAD2 gene  

Entrez Gene cytogenetic band: 11q14.3-q21   Ensembl cytogenetic band:  11q14.3   HGNC cytogenetic band: 11q14.3-q21

NAALAD2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P089508:     (about GC identifiers)

Start:
89,507,491 bp from pter
End:
89,565,424 bp from pter
Size:
57,934 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000011.8  NT_008984.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: NALD2_HUMAN, Q9Y3Q0 (See protein sequence)
Recommended Name: N-acetylated-alpha-linked acidic dipeptidase 2  
Size: 740 amino acids; 83592 Da
Cofactor: Binds 2 zinc ions per subunit. Required for NAALADase activity
Subcellular location: Membrane; Single-pass type II membrane protein (Potential)
Secondary accessions: Q4VAM9

REFSEQ proteins: NP_005458.1  

ENSEMBL proteins: 
ENSP00000320083 ENSP00000365111 


Human Recombinant Proteins 
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Browse recombinant and purified proteins available from Enzo Life Sciences
Browse Abcam for Recombinant Proteins
Human Recombinant Proteins from Abnova (NAALAD2)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016020 membrane IEA--
GO:0016021 integral to membrane NAS10085079
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Antibodies for NAALAD2: 
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Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for NAALAD2

Assays for NAALAD2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

3 InterPro domains/families:
 IPR007484 Peptidase_M28
 IPR007365 Transferrin_rcpt-like_dimerise
 IPR003137 PA


   GeneDecks  NAALAD2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q9Y3Q0

ProtoNet protein and cluster: Q9Y3Q0

3 Blocks protein families:
IPB003137 Protease-associated PA
IPB007365 Transferrin receptor-like dimerisation domain
IPB007484 Peptidase family M28


UniProtKB/Swiss-Prot: NALD2_HUMAN, Q9Y3Q0
Domain: The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type
activity in the C-terminal
Similarity: Belongs to the peptidase M28 family. M28B subfamily

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NAALAD2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005467

              Applied Biosystems Silencer® siRNAs for NAALAD2

              Sigma-Aldrich siRNA and siRNA Panels for NAALAD2  
                     Sigma-Aldrich shRNA for NAALAD2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for NAALAD2
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_005467
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_005467
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_005467 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_005467

UniProtKB/Swiss-Prot: NALD2_HUMAN, Q9Y3Q0
Function: Has N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Also exhibits a
dipeptidyl-peptidase IV type activity. Inactivate the peptide neurotransmitter
N-acetylaspartylglutamate
Catalytic activity: Release of an unsubstituted, C-terminal glutamyl residue, typically from
Ac-Asp-Glu or folylpoly-gamma-glutamates
Enzyme regulation: Inhibited by quisqualate
Enzyme Number (IUBMB): EC 3.4.17.21 

5/8 Gene Ontology (GO) molecular function terms (links to tree view) (see all 8 ):

GO IDQualified GO termEvidencePubMed IDs
GO:0004180 carboxypeptidase activity NAS10085079
GO:0008233 peptidase activity IEA--
GO:0008236 serine-type peptidase activity NAS10085079
GO:0008237 metallopeptidase activity IEA--
GO:0008239 dipeptidyl-peptidase activity NAS10085079
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Sigma-Aldrich "Your Favorite Gene" Pathway for  NAALAD2  (Your Favorite Gene powered by Ingenuity) 
 Lysine Degradation

   GeneDecks  NAALAD2 for the pathways selected above  
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1 Interacting protein for NAALAD2 (ENSP000003200833) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
DPP4ENSP000003537313STRING (score=.808)
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1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006508 proteolysis NAS10085079
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for NAALAD2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (NAALAD2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005467

              Sigma-Aldrich siRNA and siRNA Panels for NAALAD2  
                     Sigma-Aldrich shRNA for NAALAD2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_005467  

REFSEQ mRNAs for NAALAD2 gene: 

NM_005467.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_005467  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_005467
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_005467
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_005467 

Additional cDNA sequence: 

AJ012370.1 AK075390.1 AL133614.1 BC027594.1 BC038840.1 BC067793.1 BC096316.3 BC096317.3 
BC096318.3 BC099646.4 

5 DOTS entries:

DT.91678123  DT.100021398  DT.70104856  DT.120716399  DT.91678118 

24/52 AceView cDNA sequences (see all 52 ):

AI791615 BX110170 AK075390 AL133614 BC027594 CD242563 BC038840 BI524108 
BQ188040 AI698592 CD106541 BI597780 AI199807 AJ012370 BI600086 AI139622 
BX106487 BI598148 BU854265 NM_005467 BC067793 BV207937 BV194058 AW573201 

highest scoring ESTs for NAALAD2:

AJ012370 AK075390 BG185042 BQ438706 BU618706 BU854265 CD242563 AA424534 AA424600 AA973124 

Unigene Cluster for NAALAD2:

N-acetylated alpha-linked acidic dipeptidase 2
Hs.503560  [show with all ESTs]
Unigene Representative Sequence: NM_005467


GeneLoc Exon Structure

5/7 Alternative Splicing Database (ASD) splice patterns (SP) for NAALAD2 (see all 7 )

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10a · 10b ^ 11 ^ 12a · 12b ^ 13a · 13b ^ 14 ^ 15 ^ 16a · 16b · 16c ^
SP1:                          -                                               -           -                       -           -           -     -           -   
SP2:                          -                                                           -                                                                     
SP3:                          -                                                                                                                                 
SP4:                          -                                         -     -     -     -     -     -     -     -     -     -     -     -     -     -     -   
SP5:                                                                                                                                                            

ExUns: 17 ^ 18 ^ 19 ^ 20 ^ 21
SP1:                              
SP2:                              
SP3:                              
SP4:  -     -     -     -         
SP5:                              

About this scheme

ECgene alternative splicing isoforms for NAALAD2

2 Ensembl transcripts including schematic representations:
ENST00000321955  ENST00000375944  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

NAALAD2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for NAALAD2

1 / 2 / 3

7 probe-sets matching NAALAD2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

75117_at2, 3 U95-E 1 0.44 1.00 0.84 0.82 AI791615 0.60 1.00 0.82 1

76132_at2, 3 U95-D 3 0.38 0.36 0.66 1.20 AI791154 0.20 1.00 0.72 1

222161_at2, 3 U133-A 1 1.00 1.00 -- -- AJ012370 0.80 1.00 0.91 1

222161_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

1554506_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

1554507_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

1554504_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.503560

Expression variation in blood from EXPOLDB for NAALAD2

UniProtKB/Swiss-Prot: NALD2_HUMAN, Q9Y3Q0
Tissue specificity: Highest expression in the testis. Also found in ovary and spleen. Weak
expression in prostate, heart and placenta. In brain, expressed in striatum, parietal cortex and
ventral striatum with lower levels in hippocampus, brain stem, putamen and superior colliculus

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for NAALAD2 gene from 5/17 species (see all 17 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
NAALAD21   -- N-acetylated alpha-linked acidic dipeptidase 2 89.86(n)
90.14(a)
485139  XM_542257.2  XP_542257.2 
chimpanzee
(Pan troglodytes)
NAALAD21   -- N-acetylated alpha-linked acidic dipeptidase 2 99.59(n)
99.73(a)
451478  XM_001137810.1  XP_001137810.1 
cow
(Bos taurus)
NAALAD21   -- N-acetylated alpha-linked acidic dipeptidase 2 88.36(n)
86.96(a)
536439  XM_616570.3  XP_616570.3 
rat
(Rattus norvegicus)
Naalad21   -- N-acetylated alpha-linked acidic dipeptidase 2 85.72(n)
88.38(a)
300384  XM_235877.4  XP_235877.3 
mouse
(Mus musculus)
Naalad21, 5 9 (3.00 cM)5
N-acetylated alpha-linked acidic dipeptidase 21, 5 84.82(n)1
88.11(a)1
725601  NM_028279.31  NP_082555.21 
 AK0122705  AK1336115  (see all 14)
About this table        Species with no ortholog for NAALAD2

ENSEMBL Gene Tree for NAALAD2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for NAALAD2 gene
NAALADL12  FOLH12  ENSG000001346122  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/444 NCBI SNPs in NAALAD2 are shown (see all 444 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 156)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 11 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs18928871,2
A,C,F,H,O89507520(-) GGGCTG/ACAGAG 1 -- ut51 ese38Minor allele frequency- A:0.43EA NA EU WA 2042
rs10614701,2
A,C,F,H,O89565410(+) ATAAAC/TGTGAT 1 -- ut31 ese37Minor allele frequency- T:0.48EU EA WA NA 560
rs108304301,2
A,C,F,H89541802(+) CATCTA/GTAGAA 1 I/V mis1 ese38Minor allele frequency- G:0.14EU EA WA 838
--
rs11385311,2
A,C,F,H89565060(+) gaatgG/Tataaa 1 -- ut31 ese34Minor allele frequency- T:0.31EU EA MN 484
rs107652491,2
A,C,F,H89507062(+) GACAGT/GGATTG 1 -- ng514Minor allele frequency- G:0.11EU EA WA 372
rs38089781,2
A,C,F,H89505835(-) CTAATA/GACTTA 1 -- ng517Minor allele frequency- G:0.50NA EU EA WA 544
rs110188791,2
C,F,H89520252(+) AGTTGG/ATTCAT 1 I/V mis1 ese38Minor allele frequency- A:0.02EU EA WA 838
rs110189051,2
C,F,H89564827(+) AGGAAC/GATGAA 1 -- ut3112Minor allele frequency- G:0.21EU EA WA 1258
rs107413151,2
A,C,F89505665(+) AAATAA/GAAAAC 1 -- ng513Minor allele frequency- G:0.46NA 124
--
rs79465611,2
A,C89506797(+) gacccC/Tctggc 1 -- ng510--------
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HapMap Linkage Disequilibrium images for NAALAD2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 611636

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

9 PubMed articles for NAALAD2 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 10003 HGNC: 14526 AceView: NAALAD2 Ensembl:ENSG00000077616 euGenes: HUgn10003
ECgene: NAALAD2 H-InvDB: NAALAD2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for NAALAD2 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for NAALAD2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Lentivirus-delivered shRNAs for NAALAD2 Browse small molecules at Sigma-Aldrich
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