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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

NAAA Gene

protein-coding   GIFtS: 47
GCID: GC04M076831

N-acylethanolamine acid amidase

(Previous name: N-acylsphingosine amidohydrolase (acid ceramidase)-like...)
(Previous symbol: ASAHL)
 Explore 9 diseases affiliated with
NAAA via our new
 Human Malady Compendium 
Biological research products
for NAAA
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
N-Acylethanolamine Acid Amidase1 2     N-Acylsphingosine Amidohydrolase (Acid Ceramidase)-Like1
ASAHL1 2 3     N-Acylethanolamine-Hydrolyzing Acid Amidase2
Acid Ceramidase-Like Protein2 3     EC 3.5.1.-3
PLT2 3     N-Acylsphingosine Amidohydrolase-Like3
ASAH-Like Protein2 3     

External Ids:    HGNC: 7361   Entrez Gene: 271632   Ensembl: ENSG000001387447   OMIM: 6074695   UniProtKB: Q020833   

Export aliases for NAAA gene to outside databases

Previous GC identifers: GC04M077050 GC04M072584


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for NAAA:
This gene encodes an N-acylethanolamine-hydrolyzing enzyme which is highly similar to acid ceramidase. Multiple
transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: NAAA_HUMAN, Q02083
Function: Degrades bioactive fatty acid amides to their corresponding acids, with the following preference:
N-palmitoylethanolamine > N-myristoylethanolamine > N-lauroylethanolamine = N-stearoylethanolamine >
N-arachidonoylethanolamine > N-oleoylethanolamine. Also exhibits weak hydrolytic activity against the ceramides
N-lauroylsphingosine and N-palmitoylsphingosine

Gene Wiki entry for NAAA (ASAHL)


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000004.11  NC_018915.1  NT_016354.19  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the NAAA gene promoter:
         TBP   PPAR-gamma1   POU2F1   Sp1   POU2F1a   FOXJ2 (long isoform)   FOXJ2   PPAR-gamma2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidNAAA promoter sequence
   Search SABiosciences Chromatin IP Primers for NAAA

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NAAA


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4q21.1   Ensembl cytogenetic band:  4q21.1   HGNC cytogenetic band: 4q21.1

NAAA Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NAAA gene location

GeneLoc information about chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04M076831:  view genomic region     (about GC identifiers)

Start:
76,831,808 bp from pter      End:
76,862,204 bp from pter
Size:
30,397 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: NAAA_HUMAN, Q02083 (See protein sequence)
Recommended Name: N-acylethanolamine-hydrolyzing acid amidase precursor  
Size: 359 amino acids; 40066 Da
Subunit: Heterodimer of an alpha and a beta subunit, non-covalently linked
Subcellular location: Lysosome (Probable)
Secondary accessions: Q5KTF2 Q96EY2 Q9BRA8
Alternative splicing: 3 isoforms:  Q02083-1   Q02083-2   Q02083-3   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for NAAA: NX_Q02083

Post-translational modifications:

  • N-glycosylated. Tunicamycin treatment causes a reduction in specific activity against N-palmitoylethanolamine1
  • Autoproteolytic cleavage in the acidic lumen of the lysosome activates the enzyme1
  • View neXtProt modification sites for NX_Q02083

  • NAAA Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_001035861.1  NP_055250.2  

    ENSEMBL proteins: 
     ENSP00000286733   ENSP00000423397   ENSP00000427641   ENSP00000426977   ENSP00000423142  
     ENSP00000382420  

    Human Recombinant Protein Products: 
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    Uscn Proteins for NAAA

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IDA10610717
    GO:0005764lysosome IEA--


    NAAA for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    NAAA for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR003199 Chologlycine_hydro
     IPR016699 Acid_ceramidase-like

    Graphical View of Domain Structure for InterPro Entry Q02083

    ProtoNet protein and cluster: Q02083

    1 Blocks protein family: IPB003199 Choloylglycine hydrolase

    UniProtKB/Swiss-Prot: NAAA_HUMAN, Q02083
    Similarity: Belongs to the acid ceramidase family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: NAAA_HUMAN, Q02083
    Function: Degrades bioactive fatty acid amides to their corresponding acids, with the following preference:
    N-palmitoylethanolamine > N-myristoylethanolamine > N-lauroylethanolamine = N-stearoylethanolamine >
    N-arachidonoylethanolamine > N-oleoylethanolamine. Also exhibits weak hydrolytic activity against the ceramides
    N-lauroylsphingosine and N-palmitoylsphingosine
    Enzyme regulation: Stimulated by DTT and Nonidet P-40
    Biophysicochemical properties: Kinetic parameters: KM=97 uM for N-palmitoylethanolamine; pH dependence: Optimum pH is
    4.5 with N-palmitoylethanolamine as substrate;

    Enzyme Number (IUBMB): EC 3.5.1.-1

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    Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003674molecular_function ND--
    GO:0008134transcription factor binding IPI--
    GO:0016787hydrolase activity ----
    GO:0016810hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds IDA17980170


    NAAA for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for NAAA

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006629lipid metabolic process IEA--
    GO:0008150biological_process ND--


    NAAA for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    NAAA for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for NAAA
    2 Novoseek chemical compound relationships for NAAA gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    anandamide 79.1 4 17980170 (1), 18793752 (1)
    monoacylglycerol 76.7 5 16375680 (1), 19014633 (1)

    Search CenterWatch for drugs/clinical trials and news about NAAA 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for NAAA gene (2 alternative transcripts): 
    NM_001042402.1  NM_014435.3  

    Unigene Cluster for NAAA:

    N-acylethanolamine acid amidase
    Hs.437365  [show with all ESTs]
    Unigene Representative Sequence: NM_014435
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000286733(uc003hjb.3) ENST00000511606 ENST00000513045 ENST00000507956(uc003hjc.4 uc003hjd.4)
    ENST00000505594(uc011cbq.2) ENST00000507187(uc010iiz.1) ENST00000507940
    ENST00000503636 ENST00000399497(uc003hja.3)

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    hsa-miR-136 hsa-miR-1301
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    Inhib. RNA
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    Additional cDNA sequence: 

    AB161353.1 AK024677.1 AK301132.1 AK309271.1 M92449.1 

    15 DOTS entries:

    DT.86857920  DT.109510  DT.91959761  DT.211615  DT.91816216  DT.40111879  DT.121268169  DT.92426440 
    DT.95229006  DT.121268102  DT.95182109  DT.95288709  DT.95288708  DT.97840369  DT.99944948 

    24/140 AceView cDNA sequences (see all 140):

    BM847445 CA942081 AW614594 AI685903 BM725506 BM682921 AI241573 AI701976 
    AI088304 AI056658 AA426330 BQ051465 BQ189557 AI348673 AI580701 W47576 
    AA995567 AA974051 R09660 BM726877 BM682907 BM719630 AI697736 BM763257 

    GeneLoc Exon Structure

    5/7 Alternative Splicing Database (ASD) splice patterns (SP) for NAAA (see all 7)    About this scheme

    ExUns: 1 ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b · 12c
    SP1:        -           -                                                                                       
    SP2:        -           -                                                     -                                 
    SP3:        -           -                 -                                   -                                 
    SP4:                                                                          -     -                           
    SP5:                                                                                -                           


    ECgene alternative splicing isoforms for NAAA

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    NAAA expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TAATGGGAGT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See NAAA Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for NAAA

    SOURCE GeneReport for Unigene cluster: Hs.437365

    UniProtKB/Swiss-Prot: NAAA_HUMAN, Q02083
    Tissue specificity: Expressed in numerous tissues, with highest levels in liver and kidney, followed by pancreas

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for NAAA gene from 5/13 species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Naaa1 , 5 N-acylethanolamine acid amidase1, 5 80.9(n)1
    76.69(a)1
      5 (46.35 cM)5
    671111  NM_025972.41  NP_080248.21 
     922576595 
    chicken
    (Gallus gallus)
    Aves NAAA1 N-acylethanolamine acid amidase 68.48(n)
    65.62(a)
      422641  XM_420596.3  XP_420596.2 
    lizard
    (Anolis carolinensis)
    Reptilia NAAA6
    --
    66(a)
    1 ↔ 1
    5(146982851-146993285)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.98472 Xenopus laevis transcribed sequence with weak similarity more 80.16(n)    CD329942.1 
    worm
    (Caenorhabditis elegans)
    Secernentea Y55D5A.31 Protein Y55D5A.3 46.3(n)
    34.95(a)
      190304  NM_065246.1  NP_497647.1 


    ENSEMBL Gene Tree for NAAA (if available)
    TreeFam Gene Tree for NAAA (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for NAAA gene
    ASAH12  
    1 SIMAP similar gene for NAAA using alignment to 4 protein entries:     NAAA_HUMAN (see all proteins):
    ASAH1

    NAAA for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section
    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    NAAA for disorders           About GeneDecksing

    OMIM gene information: 607469    OMIM disorders: --

    9 diseases for NAAA:    About MalaCards
    farber lipogranulomatosis    autoimmune thrombocytopenic purpura    lipogranulomatosis    anorexia nervosa
    pancytopenia    purpura    alzheimer's disease    prostate cancer
    prostatitis

    2 diseases from the University of Copenhagen DISEASES database for NAAA:
    Thrombocytopenia     Anemia
    Human Genome Epidemiology (HuGE) Navigator: NAAA (1 document)

    Export disorders for NAAA gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for NAAA gene, integrated from 9 sources (see all 20):
    (articles sorted by number of sources associating them with NAAA)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Molecular cloning and characterization of a human cDNA and gene encoding a novel acid ceramidase-like protein. (PubMed id 10610717)1, 2, 3, 9 Hong S.-B.... Schuchman E.H. (1999)
    2. A human endogenous long terminal repeat provides a polyadenylation signal to a novel, alternatively spliced transcript in normal placenta. (PubMed id 1446826)1, 2, 3 Goodchild N.L.... Mager D.L. (1992)
    3. Molecular characterization of N-acylethanolamine-hydrolyzing acid amidase, a novel member of the choloylglycine hydrolase family with structural and functional similarity to acid ceramidase. (PubMed id 15655246)1, 2, 9 Tsuboi K.... Ueda N. (2005)
    4. Mass spectrometric characterization of human N-acyleth anolamine-hydrolyzing acid amidase. (PubMed id 22040171)1, 2 West J.M....Makriyannis A. (2012)
    5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    6. Proteolytic activation and glycosylation of N-acylethanolamine-hydrolyzing acid amidase, a lysosomal enzyme involved in the endocannabinoid metabolism. (PubMed id 17980170)1, 9 Zhao L.Y....Ueda N. (2007)
    7. Lack of association of genetic variants in genes of the endocannabinoid system with anorexia nervosa. (PubMed id 19014633)1, 9 Muller T.D....Hinney A. (2008)
    8. Expression and secretion of N-acylethanolamine-hydrolysing acid amidase in human prostate cancer cells. (PubMed id 18806270)1, 9 Wang J....Ueda N. (2008)
    9. Interactions of pathological hallmark proteins: tubul in polymerization promoting protein/p25, beta-amyloid, and alpha-synuclein. (PubMed id 21832049)1 OlA!h J....OvA!di J. (2011)
    10. Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. (PubMed id 19159218)2 Chen R.... Zou H. (2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
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      Query String
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 27163 HGNC: 736 AceView: ASAHL Ensembl:ENSG00000138744 euGenes: HUgn27163
    ECgene: NAAA H-InvDB: NAAA

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for NAAA Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for NAAA gene:
    Search GeneIP for patents involving NAAA

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
    In Situ Hybridization Assays from
    Advanced Cell Diagnostics
    About This Section

     
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