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N6AMT2 Gene

protein-coding GIFtS: 47

GC13M020200

N-6 adenine-specific DNA methyltransferase 2 (putative)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
EC 2.1.1.- ^³
ESP13 ^²
OTTHUMP00000018102 ^²
OTTHUMP00000018103 ^²

Descriptions
N-6 adenine-specific DNA methyltransferase 2 (putative) ^²

External Ids
HGNC: 27351¹
Entrez Gene: 221143²
UniProtKB: Q8WVE0³
Ensembl: ENSG00000150456⁷

Search outside databases for aliases for N6AMT2 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: N6MT2_HUMAN, Q8WVE0

Function: Putative DNA methyltransferase (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 13q12.11 Ensembl cytogenetic band: 13q12.11 HGNC cytogenetic band: 13q12.11

N6AMT2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 13 GeneLoc Exon Structure

GeneLoc location for GC13M020200: (about GC identifiers)

Start:	20,200,870 bp from pter
End:	20,246,088 bp from pter
Size:	45,219 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000013.9 NT_024524.13

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: N6MT2_HUMAN, Q8WVE0 (See protein sequence)

Recommended Name: N(6)-adenine-specific DNA methyltransferase 2

Size: 214 amino acids; 24506 Da

REFSEQ proteins: NP_777588.1

ENSEMBL proteins:

ENSP00000372206 ENSP00000372202

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse Abcam for Recombinant Proteins
	Human Recombinant Proteins from Abnova (LOC221143)

OriGene Purified Recombinant Human Protein: N6AMT2

Antibodies for N6AMT2:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Monoclonal and Polyclonal Antibodies from Abnova (LOC221143)
	Novus Biologicals Antibody for N6AMT2

Assays for N6AMT2:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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Graphical View of Domain Structure for InterPro Entry Q8WVE0

ProtoNet protein and cluster: Q8WVE0

1 Blocks protein family: IPB002052 N-6 Adenine-specific DNA methylase

UniProtKB/Swiss-Prot: N6MT2_HUMAN, Q8WVE0

Similarity: Belongs to the methyltransferase superfamily. AML1 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (N6AMT2)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_174928

Applied Biosystems Silencer® siRNAs for N6AMT2

Sigma-Aldrich siRNA for N6AMT2

Sigma-Aldrich shRNA Panels and shRNA for N6AMT2

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for N6AMT2
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_174928
Myc/DDK tagged cDNA clone in CMV expression vector: NM_174928
untagged cDNA clones in CMV expression vector (see all 2): NM_174928

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_174928

UniProtKB/Swiss-Prot: N6MT2_HUMAN, Q8WVE0

Function: Putative DNA methyltransferase (By similarity)

Enzyme Number (IUBMB): EC 2.1.1.-

1 MGI mutant phenotype (inferred from 1 allele) (MGI details for N6amt2):

no phenotypic analysis

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003676	nucleic acid binding	IEA	--
GO:0008168	methyltransferase activity	IEA	--
GO:0016740	transferase activity	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Interacting protein for N6AMT2 (ENSP00000372202³) via UniProtKB, MINT, and/or STRING

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
NDUFA9	ENSP00000266544³	STRING (score=.801)

About this table

1 Gene Ontology (GO) biological process term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0032259	methylation	IEA	--

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for N6AMT2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (N6AMT2)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_174928

Sigma-Aldrich siRNA for N6AMT2

Sigma-Aldrich shRNA Panels and shRNA for N6AMT2

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_174928

REFSEQ mRNAs for N6AMT2 gene:

NM_174928.1

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_174928

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_174928
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_174928
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_174928

Additional cDNA sequence:

AK316604.1 BC018091.1 CR595343.1 DQ823637.1

6 DOTS entries:

DT.212371 DT.100020686 DT.120764848 DT.95215223 DT.86845889 DT.86849553

24/89 AceView cDNA sequences (see all 89 ):

AA705409 AI669197 CB242633 AW295675 CA312613 BE221713 BM694587 AK055408

AA742306 CA431429 AI085345 AI755175 BX113581 AI638452 AA292535 AA328362

BF445318 CF454974 CR595343 AA284407 CB242296 AW450897 BU730996 CB305681

highest scoring ESTs for N6AMT2:

AI141584 BC018091 BG498110 BG707022 BI600319 BI668445 AA284407 AA292535 AA328362 AA375974

Unigene Cluster for N6AMT2:

N-6 adenine-specific DNA methyltransferase 2 (putative)

Hs.26674 [show with all ESTs]

Unigene Representative Sequence: BG403594

GeneLoc Exon Structure

4 Alternative Splicing Database (ASD) splice patterns (SP) for N6AMT2

ExUns:	1	^	2	^	3	^	4	^	5a	·	5b	^	6a	·	6b
SP1:											-
SP2:			-
SP3:							-				-
SP4:			-				-

About this scheme

ECgene alternative splicing isoforms for N6AMT2

2 Ensembl transcripts including schematic representations:

ENST00000382758 ENST00000382754

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

N6AMT2 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for N6AMT2

¹ / ² / ³

3 probe-sets matching N6AMT2 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
53167_at²^{, 3}	U95-B	1	1.00	1.00	1.00	1.00	AI141584	0.80	1.00	0.91	1
227054_at²^{, 3}	U133-B	1	1.00	1.00	--	--	AI141584	0.80	1.00	0.91	1
227054_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: GAAGAACAGG

SOURCE GeneReport for Unigene cluster: Hs.26674

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for N6AMT2 gene from 5/20 species (see all 20 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	N6AMT2¹	--	N-6 adenine-specific DNA methyltransferase 2 (putative)	86.41(n) 84.36(a)	486048 XM_543173.2 XP_543173.1
chimpanzee (Pan troglodytes)	N6AMT2¹	--	N-6 adenine-specific DNA methyltransferase 2 (putative)	99.38(n) 98.6(a)	452464 XM_001148298.1 XP_001148298.1
cow (Bos taurus)	N6AMT2¹	--	N-6 adenine-specific DNA methyltransferase 2 (putative)	76.48(n) 74.77(a)	783374 XM_001251191.1 XP_001251192.1
rat (Rattus norvegicus)	N6amt2¹	--	N-6 adenine-specific DNA methyltransferase 2 (putative)	80.97(n) 83.49(a)	290279 XM_214202.4 XP_214202.3
mouse (Mus musculus)	N6amt2^{1, 5}	14⁵	N-6 adenine-specific DNA methyltransferase 2 (putative)^{1, 5}	80.82(n)¹ 82.55(a)¹	68043¹ NM_026526.2¹ NP_080802.1¹ AK005312⁵ AK010911⁵ (see all 8)

About this table Species with no ortholog for N6AMT2

ENSEMBL Gene Tree for N6AMT2

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/362 NCBI SNPs in N6AMT2 are shown (see all 362 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 90)

AB	SNP ID	Valid	Chr 13 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs3936081^1,2	A,C,F,H	20246279(+)	`CAAAAA/GAACAA`	1	--	ng3¹		7		EU EA WA NA	524
	rs9509370^1,2	C,F,H	20247422(+)	`GTTCAA/GTAAAT`	1	--	ng3¹		4		EU EA WA	420
	rs12872303^1,2	C,F,H	20246682(+)	`TTGGTC/TGATTG`	1	--	ng3¹		4		EU EA WA	404
	rs12872728^1,2	C,F,H	20246952(+)	`AGCAGC/TTACCA`	1	--	ng3¹		4		EU EA WA	420
	rs12430409^1,2	C,F	20247667(+)	`GATTCA/GTATTT`	1	--	ng3¹ tfbs³		3		NA	140
--	rs9579951^1,2	A	20246291(+)	`AACAAA/CAAAAA`	1	--	ng3¹		0	--	--	--	--
--	rs4770072^1,2	A	20246508(+)	`gactcC/Gctgga`	1	--	ng3¹		0	--	--	--	--
--	rs11549810^1,2	H	20201286(+)	`TCCGGG/TTGTGT`	1	T/N	mis¹ ese³		4		EU EA WA	400
	rs34399030^1,2	F	20204113(+)	`GGTAAG/ATCCAA`	1	D/D	syn¹		1		NA	76
	rs35948132^1,2	F	20204140(+)	`TAGAAA/GATAAA`	1	I/I	syn¹		1		NA	74

About this table

HapMap Linkage Disequilibrium images for N6AMT2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

5 PubMed articles for N6AMT2 gene:

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)^{2, 3} Strausberg R.L....Marra M.A. (2002)
Transcriptome analysis of a cDNA library from adult human epididymis. (PubMed id 18390568)³ Li J.Y.... Zhang Y.L. (2008)
The DNA sequence and analysis of human chromosome 13. (PubMed id 15057823)⁴ Dunham A.... Ross M.T. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)⁴ Gerhard D.S....Malek J. (2004)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)³ Ota T....Sugano S. (2004)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 221143	HGNC: 27351	AceView: LOC221143	Ensembl:ENSG00000150456	euGenes: HUgn221143
ECgene: N6AMT2	H-InvDB: N6AMT2

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Licensable Technologies for N6AMT2
(Available from WIS Yeda, Salk, Tufts)

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(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for N6AMT2:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for N6AMT2-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for N6AMT2		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for N6AMT2		Browse small molecules at Sigma-Aldrich
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	Recombinant & Natural Proteins

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	Tagged/untagged cDNA clones
	Validated SYBR primer pairs
	Purified proteins from HEK293T cells
	Western blot validated antibodies
	shRNA in GFP-retroviral vector

Search Tocris compounds for N6AMT2

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

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N6AMT2 Gene

protein-coding GIFtS: 47

Aliases &
Descriptions
for N6AMT2

Summaries
for N6AMT2

Genomic Location
for N6AMT2

Proteins
for N6AMT2

Protein Domains/
Families
for N6AMT2

Gene Function
for N6AMT2

Pathways & Interactions
for N6AMT2

Drugs & Compounds
for N6AMT2

Transcripts
for N6AMT2

Expression
for N6AMT2

Orthologs
for N6AMT2

Paralogs
for N6AMT2

SNPs/Variants
for N6AMT2

Disorders & Mutations
for N6AMT2

Medical News
for N6AMT2

Publications
for N6AMT2

Search for N6AMT2

Genome Databases showing N6AMT2

Other Databases showing N6AMT2

Specialized Databases showing N6AMT2

Licensable Technologies
for N6AMT2

Services
for N6AMT2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

N6AMT2 Gene

protein-coding GIFtS: 47

Aliases & Descriptionsfor N6AMT2

Summariesfor N6AMT2

Genomic Locationfor N6AMT2

Proteinsfor N6AMT2

Protein Domains/Familiesfor N6AMT2

Gene Functionfor N6AMT2

Pathways & Interactionsfor N6AMT2

Drugs & Compoundsfor N6AMT2

Transcriptsfor N6AMT2

Expression for N6AMT2

Orthologsfor N6AMT2

Paralogsfor N6AMT2

SNPs/Variantsfor N6AMT2

Disorders & Mutationsfor N6AMT2

Medical Newsfor N6AMT2

Publicationsfor N6AMT2

Search for N6AMT2

Genome Databases showing N6AMT2

Other Databases showing N6AMT2

Specialized Databases showing N6AMT2

Licensable Technologiesfor N6AMT2

Servicesfor N6AMT2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for N6AMT2

Summaries
for N6AMT2

Genomic Location
for N6AMT2

Proteins
for N6AMT2

Protein Domains/
Families
for N6AMT2

Gene Function
for N6AMT2

Pathways & Interactions
for N6AMT2

Drugs & Compounds
for N6AMT2

Transcripts
for N6AMT2

Expression
for N6AMT2

Orthologs
for N6AMT2

Paralogs
for N6AMT2

SNPs/Variants
for N6AMT2

Disorders & Mutations
for N6AMT2

Medical News
for N6AMT2

Publications
for N6AMT2

Licensable Technologies
for N6AMT2

Services
for N6AMT2