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N4BP3 Gene

protein-coding GIFtS: 43

GC05P177473

Nedd4 binding protein 3
symbol from the EntrezGene Database
NOT approved by the HUGO Gene Nomenclature Committee (HGNC).

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
KIAA0341 ^², ³

Descriptions
Nedd4 binding protein 3 ^²

External Ids
Entrez Gene: 23138²
UniProtKB: O15049³
Ensembl: ENSG00000145911⁷

Search outside databases for aliases for N4BP3 gene

Previous GC identifers: GC05P177695 GC05P178427 GC05P177608 GC05P177654

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the N4BP3 gene

Entrez Gene cytogenetic band: 5q35.3 Ensembl cytogenetic band: 5q35.3

N4BP3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 5 GeneLoc Exon Structure

GeneLoc location for GC05P177473: (about GC identifiers)

Start:	177,473,162 bp from pter
End:	177,485,713 bp from pter
Size:	12,552 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000005.8 NT_023133.12

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: N4BP3_HUMAN, O15049 (See protein sequence)

Recommended Name: NEDD4-binding protein 3

Size: 544 amino acids; 60470 Da

Subunit: Binds NEDD4

Subcellular location: Cytoplasmic vesicle membrane; Peripheral membrane protein

Secondary accessions: Q7Z6I3

Post-translational modifications:

View phosphorylation sites using PhosphoSite²

REFSEQ proteins: NP_055926.1

ENSEMBL proteins:

ENSP00000274605

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
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	Browse Abnova for Human Recombinant Proteins

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

4 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005737	cytoplasm	IEA	--
GO:0016020	membrane	IEA	--
GO:0030659	cytoplasmic vesicle membrane	IEA	--
GO:0031410	cytoplasmic vesicle	IEA	--

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Antibodies for N4BP3:

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	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for N4BP3

Assays for N4BP3:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
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	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry O15049

ProtoNet protein and cluster: O15049

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (N4BP3)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_015111

Applied Biosystems Silencer® siRNAs for N4BP3

Sigma-Aldrich siRNA for N4BP3

Sigma-Aldrich shRNA for N4BP3

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_015111
Myc/DDK tagged cDNA clone in CMV expression vector: NM_015111
untagged cDNA clones in CMV expression vector (see all 2): NM_015111

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_015111

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

1 Interacting protein for N4BP3 (O15049²) via UniProtKB, MINT, and/or STRING
Interactant Interaction Details
GeneCard External ID(s)
-- P46935² MINT-13747 MINT-13745 MINT-13746
About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for N4BP3

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (N4BP3)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_015111

Sigma-Aldrich siRNA for N4BP3

Sigma-Aldrich shRNA for N4BP3

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_015111

REFSEQ mRNAs for N4BP3 gene:

NM_015111.1

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_015111

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_015111
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_015111
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_015111

Additional cDNA sequence:

AB002339.1 AK127224.1 AK295663.1 BC027715.2 BC053323.1 CR613906.1

4 DOTS entries:

DT.407217 DT.99951796 DT.99939699 DT.100689194

24/40 AceView cDNA sequences (see all 40 ):

BC053323 CA946541 BX391050 BC027715 CR613906 BU538610 BU557397 AA372485

BE791648 BU429239 AB002339 BF310040 AU126244 CD673795 AW083116 BF309170

BX505754 BE378392 AL583320 AW139448 CB989360 AL551173 AL551198 AA829496

highest scoring ESTs for N4BP3:

AB002339 AA361587 AA372485 AA829496 AI269677 AK127224 AL583267 AU126244 AW083116 AW139448

Unigene Cluster for N4BP3:

Nedd4 binding protein 3

Hs.101761 [show with all ESTs]

Unigene Representative Sequence: NM_015111

GeneLoc Exon Structure

2 Alternative Splicing Database (ASD) splice patterns (SP) for N4BP3

ExUns:	1	^	2a	·	2b	^	3	^	4	^	5
SP1:
SP2:

About this scheme

1 Ensembl transcript including schematic representation:

ENST00000274605

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

N4BP3 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for N4BP3

¹ / ² / ³

3 probe-sets matching N4BP3 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
35057_at²^{, 3}	U95-A	1	1.00	1.00	1.00	1.00	AB002339	1.00	1.00	1.00	1
214775_at²^{, 3}	U133-A	1	1.00	1.00	--	--	AW139448	0.80	1.00	0.91	1
214775_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: TTTTCTAAAA

SOURCE GeneReport for Unigene cluster: Hs.101761
Expression variation in blood from EXPOLDB for N4BP3

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for N4BP3 gene from 5/7 species (see all 7 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	LOC481446¹	--	similar to Nedd4-binding protein 3 (N4BP3)	88.17(n) 90.57(a)	481446 XM_538567.2 XP_538567.2
chimpanzee (Pan troglodytes)	LOC471763¹	--	Nedd4 binding protein 3	99.33(n) 99.45(a)	471763 XM_001146257.1 XP_001146257.1
cow (Bos taurus)	LOC513032¹	--	similar to NEDD4-binding protein 3 (N4BP3)	89.9(n) 92.61(a)	513032 XM_590649.3 XP_590649.2
rat (Rattus norvegicus)	N4bp3¹	--	Nedd4 binding protein 3	87.15(n) 92.18(a)	303112 NM_001033893.1 NP_001029065.1
mouse (Mus musculus)	C330016O10Rik^{1, 5}	11⁵	RIKEN cDNA C330016O10 gene^{1, 5}	86.72(n)¹ 91.99(a)¹	212706¹ NM_145974.3¹ NP_666086.2¹ AI854061⁵ AK049249⁵ (see all 16)

About this table Species with no ortholog for N4BP3

ENSEMBL Gene Tree for N4BP3

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for N4BP3 gene

LZTS1² ENSG00000088899² LZTS2²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/66 NCBI SNPs in N4BP3 are shown (see all 66 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 22)

AB	SNP ID	Valid	Chr 5 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
--	rs13180226^1,2	A,C,F,H	177482895(+)	`TGGACG/AGGCTG`	1	--	ut3¹ ese³		4		EU EA WA	412
	rs3812075^1,2	A,C,F,H	177483775(-)	`GGTGGG/ACCCTG`	1	--	ut3¹ ese³		4		EU EA WA	408
	rs3812080^1,2	C,F,H	177482097(-)	`TGGCCA/GTGGGA`	1	--	ut3¹ ese³		5		EA EU WA	1920
	rs3812073^1,2	C,F,H	177484591(-)	`GCTGTG/AGTGAA`	1	--	ut3¹ ese³		5		EA EU WA	1910
	rs3812079^1,2	A,C,F	177483082(-)	`TGAGGG/TGGGGC`	1	--	ut3¹ ese³		1		EA	1498
--	rs7701678^1,2	A,C	177485714(+)	`acaagC/Tggtgc`	1	--	ng3¹		0	--	--	--	--
	rs3812081^1,2	A,C	177481940(-)	`AAGGCC/TCCAGC`	1	--	ut3¹		0	--	--	--	--
	rs3812074^1,2	A,C	177483782(-)	`ATACCA/GAGGTG`	1	--	ut3¹		0	--	--	--	--
--	rs6601215^1,2	A,C	177482698(+)	`CTCCCA/GGATCC`	1	--	ut3¹ ese³		0	--	--	--	--
--	rs7735932^1,2	A,C	177483514(+)	`CCCATA/GCAGGC`	1	--	ut3¹ ese³		0	--	--	--	--

About this table

HapMap Linkage Disequilibrium images for N4BP3 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

5 PubMed articles for N4BP3 gene:

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase. (PubMed id 11717310)^{3, 4} Murillas R....Kuehn M.R. (2002)
Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. (PubMed id 9205841)^{3, 4} Nagase T....Ohara O. (1997)
A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648)⁴ Dephoure N.... Gygi S.P. (2008)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 23138

AceView: N4BP3

Ensembl:ENSG00000145911

euGenes: HUgn23138

(According to HUGE)
About This Section

HUGE: KIAA0341

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for N4BP3
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for N4BP3:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for N4BP3-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for N4BP3		Browse antibodies at Sigma-Aldrich
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N4BP3 Gene

protein-coding GIFtS: 43

Aliases &
Descriptions
for N4BP3

Summaries
for N4BP3

Genomic Location
for N4BP3

Proteins
for N4BP3

Protein Domains/
Families
for N4BP3

Gene Function
for N4BP3

Pathways & Interactions
for N4BP3

Drugs & Compounds
for N4BP3

Transcripts
for N4BP3

Expression
for N4BP3

Orthologs
for N4BP3

Paralogs
for N4BP3

SNPs/Variants
for N4BP3

Disorders & Mutations
for N4BP3

Medical News
for N4BP3

Publications
for N4BP3

Search for N4BP3

Genome Databases showing N4BP3

Other Databases showing N4BP3

Specialized Databases showing N4BP3

Licensable Technologies
for N4BP3

Services
for N4BP3

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

N4BP3 Gene

protein-coding GIFtS: 43

Aliases & Descriptionsfor N4BP3

Summariesfor N4BP3

Genomic Locationfor N4BP3

Proteinsfor N4BP3

Protein Domains/Familiesfor N4BP3

Gene Functionfor N4BP3

Pathways & Interactionsfor N4BP3

Drugs & Compoundsfor N4BP3

Transcriptsfor N4BP3

Expression for N4BP3

Orthologsfor N4BP3

Paralogsfor N4BP3

SNPs/Variantsfor N4BP3

Disorders & Mutationsfor N4BP3

Medical Newsfor N4BP3

Publicationsfor N4BP3

Search for N4BP3

Genome Databases showing N4BP3

Other Databases showing N4BP3

Specialized Databases showing N4BP3

Licensable Technologiesfor N4BP3

Servicesfor N4BP3

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for N4BP3

Summaries
for N4BP3

Genomic Location
for N4BP3

Proteins
for N4BP3

Protein Domains/
Families
for N4BP3

Gene Function
for N4BP3

Pathways & Interactions
for N4BP3

Drugs & Compounds
for N4BP3

Transcripts
for N4BP3

Expression
for N4BP3

Orthologs
for N4BP3

Paralogs
for N4BP3

SNPs/Variants
for N4BP3

Disorders & Mutations
for N4BP3

Medical News
for N4BP3

Publications
for N4BP3

Licensable Technologies
for N4BP3

Services
for N4BP3