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N4BP2L2 Gene

protein-coding   GIFtS: 43

GC13M031891
NEDD4 binding protein 2-like 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
92M18.3 2
CG005 1, 2, 3
CG016 2
FLJ36195 2
FLJ41089 2
FLJ43077 2
OTTHUMP00000018230 2
OTTHUMP00000042409 2
OTTHUMP00000042420 2
PFAAP5 1, 2, 3
Descriptions
92M18.3 (novel protein) 2
NEDD4 binding protein 2-like 2 2
Phosphonoformate immuno-associated protein 5 2, 3
protein from BCRA2 region 2
External Ids
HGNC: 269161
Entrez Gene: 104432
UniProtKB: Q928023
Ensembl: ENSG000001396177
Search outside databases for aliases for N4BP2L2 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

  --
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 13q13.1   Ensembl cytogenetic band:  13q13.1   HGNC cytogenetic band: 13q13.1

N4BP2L2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 13         GeneLoc Exon Structure

GeneLoc location for GC13M031891:     (about GC identifiers)

Start:
31,891,545 bp from pter
End:
32,010,936 bp from pter
Size:
119,392 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000013.9  NT_024524.13  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: N42L2_HUMAN, Q92802 (See protein sequence)
Recommended Name: NEDD4-binding protein 2-like 2  
Size: 583 amino acids; 67459 Da
Secondary accessions: A3KME8
Alternative splicing: 2 isoforms:  Q92802-1   Q92802-2   

Post-translational modifications:

  • Phosphorylated upon DNA damage, probably by ATM or ATR1
  • View phosphorylation sites using PhosphoSite2 (PFAAP5)


  • REFSEQ proteins (2 alternative transcripts): 
    NP_055702.1  NP_149102.3  


    ENSEMBL proteins: 
    ENSP00000267068 ENSP00000369464 ENSP00000350104 ENSP00000382328 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
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    Browse Abcam for Recombinant Proteins
    Human Recombinant Proteins from Abnova (PFAAP5)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005575 cellular_component ND--
    About this table

    Antibodies for N4BP2L2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for N4BP2L2

    Assays for N4BP2L2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ProtoNet protein and cluster: Q92802

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (N4BP2L2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_033111

                  Applied Biosystems Silencer® siRNAs for N4BP2L2

                  Sigma-Aldrich siRNA for N4BP2L2  
                         Sigma-Aldrich shRNA for N4BP2L2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for N4BP2L2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_014887
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_014887
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_014887 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_033111

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003674 molecular_function ND--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    1 Interacting protein for N4BP2L2 (ENSP000003501043) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TMEM87BENSP000002832063STRING (score=.7)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008150 biological_process ND--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for N4BP2L2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (N4BP2L2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_033111

                  Sigma-Aldrich siRNA for N4BP2L2  
                         Sigma-Aldrich shRNA for N4BP2L2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_014887  NM_033111  

    REFSEQ mRNAs for N4BP2L2 gene (2 alternative transcripts): 

    NM_014887.2   NM_033111.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_014887  NM_033111  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_014887
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_014887
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_014887 

    Additional cDNA sequence: 

    AF530063.1 AK093514.1 AK123084.1 AK298546.1 AK310280.1 AK312924.1 AL049783.1 AL049802.1 
    BC010643.1 BC131631.1 BT007172.1 CR596312.1 CR601845.1 U50529.1 U50532.1 U50535.1 
    Z36834.1 

    24/45 DOTS entries (see all 45 ):

    DT.317482  DT.91754565  DT.412749  DT.92454943  DT.100797278  DT.120793871  DT.100777575  DT.95325227 
    DT.100797284  DT.109140  DT.92454928  DT.100797290  DT.100797280  DT.100797279  DT.120793931  DT.95368042 
    DT.100797275  DT.92454938  DT.97847541  DT.120793822  DT.100655678  DT.91745197  DT.92420379  DT.100737250 

    24/449 AceView cDNA sequences (see all 449 ):

    AW236098 BE645120 CD722084 AU117279 BQ267843 AA890530 AK093514 CB240991 
    BE502838 AL049788 BP379646 AI338989 AL596913 BM543104 AW009076 BM670744 
    NM_052818 BM456347 BX483989 BC010643 AI536840 CA840895 BM667255 AA488718 

    Unigene Cluster for N4BP2L2:

    NEDD4 binding protein 2-like 2
    Hs.507680  [show with all ESTs]
    Unigene Representative Sequence: NM_014887


    GeneLoc Exon Structure

    5/14 Alternative Splicing Database (ASD) splice patterns (SP) for N4BP2L2 (see all 14 )

    ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b ^ 3a · 3b · 3c ^ 4 ^ 5a · 5b · 5c ^ 6 ^ 7 ^ 8a · 8b ^ 9a · 9b · 9c · 9d ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^
    SP1:                                                                                                                                                            
    SP2:                                                                                                                                      -     -           -   
    SP3:                                                                          -           -     -                                                               
    SP4:                                                                    -     -           -     -                 -     -     -     -     -     -     -     -   
    SP5:                                                                                                              -     -     -     -     -     -           -   

    ExUns: 14 ^ 15 ^ 16a · 16b · 16c · 16d · 16e ^ 17 ^ 18 ^ 19a · 19b
    SP1:                                                                  
    SP2:        -                                                         
    SP3:                                                                  
    SP4:  -     -                                                         
    SP5:  -     -                                                         

    About this scheme

    ECgene alternative splicing isoforms for N4BP2L2

    4 Ensembl transcripts including schematic representations:
    ENST00000267068  ENST00000380121  ENST00000357505  ENST00000399396  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    N4BP2L2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for N4BP2L2

    1 / 2

    14 probe-sets matching N4BP2L2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    33827_at2 U95-A 1 1.00 1.00 0.15 1.25 -- -- -- -- --
    34459_at2 U95-A 1 1.00 1.00 0.55 1.30 -- -- -- -- --

    1531_at*2 U95-A 1 1.00 1.00 0.67 0.62 -- -- -- -- --

    1532_g_at*2 U95-A 1 1.00 1.00 0.26 0.86 -- -- -- -- --

    214748_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

    202259_s_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

    202258_s_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

    214753_at*2 U133-A 1 0.73 1.00 -- -- -- -- -- -- --

    235547_at*2 U133-B NULL 0.00 0.00 -- -- -- -- -- -- --

    214748_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    202259_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    202258_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    214753_at*2 U133Plus2 1 0.73 1.00 -- -- -- -- -- -- --

    235547_at*2 U133Plus2 NULL 0.00 0.00 -- -- -- -- -- -- --
    GeneDecks  N4BP2L2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GTGTGTCTCG

    SOURCE GeneReport for Unigene cluster: Hs.507680
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for N4BP2L2 gene from 4 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    cow
    (Bos taurus)
    N4BP2L21   -- NEDD4 binding protein 2-like 2 86.27(n)
    76.12(a)
    541201  XM_615015.3  XP_615015.2 
    rat
    (Rattus norvegicus)
    N4bp2l21   -- NEDD4 binding protein 2-like 2 78.8(n)
    67.42(a)
    288416  NM_001005533.1  NP_001005533.1 
    mouse
    (Mus musculus)
    N4bp2l21, 5 55
    NEDD4 binding protein 2-like 21, 5 78.15(n)1
    60.93(a)1
    3816951  NM_201369.21  NP_958757.11 
     AA0975385  AK0192305  (see all 20)
    chicken
    (Gallus gallus)
    RCJMB04_33d71   -- NEDD4 binding protein 2-like 2 59.3(n)
    43.37(a)
    418912  NM_001012828.1  NP_001012846.1 
    About this table        Species with no ortholog for N4BP2L2

    ENSEMBL Gene Tree for N4BP2L2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/507 NCBI SNPs in N4BP2L2 are shown (see all 507 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 63)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 13 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs93371,2
    A,C,F,H,O31989241(+) TATTAC/GAATCA 2 -- int1 ut3119Minor allele frequency- N:0.00MN NS EA NA EU WA 1520
    rs2076201,2
    A,C,F,H,O32011403(+) TACTGC/GGGCGC 2 -- ng3110Minor allele frequency- G:0.30EA NA MN EU WA 1058
    --
    rs37423181,2
    A,C,F,H31915043(+) GTTTAT/CTCTCC 1 S/N mis1 trp314Minor allele frequency- C:0.20EA EU WA NA 2230
    --
    rs170775681,2
    C,F,H31914660(+) TTCTTC/TCCATC 1 E/K mis112Minor allele frequency- T:0.06NA EU EA WA 1042
    --
    rs170775691,2
    C,F,H31915158(+) GATACG/TACTCT 1 R/S mis1 ese312Minor allele frequency- T:0.05NA EU EA WA 1052
    --
    rs345398881,2
    C,F31914603(+) GGAACG/TGTAGT 1 R/S mis15Minor allele frequency- T:0.07NA EU EA WA 494
    rs340624611,2
    C,F32008351(+) AGGATA/GTACTG 2 Y/H mis1 int15Minor allele frequency- G:0.07NA EU EA WA 494
    --
    rs2076221,2
    A,C32012557(+) TTTTTC/TCCCAC 2 -- ng31 trp30--------
    rs170776271,2
    F,H31988603(+) GTTGAT/CAGCAG 2 -- int1 ng517Minor allele frequency- C:0.01NA EU EA WA 540
    --
    rs351088101,2
    C,F31915937(+) TGTTGT/ACTAAT 1 V/D mis15Minor allele frequency- A:0.05NA EU EA WA 488
    About this table

    HapMap Linkage Disequilibrium images for N4BP2L2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    8 PubMed articles for N4BP2L2 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 10443 HGNC: 26916 AceView: PFAAP5 Ensembl:ENSG00000139617 euGenes: HUgn10443
    ECgene: N4BP2L2 H-InvDB: N4BP2L2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for N4BP2L2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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