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N4BP2 Gene

protein-coding   GIFtS: 54

GC04P039734
NEDD4 binding protein 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
B3BP 1, 2, 3
EC 3.-.-.- 3
FLJ10680 2
KIAA1413 2, 3
OTTHUMP00000158757 2
Descriptions
BCL-3 binding protein 2
BCL-3-binding protein 3
NEDD4 binding protein 2 2
External Ids
HGNC: 298511
Entrez Gene: 557282
UniProtKB: Q86UW63
Ensembl: ENSG000000781777
Search outside databases for aliases for N4BP2 gene

Previous GC identifers: GC04P040068 GC04P040266 GC04P039893 GC04P039996 GC04P039972 GC04P039897

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for N4BP2:
This gene encodes a protein containing a polynucleotide kinase domain (PNK) near the N-terminal
region, and a Small MutS Related (Smr) domain near the C-terminal region. The encoded protein can
bind to both B-cell leukemia/lymphoma 3 (BCL-3) and neural precursor cell expressed,
developmentally downregulated 4, (Nedd4) proteins. This protein binds and hydrolyzes ATP, may
function as a 5'-polynucleotide kinase, and has the capacity to be a ubiquitylation
substrate. This protein may play a role in transcription-coupled DNA repair or genetic
recombination. [provided by RefSeq]

UniProtKB/Swiss-Prot: N4BP2_HUMAN, Q86UW6
Function: Has 5'-polynucleotide kinase and nicking endonuclease activity. May play a role in DNA
repair or recombination

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the N4BP2 gene  

Entrez Gene cytogenetic band: 4p14   Ensembl cytogenetic band:  4p14   HGNC cytogenetic band: 4p14

N4BP2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P039734:     (about GC identifiers)

Start:
39,734,919 bp from pter
End:
39,836,267 bp from pter
Size:
101,349 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000004.10  NT_016297.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: N4BP2_HUMAN, Q86UW6 (See protein sequence)
Recommended Name: NEDD4-binding protein 2  
Size: 1770 amino acids; 198801 Da
Subunit: Binds NEDD4 (By similarity). Binds BCL3 and CREBBP
Subcellular location: Cytoplasm (By similarity)
PDB structures from and Proteopedia :
2D9I (3D)    2VKC (3D)    3BHB (3D)    
Secondary accessions: A0AVR3 Q9NVK2 Q9P2D4
Alternative splicing: 2 isoforms:  Q86UW6-1   Q86UW6-2   

Post-translational modifications:

  • Ubiquitinated; this targets the protein for degradation by the proteasome (By similarity)1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_060647.2  

    ENSEMBL proteins: 
    ENSP00000371225 ENSP00000261435 


    Human Recombinant Proteins 
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    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (N4BP2)
    Human Recombinant Proteins from Abnova (N4BP2)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm IEA--
    About this table

    Antibodies for N4BP2: 
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    Sigma-Aldrich Antibodies for N4BP2
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    Antibodies from Abcam (N4BP2), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (N4BP2)
    Novus Biologicals Antibodies for N4BP2

    Assays for N4BP2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR002625 Smr/MutS2_C
     IPR003892 CUE
     IPR013899 DUF1771


       GeneDecks  N4BP2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q86UW6

    ProtoNet protein and cluster: Q86UW6

    2 Blocks protein families:
    IPB002625 Smr protein/MutS2 C-terminal
    IPB003892 Ubiquitin system component Cue


    UniProtKB/Swiss-Prot: N4BP2_HUMAN, Q86UW6
    Domain: The Smr domain has nicking endonuclease activity, but no significant double strand cleavage
    or exonuclease activity
    Similarity: Contains 1 CUE domain
    Similarity: Contains 1 Smr domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (N4BP2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (N4BP2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018177

                  Applied Biosystems Silencer® siRNAs for N4BP2

                  Sigma-Aldrich siRNA for N4BP2  
                         Sigma-Aldrich shRNA for N4BP2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_018177
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_018177
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_018177 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_018177

    UniProtKB/Swiss-Prot: N4BP2_HUMAN, Q86UW6
    Function: Has 5'-polynucleotide kinase and nicking endonuclease activity. May play a role in DNA
    repair or recombination
    Enzyme Number (IUBMB): EC 3.-.-.- 

    5/6 Gene Ontology (GO) molecular function terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0004519 endonuclease activity IDA12730195
    GO:0005515 protein binding IPI12730195
    GO:0005524 ATP binding IDA12730195
    GO:0016787 hydrolase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  N4BP2 


    2 Interacting proteins for N4BP2 (ENSP000002614353) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    BCL3ENSP000001642273STRING (score=.978)
    NEDD4ENSP000003455303STRING (score=.842)
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for N4BP2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (N4BP2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (N4BP2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018177

                  Sigma-Aldrich siRNA for N4BP2  
                         Sigma-Aldrich shRNA for N4BP2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_018177  

    REFSEQ mRNAs for N4BP2 gene: 

    NM_018177.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_018177  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_018177
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_018177
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_018177 

    Additional cDNA sequence: 

    AB037834.1 AB371584.1 AK001542.1 AY267013.1 BC126466.1 

    6 DOTS entries:

    DT.410570  DT.95070779  DT.99962520  DT.309401  DT.95303939  DT.95303940 

    24/80 AceView cDNA sequences (see all 80 ):

    AB037834 BQ008834 BM804242 BE856543 BE550411 H03459 AI278279 AI973227 
    NM_018177 AA598849 F05693 AK001542 R93882 BU161412 BX503904 BE887093 
    BM468557 CD642915 CD522214 R57027 BF107284 BX409876 BM460199 AI831502 

    highest scoring ESTs for N4BP2:

    AA699681 AB037834 AI393829 AI655142 BG389188 BM791313 BX503904 N70425 R54310 W93336 

    Unigene Cluster for N4BP2:

    NEDD4 binding protein 2
    Hs.391463  [show with all ESTs]
    Unigene Representative Sequence: AB371584


    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for N4BP2 (see all 6 )

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14a · 14b ^ 15 ^ 16 ^ 17a · 17b ^ 18 ^ 19 ^ 20
    SP1:              -     -                                                                                                                                 
    SP2:                                                                                                                                                      
    SP3:        -                                                                                                                                             
    SP4:                                                                                                                                                      
    SP5:                                                                                                                                                      

    About this scheme

    ECgene alternative splicing isoforms for N4BP2

    2 Ensembl transcripts including schematic representations:
    ENST00000381804  ENST00000261435  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    N4BP2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for N4BP2

    1 / 2 / 3

    8 probe-sets matching N4BP2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    54845_at2, 3 U95-C 1 1.00 1.00 0.71 1.41 AI831502 0.20 1.00 0.72 1

    48376_at2, 3 U95-B 1 1.00 1.00 0.11 0.24 AI393829 0.80 1.00 0.91 1

    77613_at2, 3 U95-E 1 1.00 1.00 0.73 0.64 R54310 0.80 1.00 0.91 1

    52503_at2, 3 U95-C 1 1.00 1.00 0.77 0.35 AA699681 1.00 1.00 1.00 1

    231996_at2, 3 U133-B 1 1.00 1.00 -- -- AB037834 0.80 1.00 0.91 1

    228242_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

    231996_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    228242_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  N4BP2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GTGAATCCCC

    SOURCE GeneReport for Unigene cluster: Hs.391463
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for N4BP2 gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    N4BP21   -- NEDD4 binding protein 2 88.4(n)
    82.69(a)
    479101  XM_536248.2  XP_536248.2 
    chimpanzee
    (Pan troglodytes)
    N4BP21   -- Nedd4 binding protein 2 99.66(n)
    99.6(a)
    471175  XM_526557.2  XP_526557.2 
    cow
    (Bos taurus)
    N4BP21   -- NEDD4 binding protein 2 86.9(n)
    81.41(a)
    539533  XM_587938.3  XP_587938.3 
    rat
    (Rattus norvegicus)
    N4bp21   -- NEDD4 binding protein 2 79.18(n)
    72.77(a)
    305342  XM_223405.4  XP_223405.4 
    mouse
    (Mus musculus)
    N4bp21, 5 55
    NEDD4 binding protein 21, 5 78.56(n)1
    72.18(a)1
    3337891  NM_001024917.11  NP_001020088.11 
     AA6931145  AI1555475  (see all 18)
    About this table        Species with no ortholog for N4BP2

    ENSEMBL Gene Tree for N4BP2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for N4BP2 gene
    N4BP2L12  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/300 NCBI SNPs in N4BP2 are shown (see all 300 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 76)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 4 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs8132301,2
    A,C,F,H,O39774010(+) TATGGT/GTGAAA 1 -- ng515Minor allele frequency- G:0.35MN EU EA WA 602
    rs7940011,2
    A,C,F,H,O39797957(+) GAGACA/GATGAA 1 D/N mis1 ese317Minor allele frequency- N:0.01NA EA MN EU WA 1414
    rs49749611,2
    A,C,F,H39773952(+) gaaaaT/Cagact 1 -- ng518Minor allele frequency- C:0.34EU EA WA NA 780
    rs175116681,2
    C,F,H39780162(+) ACAAAG/TTTTCG 1 S/I mis116Minor allele frequency- T:0.06NA EU EA WA 1472
    rs22713951,2
    C,F,H39815071(-) GCCAGT/CATGAG 1 A/T mis1 ese313Minor allele frequency- C:0.35EA EU WA 2526
    rs100141701,2
    C,F,H39780446(+) AAAATA/GTGAAT 1 M/V mis18Minor allele frequency- G:0.05EU EA WA 838
    rs68424351,2
    C39833608(+) TATAAC/TGTGGT 1 -- ng310--------
    --
    rs100027781,2
    C39833236(+) tccccA/Gtctct 1 -- ut31 ese30--------
    rs32107321,2
    H39815044(+) TTGTTC/AACCAA 1 N/H mis1 ese35Minor allele frequency- A:0.00MN EU EA WA 588
    rs11283721,2
    C,F,H39795896(+) ATTCAT/CGGGGT 1 H/H syn18Minor allele frequency- C:0.09NA MN EU EA WA 732
    About this table

    HapMap Linkage Disequilibrium images for N4BP2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/11 PubMed articles for N4BP2 gene (see all 11 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 55728 HGNC: 29851 AceView: N4BP2 Ensembl:ENSG00000078177 euGenes: HUgn55728
    ECgene: N4BP2 H-InvDB: N4BP2
    (According to HUGE)
    About This Section

    HUGE: KIAA1413
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for N4BP2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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