N4BP2 Gene
protein-coding GIFtS: 48
GCID: GC04P040058
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|
NEDD4 binding protein 2
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Aliases
for N4BP2 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| NEDD4 Binding Protein 21 2 | | B3BP1 2 3 | | BCL-3-Binding Protein2 3 | | NEDD4-Binding Protein 22 | | EC 3.-.-.-3 | | KIAA14133 |
Export aliases for N4BP2 gene to outside databasesPrevious GC identifers: GC04P040068 GC04P040266 GC04P039893 GC04P039996 GC04P039972 GC04P039897 GC04P039734 GC04P039775 GC04P039381 |
Summaries
for N4BP2 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for N4BP2:
This gene encodes a protein containing a polynucleotide kinase domain (PNK) near the N-terminal region, and a SmallMutS Related (Smr) domain near the C-terminal region. The encoded protein can bind to both B-cell leukemia/lymphoma 3(BCL-3) and neural precursor cell expressed, developmentally downregulated 4, (Nedd4) proteins. This protein binds andhydrolyzes ATP, may function as a 5'-polynucleotide kinase, and has the capacity to be a ubiquitylation substrate.This protein may play a role in transcription-coupled DNA repair or genetic recombination. (provided by RefSeq, Jul2008)
UniProtKB/Swiss-Prot: N4BP2_HUMAN, Q86UW6Function: Has 5'-polynucleotide kinase and nicking endonuclease activity. May play a role in DNA repair orrecombination
|
Genomic Views
for N4BP2 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000004.11 NC_018915.1 NT_016297.16
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the N4BP2 gene promoter:
Nkx3-1 Nkx3-1 v4 Pax-6 Nkx3-1 v1 Nkx2-5 LCR-F1 Nkx3-1 v2 FOXO1a Nkx3-1 v3 FOXO1
Other transcription factors
Search SABiosciences Chromatin IP Primers for N4BP2
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat N4BP2 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 4p14 Ensembl cytogenetic band: 4p14 HGNC cytogenetic band: 4p14N4BP2 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 4 GeneLoc Exon Structure GeneLoc location for GC04P040058: view genomic region
(about GC identifiers)
Start:
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40,058,446 bp from pter |
End:
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40,159,872 bp from pter |
Size:
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101,427 bases |
Orientation:
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plus strand |
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Proteins
for N4BP2 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: N4BP2_HUMAN, Q86UW6 (See
protein sequence)Recommended Name: NEDD4-binding protein 2 Size: 1770 amino acids; 198801 Da
Subunit: Binds NEDD4 (By similarity). Binds BCL3 and CREBBP
Subcellular location: Cytoplasm (By similarity)
4 PDB 3D structures from  and Proteopedia  for N4BP2:2D9I (3D)
2VKC (3D)
3BHB (3D)
3FAU (3D)
Secondary accessions: A0AVR3 Q9NVK2 Q9P2D4Alternative splicing: 2 isoforms: Q86UW6-1 Q86UW6-2 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for N4BP2: NX_Q86UW6
Post-translational modifications:
Ubiquitinated; this targets the protein for degradation by the proteasome (By similarity)1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q86UW6
N4BP2 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_060647.2
ENSEMBL proteins: ENSP00000261435 ENSP00000422436 ENSP00000422057 ENSP00000426430
Human Recombinant Protein Products:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table
N4BP2 for ontologies About GeneDecksing
N4BP2 Antibody Products:
Assay Products for N4BP2: |
Protein
Domains /
Families
for N4BP2 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
N4BP2 for domains About GeneDecksing
4 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q86UW6ProtoNet protein and cluster: Q86UW6 2 Blocks protein families: IPB002625 Smr protein/MutS2 C-terminal IPB003892 Ubiquitin system component Cue
UniProtKB/Swiss-Prot: N4BP2_HUMAN, Q86UW6Domain: The Smr domain has nicking endonuclease activity, but no significant double strand cleavage or exonucleaseactivitySimilarity: Contains 1 CUE domainSimilarity: Contains 1 Smr domain |
Function
for N4BP2 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: N4BP2_HUMAN, Q86UW6Function: Has 5'-polynucleotide kinase and nicking endonuclease activity. May play a role in DNA repair orrecombinationEnzyme Number (IUBMB): EC 3.-.-.-1
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for N4BP2 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for N4BP2 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for N4BP2 |
Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view): About this table
N4BP2 for ontologies About GeneDecksing
|
Pathways & Interactions
for N4BP2 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for N4BP2
STRING Interaction
Network Preview (showing 5 interactants - click image to see 9)
5/9 Interacting proteins for N4BP2 (Q86UW63 ENSP000002614354) via UniProtKB, MINT, STRING, and/or I2D (see all 9)About this table
|
Drugs & Compounds
for N4BP2 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for N4BP2
Search CenterWatch for drugs/clinical trials and news about N4BP2
|
Transcripts
for N4BP2 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for N4BP2 gene: NM_018177.4 Unigene Cluster for N4BP2: NEDD4 binding protein 2 Hs.391463 [show with all ESTs]Unigene Representative Sequence: AB3715844 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000261435(uc003guy.4 uc010ifr.3) ENST00000511480(uc010ifq.3)
ENST00000515550 ENST00000513269
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for N4BP2 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for N4BP2 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 2): N4BP2 (NM_033111) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for N4BP2 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat N4BP2 |
Additional cDNA sequence: AB037834.1 AB371584.1 AK001542.1 AY267013.1 BC126466.1 6 DOTS entries: DT.410570 DT.95070779 DT.99962520 DT.309401 DT.95303939 DT.95303940 24/80 AceView cDNA sequences (see all 80): BM804242 R93882 NM_018177 AK001542 BQ008834 AA598849 BE856543 AI973227 AB037834 H03459 BE550411 BU161412 F05693 AI278279 AU152007 CD652255 AI393829 BM460199 BP349924 AW515216 BF982629 W93336 AI655142 AU129951
GeneLoc Exon Structure
5/6 Alternative Splicing Database (ASD) splice patterns (SP) for N4BP2 (see all 6) About this scheme
| ExUns: | 1 | ^ | 2 | ^ | 3 | ^ | 4 | ^ | 5a | · | 5b | · | 5c | ^ | 6 | ^ | 7 | ^ | 8 | ^ | 9 | ^ | 10 | ^ | 11a | · | 11b | ^ | 12 | ^ | 13 | ^ | 14a | · | 14b | ^ | 15 | ^ | 16 | ^ | 17a | · | 17b | ^ | 18 | ^ | 19 | ^ | 20 |
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| SP1: | | | | | - | | - | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP2: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP3: | | | - | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP5: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
ECgene alternative splicing isoforms for N4BP2
|
Expression
for N4BP2 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| N4BP2 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GTGAATCCCC
About this image
See N4BP2 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for N4BP2
SOURCE GeneReport for Unigene cluster: Hs.391463
SABiosciences Custom PCR Arrays for N4BP2
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for N4BP2
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat N4BP2 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat N4BP2 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat N4BP2 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for N4BP2 |
Orthologs
for N4BP2 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for N4BP2 gene from 6/18 species (see all 18) About this table
ENSEMBL Gene Tree for N4BP2 (if available)
TreeFam Gene Tree for N4BP2 (if available) |
Paralogs
for N4BP2 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for N4BP2 gene
- N4BP2L12
1 SIMAP similar gene for N4BP2 using alignment to 6 protein entries: N4BP2_HUMAN (see all proteins):N4BP2L1
N4BP2 for paralogs About GeneDecksing
2 Pseudogenes.org Pseudogenes for N4BP2
PGOHUM00000246720 PGOHUM00000247046
|
Genomic Variants
for N4BP2 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for N4BP2
1 CNV: 63808 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing N4BP2 |
|
Disorders
/ Diseases
for N4BP2 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
N4BP2 for disorders About GeneDecksing
5  diseases for N4BP2: About MalaCardsleukemia/lymphoma t-cell leukemia leukemia nasopharyngitis
carcinoma
Export disorders for N4BP2 gene to outside databases
|
Publications
for N4BP2 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for N4BP2 gene, integrated from 9 sources (see all 20):
(articles sorted by number of sources associating them with N4BP2) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 10718198)1, 2, 3 Nagase T.... Ohara O. (2000)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- Identification and characterization of BCL-3-binding protein: implications for transcription and DNA repair or recombination. (PubMed id 12730195)1, 2 Watanabe N.... Fujita T. (2003)
- Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase. (PubMed id 11717310)1, 3 Murillas R.... Kuehn M.R. (2002)
- Haplotype of gene Nedd4 binding protein 2 associated with sporadic nasopharyngeal carcinoma in the Southern Chinese population. (PubMed id 17626640)1, 9 Zheng M.Z....Zeng Y.X. (2007)
- Modulated nicking endonuclease function by the N-termi nal extended region of the smr domain in human Bcl-3 binding protein. (PubMed id 22072629)1 Jeong E.Y....Ban C. (2012)
- Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (2012)
- A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
- Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
- Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
|
External Searches for N4BP2 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing N4BP2 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing N4BP2 gene
(According to HUGE)
About This Section
| |
Specialized Databases showing N4BP2 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for N4BP2 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for N4BP2 gene:
Search GeneIP for patents involving N4BP2
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for N4BP2 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat N4BP2 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing N4BP2 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat N4BP2 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat N4BP2 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat N4BP2 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat N4BP2 |
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 | | | N4BP2 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for N4BP2 |
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| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat N4BP2 |
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