N4BP1 Gene
protein-coding GIFtS: 49
GCID: GC16M048572
|
|
NEDD4 binding protein 1
| |
Aliases
for N4BP1 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| NEDD4 Binding Protein 11 2 | | NEDD4-Binding Protein 12 | | KIAA06153 |
Export aliases for N4BP1 gene to outside databasesPrevious GC identifers: GC16M038991 GC16M048624 GC16M048311 GC16M048354 GC16M047131 GC16M034461 |
Summaries
for N4BP1 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: N4BP1_HUMAN, O75113Function: Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH,leading to compete with ITCH's substrates and impairing ubiquitination of substrates (By similarity)
|
Genomic Views
for N4BP1 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000016.9 NC_018927.1 NT_010498.15
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the N4BP1 gene promoter:
HSF1 (long) RelA POU6F1 (c2) NF-kappaB YY1 Max c-Rel Cart-1 HSF1short NF-kappaB1
Other transcription factors
Search SABiosciences Chromatin IP Primers for N4BP1
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat N4BP1 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 16q12.1 Ensembl cytogenetic band: 16q12.1 HGNC cytogenetic band: 16q12.1N4BP1 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 16 GeneLoc Exon Structure GeneLoc location for GC16M048572: view genomic region
(about GC identifiers)
Start:
|
48,572,637 bp from pter |
End:
|
48,654,059 bp from pter |
Size:
|
81,423 bases |
Orientation:
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minus strand |
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Proteins
for N4BP1 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: N4BP1_HUMAN, O75113 (See
protein sequence)Recommended Name: NEDD4-binding protein 1 Size: 896 amino acids; 100379 Da
Subunit: Interacts with NEDD4. Interacts with ITCH (via WW domain 2) (By similarity)
Subcellular location: Nucleus, nucleolus (By similarity). Nucleus, PML body (By similarity). Note=Primarily localizesto the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated (By similarity)
Sequence caution: Sequence=BAA31590.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;
Secondary accessions: A7MD49 Q2YDX1Explore the universe of human proteins at neXtProt for N4BP1: NX_O75113
Post-translational modifications:
Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated with SUMO1,abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PMLnuclear bodies (By similarity)1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_O75113
N4BP1 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_694574.3
ENSEMBL proteins: ENSP00000262384 ENSP00000460667
Human Recombinant Protein Products for N4BP1:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table
N4BP1 for ontologies About GeneDecksing
N4BP1 Antibody Products:
Assay Products for N4BP1: |
Protein
Domains /
Families
for N4BP1 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
N4BP1 for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry O75113ProtoNet protein and cluster: O75113 UniProtKB/Swiss-Prot: N4BP1_HUMAN, O75113Similarity: Belongs to the N4BP1 family |
Function
for N4BP1 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
inGenious Targeting Laboratory,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase,
shRNA from
OriGene,
Sirion Biotech,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Sirion Biotech,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
Sirion Biotech,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Molecular Function:
UniProtKB/Swiss-Prot Summary: N4BP1_HUMAN, O75113Function: Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH,leading to compete with ITCH's substrates and impairing ubiquitination of substrates (By similarity) Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005515 | protein binding |
IPI | -- |
N4BP1 for ontologies About GeneDecksing
Phenotypes:
1 GenomeRNAi human phenotype for N4BP1:
1 MGI mutant phenotype (inferred from 1 allele ) (MGI details for N4bp1):
N4BP1 for phenotypes About GeneDecksing
Animal Models:
Mouse knock-out N4bp1tm1.1Mku for N4BP1
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for N4BP1 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for N4BP1 (see all 2)
OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for N4BP1 |
|
Pathways & Interactions
for N4BP1 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for N4BP1
STRING Interaction
Network Preview (showing 5 interactants - click image to see 6)
5/8 Interacting proteins for N4BP1 (O751131, 2, 3 ENSP000002623844) via UniProtKB, MINT, STRING, and/or I2D (see all 8)About this table
Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0031397 | negative regulation of protein ubiquitination |
ISS | -- | | GO:0032435 | negative regulation of proteasomal ubiquitin-dependent protein catabolic process |
ISS | -- | | GO:0034644 | cellular response to UV |
IMP | 17592138 |
N4BP1 for ontologies About GeneDecksing
|
Drugs & Compounds
for N4BP1 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for N4BP1
Search CenterWatch for drugs/clinical trials and news about N4BP1
|
Transcripts
for N4BP1 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
Sirion Biotech,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for N4BP1 gene: NM_153029.3 Unigene Cluster for N4BP1: NEDD4 binding protein 1 Hs.511839 [show with all ESTs]Unigene Representative Sequence: NM_1530296 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000262384(uc002efp.3) ENST00000565423 ENST00000569027 ENST00000564124
ENST00000565638 ENST00000564710
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for N4BP1 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for N4BP1 (see all 2)
OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: N4BP1 (NM_153029) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for N4BP1 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat N4BP1 |
Additional cDNA sequence: AB014515.1 AK001464.1 AK026937.1 AK056383.1 AK094234.1 AK124782.1 AL831882.1 BC051897.1 BC108288.1 BC152472.1 8 DOTS entries: DT.213542 DT.75103885 DT.112202 DT.209678 DT.86854338 DT.95369347 DT.91713557 DT.40119828 24/179 AceView cDNA sequences (see all 179): AI017927 AA631580 BM968473 AI332705 AI080511 AA911171 BM673246 AA316977 F13173 BM830152 F13171 AI800356 BM707079 BF509350 AL046130 BF512689 BX477474 BQ000756 AB014515 BM677178 F13180 AK001464 AL831882 BM547703
GeneLoc Exon Structure
3 Alternative Splicing Database (ASD) splice patterns (SP) for N4BP1 About this scheme
| ExUns: | 1 | ^ | 2 | ^ | 3a | · | 3b | ^ | 4 | ^ | 5a | · | 5b | ^ | 6 | ^ | 7 | ^ | 8a | · | 8b |
|---|
|
| SP1: | | | - | | | | | | | | | | | | | | | | | | | | |
| SP2: | | | | | | | | | | | | | | | | | - | | | | | | |
| SP3: | | | | | | | | | | | | | | | | | | | | | | |
ECgene alternative splicing isoforms for N4BP1
|
Expression
for N4BP1 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| N4BP1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: ATGTTGTGAC
About this image
See N4BP1 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for N4BP1
SOURCE GeneReport for Unigene cluster: Hs.511839
UniProtKB/Swiss-Prot: N4BP1_HUMAN, O75113Tissue specificity: Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary
SABiosciences Custom PCR Arrays for N4BP1
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for N4BP1
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat N4BP1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat N4BP1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat N4BP1 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for N4BP1 |
Orthologs
for N4BP1 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for N4BP1 gene from 5/18 species (see all 18) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
N4BP11 |
NEDD4 binding protein 1 |
64.48(n)
58.36(a) |
|
415739 NM_001030570.1 NP_001025741.1 |
lizard
(Anolis carolinensis) |
Reptilia |
N4BP16 |
-- |
47(a) |
1 ↔ 1 |
GL343469.1(620140-637372) |
African clawed frog
(Xenopus laevis) |
Amphibia |
n4bp1-pending-prov2 |
Nedd4 binding protein 1 |
71.26(n) |
|
BC045006.1 |
zebrafish
(Danio rerio) |
Actinopterygii |
wufi03b052 |
wufi03b05 |
75.32(n) |
|
327327 BC059205.1 |
fruit fly
(Drosophila melanogaster) |
Insecta |
CG423606 |
-- |
16(a) |
1 → many |
2R(20494812-20498049) |
ENSEMBL Gene Tree for N4BP1 (if available)
TreeFam Gene Tree for N4BP1 (if available) |
Paralogs
for N4BP1 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for N4BP1 gene
- NYNRIN2 ZC3H12C2 ZC3H12B2 ZC3H12D2 KHNYN2 ZC3H12A2
N4BP1 for paralogs About GeneDecksing
|
Genomic Variants
for N4BP1 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 16 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for N4BP1 (48572637 - 48654059 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for N4BP1
1 CNV: 7111 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing N4BP1 |
|
Disorders
/ Diseases
for N4BP1 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
N4BP1 for disorders About GeneDecksing
3  diseases for N4BP1: About MalaCardschronic lymphocytic leukemia lymphocytic leukemia leukemia
Human Genome Epidemiology (HuGE) Navigator: N4BP1 (1 document)
Export disorders for N4BP1 gene to outside databases
|
Publications
for N4BP1 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for N4BP1 gene, integrated from 9 sources (see all 23):
(articles sorted by number of sources associating them with N4BP1) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. (PubMed id 9734811)1, 2, 3 Ishikawa K.... Ohara O. (1998)
- Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase. (PubMed id 11717310)1, 3 Murillas R.... Kuehn M.R. (2002)
- Genome-wide association analysis implicates the involv ement of eight loci with response to tocilizumab for the treatment of rheumatoid arthritis. (PubMed id 22491018)1 Wang J....Platt A. (2012)
- Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (2012)
- A high-throughput approach for measuring temporal chan ges in the interactome. (PubMed id 22863883)1 Kristensen A.R....Foster L.J. (2012)
- Mapping a dynamic innate immunity protein interaction network regulating type I interferon production. (PubMed id 21903422)1 Li S....Dorf M.E. (2011)
- A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
- Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
- Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
- Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. (PubMed id 20068231)2 Olsen J.V....Mann M. (2010)
|
External Searches for N4BP1 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing N4BP1 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing N4BP1 gene
(According to HUGE)
About This Section
| |
Specialized Databases showing N4BP1 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for N4BP1 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for N4BP1 gene:
Search GeneIP for patents involving N4BP1
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for N4BP1 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, LifeMap BioReagents, and Sirion Biotech, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory)
About This Section
|
 | |
 | |
 |
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