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N-PAC Gene

protein-coding   GIFtS: 50

GC16M004793
cytokine-like nuclear factor n-pac
symbol from the EntrezGene Database
NOT approved by the HUGO Gene Nomenclature Committee (HGNC).
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
BM045 2
HIBDL 2, 3
NP60 2
Descriptions
3-hydroxyisobutyrate dehydrogenase-like 2
3-hydroxyisobutyrate dehydrogenase-like protein 3
Nuclear protein of 60 kDa 3
cytokine-like nuclear factor n-pac 2, 3
nuclear protein 60 kDa 2
External Ids
Entrez Gene: 846562
UniProtKB: Q49A263
Ensembl: ENSG000001406327
Search outside databases for aliases for N-PAC gene

Previous GC identifers: GC16M004879 GC16M004853

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: NP60_HUMAN, Q49A26
Function: Regulates p38 MAP kinase activity by mediating stress activation of p38alpha/MAPK14 and
specifically regulating MAPK14 signaling. Indirectly promotes phosphorylation of MAPK14 and
activation of ATF2. The phosphorylation of MAPK14 requires upstream activity of MAP2K4 and MAP2K6

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the N-PAC gene  

Entrez Gene cytogenetic band: 16p13.3   Ensembl cytogenetic band:  16p13.3   

N-PAC Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16M004793:     (about GC identifiers)

Start:
4,793,205 bp from pter
End:
4,837,294 bp from pter
Size:
44,090 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000016.8  NT_037887.4  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: NP60_HUMAN, Q49A26 (See protein sequence)
Recommended Name: Nuclear protein NP60  
Size: 553 amino acids; 60547 Da
Subunit: Interacts with MAPK14
Subcellular location: Nucleus
PDB structures from and Proteopedia :
2UYY (3D)    
Secondary accessions: Q5U632 Q6P1Q2 Q6V3W7 Q9BTI1 Q9BXK2
Alternative splicing: 4 isoforms:  Q49A26-1   Q49A26-2   Q49A26-3   Q49A26-4   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_115958.2  

    ENSEMBL proteins: 
    ENSP00000322716 ENSP00000371413 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (N-PAC)
    Human Recombinant Proteins from Abnova (N-PAC)
                  OriGene Purified Recombinant Human Protein: N-PAC 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    About this table

    Antibodies for N-PAC: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for N-PAC
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (N-PAC), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (N-PAC)
    Novus Biologicals Antibody for N-PAC

    Assays for N-PAC: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/7 InterPro domains/families (see all 7 ):
     IPR006115 6PGDH_NAD-bd
     IPR017956 AT_hook_DNA-bd_CS
     IPR013328 DH_multihelical
     IPR015815 3hydroxyacid_DH/Rdtase
     IPR016040 NAD(P)-bd_dom


       GeneDecks  N-PAC for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q49A26

    ProtoNet protein and cluster: Q49A26

    2 Blocks protein families:
    IPB000313 PWWP domain
    IPB002204 3-hydroxyisobutyrate dehydrogenase


    UniProtKB/Swiss-Prot: NP60_HUMAN, Q49A26
    Domain: The A.T hook DNA-binding domain is required for the interaction with MAPK14
    Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family. NP60 subfamily
    Similarity: Contains 1 A.T hook DNA-binding domain
    Similarity: Contains 1 PWWP domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (N-PAC)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_032569

                  Applied Biosystems Silencer® siRNAs for N-PAC

                  Sigma-Aldrich siRNA for N-PAC  
                         Sigma-Aldrich shRNA for N-PAC  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for N-PAC
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_032569
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_032569
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_032569 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_032569

    UniProtKB/Swiss-Prot: NP60_HUMAN, Q49A26
    Function: Regulates p38 MAP kinase activity by mediating stress activation of p38alpha/MAPK14 and
    specifically regulating MAPK14 signaling. Indirectly promotes phosphorylation of MAPK14 and
    activation of ATF2. The phosphorylation of MAPK14 requires upstream activity of MAP2K4 and MAP2K6

    5 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677 DNA binding IEA--
    GO:0004616 phosphogluconate dehydrogenase (decarboxylating) activity IEA--
    GO:0005488 binding IEA--
    GO:0016491 oxidoreductase activity IEA--
    GO:0050662 coenzyme binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    4 Interacting proteins for N-PAC (ENSP000003227163 Q49A262) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    MAPK14ENSP000002297943STRING (score=.949)
    MAP2K6ENSP000003519973STRING (score=.838)
    MAP2K4ENSP000002624453STRING (score=.714)
    --Q9WMX22MINT-7047931
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006098 pentose-phosphate shunt IEA--
    GO:0008152 metabolic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for N-PAC

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (N-PAC)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_032569

                  Sigma-Aldrich siRNA for N-PAC  
                         Sigma-Aldrich shRNA for N-PAC  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_032569  

    REFSEQ mRNAs for N-PAC gene: 

    NM_032569.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_032569  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_032569
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_032569
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_032569 

    Additional cDNA sequence: 

    AF217521.1 AF244907.1 AF326966.1 AK096730.1 AK296842.1 AK307834.1 AY352585.1 BC003693.1 
    BC022809.1 BC032855.1 BC047223.1 BC048291.1 BC063039.1 BC064940.1 BC071698.1 BC073868.1 
    CR623197.1 CR933687.1 

    19 DOTS entries:

    DT.97820255  DT.455865  DT.97781123  DT.120704602  DT.40107533  DT.97854573  DT.97854571  DT.99936178 
    DT.95335108  DT.92424029  DT.91741097  DT.91881931  DT.100798579  DT.100798583  DT.91875664  DT.91884444 
    DT.92424028  DT.95202224  DT.95326206 

    24/521 AceView cDNA sequences (see all 521 ):

    CR603174 AA346657 BQ941437 AI299980 CB139232 BM703709 CF127496 AI276588 
    BM931710 AI336225 CK725160 AI401496 BE250606 AW272381 CB529066 BU158924 
    BM728680 BM503391 CF135011 CR618679 BE503523 BU855659 CR616895 AW292633 

    highest scoring ESTs for N-PAC:

    AF244907 AF326966 AI671768 AL702600 AY352585 BC032855 BC064940 BI767890 BM808810 BM808969 

    Unigene Cluster for N-PAC:

    Cytokine-like nuclear factor n-pac
    Hs.387255  [show with all ESTs]
    Unigene Representative Sequence: NM_032569


    GeneLoc Exon Structure

    5/8 Alternative Splicing Database (ASD) splice patterns (SP) for N-PAC (see all 8 )

    ExUns: 1 ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c ^ 6a · 6b ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^ 14 ^ 15 ^ 16 ^ 17a · 17b ^ 18 ^ 19
    SP1:              -     -     -                 -                                                                                                               
    SP2:              -     -     -                 -                                                     -                                                         
    SP3:              -     -     -                 -                                   -                                                                           
    SP4:                                            -                                                                                                               
    SP5:              -           -                 -                                                                                                               

    About this scheme

    2 Ensembl transcripts including schematic representations:
    ENST00000321919  ENST00000381983  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    N-PAC expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for N-PAC

    1 / 2 / 3

    14 probe-sets matching N-PAC gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    61265_at2, 3 U95-C 1 1.00 1.00 0.73 0.92 R19745 0.80 1.00 0.91 1

    43465_s_at2, 3 U95-B 1 1.00 1.00 0.70 1.16 AI671768 0.80 1.00 0.91 1

    65970_at2, 3 U95-C 2 0.94 0.66 0.72 1.28 AI560741 0.20 0.33 0.27 2
    86980_at2, 3 U95-E 2 0.88 0.89 0.80 0.67 Z25224 0.80 1.00 0.91 1

    222652_s_at2, 3 U133-B 1 1.00 1.00 -- -- AW089880 0.60 1.00 0.82 1

    218506_x_at2, 3 U133-A 2 1.00 0.91 -- -- NM_018459 0.20 1.00 0.72 1

    221628_s_at2, 3 U133-A 2 1.00 0.86 -- -- AF326966 0.80 1.00 0.91 1

    222115_x_at2, 3 U133-A 2 1.00 0.86 -- -- BC003693 0.60 1.00 0.82 1

    212414_s_at2 U133-A 2 1.00 0.50 -- -- -- -- -- -- --

    222652_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    218506_x_at2 U133Plus2 2 1.00 0.91 -- -- -- -- -- -- --

    221628_s_at2 U133Plus2 2 1.00 0.86 -- -- -- -- -- -- --

    222115_x_at2 U133Plus2 2 1.00 0.86 -- -- -- -- -- -- --

    212414_s_at2 U133Plus2 2 1.00 0.50 -- -- -- -- -- -- --
    GeneDecks  N-PAC for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: ATTTTGTGCA

    SOURCE GeneReport for Unigene cluster: Hs.387255
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for N-PAC gene from 5/12 species (see all 12 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    LOC4798601   -- similar to cytokine-like nuclear factor n-pac 93.97(n)
    99.46(a)
    479860  XM_846566.1  XP_851659.1 
    chimpanzee
    (Pan troglodytes)
    LOC7483281   -- similar to MGC107852 protein 99.94(n)
    100(a)
    748328  XM_001169731.1  XP_001169731.1 
    cow
    (Bos taurus)
    N-PAC1   -- cytokine-like nuclear factor n-pac 91.82(n)
    96.65(a)
    539636  NM_001040568.1  NP_001035658.1 
    rat
    (Rattus norvegicus)
    N-pac1   -- cytokine-like nuclear factor n-pac 92.33(n)
    98.91(a)
    360477  NM_001007800.1  NP_001007801.1 
    mouse
    (Mus musculus)
    3930401K13Rik1, 5 16 (3.40 cM)5
    RIKEN cDNA 3930401K13 gene1, 5 92.81(n)1
    98.73(a)1
    740221  NM_001079814.11  NP_001073282.11 
     AA0087095  AA9395325  (see all 54)
    About this table        Species with no ortholog for N-PAC

    ENSEMBL Gene Tree for N-PAC
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for N-PAC gene
    HIBADH2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/201 NCBI SNPs in N-PAC are shown (see all 201 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 95)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 16 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs20853291,2
    A,C4801710(+) GTCACC/GTGGGC 1 Q/H mis10--------
    --
    rs177031111,2
    C,H4795308(+) TTTTGT/CACACC 1 C/Y mis1 ese37Minor allele frequency- C:0.00NA EU EA WA 542
    rs341762491,2
    C,F4822211(-) CCCACA/GATTCT 1 N/D mis15Minor allele frequency- G:0.00NA EU EA WA 484
    rs80640241,2
    A,C,F,H4795280(+) TTGTCG/AGACTG 1 S/S syn116Minor allele frequency- A:0.46MN EU EA WA NA 1584
    rs118656621,2
    F,H4804548(+) TTGGCC/TGCCTT 1 A/A syn1 ese34Minor allele frequency- T:0.01EU EA WA 418
    rs9308551,2
    A,C,F,H,O4804180(+) CTACCA/GCACTT 1 -- int113Minor allele frequency- G:0.47MN EA NA EU WA 1238
    rs20754671,2
    A,C,F,H,O4813243(+) GTACTC/TATCAG 1 -- int111Minor allele frequency- T:0.38NA EU EA WA 942
    rs65006361,2
    A,C,F,H4815234(+) tctacA/Ggggag 1 -- int14Minor allele frequency- G:0.06EU EA WA 408
    rs99259441,2
    A,C,F,H4825230(+) TCACAG/AGAACC 1 -- int17Minor allele frequency- A:0.48EU EA WA NA 550
    rs133389321,2
    A,C,F,H4818890(+) AAGGTA/GTTTCT 1 -- int111Minor allele frequency- G:0.12EU EA WA NA 980
    About this table

    HapMap Linkage Disequilibrium images for N-PAC (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 610660

    (Possibly Related Articles in Doctor's Guide)
    About This Section

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    6 PubMed articles for N-PAC gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 84656 AceView: FLJ22386andN-PAC Ensembl:ENSG00000140632 euGenes: HUgn84656
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for N-PAC:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for N-PAC-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for N-PAC Antibodies for N-PAC
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for N-PAC Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for N-PAC




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for N-PAC

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