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Magmas Gene

protein-coding GIFtS: 40

GC16M004331

mitochondria-associated protein involved in granulocyte-macrophage colony-stimulating factor signal transduction
symbol from the EntrezGene Database
NOT approved by the HUGO Gene Nomenclature Committee (HGNC).

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
CGI-136 ^²
MAGMAS ^³
TIM16 ^³

Descriptions
Mitochondria-associated granulocyte macrophage CSF-signaling molecule ^³
magmas-like protein ^²
mitochondria-associated granulocyte macrophage CSF signaling molecule ^²
mitochondria-associated protein involved in granulocyte-macrophage colony-stimulating factor signal transduction ^²

External Ids
Entrez Gene: 51025²
UniProtKB: Q9Y3D7³
Ensembl: ENSG00000217930⁷

Search outside databases for aliases for Magmas gene

Previous GC identifers: GC16M004413 GC16M004390

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: TIM16_HUMAN, Q9Y3D7

Function: Probable component of the PAM complex, a complex required for the translocation of

transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an

ATP-dependent manner. May be required to regulate the ATP-dependent activity (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the Magmas gene

Entrez Gene cytogenetic band: 16p13.3 Ensembl cytogenetic band: 16p13.3

Magmas Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 16 GeneLoc Exon Structure

GeneLoc location for GC16M004331: (about GC identifiers)

Start:	4,330,253 bp from pter
End:	4,341,301 bp from pter
Size:	11,049 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000016.8 NT_037887.4

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: TIM16_HUMAN, Q9Y3D7 (See protein sequence)

Recommended Name: Mitochondrial import inner membrane translocase subunit Tim16

Size: 125 amino acids; 13825 Da

Subunit: Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein,

GRPEL1 or GRPEL2, TIMM44, TIMM16/MAGMAS and TIMM14/DNAJC19 (By similarity)

Subcellular location: Mitochondrion inner membrane; Peripheral membrane protein (By similarity)

Secondary accessions: Q6I9Z3 Q9H5X3

REFSEQ proteins: NP_057153.8

ENSEMBL proteins:

ENSP00000315693

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse Abcam for Recombinant Proteins
	Browse Abnova for Human Recombinant Proteins

OriGene Purified Recombinant Human Protein: Magmas

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005739	mitochondrion	IEA	--
GO:0005743	mitochondrial inner membrane	IEA	--
GO:0016020	membrane	IEA	--

About this table

Antibodies for Magmas:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for Magmas

Assays for Magmas:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q9Y3D7

ProtoNet protein and cluster: Q9Y3D7

1 Blocks protein family: IPB005341 Protein of unknown function UPF0108

UniProtKB/Swiss-Prot: TIM16_HUMAN, Q9Y3D7

Domain: The J-like region, although related to the J domain does not have co-chaperone activity (By

similarity)

Similarity: Belongs to the TIM16/PAM16 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (Magmas)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_016069

Applied Biosystems Silencer® siRNAs for Magmas

Sigma-Aldrich siRNA for Magmas

Sigma-Aldrich shRNA for Magmas

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for Magmas
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_016069
Myc/DDK tagged cDNA clone in CMV expression vector: NM_016069
untagged cDNA clone in CMV expression vector: NM_016069

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_016069

UniProtKB/Swiss-Prot: TIM16_HUMAN, Q9Y3D7

Function: Probable component of the PAM complex, a complex required for the translocation of

transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an

ATP-dependent manner. May be required to regulate the ATP-dependent activity (By similarity)

Induction: By GM-CSF

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

2 Interacting proteins for Magmas (Q9Y3D7² ENSP00000315693³) via UniProtKB, MINT, and/or STRING

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
FEZ1	Q99689²	MINT-65847
CBARA1	ENSP00000354415³	STRING (score=.712)

About this table

2 Gene Ontology (GO) biological process terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0015031	protein transport	IEA	--
GO:0065002	intracellular protein transmembrane transport	IEA	--

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for Magmas

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (Magmas)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_016069

Sigma-Aldrich siRNA for Magmas

Sigma-Aldrich shRNA for Magmas

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_016069

REFSEQ mRNAs for Magmas gene:

NM_016069.8

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_016069

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_016069
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_016069
                                 untagged cDNA clone in CMV expression vector: NM_016069

Additional cDNA sequence:

AF151894.1 AF349455.1 AK026514.1 BC005024.1 CR457362.1

18 DOTS entries:

DT.449076 DT.100035684 DT.100035683 DT.40192918 DT.97843012 DT.95162083 DT.100035681 DT.120686847

DT.120686778 DT.120686850 DT.40191766 DT.102831733 DT.91786594 DT.95112830 DT.95162076 DT.95162084

DT.99986281 DT.100842658

24/277 AceView cDNA sequences (see all 277 ):

CA314889 AW249037 BQ882990 NM_016069 AI498278 AI424481 AI057511 AA338859

AW071929 AK026514 AA513366 AI309908 AI922770 AK025674 BX095660 AI801913

AI865884 AI268592 AY313265 AI420340 CB152664 BQ011933 BQ060714 BC005024

highest scoring ESTs for Magmas:

AA338859 AF151894 AF349455 AI089622 AK026514 AU099760 AV706787 AW952786 BC005024 BE907476

Unigene Cluster for Magmas:

Mitochondria-associated protein involved in granulocyte-macrophage colony-stimulating factor signal transduction

Hs.644614 [show with all ESTs]

Unigene Representative Sequence: BQ218379

GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:

ENST00000318059

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

Magmas expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for Magmas

¹ / ² / ³

3 probe-sets matching Magmas gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
44715_at²^{, 3}	U95-B	1	0.94	1.00	1.00	1.00	AI089622	0.80	0.88	0.84	1
218969_at²^{, 3}	U133-A	1	1.00	1.00	--	--	NM_016069	0.60	0.83	0.73	1
218969_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: GCCAAGTACC

SOURCE GeneReport for Unigene cluster: Hs.644614
UniProtKB/Swiss-Prot: TIM16_HUMAN, Q9Y3D7
Tissue specificity: Ubiquitously expressed

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for Magmas gene from 5/14 species (see all 14 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	MAGMAS¹	--	mitochondria-associated protein involved in granulocyte-macrophage more	89.33(n) 94.4(a)	444856 NM_001003391.2 NP_001003391.1
cow (Bos taurus)	Magmas¹	--	mitochondria-associated protein involved in granulocyte-macrophage more	89.6(n) 92(a)	444880 NM_001002893.2 NP_001002893.1
rat (Rattus norvegicus)	Magmas¹	--	mitochondria-associated protein involved in granulocyte-macrophage more	88.44(n) 95.16(a)	679907 XM_001054920.1 XP_001054920.1
mouse (Mus musculus)	ENSMUSG00000045886¹	--	predicted gene, ENSMUSG00000045886	88.8(n) 96(a)	100042179 XM_001477683.1 XP_001477733.1
chicken (Gallus gallus)	MAGMAS¹	--	mitochondria-associated protein involved in granulocyte-macrophage more	76.53(n) 79.2(a)	416669 NM_001004377.2 NP_001004377.1

About this table Species with no ortholog for Magmas

ENSEMBL Gene Tree for Magmas

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/64 NCBI SNPs in Magmas are shown (see all 64 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 38)

AB	SNP ID	Valid	Chr 16 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs11989^1,2	C,F,H	4330359(+)	`CTCCTG/TGGCCT`	1	Q/K	mis¹		11		EA MN EU WA NA	2564
--	rs7193204^1,2	A,C	4343142(+)	`ACACAC/TGTGGT`	1	--	ng3¹		0	--	--	--	--
	rs9939925^1,2	H	4341625(+)	`ATGAAA/GTCAAT`	1	--	ng3¹		4		EU EA WA	370
--	rs12600139^1,2	C	4342159(+)	`gtgaaA/Gcccca`	1	--	ng3¹		0	--	--	--	--
	rs9937854^1,2	A	4341489(+)	`AGGGCA/GAGGGG`	1	--	ng3¹		0	--	--	--	--
	rs2072151^1,2	A,C,F,H	4331041(-)	`TGCCCA/GACATC`	1	--	int¹		5		EA EU WA	1908
--	rs12445693^1,2	A,C,F,H	4331734(+)	`GCTAAT/ACCTGG`	1	--	int¹		3		EU EA	300
	rs611704^1,2	C,F,H	4335242(-)	`TTGACC/TGAAGG`	1	--	int¹		8		EA NA MN EU WA	664
	rs8057968^1,2	C,F,H	4335976(+)	`TTGCAA/GTCACT`	1	--	int¹		4		EU EA WA	416
--	rs495664^1,2	A,C	4340284(+)	`gctaaC/Tatatg`	1	--	int¹		0	--	--	--	--

About this table

HapMap Linkage Disequilibrium images for Magmas (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

8 PubMed articles for Magmas gene:

Identification and characterization of Magmas, a novel mitochondria- associated protein involved in granulocyte-macrophage colony- stimulating factor signal transduction. (PubMed id 11750097)^{1, 3, 4} Jubinsky P.T.... Short M.K. (2001)
Magmas expression in neoplastic human prostate. (PubMed id 15704001)^{3, 4} Jubinsky P.T....Li M. (2005)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{3, 4} Ota T....Sugano S. (2004)
Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics. (PubMed id 10810093)^{3, 4} Lai C.H....Lin W. (2000)
A human protein-protein interaction network: a resource for annotating the proteome. (PubMed id 16169070)³ Stelzl U....Wanker E.E. (2005)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
Structure and function of Tim14 and Tim16, the J and J-like components of the mitochondrial protein import motor. (PubMed id 16977310)¹ Mokranjac D....Groll M. (2006)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 51025

AceView: MagmasandCRN7

Ensembl:ENSG00000217930

euGenes: HUgn51025

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for Magmas
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for Magmas:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for Magmas-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for Magmas		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for Magmas		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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	Tagged/untagged cDNA clones
	Validated SYBR primer pairs
	Purified proteins from HEK293T cells
	Western blot validated antibodies
	shRNA in GFP-retroviral vector

Search Tocris compounds for Magmas

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Search Novus for antibodies for Magmas

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Magmas Gene

protein-coding GIFtS: 40

Aliases &
Descriptions
for Magmas

Summaries
for Magmas

Genomic Location
for Magmas

Proteins
for Magmas

Protein Domains/
Families
for Magmas

Gene Function
for Magmas

Pathways & Interactions
for Magmas

Drugs & Compounds
for Magmas

Transcripts
for Magmas

Expression
for Magmas

Orthologs
for Magmas

Paralogs
for Magmas

SNPs/Variants
for Magmas

Disorders & Mutations
for Magmas

Medical News
for Magmas

Publications
for Magmas

Search for Magmas

Genome Databases showing Magmas

Other Databases showing Magmas

Specialized Databases showing Magmas

Licensable Technologies
for Magmas

Services
for Magmas

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Magmas Gene

protein-coding GIFtS: 40

Aliases & Descriptionsfor Magmas

Summariesfor Magmas

Genomic Locationfor Magmas

Proteinsfor Magmas

Protein Domains/Familiesfor Magmas

Gene Functionfor Magmas

Pathways & Interactionsfor Magmas

Drugs & Compoundsfor Magmas

Transcriptsfor Magmas

Expression for Magmas

Orthologsfor Magmas

Paralogsfor Magmas

SNPs/Variantsfor Magmas

Disorders & Mutationsfor Magmas

Medical Newsfor Magmas

Publicationsfor Magmas

Search for Magmas

Genome Databases showing Magmas

Other Databases showing Magmas

Specialized Databases showing Magmas

Licensable Technologiesfor Magmas

Servicesfor Magmas

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for Magmas

Summaries
for Magmas

Genomic Location
for Magmas

Proteins
for Magmas

Protein Domains/
Families
for Magmas

Gene Function
for Magmas

Pathways & Interactions
for Magmas

Drugs & Compounds
for Magmas

Transcripts
for Magmas

Expression
for Magmas

Orthologs
for Magmas

Paralogs
for Magmas

SNPs/Variants
for Magmas

Disorders & Mutations
for Magmas

Medical News
for Magmas

Publications
for Magmas

Licensable Technologies
for Magmas

Services
for Magmas