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MZT2B Gene

protein-coding   GIFtS: 48
GCID: GC02P130941

Mitotic Spindle Organizing Protein 2B

(Previous names: family with sequence similarity 128, member B)
(Previous symbol: FAM128B)
  Search for MZT2B
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Mitotic Spindle Organizing Protein 2B1 2
FAM128B1 2 3 5
Mitotic-Spindle Organizing Protein Associated With A Ring Of Gamma-Tubulin
2B1 2 3
MOZART2B2 3 5
Family With Sequence Similarity 128, Member B1 2
Mitotic-Spindle Organizing Protein 2B2

External Ids:    HGNC: 258861   Entrez Gene: 800972   Ensembl: ENSG000001520827   OMIM: 6134505   UniProtKB: Q6NZ673   

Export aliases for MZT2B gene to outside databases

Previous GC identifer: GC02P123235


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for MZT2B Gene:
MZT2B (mitotic spindle organizing protein 2B) is a protein-coding gene. An important paralog of this gene is MZT2A.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000002.11  NT_005403.18  NC_018913.2  
Regulatory elements:
   Search for regulatory transcription factor binding sites for MZT2B
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMZT2B promoter sequence
   Search Chromatin IP Primers for MZT2B

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MZT2B


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2q21.1   Ensembl cytogenetic band:  2q21.1   HGNC cytogenetic band: 2q21.1

MZT2B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MZT2B gene location

GeneLoc information about chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P130941:  view genomic region     (about GC identifiers)

Start:
130,939,310 bp from pter      End:
130,948,302 bp from pter
Size:
8,993 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: MZT2B_HUMAN, Q6NZ67 (See protein sequence)
Recommended Name: Mitotic-spindle organizing protein 2B  
Size: 158 amino acids; 16226 Da
Subunit: Part of the gamma-tubulin complex. Interacts with TUBG1
Secondary accessions: Q96CG4

Explore the universe of human proteins at neXtProt for MZT2B: NX_Q6NZ67

Explore proteomics data for MZT2B at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See MZT2B Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_079305.2  
    ENSEMBL proteins: 
     ENSP00000398749   ENSP00000387805   ENSP00000281871   ENSP00000386419   ENSP00000404629  

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    Cloud-Clone Corp. Proteins for MZT2B

     
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    Search eBioscience for ELISAs for MZT2B 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    1 InterPro protein domain:
     IPR024332 MOZART2

    Graphical View of Domain Structure for InterPro Entry Q6NZ67

    ProtoNet protein and cluster: Q6NZ67

    UniProtKB/Swiss-Prot: MZT2B_HUMAN, Q6NZ67
    Similarity: Belongs to the MOZART2 family


    Find genes that share domains with MZT2B           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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         Gene Ontology (GO): 1 molecular function term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI--
         
    Find genes that share ontologies with MZT2B           About GenesLikeMe


    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MZT2B
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    miRNA
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    miRTarBase miRNAs that target MZT2B:
    hsa-mir-186-5p (MIRT045174), hsa-mir-484 (MIRT042318)

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    Predesigned siRNA for gene silencing in human, mouse, rat MZT2B

    Gene Editing
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    Clone
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    GenScript: all cDNA clones in your preferred vector: MZT2B (NM_025029)
    Browse Sino Biological Human cDNA Clones
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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    MZT2B_HUMAN, Q6NZ67: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton,
    spindle
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytoskeleton5
    nucleus3
    cytosol2
    mitochondrion2

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005813centrosome IDA--
    GO:0005819spindle IDA--
    GO:0008274gamma-tubulin ring complex IDA--

    Find genes that share ontologies with MZT2B           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for MZT2B
    Interactions:

        Search GeneGlobe Interaction Network for MZT2B

    STRING Interaction Network Preview (showing 5 interactants - click image to see 14)

    Selected Interacting proteins for MZT2B (Q6NZ671, 2, 3 ENSP000002818714) via UniProtKB, MINT, STRING, and/or I2D (see all 17)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SETDB1Q150472, 3, ENSP000003579654MINT-8270264 I2D: score=2 STRING: ENSP00000357965
    CDC37Q165432, 3MINT-8250866 I2D: score=2 
    TUBG1P232581, 3, ENSP000002514134EBI-1052566,EBI-302589 I2D: score=1 STRING: ENSP00000251413
    MYCP011063, ENSP000003672074I2D: score=1 STRING: ENSP00000367207
    TUBGCP2Q9BSJ23, ENSP000002529364I2D: score=1 STRING: ENSP00000252936
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for MZT2B



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for MZT2B gene: 
    NM_025029.3  

    Unigene Cluster for MZT2B:

    Mitotic spindle organizing protein 2B
    Hs.469925  [show with all ESTs]
    Unigene Representative Sequence: BI758897
    8 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000425361 ENST00000457492 ENST00000281871(uc002tqu.3) ENST00000409255
    ENST00000455239 ENST00000485869 ENST00000480182 ENST00000491178
    miRNA
    Products:
         
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    Browse SwitchGear 3'UTR luciferase reporter plasmids
    Inhib. RNA
    Products:
         
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    Predesigned siRNA for gene silencing in human, mouse, rat MZT2B
    Clone
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    GenScript: all cDNA clones in your preferred vector: MZT2B (NM_025029)
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    Primer
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    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MZT2B
      QuantiTect SYBR Green Assays in human, mouse, rat MZT2B
      QuantiFast Probe-based Assays in human, mouse, rat MZT2B

    Additional mRNA sequence: 

    BC014255.2 BC066296.1 

    Selected DOTS entries (see all 25):

    DT.95357791  DT.100040544  DT.95288728  DT.95287225  DT.40285940  DT.92470739  DT.91882168  DT.40234559 
    DT.120981153  DT.100828524  DT.120980873  DT.120980939  DT.95357792  DT.100040442  DT.100691549  DT.435893 
    DT.91644653  DT.92470725  DT.95148741  DT.95148742  DT.95203704  DT.95253479  DT.100687923  DT.100800109 

    Selected AceView cDNA sequences (see all 210):

    BQ893573 AI138643 BI792804 BQ057792 AI474470 BI793053 BC014255 AI494321 
    BE875101 CR624733 AI498751 AL046700 CR623609 AJ572218 BQ687799 AI218442 
    BQ879474 CD673427 F32252 BM785240 AI658859 NM_025029 CK820989 BU607442 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    MZT2B expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: --
    MZT2B Expression
    About this image


    MZT2B expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 13) fully expand
     
     Thymus (Hematopoietic System)
             Double Positive Thymocytes Thymus
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Blood (Cardiovascular System)
             Double Positive Thymocytes Thymus
     
     Colon (Gastrointestinal Tract)
     
     Kidney (Urinary System)
    MZT2B Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    MZT2B Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.469925
        Custom PCR Arrays for MZT2B
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MZT2B

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of chordates.

    Orthologs for MZT2B gene from Selected species (see all 9)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Mzt25 mitotic spindle organizing protein 2   --   16 (10.09 cM) 15848441 
    chicken
    (Gallus gallus)
    Aves --
    Uncharacterized protein
    54(a)
    1 → many
    10(342472-350845) ENSGALG00000002541
    lizard
    (Anolis carolinensis)
    Reptilia --
    --
    Uncharacterized protein
    54(a)
    41(a)
    many ↔ many
    many ↔ many
    GL343782.1(77329-90479)
    GL343669.1(98159-98380)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia mzt2b1 mitotic spindle organizing protein 2B 60.43(n)
    56.13(a)
      733867  NM_001045607.1  NP_001039072.1 
    zebrafish
    (Danio rerio)
    Actinopterygii mzt2b1 mitotic spindle organizing protein 2B 59.06(n)
    57.89(a)
      445174  NM_001003568.2  NP_001003568.1 


    ENSEMBL Gene Tree for MZT2B (if available)
    TreeFam Gene Tree for MZT2B (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for MZT2B gene
    MZT2A2  
    1 SIMAP similar gene for MZT2B using alignment to 5 protein entries:     MZT2B_HUMAN (see all proteins):
    MZT2A

    Find genes that share paralogs with MZT2B           About GenesLikeMe


    2 Pseudogenes.org Pseudogenes for MZT2B
    PGOHUM00000248508 PGOHUM00000246254


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for MZT2B (see all 383)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 2 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1910808571,2
    --123233274(+) GATCCA/GCTGCC 1 -- us2k10--------
    rs1807522201,2
    --123233368(+) ATCTCA/GGCTCA 1 -- us2k10--------
    rs1431055821,2
    C--123233454(+) CCACCA/CCATCC 1 -- us2k10--------
    rs1807596301,2
    --123239489(+) CAGACA/GAGGAA 1 -- int10--------
    rs2021699651,2
    --123239573(+) GTGAC-/ATGTGAT 1 -- int10--------
    rs1398963821,2
    --123239793(+) TCAAGA/GGTCAT 1 -- int10--------
    rs1864482081,2
    --123239915(+) GAACTC/GGGTAA 1 -- int10--------
    rs1492976871,2
    --123239916(+) AACTCA/GGTAAT 1 -- int10--------
    rs1910007881,2
    --123239918(+) CTCGGG/TAATAA 1 -- int10--------
    rs2005466601,2
    --123239926(+) TAACA-/TGACCT 1 -- int10--------

    HapMap Linkage Disequilibrium report for MZT2B (130939310 - 130948302 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for MZT2B (see all 25):    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv834375CNV Loss17160897
    nsv459274CNV Loss19166990
    nsv428403CNV Loss18775914
    nsv821629CNV Loss15273396
    dgv4306n71CNV Gain21882294
    dgv475n27CNV Gain19166990
    nsv875061CNV Gain21882294
    nsv482053CNV Gain20164927
    nsv818080CNV Gain17921354
    nsv875087CNV Gain21882294

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing MZT2B
    DNA2.0 Custom Variant and Variant Library Synthesis for MZT2B

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 613450    OMIM disorders: --

    1 disease from the University of Copenhagen DISEASES database for MZT2B:
    Microcephaly

    Find genes that share disorders with MZT2B           About GenesLikeMe

    Genetic Association Database (GAD): MZT2B
    Human Genome Epidemiology (HuGE) Navigator: MZT2B (3 documents)

    Export disorders for MZT2B gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for MZT2B gene, integrated from 10 sources (see all 17):
    (articles sorted by number of sources associating them with MZT2B)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Systematic analysis of human protein complexes identifies chromosome segregation proteins. (PubMed id 20360068)1, 2, 3 Hutchins J.R.... Peters J.M. (Science 2010)
    2. Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. (PubMed id 20628086)1, 4 Bailey S.D....Anand S. (Diabetes Care 2010)
    3. Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS. (PubMed id 23455922)1 Varjosalo M....Superti-Furga G. (Nat. Methods 2013)
    4. Pathway Analysis of ChIP-Seq-Based NRF1 Target Genes Suggests a Logical Hypothesis of their Involvement in the Pathogenesis of Neurodegenerative Diseases. (PubMed id 24250222)1 Satoh J....Yamamoto Y. (Gene Regul Syst Bio 2013)
    5. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (BMC Syst. Biol. 2011)
    6. A directed protein interaction network for investigating intracellular signal transduction. (PubMed id 21900206)1 Vinayagam A....Wanker E.E. (Sci Signal 2011)
    7. Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (amp 2011)
    8. Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. (PubMed id 20068231)2 Olsen J.V....Mann M. (Sci. Signal. 2010)
    9. Proteomic profiling of Myc-associated proteins. (PubMed id 21150319)1 Agrawal P....Sedivy J.M. (Cell Cycle 2010)
    10. CDK5RAP2 stimulates microtubule nucleation by the gamma-tubulin ring complex. (PubMed id 21135143)1 Choi Y.K....Qi R.Z. (J. Cell Biol. 2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 80097 HGNC: 25886 AceView: FLJ14346 Ensembl:ENSG00000152082 euGenes: HUgn80097
    ECgene: MZT2B H-InvDB: MZT2B

    (According to HUGE)
    About This Section

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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for MZT2B Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for MZT2B gene:
    Search GeneIP for patents involving MZT2B

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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