MYLK4 Gene
protein-coding GIFtS: 47
GCID: GC06M002663
|
|
myosin light chain kinase family, member 4
| |
Aliases
for MYLK4 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Myosin Light Chain Kinase Family, Member 41 2 | | CaMLCK Like2 | | SgK0851 2 3 | | Myosin Light Chain Kinase Family Member 42 | | Sugen Kinase 852 3 | | SGK0853 | | EC 2.7.11.13 8 | | EC 2.7.118 |
Export aliases for MYLK4 gene to outside databasesPrevious GC identifer: GC06M002613 |
Summaries
for MYLK4 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
 summary
for MYLK4: Myosin Light Chain Kinases (MLCKs) are a group of protein serine/threonine kinases that is currently divided
into two subtypes, MLCK1 and MLCK2. MLCK1 is found in smooth muscle and phosphorylates myosin II regulatory
light chains at Ser19, allowing myosin crossbridges to bind to actin filaments and initiate contraction. In
addition to smooth muscle contraction, MLCK1 is involved in a diverse range of biological processes
including control of endothelial and vascular permeability, growth initiation of astrocytes processes,
neurotransmitter release in the sympathetic nervous system and apoptosis of fibroblasts. MLCK2 is found in
striated muscle and co-localizes with phosphorylated MLC filaments to mediate skeletal and cardiac muscle
contraction. MLCK3 and MLCK4 have recently been identified but are yet to be fully characterized. MLCKs are
activated by Ca2+-calmodulin complexes and require Mg2+ or Ca2+ as a co-factor.
|
Genomic Views
for MYLK4 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000006.11 NC_018917.1 NT_007592.15
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the MYLK4 gene promoter:
c-Fos AP-1 Fra-1 FosB HNF-4alpha2 HNF-4alpha1 MEF-2A aMEF-2 c-Jun ATF
Other transcription factors
Search SABiosciences Chromatin IP Primers for MYLK4
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MYLK4 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 6p25.2 Ensembl cytogenetic band: 6p25.2 HGNC cytogenetic band: 6p25.2MYLK4 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 6 GeneLoc Exon Structure GeneLoc location for GC06M002663: view genomic region
(about GC identifiers)
Start:
|
2,663,863 bp from pter |
End:
|
2,751,200 bp from pter |
Size:
|
87,338 bases |
Orientation:
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minus strand |
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Proteins
for MYLK4 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: MYLK4_HUMAN, Q86YV6 (See
protein sequence)Recommended Name: Myosin light chain kinase family member 4 Size: 388 amino acids; 44508 Da
1 PDB 3D structure from  and Proteopedia  for MYLK4:2X4F (3D)
Secondary accessions: A2RUC0 Q5TAW2Alternative splicing: 2 isoforms: Q86YV6-1 Q86YV6-2 (Sequence incomplete. No experimental confirmation available)Explore the universe of human proteins at neXtProt for MYLK4: NX_Q86YV6
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q86YV6
4/7 DME Specific Peptides for MYLK4 (Q86YV6) (see all 7)
MYLK4 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_001012418.2
ENSEMBL proteins: ENSP00000274643 ENSP00000268446
Human Recombinant Protein Products:
MYLK4 Antibody Products:
Assay Products for MYLK4: |
Protein
Domains /
Families
for MYLK4 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
MYLK4 for domains About GeneDecksing
5/6 InterPro domains/families (see all 6):Graphical View of Domain Structure for InterPro Entry Q86YV6ProtoNet protein and cluster: Q86YV6 UniProtKB/Swiss-Prot: MYLK4_HUMAN, Q86YV6Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase familySimilarity: Contains 1 protein kinase domain |
Function
for MYLK4 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: MYLK4_HUMAN, Q86YV6Catalytic activity: ATP + a protein = ADP + a phosphoproteinEnzyme Numbers (IUBMB): EC 2.7.11.11 2 EC 2.7.112
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MYLK4 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MYLK4
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MYLK4 |
Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0004672 | protein kinase activity |
-- | -- | | GO:0004674 | protein serine/threonine kinase activity |
IEA | -- | | GO:0005524 | ATP binding |
IEA | -- |
MYLK4 for ontologies About GeneDecksing
3 GenomeRNAi human phenotypes for MYLK4:
|
Pathways & Interactions
for MYLK4 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for MYLK4
|
Drugs & Compounds
for MYLK4 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
Compounds for MYLK4 available from Tocris Bioscience About this table
2 HMDB Compounds for MYLK4 About this table
Search CenterWatch for drugs/clinical trials and news about MYLK4
|
Transcripts
for MYLK4 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for MYLK4 gene: NM_001012418.3 Unigene Cluster for MYLK4: Myosin light chain kinase family, member 4 Hs.127830 [show with all ESTs]Unigene Representative Sequence: BX6475822 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000274643(uc003mty.4) ENST00000268446(uc003mtx.4)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MYLK4 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MYLK4
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: MYLK4 (NM_001012418) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for MYLK4 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MYLK4 |
Additional cDNA sequence: AK090753.1 AK096893.1 AK122581.1 AK127360.1 BC132831.1 BC132833.1 BX647582.1 3 DOTS entries: DT.205328 DT.91806915 DT.75172229 17 AceView cDNA sequences: AI683999 AK096893 AA862971 BU662434 BU675945 AK122581 AK090753 AW005603 BQ029866 BX647582 AK127360 BI899555 AW451640 BF736629 H89078 H88988 C04755
GeneLoc Exon Structure
|
Expression
for MYLK4 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| MYLK4 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
See MYLK4 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for MYLK4
SOURCE GeneReport for Unigene cluster: Hs.127830
SABiosciences Custom PCR Arrays for MYLK4
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for MYLK4
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse / rat MYLK4 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat MYLK4 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MYLK4 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MYLK4 |
Orthologs
for MYLK4 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for MYLK4 gene from 6/21 species (see all 21) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
MYLK41 |
myosin light chain kinase family, member 4 |
76.84(n)
83.46(a) |
|
420893 XM_418978.3 XP_418978.3 |
lizard
(Anolis carolinensis) |
Reptilia |
MYLK46 |
-- |
81(a) |
1 ↔ 1 |
GL343311.1(970854-985209) |
African clawed frog
(Xenopus laevis) |
Amphibia |
Xl.23722 |
Xenopus laevis transcribed sequence with weak similarity more |
70.78(n) |
|
BG407476.1 |
zebrafish
(Danio rerio) |
Actinopterygii |
LOC5668451 |
uncharacterized LOC566845 |
71.61(n)
72.76(a) |
|
566845 XM_690132.5 XP_695224.5 |
fruit fly
(Drosophila melanogaster) |
Insecta |
Unc-896sqa6 |
spaghetti-squash activator |
3(a)16(a) |
possible ortholog1 ↔ many |
2R(19883066-19901384) 2R(5603981-5635623) |
worm
(Caenorhabditis elegans) |
Secernentea |
ZC373.46 |
Protein ZC373.4 |
11(a) |
possible ortholog |
X(10062379-10069725) |
ENSEMBL Gene Tree for MYLK4 (if available)
TreeFam Gene Tree for MYLK4 (if available) |
Paralogs
for MYLK4 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for MYLK4 gene
- STK17B2 DAPK22 MYLK22 TTN2 MYLK32 STK17A2 OBSCN2 DAPK32
- SPEG2 DAPK12 MYLK2 OBSL12
18/47 SIMAP similar genes for MYLK4 using alignment to 2 protein entries: MYLK4_HUMAN (see all proteins)
(see all similar genes):MYLK3 MYLK2 TTN MYLK DAPK2 DAPK1
DAPK3 MARK2 RPS6KA1 STK36 STK17A RPS6KA2
BRSK2 STK17B CAMK4 KALRN CAMK1 CAMK1D
MYLK4 for paralogs About GeneDecksing
|
Genomic Variants
for MYLK4 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 6 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for MYLK4 (2663863 - 2751200 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 10 variations for MYLK4
9 CNVs: 93457 81204 93455 93459 81207 93456 93458 81206 81205
1 Indel: 99404 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing MYLK4 |
|
Disorders
/ Diseases
for MYLK4 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
MYLK4 for disorders About GeneDecksing
1  disease for MYLK4: About MalaCardsalcoholism
Human Genome Epidemiology (HuGE) Navigator: MYLK4 (1 document)
Export disorders for MYLK4 gene to outside databases
|
Publications
for MYLK4 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for MYLK4 gene integrated from 9 sources:
(articles sorted by number of sources associating them with MYLK4) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- The DNA sequence and analysis of human chromosome 6. (PubMed id 14574404)1, 2 Mungall A.J.... Beck S. (2003)
- The protein kinase complement of the human genome. (PubMed id 12471243)1, 2 Manning G.... Sudarsanam S. (2002)
- Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1 Rose J.E....Uhl G.R. (2010)
- Dynamics of cullin-RING ubiquitin ligase network revea led by systematic quantitative proteomics. (PubMed id 21145461)1 Bennett E.J....Harper J.W. (2010)
- Patterns of somatic mutation in human cancer genomes. (PubMed id 17344846)2 Greenman C.... Stratton M.R. (2007)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1 Ota T.... Sugano S. (2004)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
|
External Searches for MYLK4 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing MYLK4 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing MYLK4 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing MYLK4 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for MYLK4 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for MYLK4 gene:
Search GeneIP for patents involving MYLK4
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for MYLK4 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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| |
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| | | | Tocris compounds for MYLK4 |
| |  |  |  |  | | | | |
 |
 |
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 | | | MYLK4 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MYLK4 |
|  |  |  | | | | ThermoFisher Antibodies for MYLK4 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MYLK4 |
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