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Aliases for MYL12B Gene

Aliases for MYL12B Gene

  • Myosin, Light Chain 12B, Regulatory 2 3
  • MRLC2 3 4 6
  • Myosin Regulatory Light Chain 2-B, Smooth Muscle Isoform 3 4
  • Myosin Regulatory Light Chain 20 KDa 3 4
  • Myosin Regulatory Light Chain MRLC2 3 4
  • Myosin Regulatory Light Chain 2 2 3
  • SHUJUN-1 3 4
  • MLC-2a 3 4
  • MLC-2 3 4
  • MLC20 3 4
  • Myosin Regulatory Light Chain 12B 3
  • MYLC2B 4
  • MLC-B 3

External Ids for MYL12B Gene

Previous GeneCards Identifiers for MYL12B Gene

  • GC18P003254

Summaries for MYL12B Gene

Entrez Gene Summary for MYL12B Gene

  • The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM, Mar 2008]

GeneCards Summary for MYL12B Gene

MYL12B (Myosin, Light Chain 12B, Regulatory) is a Protein Coding gene. Among its related pathways are RhoGDI Pathway and Regulation of actin cytoskeleton. GO annotations related to this gene include calcium ion binding. An important paralog of this gene is MYL12A.

UniProtKB/Swiss-Prot for MYL12B Gene

  • Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion (By similarity).

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MYL12B Gene

Genomics for MYL12B Gene

Regulatory Elements for MYL12B Gene

Genomic Location for MYL12B Gene

Start:
3,261,909 bp from pter
End:
3,278,284 bp from pter
Size:
16,376 bases
Orientation:
Plus strand

Genomic View for MYL12B Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for MYL12B Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MYL12B Gene

Proteins for MYL12B Gene

  • Protein details for MYL12B Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O14950-ML12B_HUMAN
    Recommended name:
    Myosin regulatory light chain 12B
    Protein Accession:
    O14950
    Secondary Accessions:
    • D3DUH6
    • Q13182
    • Q7Z5Z4

    Protein attributes for MYL12B Gene

    Size:
    172 amino acids
    Molecular mass:
    19779 Da
    Quaternary structure:
    • Myosin is a hexamer of 2 heavy chains and 4 light chains
    Miscellaneous:
    • This chain binds calcium.
    SequenceCaution:
    • Sequence=AAP73808.1; Type=Frameshift; Positions=4; Evidence={ECO:0000305};

neXtProt entry for MYL12B Gene

Proteomics data for MYL12B Gene at MOPED

Post-translational modifications for MYL12B Gene

  • Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment.
  • Ubiquitination at Lys51, Lys150, and Lys151
  • Modification sites at PhosphoSitePlus

Other Protein References for MYL12B Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

No data available for DME Specific Peptides for MYL12B Gene

Domains for MYL12B Gene

Gene Families for MYL12B Gene

HGNC:
  • EFHAND :EF-hand domain containing
  • MYL :Myosins / Light chain

Protein Domains for MYL12B Gene

Suggested Antigen Peptide Sequences for MYL12B Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

O14950

UniProtKB/Swiss-Prot:

ML12B_HUMAN :
  • O14950
Domain:
  • Contains 3 EF-hand domains.
genes like me logo Genes that share domains with MYL12B: view

Function for MYL12B Gene

Molecular function for MYL12B Gene

UniProtKB/Swiss-Prot Function: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion (By similarity).

Gene Ontology (GO) - Molecular Function for MYL12B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005509 calcium ion binding IEA --
GO:0005515 protein binding IPI 19328794
genes like me logo Genes that share ontologies with MYL12B: view

Phenotypes for MYL12B Gene

genes like me logo Genes that share phenotypes with MYL12B: view

Animal Model Products

CRISPR Products

miRNA for MYL12B Gene

miRTarBase miRNAs that target MYL12B

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targeting and HOMER Transcription for MYL12B Gene

Localization for MYL12B Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for MYL12B Gene COMPARTMENTS Subcellular localization image for MYL12B gene
Compartment Confidence
cytosol 4
cytoskeleton 2
mitochondrion 2
nucleus 2
endoplasmic reticulum 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for MYL12B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001725 stress fiber IEA --
GO:0005829 cytosol TAS --
GO:0016460 myosin II complex IEA --
GO:0030018 Z disc IEA --
GO:0045177 apical part of cell IEA --
genes like me logo Genes that share ontologies with MYL12B: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for MYL12B Gene

Pathways for MYL12B Gene

genes like me logo Genes that share pathways with MYL12B: view

Gene Ontology (GO) - Biological Process for MYL12B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006936 muscle contraction TAS --
GO:0007264 small GTPase mediated signal transduction TAS --
GO:0007411 axon guidance TAS --
GO:0008360 regulation of cell shape IEA --
GO:0048013 ephrin receptor signaling pathway TAS --
genes like me logo Genes that share ontologies with MYL12B: view

Compounds for MYL12B Gene

(1) HMDB Compounds for MYL12B Gene

Compound Synonyms Cas Number PubMed IDs
Calcium
  • Ca
7440-70-2
genes like me logo Genes that share compounds with MYL12B: view

Transcripts for MYL12B Gene

Unigene Clusters for MYL12B Gene

Myosin, light chain 12B, regulatory:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for MYL12B

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for MYL12B Gene

No ASD Table

Relevant External Links for MYL12B Gene

GeneLoc Exon Structure for
MYL12B
ECgene alternative splicing isoforms for
MYL12B

Expression for MYL12B Gene

mRNA expression in normal human tissues for MYL12B Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for MYL12B Gene

SOURCE GeneReport for Unigene cluster for MYL12B Gene Hs.464472

mRNA Expression by UniProt/SwissProt for MYL12B Gene

O14950-ML12B_HUMAN
Tissue specificity: Ubiquitously expressed in various hematopoietic cells.
genes like me logo Genes that share expressions with MYL12B: view

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for MYL12B Gene

Orthologs for MYL12B Gene

This gene was present in the common ancestor of animals.

Orthologs for MYL12B Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MYL12B 35
  • 99.81 (n)
  • 100 (a)
MYL12B 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia MYL12A 35
  • 90.12 (n)
  • 98.84 (a)
MYL12B 36
  • 99 (a)
OneToOne
dog
(Canis familiaris)
Mammalia MYL12A 35
  • 92.25 (n)
  • 98.84 (a)
MYL12B 36
  • 99 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Myl12a 35
  • 86.43 (n)
  • 97.67 (a)
Myl12b 16
Myl12b 36
  • 100 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia MYL12B 36
  • 100 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 94 (a)
OneToMany
rat
(Rattus norvegicus)
Mammalia Myl12a 35
  • 87.02 (n)
  • 97.67 (a)
chicken
(Gallus gallus)
Aves -- 36
  • 99 (a)
ManyToMany
LOC770011 35
  • 86.43 (n)
  • 98.84 (a)
lizard
(Anolis carolinensis)
Reptilia -- 36
  • 97 (a)
ManyToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia myl12b 35
  • 81.98 (n)
  • 94.77 (a)
zebrafish
(Danio rerio)
Actinopterygii myl12.1 36
  • 92 (a)
ManyToMany
myl12.2 35
  • 79.84 (n)
  • 94.77 (a)
myl12.2 36
  • 95 (a)
ManyToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP000927 35
  • 73.63 (n)
  • 82.91 (a)
fruit fly
(Drosophila melanogaster)
Insecta sqh 35
  • 75.53 (n)
  • 84.18 (a)
sqh 36
  • 81 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea mlc-4 35
  • 70.46 (n)
  • 75.95 (a)
mlc-4 36
  • 72 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.5139 36
  • 80 (a)
OneToMany
Species with no ortholog for MYL12B:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for MYL12B Gene

ENSEMBL:
Gene Tree for MYL12B (if available)
TreeFam:
Gene Tree for MYL12B (if available)

Paralogs for MYL12B Gene

Paralogs for MYL12B Gene

Selected SIMAP similar genes for MYL12B Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with MYL12B: view

Variants for MYL12B Gene

Sequence variations from dbSNP and Humsavar for MYL12B Gene

SNP ID Clin Chr 18 pos Sequence Context AA Info Type MAF
rs11650 -- 3,262,195(+) GTTTG(C/T)GGTCT upstream-variant-2KB, utr-variant-5-prime
rs949302 -- 3,264,157(+) CATAG(C/G)TAGAC intron-variant
rs949303 -- 3,264,351(+) CTGCC(A/G)TGTGC intron-variant
rs1059935 -- 3,278,183(+) GCCCA(A/G)AAAAA utr-variant-3-prime
rs1372996 -- 3,278,477(-) ACATC(C/T)GACTA downstream-variant-500B

Structural Variations from Database of Genomic Variants (DGV) for MYL12B Gene

Variant ID Type Subtype PubMed ID
nsv909317 CNV Gain 21882294
esv1749261 CNV Insertion 17803354
nsv131953 CNV Insertion 16902084

Relevant External Links for MYL12B Gene

HapMap Linkage Disequilibrium report
MYL12B

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MYL12B Gene

Disorders for MYL12B Gene

(1) University of Copenhagen DISEASES for MYL12B Gene

Relevant External Links for MYL12B

Genetic Association Database (GAD)
MYL12B
genes like me logo Genes that share disorders with MYL12B: view

No data available for UniProtKB/Swiss-Prot for MYL12B Gene

Publications for MYL12B Gene

  1. Diphosphorylated MRLC is required for organization of stress fibers in interphase cells and the contractile ring in dividing cells. (PMID: 11942626) Iwasaki T. … Hosoya H. (Cell Struct. Funct. 2001) 2 3 4
  2. Epigallocatechin-3-O-gallate disrupts stress fibers and the contractile ring by reducing myosin regulatory light chain phosphorylation mediated through the target molecule 67 kDa laminin receptor. (PMID: 15946647) Umeda D. … Yamada K. (Biochem. Biophys. Res. Commun. 2005) 3 4
  3. Functional specialization of beta-arrestin interactions revealed by proteomic analysis. (PMID: 17620599) Xiao K. … Lefkowitz R.J. (Proc. Natl. Acad. Sci. U.S.A. 2007) 3
  4. Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia. (PMID: 19165527) Martins-de-Souza D. … Turck C.W. (Eur Arch Psychiatry Clin Neurosci 2009) 3
  5. Nuclear myosin II regulates the assembly of preinitiation complex for ICAM-1 gene transcription. (PMID: 19328794) Li Q. … Sarna S.K. (Gastroenterology 2009) 3

Products for MYL12B Gene

Sources for MYL12B Gene

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