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Aliases for MYL12B Gene

Aliases for MYL12B Gene

  • Myosin Light Chain 12B 2 3 5
  • Myosin Regulatory Light Chain 2-B, Smooth Muscle Isoform 3 4
  • Myosin Regulatory Light Chain 20 KDa 3 4
  • Myosin, Light Chain 12B, Regulatory 2 3
  • Myosin Regulatory Light Chain MRLC2 3 4
  • Myosin Regulatory Light Chain 2 2 3
  • SHUJUN-1 3 4
  • MLC-2A 3 4
  • MLC-2 3 4
  • MLC20 3 4
  • MRLC2 3 4
  • MYLC2B 4
  • MLC-B 3

External Ids for MYL12B Gene

Previous GeneCards Identifiers for MYL12B Gene

  • GC18P003254

Summaries for MYL12B Gene

Entrez Gene Summary for MYL12B Gene

  • The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM, Mar 2008]

GeneCards Summary for MYL12B Gene

MYL12B (Myosin Light Chain 12B) is a Protein Coding gene. Among its related pathways are Focal Adhesion and Blood-Brain Barrier and Immune Cell Transmigration: VCAM-1/CD106 Signaling Pathways. GO annotations related to this gene include calcium ion binding. An important paralog of this gene is MYLPF.

UniProtKB/Swiss-Prot for MYL12B Gene

  • Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion (By similarity).

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MYL12B Gene

Genomics for MYL12B Gene

Regulatory Elements for MYL12B Gene

Enhancers for MYL12B Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around MYL12B on UCSC Golden Path with GeneCards custom track

Promoters for MYL12B Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around MYL12B on UCSC Golden Path with GeneCards custom track

Genomic Location for MYL12B Gene

Chromosome:
18
Start:
3,261,909 bp from pter
End:
3,278,284 bp from pter
Size:
16,376 bases
Orientation:
Plus strand

Genomic View for MYL12B Gene

Genes around MYL12B on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MYL12B Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MYL12B Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MYL12B Gene

Proteins for MYL12B Gene

  • Protein details for MYL12B Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O14950-ML12B_HUMAN
    Recommended name:
    Myosin regulatory light chain 12B
    Protein Accession:
    O14950
    Secondary Accessions:
    • D3DUH6
    • Q13182
    • Q7Z5Z4

    Protein attributes for MYL12B Gene

    Size:
    172 amino acids
    Molecular mass:
    19779 Da
    Quaternary structure:
    • Myosin is a hexamer of 2 heavy chains and 4 light chains.
    Miscellaneous:
    • This chain binds calcium.
    SequenceCaution:
    • Sequence=AAP73808.1; Type=Frameshift; Positions=4; Evidence={ECO:0000305};

neXtProt entry for MYL12B Gene

Proteomics data for MYL12B Gene at MOPED

Post-translational modifications for MYL12B Gene

  • Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for MYL12B Gene

No data available for DME Specific Peptides for MYL12B Gene

Domains & Families for MYL12B Gene

Gene Families for MYL12B Gene

Protein Domains for MYL12B Gene

Suggested Antigen Peptide Sequences for MYL12B Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

O14950

UniProtKB/Swiss-Prot:

ML12B_HUMAN :
  • Contains 3 EF-hand domains.
Domain:
  • Contains 3 EF-hand domains.
genes like me logo Genes that share domains with MYL12B: view

Function for MYL12B Gene

Molecular function for MYL12B Gene

UniProtKB/Swiss-Prot Function:
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion (By similarity).
genes like me logo Genes that share phenotypes with MYL12B: view

Animal Model Products

CRISPR Products

No data available for Enzyme Numbers (IUBMB) , Gene Ontology (GO) - Molecular Function , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for MYL12B Gene

Localization for MYL12B Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for MYL12B Gene COMPARTMENTS Subcellular localization image for MYL12B gene
Compartment Confidence
cytosol 5
extracellular 5
cytoskeleton 3
mitochondrion 2
endoplasmic reticulum 1
nucleus 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for MYL12B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005829 cytosol TAS --
GO:0005903 brush border IEA --
GO:0016460 myosin II complex IEA --
genes like me logo Genes that share ontologies with MYL12B: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for MYL12B Gene

Pathways & Interactions for MYL12B Gene

genes like me logo Genes that share pathways with MYL12B: view

SIGNOR curated interactions for MYL12B Gene

Is activated by:

Gene Ontology (GO) - Biological Process for MYL12B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006936 muscle contraction TAS --
GO:0007411 axon guidance TAS --
genes like me logo Genes that share ontologies with MYL12B: view

Drugs & Compounds for MYL12B Gene

(1) Drugs for MYL12B Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
calcium Nutra 0
genes like me logo Genes that share compounds with MYL12B: view

Transcripts for MYL12B Gene

Unigene Clusters for MYL12B Gene

Myosin, light chain 12B, regulatory:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for MYL12B Gene

No ASD Table

Relevant External Links for MYL12B Gene

GeneLoc Exon Structure for
MYL12B
ECgene alternative splicing isoforms for
MYL12B

Expression for MYL12B Gene

mRNA expression in normal human tissues for MYL12B Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for MYL12B Gene

This gene is overexpressed in Lavage (27.1), Bone marrow mesenchymal stem cell (10.3), Testis (7.0), Brain (6.3), and Fetal ovary (6.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for MYL12B Gene



SOURCE GeneReport for Unigene cluster for MYL12B Gene Hs.464472

mRNA Expression by UniProt/SwissProt for MYL12B Gene

O14950-ML12B_HUMAN
Tissue specificity: Ubiquitously expressed in various hematopoietic cells.
genes like me logo Genes that share expression patterns with MYL12B: view

Primer Products

In Situ Assay Products

No data available for mRNA differential expression in normal tissues and Protein tissue co-expression partners for MYL12B Gene

Orthologs for MYL12B Gene

This gene was present in the common ancestor of animals.

Orthologs for MYL12B Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia MYL12A 35
  • 90.12 (n)
  • 98.84 (a)
MYL12B 36
  • 99 (a)
OneToOne
dog
(Canis familiaris)
Mammalia MYL12A 35
  • 92.25 (n)
  • 98.84 (a)
MYL12B 36
  • 99 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Myl12a 35
  • 86.43 (n)
  • 97.67 (a)
Myl12a 16
Myl12b 36
  • 100 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia MYL12B 35
  • 99.81 (n)
  • 100 (a)
MYL12B 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Myl12a 35
  • 87.02 (n)
  • 97.67 (a)
oppossum
(Monodelphis domestica)
Mammalia MYL12B 36
  • 100 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 94 (a)
OneToMany
chicken
(Gallus gallus)
Aves LOC770011 35
  • 86.43 (n)
  • 98.84 (a)
-- 36
  • 99 (a)
ManyToMany
lizard
(Anolis carolinensis)
Reptilia -- 36
  • 97 (a)
ManyToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia myl12b 35
  • 81.98 (n)
  • 94.77 (a)
zebrafish
(Danio rerio)
Actinopterygii myl12.2 35
  • 79.84 (n)
  • 94.77 (a)
myl12.1 36
  • 92 (a)
ManyToMany
myl12.2 36
  • 95 (a)
ManyToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP000927 35
  • 73.63 (n)
  • 82.91 (a)
fruit fly
(Drosophila melanogaster)
Insecta sqh 35
  • 75.53 (n)
  • 84.18 (a)
sqh 36
  • 81 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea mlc-4 35
  • 70.46 (n)
  • 75.95 (a)
mlc-4 36
  • 72 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.5139 36
  • 80 (a)
OneToMany
Species with no ortholog for MYL12B:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for MYL12B Gene

ENSEMBL:
Gene Tree for MYL12B (if available)
TreeFam:
Gene Tree for MYL12B (if available)

Paralogs for MYL12B Gene

Paralogs for MYL12B Gene

(20) SIMAP similar genes for MYL12B Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with MYL12B: view

Variants for MYL12B Gene

Sequence variations from dbSNP and Humsavar for MYL12B Gene

SNP ID Clin Chr 18 pos Sequence Context AA Info Type
rs14720 - 3,277,840(+) GGATG(A/G)GCTGT reference, missense
rs1630702 -- 3,267,810(+) GAGGG(C/T)TGGTT intron-variant
rs1662332 -- 3,265,234(-) ATCTA(C/T)CTAAT intron-variant
rs1662333 -- 3,265,139(-) CTCAA(C/T)TTTTT intron-variant
rs1785243 -- 3,274,255(+) CCAAA(C/G)ATTGA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for MYL12B Gene

Variant ID Type Subtype PubMed ID
nsv909317 CNV Gain 21882294
esv1749261 CNV Insertion 17803354
nsv131953 CNV Insertion 16902084

Variation tolerance for MYL12B Gene

Residual Variation Intolerance Score: 47.3% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for MYL12B Gene

HapMap Linkage Disequilibrium report
MYL12B

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MYL12B Gene

Disorders for MYL12B Gene

Relevant External Links for MYL12B

Genetic Association Database (GAD)
MYL12B
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
MYL12B

No disorders were found for MYL12B Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for MYL12B Gene

Publications for MYL12B Gene

  1. Diphosphorylated MRLC is required for organization of stress fibers in interphase cells and the contractile ring in dividing cells. (PMID: 11942626) Iwasaki T. … Hosoya H. (Cell Struct. Funct. 2001) 2 3 4 67
  2. Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes. (PMID: 25609649) Li X. … Chen J. (Mol. Syst. Biol. 2015) 3
  3. Quantitative Proteomics Reveals Dynamic Interactions of the Minichromosome Maintenance Complex (MCM) in the Cellular Response to Etoposide Induced DNA Damage. (PMID: 25963833) Drissi R. … Boisvert F.M. (Mol. Cell Proteomics 2015) 3
  4. Genome-wide data reveal novel genes for methotrexate response in a large cohort of juvenile idiopathic arthritis cases. (PMID: 24709693) Cobb J. … Thomson W. (Pharmacogenomics J. 2014) 3
  5. Characterization of LGALS3 (galectin-3) as a player in DNA damage response. (PMID: 24755837) Carvalho R.S. … Carvalho M.A. (Cancer Biol. Ther. 2014) 3

Products for MYL12B Gene

Sources for MYL12B Gene

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