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MUC6 Gene

protein-coding   GIFtS: 56

GC11M001002
mucin 6, oligomeric mucus/gel-forming
(Previous names: mucin 6, gastric )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
MUC-6 3
Descriptions
Gastric mucin-6 3
mucin 6, gastric 1, 2
mucin 6, oligomeric mucus/gel-forming 2
External Ids
HGNC: 75171
Entrez Gene: 45882
UniProtKB: Q6W4X93
Ensembl: ENSG000001849567
Search outside databases for aliases for MUC6 gene

Previous GC identifers: GC11U990090 GC11P000176 GC11M001008 GC11M000974

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: MUC6_HUMAN, Q6W4X9
Function: May provide a mechanism for modulation of the composition of the protective mucus layer
related to acid secretion or the presence of bacteria and noxious agents in the lumen. Play an
important role in the cytoprotection of epithelial surfaces and are used as tumor markers in a
variety of cancers. May play a role in epithelial organogenesis

Gene Wiki entry for MUC6

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the MUC6 gene  

Entrez Gene cytogenetic band: 11p15.5-p15.4   Ensembl cytogenetic band:  11p15.5   HGNC cytogenetic band: 11p15.5

MUC6 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11M001002:     (about GC identifiers)

Start:
1,002,824 bp from pter
End:
1,026,706 bp from pter
Size:
23,883 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000011.8  NT_035113.6  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: MUC6_HUMAN, Q6W4X9 (See protein sequence)
Recommended Name: Mucin-6 precursor  
Size: 2392 amino acids; 252165 Da
Subunit: Multimer; disulfide-linked
Subcellular location: Secreted
Developmental stage: Early expressed in fetal development and was observed in Brunner's glands and
pancreatic ducts at 18-19 weeks and in gastric glands at 20 weeks of gestation. Expressed
transiently in the nephrogenic zone of the kidney in the early mid-trimester of development.
Detected in the epithelium of ureteric buds at 13 weeks and at lower levels from 17 to 23 weeks of
gestation
Sequence caution: Sequence=AK092533; Type=Erroneous termination; Positions=2310; Note=Translated as
Glu;
Secondary accessions: O15329 Q14394 Q2TUQ5 Q4L207 Q8N8I1 Q8NAK1

Post-translational modifications:

  • O-glycosylated1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_005952.2  

    ENSEMBL proteins: 
    ENSP00000371696 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Gastric Mucin )
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576 extracellular region NAS--
    About this table

    Antibodies for MUC6: 
    Browse Antibodies Central at Invitrogen
    Millipore Mono- and Polyclonal Antibodies for the study of MUC6
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Gastric Mucin ), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for MUC6

    Assays for MUC6: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR014853 Conserved-cysteine-rich_domain
     IPR018453 Prot_Inh_CR_TIL_sg
     IPR006207 Cys_knot_C
     IPR001846 von_Willebrand_fac_D


       GeneDecks  MUC6 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q6W4X9

    ProtoNet protein and cluster: Q6W4X9

    1 Blocks protein family: IPB002919 Trypsin inhibitor-like

    UniProtKB/Swiss-Prot: MUC6_HUMAN, Q6W4X9
    Similarity: Contains 1 CTCK (C-terminal cystine knot-like) domain
    Similarity: Contains 1 TIL (trypsin inhibitory-like) domain
    Similarity: Contains 3 VWFD domains

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (MUC6)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                    Browse Origene for 29mer shRNA kit in GFP-retroviral vector

                  Applied Biosystems Silencer® siRNAs for MUC6

                  Sigma-Aldrich siRNA for MUC6  
                         Sigma-Aldrich shRNA for MUC6  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005961
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005961
                                     untagged cDNA clone in CMV expression vector: NM_005961 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_005961

    UniProtKB/Swiss-Prot: MUC6_HUMAN, Q6W4X9
    Function: May provide a mechanism for modulation of the composition of the protective mucus layer
    related to acid secretion or the presence of bacteria and noxious agents in the lumen. Play an
    important role in the cytoprotection of epithelial surfaces and are used as tumor markers in a
    variety of cancers. May play a role in epithelial organogenesis
    Induction: Up-regulated by NFKB1. Repressed by mithramycin A which is an inhibitor of binding of
    transcription factors

    Genatlas biochemistry entry for MUC6:
    mucin 6,gastric,belonging to the secreted mucins cluster,aberrantly expressed in colorectal
    neoplasia

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005201 extracellular matrix structural constituent NAS7680650
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    5/14 Interacting proteins for MUC6 (ENSP000003716963) via UniProtKB, MINT, and/or STRING (see all 14 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TFF1ENSP000002915273STRING (score=.924)
    TFF3ENSP000002915253STRING (score=.897)
    MUC4ENSP000003105283STRING (score=.809)
    MUC19ENSP000003701953STRING (score=.794)
    MUC13ENSP000003122353STRING (score=.782)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0030277 maintenance of gastrointestinal epithelium NAS7680650
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for MUC6
    4 Novoseek chemical compound relationships for MUC6 gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    calcium 2.96 2 12517055 (2)
    oligonucleotide 0.00 1 11673830 (1)
    cholesterol 0.00 3 12517055 (2), 14634353 (1)
    progesterone 0.00 2 15086841 (1), 15737028 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (MUC6)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                    Browse Origene for 29mer shRNA kit in GFP-retroviral vector

                  Sigma-Aldrich siRNA for MUC6  
                         Sigma-Aldrich shRNA for MUC6  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_005961  

    REFSEQ mRNAs for MUC6 gene: 

    NM_005961.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_005961  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005961
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005961
                                     untagged cDNA clone in CMV expression vector: NM_005961 

    Additional cDNA sequence: 

    AK092533.1 AK096772.1 AK098503.1 AY312160.1 AY458429.1 U97698.1 

    9 DOTS entries:

    DT.97847590  DT.120730951  DT.95288353  DT.91682232  DT.120730948  DT.91750804  DT.95341118  DT.75101750 
    DT.87002890 

    24/83 AceView cDNA sequences (see all 83 ):

    BM769293 AI732669 BM856696 BM768868 BM798703 BM823251 AA588236 BM818289 
    BM767214 AA687898 BM769894 BQ084019 BQ082919 AW130323 AI420483 BM794541 
    BM773508 BM856626 BM773490 BM768831 AI868225 BM773506 BM831149 BQ082729 

    highest scoring ESTs for MUC6:

    AY312160 U97698 AA588236 AA603459 AI420483 AI468227 AI521662 AI732669 AI804760 AI868225 

    Unigene Cluster for MUC6:

    Mucin 6, oligomeric mucus/gel-forming
    Hs.528432  [show with all ESTs]
    Unigene Representative Sequence: NM_005961


    GeneLoc Exon Structure

    2 Alternative Splicing Database (ASD) splice patterns (SP) for MUC6

    ExUns: 1a · 1b ^ 2a · 2b ^ 3a · 3b
    SP1:                    -               
    SP2:              -                     

    About this scheme

    ECgene alternative splicing isoforms for MUC6

    1 Ensembl transcript including schematic representation:
    ENST00000382261  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    MUC6 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for MUC6

    1 / 2 / 3

    5 probe-sets matching MUC6 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    38217_at2, 3 U95-A 3 1.00 0.96 0.97 1.03 U97698 0.80 1.00 0.91 1
    707_s_at2, 3 U95-A 1 0.94 1.00 0.96 0.99 HG880-HT880 0.20 1.00 0.72 1

    214133_at2, 3 U133-A 1 1.00 1.00 -- -- AI611214 0.20 1.00 0.72 1

    214133_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1565666_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  MUC6 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GCGAACGTGA

    SOURCE GeneReport for Unigene cluster: Hs.528432

    UniProtKB/Swiss-Prot: MUC6_HUMAN, Q6W4X9
    Tissue specificity: Expressed in the regenerative zone of gastric antrum, gastric body mucosa and
    gastric incisura mucosa. Expressed in the deeper mucous glands of gastric antrum. Overexpressed in
    Helicobacter pylori infected gastric epithelium. Highly expressed in duodenal Brunner's glands,
    gall bladder, seminal vesicle, pancreatic centroacinar cells and ducts, and periductal glands of
    the common bile duct

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for MUC6 gene from 4 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    MUC61   -- mucin 6, oligomeric mucus/gel-forming 80.48(n)
    76.08(a)
    483652  XM_540773.2  XP_540773.2 
    chimpanzee
    (Pan troglodytes)
    MUC61   -- mucin 6, oligomeric mucus/gel-forming 97.57(n)
    96.01(a)
    740824  XM_001152213.1  XP_001152213.1 
    mouse
    (Mus musculus)
    Muc61, 5 7 (68.98 cM)5
    mucin 6, gastric1, 5 66.88(n)1
    60.8(a)1
    3533281  NM_181729.11  NP_859418.11 
     AF5335265  AJ0107525  (see all 14)
    chicken
    (Gallus gallus)
    MUC61   -- mucin 6, oligomeric mucus/gel-forming 63.09(n)
    63.94(a)
    414878  XM_426405.2  XP_426405.2 
    About this table        Species with no ortholog for MUC6

    ENSEMBL Gene Tree for MUC6
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for MUC6 gene
    MUC22  VWF2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section

    UniProtKB/Swiss-Prot: MUC6_HUMAN, Q6W4X9
    Polymorphism: The number of repeats is highly polymorphic and varies among different alleles. These
    repeats are very similar but not identical


    10/160 NCBI SNPs in MUC6 are shown (see all 160 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 38)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 11 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs65979461,2
    A,C,F,H1027888(-) TGGGAC/TGGGGG 1 -- ng314Minor allele frequency- T:0.26EU EA WA 374
    rs74815211,2
    A,C,F,H1017811(-) GGTGCG/ATGTAC 1 M/V mis1 ese38Minor allele frequency- A:0.21EU EA WA 838
    rs116053031,2
    A,C,F,H1028110(+) AGTACG/ATGTGC 1 -- ng318Minor allele frequency- A:0.05EU EA WA 840
    rs65979471,2
    C,F,H1027029(-) GGCGTG/TTGGCT 1 -- ng317Minor allele frequency- T:0.12EU EA WA NA 558
    --
    rs32088101,2
    C,F,H1006724(-) CATCAT/CCCCTA 1 T/I mis15Minor allele frequency- C:0.22MN EU EA WA 590
    rs116047571,2
    C,F,H1019320(+) GTGTCC/TGGGGC 1 G/R mis1 ese34Minor allele frequency- T:0.03EU EA WA 420
    --
    rs109022701,2
    A,C,H1008200(+) CAGGAT/AGGTGT 1 L/H mis1 ese34Minor allele frequency- A:0.00EU EA WA 418
    rs109022681,2
    A,C1003987(+) CTCCCA/GCACAC 1 R/W mis10--------
    --
    rs128070841,2
    F,H1008144(+) GGTGCG/CTGTAC 1 G/R mis1 ese34Minor allele frequency- C:0.01EU EA WA 404
    --
    rs116053201,2
    A,C1028199(+) tgtagA/Ggacga 1 -- ng310--------
    About this table

    HapMap Linkage Disequilibrium images for MUC6 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 158374

    10/37 Novoseek disease relationships for MUC6 gene (see all 37 )

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    gastric carcinoma 74.78 37 15737028 (4), 11745219 (3), 15354218 (2), 12719460 (2) (see all 21)
    metaplasia 73.60 27 10070955 (4), 14508397 (3), 16545139 (2), 11093805 (1) (see all 10)
    carcinoma mucinous 72.16 7 12493022 (4), 15976813 (1), 18493590 (1)
    adenocarcinoma 60.66 26 15753982 (5), 19094709 (2), 14623937 (2), 15086835 (2) (see all 15)
    carcinoma 57.37 17 15260848 (3), 11253133 (2), 15071739 (2), 16135257 (2) (see all 11)
    adenoma 55.34 15 9935202 (3), 17085661 (2), 15260848 (2), 12820724 (2) (see all 7)
    carcinoma bronchioloalveolar 53.72 5 11274635 (2), 15151204 (2)
    dysplasia 51.53 16 9935202 (3), 18097286 (2), 16949933 (2), 15260848 (2) (see all 7)
    carcinoma pancreatic ductal 50.97 1 16018800 (1)
    gastric polyp 49.61 2 10398119 (1), 10699992 (1)
    About this table

    Human Genome Epidemiology Navigator: MUC6 (2 documents)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/189 PubMed articles for MUC6 gene (see all 189 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 4588 HGNC: 7517 AceView: MUC6 Ensembl:ENSG00000184956 euGenes: HUgn4588
    ECgene: MUC6 H-InvDB: MUC6
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    Mucin databasehttp://www.medkem.gu.se/mucinbiology/databases/
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for MUC6:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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