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MRGPRD Gene

protein-coding GIFtS: 46

GC11M068504

MAS-related GPR, member D
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
MRGD ^², ³, ⁵
TGR7 ^²
mrgD ^¹

Descriptions
Beta-alanine receptor ^³
G-protein coupled receptor TGR7 ^³
MAS-related GPR, member D ^²
mas-related G protein-coupled MRGD ^²

External Ids
HGNC: 29626¹
Entrez Gene: 116512²
UniProtKB: Q8TDS7³
Ensembl: ENSG00000172938⁷

Search outside databases for aliases for MRGPRD gene

Previous GC identifer: GC11M068523

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: MRGRD_HUMAN, Q8TDS7

Function: May regulate nociceptor function and/or development, including the sensation or

modulation of pain. Functions as a specific membrane receptor for beta-alanine. Beta-alanine at

micromolar doses specifically evoked Ca(2+) influx in cells expressing the receptor. Beta-alanine

decreases forskolin-stimulated cAMP production in cells expressing the receptor, suggesting that

the receptor couples with G-protein G(q) and G(i)

Gene Wiki entry for MRGPRD

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the MRGPRD gene

Entrez Gene cytogenetic band: 11q13.2 Ensembl cytogenetic band: 11q13.2 HGNC cytogenetic band: 11q13.3

MRGPRD Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11 GeneLoc Exon Structure

GeneLoc location for GC11M068504: (about GC identifiers)

Start:	68,504,066 bp from pter
End:	68,505,031 bp from pter
Size:	966 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000011.8 NT_033903.7

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: MRGRD_HUMAN, Q8TDS7 (See protein sequence)

Recommended Name: Mas-related G-protein coupled receptor member D

Size: 321 amino acids; 36118 Da

Subcellular location: Cell membrane; Multi-pass membrane protein. Note=Localized at the plasma

membrane but internalized into the cytoplasm after treatment with beta-alanine

Secondary accessions: Q8NGK7

REFSEQ proteins: NP_944605.2

ENSEMBL proteins:

ENSP00000310631

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Millipore Purified and/or Recombinant MRGPRD Protein
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (GPCR TGR7)
	Human Recombinant Proteins from Abnova (MRGPRD)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005886	plasma membrane	IEA	--
GO:0016021	integral to membrane	IEA	--

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Antibodies for MRGPRD:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (GPCR TGR7), each with their Abpromise^SM
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for MRGPRD

Assays for MRGPRD:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q8TDS7

ProtoNet protein and cluster: Q8TDS7

UniProtKB/Swiss-Prot: MRGRD_HUMAN, Q8TDS7

Similarity: Belongs to the G-protein coupled receptor 1 family. Mas subfamily

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MRGPRD)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (MRGPRD)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_198923

Applied Biosystems Silencer® siRNAs for MRGPRD

Sigma-Aldrich siRNA and siRNA Panels for MRGPRD

Sigma-Aldrich shRNA for MRGPRD

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Millipore Clones for the Expression of MRGPRD

OriGene GFP tagged cDNA clone in CMV expression vector: NM_198923
Myc/DDK tagged cDNA clone in CMV expression vector: NM_198923
untagged cDNA clone in CMV expression vector: NM_198923

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_198923

UniProtKB/Swiss-Prot: MRGRD_HUMAN, Q8TDS7

Function: May regulate nociceptor function and/or development, including the sensation or

modulation of pain. Functions as a specific membrane receptor for beta-alanine. Beta-alanine at

micromolar doses specifically evoked Ca(2+) influx in cells expressing the receptor. Beta-alanine

decreases forskolin-stimulated cAMP production in cells expressing the receptor, suggesting that

the receptor couples with G-protein G(q) and G(i)

1 MGI mutant phenotype (inferred from 1 allele) (MGI details for Mrgprd):

normal

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0004872	receptor activity	IEA	--
GO:0004930	G-protein coupled receptor activity	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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2 Gene Ontology (GO) biological process terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0007165	signal transduction	IEA	--
GO:0007186	G-protein coupled receptor protein signaling pathway	IEA	--

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for MRGPRD
2 Novoseek chemical compound relationships for MRGPRD gene

Compound	Score	Articles	PubMed IDs for Articles with Shared Sentences (# sentences)
beta-alanine	86.63	11	15037633 (5), 17442834 (3), 17184592 (1), 8211147 (1)
calcium	9.59	5	15037633 (2), 17184592 (2)

About this table

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MRGPRD)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (MRGPRD)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_198923

Sigma-Aldrich siRNA and siRNA Panels for MRGPRD

Sigma-Aldrich shRNA for MRGPRD

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_198923

REFSEQ mRNAs for MRGPRD gene:

NM_198923.2

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_198923

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_198923
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_198923
                                 untagged cDNA clone in CMV expression vector: NM_198923

Additional cDNA sequence:

AB154410.1 AY427820.1

1 DOTS entry:

DT.101975814

3 AceView cDNA sequences:

NM_198923 AY427820 AB154410

highest scoring ESTs for MRGPRD:

AB154410 AY427820 NM_198923 CO923737

Unigene Cluster for MRGPRD:

MAS-related GPR, member D

Hs.527802

Unigene Representative Sequence: AY427820

GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:

ENST00000309106

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

MRGPRD expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for MRGPRD

¹ Data from GNF BioGPS
About these images

About these images

CGAP SAGE TAG: GGGGTCTTAA

SOURCE GeneReport for Unigene cluster: Hs.527802

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for MRGPRD gene from 5 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	MRGPRD¹	--	MAS-related GPR, member D	76.88(n) 66.2(a)	483685 XM_540806.1 XP_540806.1
chimpanzee (Pan troglodytes)	MRGPRD¹	--	MAS-related GPR, member D	98.65(n) 96.55(a)	466686 XM_522085.2 XP_522085.2
cow (Bos taurus)	MRGPRD¹	--	MAS-related GPR, member D	69.76(n) 54.49(a)	615853 XM_867774.2 XP_872867.2
rat (Rattus norvegicus)	Mrgprd¹	--	MAS-related GPR, member D	73.59(n) 63.67(a)	293648 NM_001001506.1 NP_001001506.1
mouse (Mus musculus)	Mrgprd^{1, 5}	7⁵	MAS-related GPR, member D^{1, 5}	69.62(n)¹ 59.81(a)¹	211578¹ NM_203490.3¹ NP_987075.1¹ AB154412⁵ AY042209⁵ (see all 8)

About this table Species with no ortholog for MRGPRD

ENSEMBL Gene Tree for MRGPRD

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for MRGPRD gene

MAS1² MRGPRF²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/14 NCBI SNPs in MRGPRD are shown (see all 14 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 26)

AB	SNP ID	Valid	Chr 11 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs4930634^1,2	A,C,F,H	68505779(+)	`CAAAGT/AGTAGA`	1	--	ng3¹ tfbs³		12		EU EA WA NA	1150
	rs10896390^1,2	A,C,F,H	68506116(+)	`TCTGTC/TATCCA`	1	--	ng3¹		8		EU EA WA	836
	rs7926802^1,2	H	68505127(+)	`GCTCTC/TCCCTC`	1	--	ng3¹ trp³		4		EU EA WA	418
--	rs7926571^1,2	C	68504956(+)	`GTAGGC/TCGTGT`	1	A/T	mis¹ ese³		1		EA	170
	rs1939734^1,2	C	68504061(+)	`TGGGCA/GGCTCT`	1	--	ng5¹		0	--	--	--	--
--	rs34900926^1,2	--	68505746(+)	`CTCTT-/AAAAAA`	1	--	ng3¹		0	--	--	--	--
--	rs34283108^1,2	--	68506922(+)	`CCTCC-/AATAGT`	1	--	ng3¹		0	--	--	--	--
--	rs71968866^1,2	--	68505752(+)	`AAAAA-/AAAAAA`	1	--	ng3¹		0	--	--	--	--
--	rs7129887^1,2	--	68504157(+)	`TCCCCA/GGGGAC`	1	L/P	mis¹ ese³		0	--	--	--	--
--	rs56802110^1,2	--	68505697(+)	`CATGGA/TCCCGC`	1	--	ng3¹		0	--	--	--	--

About this table

HapMap Linkage Disequilibrium images for MRGPRD (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 607231

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

8 PubMed articles for MRGPRD gene:

Atypical expansion in mice of the sensory neuron-specific Mrg G protein-coupled receptor family. (PubMed id 12909716)^{2, 3, 4} Zylka M.J....Anderson D.J. (2003)
Identification of a G protein-coupled receptor specifically responsive to beta-alanine. (PubMed id 15037633)^{1, 3, 4} Shinohara T.... Hinuma S. (2004)
Identification of G protein-coupled receptor genes from the human genome sequence. (PubMed id 12044878)^{3, 4} Takeda S.... Mitaku S. (2002)
A diverse family of GPCRs expressed in specific subsets of nociceptive sensory neurons. (PubMed id 11551509)^{2, 3} Dong X.... Anderson D.J. (2001)
MrgD activation inhibits KCNQ/M-currents and contributes to enhanced neuronal excitability. (PubMed id 17442834)¹ Crozier R.A....Pausch M.H. (2007)
Angiotensin metabolites can stimulate receptors of the Mas-related genes family. (PubMed id 18636314)¹ Gembardt F....Walther T. (2008)
Development of a homogeneous calcium mobilization assay for high throughput screening of mas-related gene receptor agonists. (PubMed id 17184592)¹ Zhang R....Wang M.W. (2007)
Mutation of glycine receptor subunit creates beta-alanine receptor responsive to GABA. (PubMed id 8211147)¹ Schmieden V....Betz H. (1993)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 116512	HGNC: 29626	AceView: MRGPRD	Ensembl:ENSG00000172938	euGenes: HUgn116512
ECgene: MRGPRD	H-InvDB: MRGPRD

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for MRGPRD
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for MRGPRD:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for MRGPRD-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for MRGPRD		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for MRGPRD		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

	Recombinant Proteins (MRGPRD)
	Search for Antibodies
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	Custom Service for Mouse Mab
	Custom Service for Rabbit Pab from Full-length Protein

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	Recombinant Proteins (GPCR TGR7)
	Antibodies (GPCR TGR7)

Search OriGene for MRGPRD

Search Tocris compounds for MRGPRD

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Search Novus for antibodies for MRGPRD

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MRGPRD Gene

protein-coding GIFtS: 46

Aliases &
Descriptions
for MRGPRD

Summaries
for MRGPRD

Genomic Location
for MRGPRD

Proteins
for MRGPRD

Protein Domains/
Families
for MRGPRD

Gene Function
for MRGPRD

Pathways & Interactions
for MRGPRD

Drugs & Compounds
for MRGPRD

Transcripts
for MRGPRD

Expression
for MRGPRD

Orthologs
for MRGPRD

Paralogs
for MRGPRD

SNPs/Variants
for MRGPRD

Disorders & Mutations
for MRGPRD

Medical News
for MRGPRD

Publications
for MRGPRD

Search for MRGPRD

Genome Databases showing MRGPRD

Other Databases showing MRGPRD

Specialized Databases showing MRGPRD

Licensable Technologies
for MRGPRD

Services
for MRGPRD

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

MRGPRD Gene

protein-coding GIFtS: 46

Aliases & Descriptionsfor MRGPRD

Summariesfor MRGPRD

Genomic Locationfor MRGPRD

Proteinsfor MRGPRD

Protein Domains/Familiesfor MRGPRD

Gene Functionfor MRGPRD

Pathways & Interactionsfor MRGPRD

Drugs & Compoundsfor MRGPRD

Transcriptsfor MRGPRD

Expression for MRGPRD

Orthologsfor MRGPRD

Paralogsfor MRGPRD

SNPs/Variantsfor MRGPRD

Disorders & Mutationsfor MRGPRD

Medical Newsfor MRGPRD

Publicationsfor MRGPRD

Search for MRGPRD

Genome Databases showing MRGPRD

Other Databases showing MRGPRD

Specialized Databases showing MRGPRD

Licensable Technologiesfor MRGPRD

Servicesfor MRGPRD

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for MRGPRD

Summaries
for MRGPRD

Genomic Location
for MRGPRD

Proteins
for MRGPRD

Protein Domains/
Families
for MRGPRD

Gene Function
for MRGPRD

Pathways & Interactions
for MRGPRD

Drugs & Compounds
for MRGPRD

Transcripts
for MRGPRD

Expression
for MRGPRD

Orthologs
for MRGPRD

Paralogs
for MRGPRD

SNPs/Variants
for MRGPRD

Disorders & Mutations
for MRGPRD

Medical News
for MRGPRD

Publications
for MRGPRD

Licensable Technologies
for MRGPRD

Services
for MRGPRD