MRFAP1 Gene
protein-coding GIFtS : 46
GCID: GC04 P006573
Morf4 family associated protein 1
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Aliasesfor MRFAP1 gene
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc ,
7 Ensembl ,
8 DME ,
9 miRBase ,
and/or 10 fRNAdb )About This Section Aliases Morf4 Family Associated Protein 1 1 2 Mof4 Family Associated Protein 12 PAM141 2 3 MORF4 Family-Associated Protein 12 PGR11 2 3 T-Cell Activation Protein2 Protein Associated With MRG Of 14 KDa2 3 Protein PGR13
Export aliases for MRFAP1 gene to outside databases Previous GC identifers: GC04P006761 GC04P006692 GC04P006643
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Summariesfor MRFAP1 gene (According to Entrez Gene ,
Tocris Bioscience ,
Wikipedia's
Gene Wiki ,
PharmGKB ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
About This Section Entrez Gene summary for MRFAP1 : This gene encodes an intracellular protein that interacts with members of the MORF4/MRG (mortality factor on chromosome 4/MORF4 related gene) family and the tumor suppressor Rb (retinoblastoma protein.) The protein may play a role in senescence, cell growth and immortalization. Alternative splicing results in multiple transcript variants. (provided by RefSeq, Jan 2013) Gene Wiki entry for MRFAP1
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Genomic Viewsfor MRFAP1 gene
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 37) ,
and/or miRBase ,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69) ,
Regulatory elements and Epigenetics data according to
QIAGEN ,
SABiosciences , and/or
SwitchGear Genomics )About This Section RefSeq DNA sequence: NC_000004.11 NC_018915.1 NT_006051.18 Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the MRFAP1 gene promoter: SRF SRF (504 AA) POU3F2 (N-Oct-5a) POU3F2 (N-Oct-5b) POU3F2 CBF-A POU2F1 POU2F1a HFH-1 CBF(2) Other transcription factors Search SABiosciences Chromatin IP Primers for MRFAP1 Epigenetics: QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human , mouse , rat MRFAP1
Genomic Location: Genomic View : UCSC Golden Path with GeneCards custom track Entrez Gene cytogenetic band: 4p16.1 Ensembl cytogenetic band: 4p16.1 HGNC cytogenetic band: 4p16.1 MRFAP1 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc information about chromosome 4 GeneLoc Exon Structure
GeneLoc location for GC04P006573: view genomic region
(about GC identifiers )
Start:
6,641,818 bp from pter
End:
6,644,472 bp from pter
Size:
2,655 bases
Orientation:
plus strand
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Proteinsfor MRFAP1 gene
(According to
1 UniProtKB ,
HORDE ,
neXtProt ,
Ensembl ,
and/or Reactome ,
Modification sites according to 2 PhosphoSitePlus ,
Specific Peptides from DME ,
Protein expression images according to data from SPIRE MOPED and PaxDb ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from
EMD Millipore ,
R&D Systems ,
GenScript ,
Enzo Life Sciences ,
OriGene ,
Novus Biologicals ,
Sino Biological ,
ProSpec , and/or
Uscn ,
Biochemical Assays by
EMD Millipore ,
R&D Systems ,
OriGene ,
GenScript ,
Cell Signaling Technology ,
Enzo Life Sciences , and/or
Uscn ,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene ,
Antibodies by
EMD Millipore ,
R&D Systems ,
GenScript ,
Cell Signaling Technology ,
OriGene ,
Novus Biologicals ,
Thermo Fisher Scientific ,
Abcam , and/or
Uscn )
About This Section UniProtKB/Swiss-Prot: MOFA1_HUMAN, Q9Y605 (See
protein sequence )Recommended Name: MORF4 family-associated protein 1 Size : 127 amino acids; 14650 Da
Subunit : Found in a complex composed of MORF4L1, MRFAP1 and RB1. Interacts via its N-terminus with MORF4L1. Interactswith CSTB and MORF4L2
Subcellular location : Nucleus. Cytoplasm, perinuclear region. Note=Colocalizes with MORF4L1 to cell nuclei
Secondary accessions : B3KVT2 D3DVT3Explore the universe of human proteins at neXtProt for MRFAP1: NX_Q9Y605 Post-translational modifications:
View modification sites using PhosphoSitePlus 2 View neXtProt modification sites for NX_Q9Y605 MRFAP1 Protein expression data from MOPED and PaxDb : About this image
REFSEQ proteins (3 alternative transcripts):
NP_001258982.1 NP_001258983.1 NP_150638.1 ENSEMBL proteins: ENSP00000318352 ENSP00000422065 ENSP00000372024 Human Recombinant Protein Products: Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view) : About this table
GO ID Qualified GO term Evidence PubMed IDs GO:0005634 nucleus
IEA -- GO:0048471 perinuclear region of cytoplasm
IEA --
MRFAP1 for ontologies About GeneDecksing MRFAP1 Antibody Products: Assay Products for MRFAP1:
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Protein
Domains / Familiesfor MRFAP1 gene (According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
About This Section ProtoNet protein and cluster: Q9Y605
UniProtKB/Swiss-Prot: MOFA1_HUMAN, Q9Y605 Similarity : Belongs to the MORF4 family-associated protein family
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Functionfor MRFAP1 gene
(According to 1 UniProtKB ,
Genatlas ,
LifeMap Discovery™ ,
IUBMB , and/or
2 DME ,
Human phenotypes from GenomeRNAi ,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences ,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene ,
RNAi from
EMD Millipore ,
siRNAs from
OriGene ,
QIAGEN ,
microRNA from QIAGEN ,
Gene Editing from DNA2.0 ,
Clones from EMD Millipore ,
OriGene ,
SwitchGear Genomics ,
GenScript ,
Sino Biological ,
DNA2.0 ,
and Vector BioLabs ,
Cell Lines from GenScript ,
LifeMap BioReagents ,
In Situ Hybridization Assays from Advanced Cell Diagnostics ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene .)
About This Section Clone Products: Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for MRFAP1 (see all 3 ) OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for MRFAP1OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector: MRFAP1 (NM_033296 ) Browse Sino Biological Human cDNA Clones DNA2.0 Custom Codon Optimized Gene
Synthesis Service for MRFAP1 Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat MRFAP1
In Situ Assay Products: Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MRFAP1
Gene Ontology (GO): 1 molecular function term (GO ID links to tree view) : About this table
MRFAP1 for ontologies About GeneDecksing Animal Models: Mouse knock-out Mrfap1 tm1Kto for MRFAP1 2 MGI mutant phenotypes (inferred from 1 allele ) (MGI details for Mrfap1) :
MRFAP1 for phenotypes About GeneDecksing
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Pathways & Interactionsfor MRFAP1 gene
(Pathways according to
EMD Millipore ,
R&D Systems ,
Cell Signaling Technology ,
KEGG ,
PharmGKB ,
BioSystems ,
Reactome ,
Tocris Bioscience ,
GeneGo (Thomson Reuters) ,
QIAGEN ,
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Interaction Networks according to
SABiosciences ,
and/or STRING ,
Interactions according to 1 UniProtKB ,
2 MINT ,
3 I2D , and/or
4 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene) .
About This Section Interactions: SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for MRFAP1 STRING Interaction
Network Preview (showing 5 interactants - click image to see 9)5/16 Interacting proteins for MRFAP1 (Q9Y605 2 , 3 ENSP00000318352 4 ) via UniProtKB, MINT, STRING , and/or I2D (see all 16 )About this table
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Drugs & Compoundsfor MRFAP1 gene (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
EMD Millipore , Tocris Bioscience
HMDB ,
BitterDB , and/or
Novoseek , and Drugs according to
DrugBank ,
Enzo Life Sciences , and/or
PharmGKB , with drugs/clinical trials/news
search links to CenterWatch )
About This Section Browse Tocris compounds for MRFAP1 Search CenterWatch for drugs/clinical trials and news about MRFAP1 / MOFA1
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Transcriptsfor MRFAP1 gene (Secondary structures according to
fRNAdb ,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
transcript ids from Ensembl
with links to UCSC ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from
EMD Millipore ,
siRNAs from
OriGene ,
QIAGEN ,
shRNA from
OriGene ,
microRNA from QIAGEN ,
Tagged/untagged cDNA clones from
OriGene ,
SwitchGear Genomics ,
GenScript ,
DNA2.0 ,
Vector BioLabs ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN
)About This Section REFSEQ mRNAs for MRFAP1 gene (3 alternative transcripts): NM_001272053.1 NM_001272054.1 NM_033296.2 Unigene Cluster for MRFAP1:
Morf4 family associated protein 1 Hs.727355 [show with all ESTs ] Unigene Representative Sequence: AK123360 4 Ensembl transcripts including schematic representations, and UCSC links where relevant : ENST00000320912 (uc003gjg.1 ) ENST00000507420 ENST00000382581 (uc003gjh.1 )ENST00000512914 (uc021xla.1 ) Clone Products: OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for MRFAP1 (see all 3 ) OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for MRFAP1OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector: MRFAP1 (NM_033296 ) DNA2.0 Custom Codon Optimized Gene
Synthesis Service for MRFAP1 Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat MRFAP1
Additional cDNA sequence: AK057736.1 AK074496.1 AK123360.1 AK129651.1 AK300242.1 AK303974.1 BC022797.1 CR457144.1
23 DOTS entries : DT.211720 DT.97791252
DT.100855508 DT.100687506 DT.100672233 DT.92469254 DT.100855010 DT.102833531 DT.100687515 DT.121243303 DT.100038649 DT.121243255 DT.92469220 DT.121243250 DT.95189941 DT.100687510 DT.121243246 DT.95371130 DT.95371140 DT.95371142 DT.100687508 DT.121243287 DT.91959457 24/602 AceView cDNA sequences (see all 602 ):
CR620538 BG287807 BM800252 AI126461 BM541441 CB124646 CD245522 AL540808 CB155573 BM466792 BI858568 CR618277 CD359740 BX399712 CD106860 AL537309 BM787269 BU153743 AI866614 CR619436 BG676156 CB141736 BM905112 BQ268716 GeneLoc Exon Structure 5/6 Alternative Splicing Database (ASD) splice patterns (SP) for MRFAP1 (see all 6 ) About this scheme ExUns: 1a · 1b · 1c ^ 2 ^ 3a · 3b ^ 4a · 4b ^ 5a · 5b · 5c · 5d · 5e SP1 :     -                       SP2 :                           SP3 :                           SP4 :           -   -               SP5 :                          
ECgene alternative splicing isoforms for MRFAP1
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Expression for MRFAP1 gene
(RNA expression data according to
H-InvDB ,
NONCODE ,
miRBase , and
RNAdb ,
Expression images according to data from
BioGPS ,
Illumina Human BodyMap , and
CGAP
SAGE ,
Sets of similar genes according to GeneDecks ,
in vivo and in vitro expression data from LifeMap Discovery™ ,
plus additional links to
Genevestigator , and/or
SOURCE , and/or
BioGPS , and/or
UniProtKB ,
PCR Arrays from
SABiosciences ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN ,
In Situ Hybridization Assays from Advanced Cell Diagnostics )
About This Section MRFAP1 expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this image BioGPS CGAP TAG: --
About this image MRFAP1 expression in embryonic tissues and stem cells Expression by the Database of Embryonic development, Stem cell research, and
Regenerative medicine About this table 1 LifeMap In Vivo Development Anatomical Compartment/Cell Tissue Anatomical Compartment
Cell Category (developmental path) Kidney Metanephric Mesenchyme Metanephric Mesenchyme Cells Kidney Expression: Positive Negative
Selective markerExperimental details:
Curated
Microarrays
In-situ hybridization
See MRFAP1 Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for MRFAP1 SOURCE GeneReport for Unigene cluster: Hs.727355 SABiosciences Custom PCR Arrays for MRFAP1 Primer Products: OriGene genome-wide validated SYBR primer pairs in human , mouse , rat for MRFAP1Browse OriGene validated miRNA SYBR primer pairs SABiosciences RT2 qPCR Primer Assay in human , mouse , rat MRFAP1 QIAGEN QuantiTect SYBR Green Assays in human , mouse , rat MRFAP1 QIAGEN QuantiFast Probe-based Assays in human , mouse , rat MRFAP1 In Situ Assay Products: Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MRFAP1
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Orthologsfor MRFAP1 gene
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
,
5 MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase ,
and/or
6 Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam )
About This Section
--
ENSEMBL Gene Tree for MRFAP1 (if available)TreeFam Gene Tree for MRFAP1 (if available)
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Paralogsfor MRFAP1 gene (Paralogs according to
1 HomoloGene , 2 Ensembl , and 3 SIMAP , Pseudogenes according to 4 Pseudogene.org Build 68)About This Section Paralogs for MRFAP1 gene MRFAP1L1 2 1 SIMAP similar gene for MRFAP1 using alignment to 1 protein entry: MOFA1_HUMAN :MRFAP1L1
MRFAP1 for paralogs About GeneDecksing 1 Pseudogenes.org Pseudogene for MRFAP1 PGOHUM00000260999
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Genomic Variantsfor MRFAP1 gene (SNPs/Variants according to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE ,
UniProtKB , and
DNA2.0 ,
Linkage Disequilibrium by HapMap ,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants , Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB) , Blood group antigen gene mutations by BGMUT ,
Resequencing Primers from QIAGEN ,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences )
About This Section SABiosciences Cancer Mutation PCR Assays
QIAGEN SeqTarget long-range PCR primers in human , mouse , rat for resequencing MRFAP1
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Disorders
/ Diseasesfor MRFAP1 gene
(in which this Gene is Involved, According to MalaCards ,
OMIM, UniProtKB ,
the University of Copenhagen DISEASES
database ,
Genatlas , GeneTests ,
GAD ,
HuGE Navigator ,
and/or TGDB .)
About This Section
MRFAP1 for disorders About GeneDecksing 3 diseases for MRFAP1 : About MalaCards cervix uteri carcinoma in situ retinoblastoma carcinoma Export disorders for MRFAP1 gene to outside databases
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Publicationsfor MRFAP1 gene (in
PubMed .
Associations of this gene to articles via
1 Entrez Gene ,
2 UniProtKB/Swiss-Prot ,
3 HGNC ,
4 GAD ,
5 PharmGKB ,
6 HMDB ,
7 DrugBank ,
8 UniProtKB/TrEMBL ,
9 Novoseek , and/or
10 fRNAdb )
About This Section PubMed articles for MRFAP1 gene, integrated from 9 sources (see all 26 ): (articles sorted by number of sources associating them with MRFAP1) Utopia : connect your pdf to the dynamic world of online information
The MRG domain of human MRG15 uses a shallow hydrophobic pocket to interact with the N-terminal region of PAM14. (PubMed id 17008723) 1 , 2 Zhang P.... Ding J. (2006) Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039) 1 , 2 Ota T.... Sugano S. (2004) MRGX is a novel transcriptional regulator that exhibits activation or repression of the B-myb promoter in a cell type-dependent manner. (PubMed id 14506250) 1 , 2 Tominaga K....Pereira-Smith O.M. (2003) MRG15, a novel chromodomain protein, is present in two distinct multiprotein complexes involved in transcriptional activation. (PubMed id 12397079) 1 , 2 Pardo P.S.... Pereira-Smith O.M. (2002) MRG15 activates the B-myb promoter through formation of a nuclear complex with the retinoblastoma protein and the novel protein PAM14. (PubMed id 11500496) 1 , 2 Leung J.K....Pereira-Smith O.M. (2001) Characterization of MRFAP1 turnover and interactions d ownstream of the NEDD8 pathway. (PubMed id 22038470) 1 Larance M....Lamond A.I. (2012) Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724) 1 Udeshi N.D....Carr S.A. (2012) Initial characterization of the human central proteome. (PubMed id 21269460) 2 Burkard T.R.... Colinge J. (2011) A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473) 1 Wagner S.A....Choudhary C. (2011) A directed protein interaction network for investigat ing intracellular signal transduction. (PubMed id 21900206) 1 Vinayagam A....Wanker E.E. (2011)
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External Searches for MRFAP1 gene
(in PubMed ,
OMIM , and NCBI Bookshelf ) About This Section
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Genome Databases showing MRFAP1 gene
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
Kegg ,
and/or
H-InvDB )
About This Section
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Other Databases showing MRFAP1 gene
(According to HUGE )
About This Section --
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Specialized Databases showing MRFAP1 gene (According to PharmGKB ,
ATLAS , HORDE , IMGT , LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
Name Description
PharmGKB entry for MRFAP1 Pharmacogenomics, SNPs, Pathways
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About This Section Patent Information for MRFAP1 gene: Search GeneIP for patents involving MRFAP1 GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
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Productsfor MRFAP1 gene (Antibodies, recombinant proteins, and assays from EMD Millipore , R&D Systems , OriGene , QIAGEN , GenScript , Cell Signaling Technology , SABiosciences , Novus Biologicals , Sino Biological , Enzo Life Sciences , Abcam , ProSpec , Uscn , Thermo Fisher Scientific , Gene Editing from DNA2.0 , Clones from EMD Millipore , OriGene , GenScript , Sino Biological , DNA2.0 , SwitchGear Genomics , Vector BioLabs , Cell lines from GenScript and LifeMap BioReagents , PCR Arrays from SABiosciences , Drugs and/or compounds from EMD Millipore , Tocris Bioscience , and/or
Enzo Life Sciences ), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
Browse OriGene Antibodies OriGene shRNA RFP for MRFAP1 OriGene 29mer shRNA kits in GFP-retroviral vector in human , mouse , rat for MRFAP1 OriGene genome-wide validated SYBR primer pairs in human , mouse , rat for MRFAP1 OriGene Protein Over-expression Lysate for MRFAP1 Browse OriGene Fluorogenic Cell Assay Kits OriGene siRNA for MRFAP1 OriGene 3'-UTR Clone for MRFAP1 OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for MRFAP1 OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for MRFAP1 Browse OriGene GFP tagged cDNA clones in CMV expression vector Browse OriGene MicroRNA Expression Plasmids Browse OriGene basic RS shRNAs Browse OriGene validated miRNA SYBR primer pairs OriGene Purified Protein for MRFAP1 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling OriGene Custom Antibody Services for MRFAP1 OriGene Custom Protein Services for MRFAP1 OriGene Custom Immunoassay Development
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat MRFAP1 QIAGEN SeqTarget long-range PCR primers in human , mouse , rat for resequencing MRFAP1 QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human , mouse , rat MRFAP1 QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human , mouse , rat MRFAP1 QIAGEN QuantiFast Probe-based Assays in human , mouse , rat MRFAP1 QIAGEN QuantiTect SYBR Green Assays in human , mouse , rat MRFAP1
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MRFAP1 Proteins, Antibodies, CLIAs, and ELISAs
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MRFAP1
ThermoFisher Antibody for MRFAP1
Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat MRFAP1
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