Free for academic non-profit institutions. Other users need a Commercial license

Aliases for MPST Gene

Aliases for MPST Gene

  • Mercaptopyruvate Sulfurtransferase 2 3 5
  • TRNA Thiouridin Modification Protein 1 2 3
  • Human Liver Rhodanese 2 3
  • TST2 3 4
  • MST 3 4
  • 3-Mercaptopyruvate Sulfurtransferase 3
  • Testicular Tissue Protein Li 200 3
  • EC 2.8.1.2 4
  • TUM1 3

External Ids for MPST Gene

Previous GeneCards Identifiers for MPST Gene

  • GC22P034030
  • GC22P035658
  • GC22P035740
  • GC22P037415
  • GC22P020380

Summaries for MPST Gene

Entrez Gene Summary for MPST Gene

  • This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

GeneCards Summary for MPST Gene

MPST (Mercaptopyruvate Sulfurtransferase) is a Protein Coding gene. Diseases associated with MPST include Mercaptolactate-Cysteine Disulfiduria and Cyanide Poisoning. Among its related pathways are Sulfur metabolism and Sulfur amino acid metabolism. GO annotations related to this gene include thiosulfate sulfurtransferase activity and 3-mercaptopyruvate sulfurtransferase activity. An important paralog of this gene is TST.

UniProtKB/Swiss-Prot for MPST Gene

  • Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions (By similarity).

Gene Wiki entry for MPST Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MPST Gene

Genomics for MPST Gene

Regulatory Elements for MPST Gene

Enhancers for MPST Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH22G037014 1.6 Ensembl ENCODE dbSUPER 18.8 +1.0 1039 12.5 HDGF PKNOX1 ARNT CREB3L1 ARID4B SIN3A DMAP1 ZNF2 YY1 ZNF766 MPST KCTD17 ANKRD54 ENSG00000231467 TMPRSS6 NCF4-AS1 NCF4 IL2RB ENSG00000229971 TST
GH22G037030 1.9 FANTOM5 Ensembl ENCODE dbSUPER 13.6 +13.9 13928 5.4 FOXA2 MLX ZFP64 ARID4B SIN3A DMAP1 ZNF2 ZBTB7B YY1 SLC30A9 TMPRSS6 MPST TEX33 ENSG00000231467 NCF4-AS1 NCF4 IL2RB ENSG00000229971 TST CARD10
GH22G036976 1.3 Ensembl ENCODE 8.3 -41.8 -41751 1.8 PKNOX1 ATF1 WRNIP1 ARID4B SIN3A YY1 GLIS2 ELK1 ZNF143 ZNF207 MPST TEX33 LL22NC01-81G9.3
GH22G037290 1.4 ENCODE dbSUPER 5.2 +271.1 271079 0.7 PKNOX1 CREB3L1 MLX ZFP64 ARID4B SIN3A DMAP1 FEZF1 ZNF2 YY1 RPS15AP38 MPST CYTH4 GC22M037293
GH22G037298 1.4 Ensembl ENCODE dbSUPER 5.1 +280.2 280233 3.6 HDGF RB1 SIN3A KLF17 RAD21 ZNF121 GLIS2 FOS SP3 ZNF362 CYTH4 MPST GC22M037293 LOC105373024
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around MPST on UCSC Golden Path with GeneCards custom track

Promoters for MPST Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000145924 -35 2001 HDGF ARNT CREB3L1 ARID4B SIN3A DMAP1 ZNF2 ZNF766 SP3 SP5

Genomic Location for MPST Gene

Chromosome:
22
Start:
37,019,635 bp from pter
End:
37,029,822 bp from pter
Size:
10,188 bases
Orientation:
Plus strand

Genomic View for MPST Gene

Genes around MPST on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MPST Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MPST Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MPST Gene

Proteins for MPST Gene

  • Protein details for MPST Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P25325-THTM_HUMAN
    Recommended name:
    3-mercaptopyruvate sulfurtransferase
    Protein Accession:
    P25325
    Secondary Accessions:
    • A8MZ34
    • B3KP52
    • J3KPV7
    • O75750
    • Q6FHN9

    Protein attributes for MPST Gene

    Size:
    297 amino acids
    Molecular mass:
    33178 Da
    Quaternary structure:
    • Monomer; active form. Homodimer; disulfide-linked, inactive form.
    Miscellaneous:
    • Thioredoxin (Trx) or dihydrolipoic acid (DHLA) are required to release hydrogen sulfide from the persulfide intermediate.
    SequenceCaution:
    • Sequence=AAH16737.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=CAG30409.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for MPST Gene

    Alternative splice isoforms for MPST Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MPST Gene

Post-translational modifications for MPST Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for MPST Gene

Domains & Families for MPST Gene

Gene Families for MPST Gene

Protein Domains for MPST Gene

Graphical View of Domain Structure for InterPro Entry

P25325

UniProtKB/Swiss-Prot:

THTM_HUMAN :
  • Contains two rhodanese domains with different primary structures but with near identical secondary structure conformations suggesting a common evolutionary origin. Only the C-terminal rhodanese domain contains the catalytic cysteine residue (By similarity).
Domain:
  • Contains two rhodanese domains with different primary structures but with near identical secondary structure conformations suggesting a common evolutionary origin. Only the C-terminal rhodanese domain contains the catalytic cysteine residue (By similarity).
genes like me logo Genes that share domains with MPST: view

Function for MPST Gene

Molecular function for MPST Gene

GENATLAS Biochemistry:
mercaptopyruvate sulfurtransferase
UniProtKB/Swiss-Prot CatalyticActivity:
3-mercaptopyruvate + cyanide = pyruvate + thiocyanate.
UniProtKB/Swiss-Prot EnzymeRegulation:
By oxidative stress, and thioredoxin. Under oxidative stress conditions, the catalytic cysteine site is converted to a sulfenate which inhibits the MPST enzyme activity. Reduced thioredoxin cleaves an intersubunit disulfide bond to turn on the redox switch and reactivate the enzyme.
UniProtKB/Swiss-Prot Function:
Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions (By similarity).

Enzyme Numbers (IUBMB) for MPST Gene

Gene Ontology (GO) - Molecular Function for MPST Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004792 thiosulfate sulfurtransferase activity IEA --
GO:0016740 transferase activity IEA --
GO:0016784 3-mercaptopyruvate sulfurtransferase activity IEA --
GO:0042802 identical protein binding IEA --
genes like me logo Genes that share ontologies with MPST: view
genes like me logo Genes that share phenotypes with MPST: view

Animal Models for MPST Gene

MGI Knock Outs for MPST:

Animal Model Products

  • Taconic Biosciences Mouse Models for MPST

miRNA for MPST Gene

miRTarBase miRNAs that target MPST

Inhibitory RNA Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for MPST Gene

Localization for MPST Gene

Subcellular locations from UniProtKB/Swiss-Prot for MPST Gene

Cytoplasm. Mitochondrion. Cell junction, synapse, synaptosome.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MPST gene
Compartment Confidence
extracellular 5
mitochondrion 5
cytosol 5
nucleus 2

Gene Ontology (GO) - Cellular Components for MPST Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IEA --
GO:0005739 mitochondrion IEA --
GO:0005829 cytosol IEA --
GO:0030054 cell junction IEA --
GO:0043005 neuron projection ISS --
genes like me logo Genes that share ontologies with MPST: view

Pathways & Interactions for MPST Gene

genes like me logo Genes that share pathways with MPST: view

Pathways by source for MPST Gene

Interacting Proteins for MPST Gene

Gene Ontology (GO) - Biological Process for MPST Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001822 kidney development IEA --
GO:0001889 liver development IEA --
GO:0009440 cyanate catabolic process TAS 9070219
GO:0009636 response to toxic substance TAS 9070219
GO:0019346 transsulfuration IEA --
genes like me logo Genes that share ontologies with MPST: view

No data available for SIGNOR curated interactions for MPST Gene

Drugs & Compounds for MPST Gene

(4) Drugs for MPST Gene - From: HMDB and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Pyruvic acid Approved Nutra 0

(9) Additional Compounds for MPST Gene - From: HMDB and Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
cyanide
  • Carbon nitride ion
  • Chuck Norrisium
  • Cyanide
  • Cyanide ion
  • Cyanide(1-) ion
57-12-5
thiocyanate
  • Rhodanide
  • SCN
  • Ammonium sulfocyanate
  • HSCN
  • Hydrogen thiocyanate
302-04-5
thiosulfate
  • Hyposulfite
  • S-Hydril
  • Sodium hyposulfite
  • Sodium oxide sulfide (Na2S2O3)
  • Sodium thiosulfate
14383-50-7
3-Mercaptolactate-cysteine disulfide
  • 3-((2-Carboxy-2-hydroxyethyl)dithio)-L-Alanine
  • beta-Mercaptolactate cysteine disulfide
  • Hcetc
  • S-(2-Hydroxy-2-carboxyethylthio)cysteine
18841-42-4
3-Mercaptopyruvic acid
  • 3-Mercapto-pyruvate
  • 3-Mercapto-pyruvic acid
  • 3-Mercaptopyruvate
  • 3-Mercaptopyruvic acid
  • beta-3-Mercapto-2-oxo-propanoate
2464-23-5
genes like me logo Genes that share compounds with MPST: view

Transcripts for MPST Gene

Unigene Clusters for MPST Gene

Mercaptopyruvate sulfurtransferase:
Representative Sequences:

Inhibitory RNA Products

Alternative Splicing Database (ASD) splice patterns (SP) for MPST Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b · 8c · 8d · 8e
SP1: -
SP2: - - - -
SP3: - - -
SP4:
SP5: -
SP6:
SP7: - - -

Relevant External Links for MPST Gene

GeneLoc Exon Structure for
MPST
ECgene alternative splicing isoforms for
MPST

Expression for MPST Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for MPST Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for MPST Gene

This gene is overexpressed in Liver (x7.1).

Protein differential expression in normal tissues from HIPED for MPST Gene

This gene is overexpressed in Liver, secretome (16.3) and Liver (9.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for MPST Gene



Protein tissue co-expression partners for MPST Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of MPST Gene:

MPST

SOURCE GeneReport for Unigene cluster for MPST Gene:

Hs.248267

Evidence on tissue expression from TISSUES for MPST Gene

  • Kidney(4.5)
  • Liver(4.5)
  • Nervous system(4.5)
  • Muscle(4.3)
  • Pancreas(4.3)
  • Bone marrow(4.2)
  • Blood(2)
genes like me logo Genes that share expression patterns with MPST: view

Primer Products

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for MPST Gene

Orthologs for MPST Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for MPST Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MPST 34 35
  • 98.84 (n)
dog
(Canis familiaris)
Mammalia MPST 34 35
  • 89.9 (n)
cow
(Bos Taurus)
Mammalia MPST 34 35
  • 86.46 (n)
mouse
(Mus musculus)
Mammalia Mpst 34 16 35
  • 83.67 (n)
rat
(Rattus norvegicus)
Mammalia Mpst 34
  • 82.43 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia MPST 35
  • 80 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia MPST 35
  • 79 (a)
OneToOne
chicken
(Gallus gallus)
Aves MPST 34 35
  • 70.61 (n)
lizard
(Anolis carolinensis)
Reptilia MPST 35
  • 75 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia mpst 34
  • 63.96 (n)
Str.1400 34
African clawed frog
(Xenopus laevis)
Amphibia Xl.3678 34
zebrafish
(Danio rerio)
Actinopterygii zgc:162544 34
  • 58.68 (n)
MPST 35
  • 56 (a)
OneToMany
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.2809 34
worm
(Caenorhabditis elegans)
Secernentea mpst-5 35
  • 26 (a)
ManyToMany
mpst-6 35
  • 26 (a)
ManyToMany
mpst-2 35
  • 23 (a)
ManyToMany
mpst-4 35
  • 23 (a)
ManyToMany
mpst-3 35
  • 22 (a)
ManyToMany
mpst-1 35
  • 21 (a)
ManyToMany
mpst-7 35
  • 18 (a)
ManyToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes TUM1 34 35 37
  • 46.04 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_AAL096W 34
  • 45.48 (n)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0C09966g 34
  • 44.44 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons MST1 34
  • 48.23 (n)
rice
(Oryza sativa)
Liliopsida Os12g0608600 34
  • 48.11 (n)
bread mold
(Neurospora crassa)
Ascomycetes NCU06017 34
  • 49.72 (n)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes tum1 34
  • 45.31 (n)
Species where no ortholog for MPST was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for MPST Gene

ENSEMBL:
Gene Tree for MPST (if available)
TreeFam:
Gene Tree for MPST (if available)

Paralogs for MPST Gene

Paralogs for MPST Gene

(1) SIMAP similar genes for MPST Gene using alignment to 6 proteins:

genes like me logo Genes that share paralogs with MPST: view

Variants for MPST Gene

Sequence variations from dbSNP and Humsavar for MPST Gene

SNP ID Clin Chr 22 pos Sequence Context AA Info Type
rs1000403867 -- 37,019,800(+) ACAGC(C/T)GCGGG intron-variant, upstream-variant-2KB, utr-variant-5-prime
rs1000490589 -- 37,021,533(+) AGTGG(C/T)GCGAT intron-variant, upstream-variant-2KB
rs1000606089 -- 37,021,181(+) GGCGG(C/T)TACGT intron-variant, upstream-variant-2KB
rs1000750792 -- 37,027,077(+) CCTCC(C/T)GAGTA intron-variant, utr-variant-3-prime
rs1001105855 -- 37,027,018(+) CGCAA(C/T)CTCGG intron-variant, reference, synonymous-codon

Structural Variations from Database of Genomic Variants (DGV) for MPST Gene

Variant ID Type Subtype PubMed ID
esv2661268 CNV deletion 23128226
esv34015 CNV loss 18971310
esv3558139 CNV deletion 23714750
esv3647699 CNV loss 21293372
nsv1057409 CNV gain 25217958
nsv3622 CNV insertion 18451855
nsv459882 CNV gain 19166990
nsv588984 CNV gain 21841781
nsv829203 CNV loss 20364138
nsv834187 CNV loss 17160897

Variation tolerance for MPST Gene

Residual Variation Intolerance Score: 45.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.80; 33.89% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for MPST Gene

Human Gene Mutation Database (HGMD)
MPST
SNPedia medical, phenotypic, and genealogical associations of SNPs for
MPST

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MPST Gene

Disorders for MPST Gene

MalaCards: The human disease database

(3) MalaCards diseases for MPST Gene - From: GeneCards

Disorder Aliases PubMed IDs
mercaptolactate-cysteine disulfiduria
  • ampola syndrome
cyanide poisoning
aminoaciduria
- elite association - COSMIC cancer census association via MalaCards
Search MPST in MalaCards View complete list of genes associated with diseases

UniProtKB/Swiss-Prot

THTM_HUMAN
  • Note=Aberrant MPST activity is found in a few cases of mercaptolactate-cysteine disulfiduria (MCDU) characterized by the appearance of large quantaties of the sulfur-containing amino acid, beta-mercaptolactate-cysteine disulfide, in the urine (PubMed:4973015, PubMed:4690911 and PubMed:6945862). Some cases have associated mental retardation (PubMed:4973015 and PubMed:6945862).

Relevant External Links for MPST

Genetic Association Database (GAD)
MPST
Human Genome Epidemiology (HuGE) Navigator
MPST
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
MPST
genes like me logo Genes that share disorders with MPST: view

No data available for Genatlas for MPST Gene

Publications for MPST Gene

  1. Cloning and sequence analysis of the human liver rhodanese: comparison with the bovine and chicken enzymes. (PMID: 1953758) Pallini R. … Cacace M.G. (Biochem. Biophys. Res. Commun. 1991) 2 3 4 64
  2. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. (PMID: 20877624) Hendrickson S.L. … O'Brien S.J. (PLoS ONE 2010) 3 46 64
  3. Evidence for a functional genetic polymorphism of the human mercaptopyruvate sulfurtransferase (MPST), a cyanide detoxification enzyme. (PMID: 16545926) Billaut-Laden I. … Broly F. (Toxicol. Lett. 2006) 3 22 64
  4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4 64
  5. A genome annotation-driven approach to cloning the human ORFeome. (PMID: 15461802) Collins J.E. … Dunham I. (Genome Biol. 2004) 3 4 64

Products for MPST Gene

Sources for MPST Gene

Content
Loading form....