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Aliases for MPST Gene

Aliases for MPST Gene

  • Mercaptopyruvate Sulfurtransferase 2 3
  • TRNA Thiouridin Modification Protein 1 2 3
  • Human Liver Rhodanese 2 3
  • TST2 3 4
  • MST 3 4
  • 3-Mercaptopyruvate Sulfurtransferase 3
  • EC 2.8.1.2 4
  • TUM1 3

External Ids for MPST Gene

Previous GeneCards Identifiers for MPST Gene

  • GC22P034030
  • GC22P035658
  • GC22P035740
  • GC22P037415
  • GC22P020380

Summaries for MPST Gene

Entrez Gene Summary for MPST Gene

  • This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

GeneCards Summary for MPST Gene

MPST (Mercaptopyruvate Sulfurtransferase) is a Protein Coding gene. Diseases associated with MPST include aminoaciduria. Among its related pathways are Metabolism and Sulfur relay system. GO annotations related to this gene include thiosulfate sulfurtransferase activity and 3-mercaptopyruvate sulfurtransferase activity. An important paralog of this gene is TST.

UniProtKB/Swiss-Prot for MPST Gene

  • Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions (By similarity).

Gene Wiki entry for MPST Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MPST Gene

Genomics for MPST Gene

Regulatory Elements for MPST Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for MPST Gene

Start:
37,019,635 bp from pter
End:
37,029,822 bp from pter
Size:
10,188 bases
Orientation:
Plus strand

Genomic View for MPST Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for MPST Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MPST Gene

Proteins for MPST Gene

  • Protein details for MPST Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P25325-THTM_HUMAN
    Recommended name:
    3-mercaptopyruvate sulfurtransferase
    Protein Accession:
    P25325
    Secondary Accessions:
    • A8MZ34
    • B3KP52
    • J3KPV7
    • O75750
    • Q6FHN9

    Protein attributes for MPST Gene

    Size:
    297 amino acids
    Molecular mass:
    33178 Da
    Quaternary structure:
    • Monomer; active form. Homodimer; disulfide-linked, inactive form.
    Miscellaneous:
    • Thioredoxin (Trx) or dihydrolipoic acid (DHLA) are required to release hydrogen sulfide from the persulfide intermediate.
    SequenceCaution:
    • Sequence=AAH16737.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=CAG30409.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for MPST Gene

    Alternative splice isoforms for MPST Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MPST Gene

Proteomics data for MPST Gene at MOPED

Post-translational modifications for MPST Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for MPST Gene

Domains for MPST Gene

Protein Domains for MPST Gene

Graphical View of Domain Structure for InterPro Entry

P25325

UniProtKB/Swiss-Prot:

THTM_HUMAN :
  • P25325
Domain:
  • Contains two rhodanese domains with different primary structures but with near identical secondary structure conformations suggesting a common evolutionary origin. Only the C-terminal rhodanese domain contains the catalytic cysteine residue (By similarity).
  • Contains 2 rhodanese domains.
genes like me logo Genes that share domains with MPST: view

Function for MPST Gene

Molecular function for MPST Gene

GENATLAS Biochemistry: mercaptopyruvate sulfurtransferase
UniProtKB/Swiss-Prot CatalyticActivity: 3-mercaptopyruvate + cyanide = pyruvate + thiocyanate
UniProtKB/Swiss-Prot EnzymeRegulation: By oxidative stress, and thioredoxin. Under oxidative stress conditions, the catalytic cysteine site is converted to a sulfenate which inhibits the MPST enzyme activity. Reduced thioredoxin cleaves an intersubunit disulfide bond to turn on the redox switch and reactivate the enzyme
UniProtKB/Swiss-Prot Function: Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions (By similarity).

Enzyme Numbers (IUBMB) for MPST Gene

Gene Ontology (GO) - Molecular Function for MPST Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004792 thiosulfate sulfurtransferase activity IEA --
GO:0016784 3-mercaptopyruvate sulfurtransferase activity IEA --
genes like me logo Genes that share ontologies with MPST: view

Animal Model Products

CRISPR Products

miRNA for MPST Gene

miRTarBase miRNAs that target MPST

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for MPST

In Situ Assay Products

Flow Cytometry Products

No data available for Phenotypes , Animal Models , Transcription Factor Targeting and HOMER Transcription for MPST Gene

Localization for MPST Gene

Subcellular locations from UniProtKB/Swiss-Prot for MPST Gene

Cytoplasm. Mitochondrion. Cell junction, synapse, synaptosome.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for MPST Gene COMPARTMENTS Subcellular localization image for MPST gene
Compartment Confidence
mitochondrion 4
cytosol 3
nucleus 2
extracellular 1

Gene Ontology (GO) - Cellular Components for MPST Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm --
GO:0005739 mitochondrion --
GO:0005743 mitochondrial inner membrane IEA --
GO:0030054 cell junction IEA --
GO:0043005 neuron projection ISS --
genes like me logo Genes that share ontologies with MPST: view

Pathways for MPST Gene

genes like me logo Genes that share pathways with MPST: view

Pathways by source for MPST Gene

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Interacting Proteins for MPST Gene

Selected Interacting proteins: P25325-THTM_HUMAN for MPST Gene via I2D

Gene Ontology (GO) - Biological Process for MPST Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0008152 metabolic process --
GO:0009440 cyanate catabolic process TAS 9070219
GO:0009636 response to toxic substance TAS 9070219
GO:0070814 hydrogen sulfide biosynthetic process ISS --
genes like me logo Genes that share ontologies with MPST: view

Compounds for MPST Gene

(8) HMDB Compounds for MPST Gene

Compound Synonyms Cas Number PubMed IDs
3-Mercaptolactate-cysteine disulfide
  • 3-((2-carboxy-2-hydroxyethyl)dithio)-L-Alanine
18841-42-4
3-Mercaptopyruvic acid
  • 3-Mercaptopyruvate
2464-23-5
Cyanide
  • Carbon nitride ion
57-12-5
Ethanol
  • 1-Hydroxyethane
64-17-5
Pyruvic acid
  • 2-Oxopropanoate
127-17-3

(7) Novoseek inferred chemical compound relationships for MPST Gene

Compound -log(P) Hits PubMed IDs
3-mercaptopyruvate 98.9 31
thiosulfate 75.6 2
thiocyanate 67.3 2
cyanide 67.3 11
sulfur 49.5 3
genes like me logo Genes that share compounds with MPST: view

Transcripts for MPST Gene

Unigene Clusters for MPST Gene

Mercaptopyruvate sulfurtransferase:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for MPST

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for MPST Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b · 8c · 8d · 8e
SP1: -
SP2: - - - -
SP3: - - -
SP4:
SP5: -
SP6:
SP7: - - -

Relevant External Links for MPST Gene

GeneLoc Exon Structure for
MPST
ECgene alternative splicing isoforms for
MPST

Expression for MPST Gene

mRNA expression in normal human tissues for MPST Gene

mRNA differential expression in normal tissues according to GTEx for MPST Gene

This gene is overexpressed in Liver (7.1).

Integrated Proteomics: protein expression from ProteomicsDB, MOPED, and MaxQB for MPST Gene

SOURCE GeneReport for Unigene cluster for MPST Gene Hs.248267

genes like me logo Genes that share expressions with MPST: view

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA Expression by UniProt/SwissProt for MPST Gene

Orthologs for MPST Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for MPST Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MPST 35
  • 98.84 (n)
  • 98.74 (a)
MPST 36
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia MPST 35
  • 86.46 (n)
  • 88.89 (a)
MPST 36
  • 89 (a)
OneToOne
dog
(Canis familiaris)
Mammalia MPST 35
  • 89.9 (n)
  • 90.91 (a)
MPST 36
  • 91 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Mpst 35
  • 83.67 (n)
  • 84.8 (a)
Mpst 16
Mpst 36
  • 84 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia MPST 36
  • 79 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia MPST 36
  • 80 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Mpst 35
  • 82.43 (n)
  • 85.14 (a)
chicken
(Gallus gallus)
Aves MPST 35
  • 70.61 (n)
  • 73.65 (a)
MPST 36
  • 67 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia MPST 36
  • 75 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.3678 35
tropical clawed frog
(Silurana tropicalis)
Amphibia mpst 35
  • 63.96 (n)
  • 66.55 (a)
Str.1400 35
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.2809 35
zebrafish
(Danio rerio)
Actinopterygii MPST 36
  • 56 (a)
OneToMany
zgc:162544 35
  • 58.68 (n)
  • 58.68 (a)
worm
(Caenorhabditis elegans)
Secernentea mpst-1 36
  • 21 (a)
ManyToMany
mpst-2 36
  • 23 (a)
ManyToMany
mpst-3 36
  • 22 (a)
ManyToMany
mpst-4 36
  • 23 (a)
ManyToMany
mpst-5 36
  • 26 (a)
ManyToMany
mpst-6 36
  • 26 (a)
ManyToMany
mpst-7 36
  • 18 (a)
ManyToMany
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_AAL096W 35
  • 45.48 (n)
  • 36.43 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes TUM1 35
  • 46.04 (n)
  • 40.47 (a)
TUM1 36
  • 24 (a)
OneToMany
TUM1 38
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0C09966g 35
  • 44.44 (n)
  • 37.4 (a)
thale cress
(Arabidopsis thaliana)
eudicotyledons MST1 35
  • 48.23 (n)
  • 45.45 (a)
rice
(Oryza sativa)
Liliopsida Os12g0608600 35
  • 48.11 (n)
  • 45.39 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU06017 35
  • 49.72 (n)
  • 39.39 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes tum1 35
  • 45.31 (n)
  • 40.62 (a)
Species with no ortholog for MPST:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for MPST Gene

ENSEMBL:
Gene Tree for MPST (if available)
TreeFam:
Gene Tree for MPST (if available)

Paralogs for MPST Gene

Paralogs for MPST Gene

Selected SIMAP similar genes for MPST Gene using alignment to 6 proteins:

genes like me logo Genes that share paralogs with MPST: view

Variants for MPST Gene

Sequence variations from dbSNP and Humsavar for MPST Gene

SNP ID Clin Chr 22 pos Sequence Context AA Info Type MAF
rs1049282 -- 37,029,732(+) ACAAC(A/T)CCGAG utr-variant-3-prime
rs2187723 -- 37,026,349(+) GCACT(C/G)AGCTC intron-variant
rs3753143 -- 37,027,101(+) GCATG(C/T)GCCAC intron-variant, utr-variant-3-prime
rs3753144 -- 37,027,444(+) TCTAG(A/G)TTGGG intron-variant, utr-variant-3-prime
rs3753145 -- 37,027,445(+) CTAGG(G/T)TGGGC intron-variant, utr-variant-3-prime

Structural Variations from Database of Genomic Variants (DGV) for MPST Gene

Variant ID Type Subtype PubMed ID
nsv834187 CNV Loss 17160897
esv34015 CNV Loss 18971310
nsv459882 CNV Gain 19166990
nsv829203 CNV Loss 20364138
dgv4960n71 CNV Loss 21882294
nsv3622 CNV Insertion 18451855
esv2661268 CNV Deletion 23128226

Relevant External Links for MPST Gene

HapMap Linkage Disequilibrium report
MPST
Human Gene Mutation Database (HGMD)
MPST

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MPST Gene

Disorders for MPST Gene

MalaCards: The human disease database

MalaCards: The human disease database. (1) Diseases for MPST Gene including...

Search for MPST Gene in MalaCards »

UniProtKB/Swiss-Prot

THTM_HUMAN
  • Note=Aberrant MPST activity is found in a few cases of mercaptolactate-cysteine disulfiduria (MCDU) characterized by the appearance of large quantaties of the sulfur-containing amino acid, beta-mercaptolactate-cysteine disulfide, in the urine (PubMed:4973015, PubMed:4690911 and PubMed:6945862). Some cases have associated mental retardation (PubMed:4973015 and PubMed:6945862).

Relevant External Links for MPST

Genetic Association Database (GAD)
MPST
Human Genome Epidemiology (HuGE) Navigator
MPST
genes like me logo Genes that share disorders with MPST: view

Publications for MPST Gene

  1. Cloning and sequence analysis of the human liver rhodanese: comparison with the bovine and chicken enzymes. (PMID: 1953758) Pallini R. … Cacace M.G. (Biochem. Biophys. Res. Commun. 1991) 2 3 4
  2. The DNA sequence of human chromosome 22. (PMID: 10591208) Dunham I. … Wright H. (Nature 1999) 3 4
  3. Evidence for a functional genetic polymorphism of the human mercaptopyruvate sulfurtransferase (MPST), a cyanide detoxification enzyme. (PMID: 16545926) Billaut-Laden I. … Broly F. (Toxicol. Lett. 2006) 3 23
  4. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. (PMID: 20877624) Hendrickson S.L. … O'Brien S.J. (PLoS ONE 2010) 3 48
  5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4

Products for MPST Gene

Sources for MPST Gene

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