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Aliases for MPHOSPH8 Gene

Aliases for MPHOSPH8 Gene

  • M-Phase Phosphoprotein 8 2 3 5
  • Two Hybrid-Associated Protein 3 With RanBPM 3 4
  • TWA3 3 4
  • Mpp8 3 4
  • M-Phase Phosphoprotein, Mpp8 3
  • M-Phase Phosphoprotein, Mpp 3
  • HSMPP8 3

External Ids for MPHOSPH8 Gene

Previous GeneCards Identifiers for MPHOSPH8 Gene

  • GC13P019107
  • GC13P020208
  • GC13P001012

Summaries for MPHOSPH8 Gene

GeneCards Summary for MPHOSPH8 Gene

MPHOSPH8 (M-Phase Phosphoprotein 8) is a Protein Coding gene. GO annotations related to this gene include methylated histone binding.

UniProtKB/Swiss-Prot for MPHOSPH8 Gene

  • Heterochromatin component that specifically recognizes and binds methylated Lys-9 of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3 (PubMed:26022416). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592).

Gene Wiki entry for MPHOSPH8 Gene

No data available for Entrez Gene Summary , CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MPHOSPH8 Gene

Genomics for MPHOSPH8 Gene

Regulatory Elements for MPHOSPH8 Gene

Enhancers for MPHOSPH8 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH13G019601 1.2 Ensembl ENCODE 29.2 -31.4 -31435 1.6 CREB3L1 SIN3A DMAP1 ELK1 ZNF143 ZNF207 KAT8 ZNF585B MXD3 SP7 MPHOSPH8 ESRRAP1 PSPC1-AS2 PSPC1 ZMYM5 PIR42308 LINC00350
GH13G019632 1.1 ENCODE 17.7 +0.4 376 2.6 HDGF PKNOX1 AGO1 WRNIP1 ARID4B SIN3A YBX1 ZNF2 YY1 SLC30A9 MPHOSPH8 PSPC1-AS2 PSPC1 ZMYM5 LOC101928697 GC13M019667
GH13G020126 1.6 FANTOM5 Ensembl ENCODE 11.3 +494.1 494128 2.5 PKNOX1 ARID4B SIN3A DMAP1 GLI4 ZNF2 ZNF48 YY1 ZNF121 GLIS2 GJA3 PPIAP28 PSPC1-AS2 MPHOSPH8 CRYL1 PARP4P2 ZMYM5 PSPC1 LOC105370101
GH13G019845 1.5 FANTOM5 Ensembl ENCODE 11.6 +212.4 212362 1.8 ATF1 SIN3A FEZF1 CBX5 FOS JUNB TSHZ1 TBX21 MAFF GLIS1 PSPC1 MPHOSPH8 ZMYM5 ENSG00000223576
GH13G019793 1.1 Ensembl ENCODE 14.8 +161.2 161186 3.1 PKNOX1 SIN3A RAD21 CBX5 ETV6 FOS RCOR1 RELB CREM IKZF2 MPHOSPH8 ENSG00000223576 PSPC1-AS2 PSPC1 ST6GALNAC4P1
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around MPHOSPH8 on UCSC Golden Path with GeneCards custom track

Promoters for MPHOSPH8 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000059611 354 2400 HDGF PKNOX1 AGO1 WRNIP1 ARID4B SIN3A YBX1 ZNF2 YY1 SLC30A9

Genomic Location for MPHOSPH8 Gene

Chromosome:
13
Start:
19,633,647 bp from pter
End:
19,673,459 bp from pter
Size:
39,813 bases
Orientation:
Plus strand

Genomic View for MPHOSPH8 Gene

Genes around MPHOSPH8 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MPHOSPH8 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MPHOSPH8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MPHOSPH8 Gene

Proteins for MPHOSPH8 Gene

  • Protein details for MPHOSPH8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q99549-MPP8_HUMAN
    Recommended name:
    M-phase phosphoprotein 8
    Protein Accession:
    Q99549
    Secondary Accessions:
    • B7Z6F9
    • Q5JPE5
    • Q5JTQ0
    • Q86TK3
    • Q96MK4
    • Q9BTP1

    Protein attributes for MPHOSPH8 Gene

    Size:
    860 amino acids
    Molecular mass:
    97182 Da
    Quaternary structure:
    • Homodimer (PubMed:21419134, PubMed:22022377, PubMed:22086334). Interacts (via chromo domain) with histone H3K9me3 (PubMed:20871592). Has the highest affinity for H3K9me3, and lesser affinity for H3K9me2 and H3K9me1 (PubMed:20871592). Component of the HUSH complex; at least composed of FAM208A/TASOR, PPHLN1 and MPHOSPH8 (PubMed:26022416). Interacts with DNMT3, EHMT1 and SETDB1 (PubMed:20871592, PubMed:22086334).
    SequenceCaution:
    • Sequence=AAH46214.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAB71284.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=CAI46172.1; Type=Frameshift; Positions=248, 255, 731; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for MPHOSPH8 Gene

    Alternative splice isoforms for MPHOSPH8 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MPHOSPH8 Gene

Post-translational modifications for MPHOSPH8 Gene

  • Phosphorylated in M (mitotic) phase. Phosphorylation by CDK1 promotes dissociation from chromatin.
  • Ubiquitination at posLast=708708
  • Modification sites at PhosphoSitePlus

Other Protein References for MPHOSPH8 Gene

No data available for DME Specific Peptides for MPHOSPH8 Gene

Domains & Families for MPHOSPH8 Gene

Gene Families for MPHOSPH8 Gene

Suggested Antigen Peptide Sequences for MPHOSPH8 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q99549

UniProtKB/Swiss-Prot:

MPP8_HUMAN :
  • The chromo domain mediates interaction with methylated Lys-9 of histone H3 (H3K9me), with the highest affinity for the trimethylated form (H3K9me3).
Domain:
  • The chromo domain mediates interaction with methylated Lys-9 of histone H3 (H3K9me), with the highest affinity for the trimethylated form (H3K9me3).
genes like me logo Genes that share domains with MPHOSPH8: view

Function for MPHOSPH8 Gene

Molecular function for MPHOSPH8 Gene

UniProtKB/Swiss-Prot Function:
Heterochromatin component that specifically recognizes and binds methylated Lys-9 of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3 (PubMed:26022416). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592).

Gene Ontology (GO) - Molecular Function for MPHOSPH8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004842 ubiquitin-protein transferase activity IBA --
GO:0005515 protein binding IPI 20871592
GO:0031625 ubiquitin protein ligase binding IBA --
GO:0035064 methylated histone binding IDA 20871592
genes like me logo Genes that share ontologies with MPHOSPH8: view
genes like me logo Genes that share phenotypes with MPHOSPH8: view

Animal Model Products

CRISPR Products

Inhibitory RNA Products

Clone Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for MPHOSPH8 Gene

Localization for MPHOSPH8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MPHOSPH8 Gene

Nucleus. Chromosome. Note=Detected on heterochromatin (PubMed:20871592, PubMed:26022416). Dissociates from chromatin during interphase and early mitosis (PubMed:23416073). Detected on nucleosomes (PubMed:20871592). {ECO:0000269 PubMed:20871592, ECO:0000269 PubMed:23416073, ECO:0000269 PubMed:26022416}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MPHOSPH8 gene
Compartment Confidence
plasma membrane 5
extracellular 5
nucleus 5
cytosol 5

Gene Ontology (GO) - Cellular Components for MPHOSPH8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000151 ubiquitin ligase complex IBA --
GO:0000788 nuclear nucleosome IDA 20871592
GO:0005634 nucleus IDA 8885239
GO:0005694 chromosome IEA --
GO:0005720 nuclear heterochromatin IDA 20871592
genes like me logo Genes that share ontologies with MPHOSPH8: view

Pathways & Interactions for MPHOSPH8 Gene

SuperPathways for MPHOSPH8 Gene

No Data Available

Gene Ontology (GO) - Biological Process for MPHOSPH8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006351 transcription, DNA-templated IEA --
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0016567 protein ubiquitination IEA --
GO:0044030 regulation of DNA methylation IMP 20871592
GO:0045892 negative regulation of transcription, DNA-templated IMP 20871592
genes like me logo Genes that share ontologies with MPHOSPH8: view

No data available for Pathways by source and SIGNOR curated interactions for MPHOSPH8 Gene

Drugs & Compounds for MPHOSPH8 Gene

No Compound Related Data Available

Transcripts for MPHOSPH8 Gene

Unigene Clusters for MPHOSPH8 Gene

M-phase phosphoprotein 8:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for MPHOSPH8 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^ 14 ^ 15a · 15b
SP1: -
SP2:
SP3:
SP4:
SP5:

Relevant External Links for MPHOSPH8 Gene

GeneLoc Exon Structure for
MPHOSPH8
ECgene alternative splicing isoforms for
MPHOSPH8

Expression for MPHOSPH8 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for MPHOSPH8 Gene

Protein differential expression in normal tissues from HIPED for MPHOSPH8 Gene

This gene is overexpressed in Testis (14.0), Peripheral blood mononuclear cells (12.1), Heart (7.9), and Pancreas (7.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for MPHOSPH8 Gene



Protein tissue co-expression partners for MPHOSPH8 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of MPHOSPH8 Gene:

MPHOSPH8

SOURCE GeneReport for Unigene cluster for MPHOSPH8 Gene:

Hs.269654

Evidence on tissue expression from TISSUES for MPHOSPH8 Gene

  • Nervous system(4.6)
  • Liver(4.3)
  • Lymph node(4.3)
  • Pancreas(4.3)
  • Blood(4.2)
  • Intestine(3.6)
  • Spleen(2.1)
genes like me logo Genes that share expression patterns with MPHOSPH8: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for MPHOSPH8 Gene

Orthologs for MPHOSPH8 Gene

This gene was present in the common ancestor of chordates.

Orthologs for MPHOSPH8 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MPHOSPH8 34 35
  • 99.38 (n)
dog
(Canis familiaris)
Mammalia MPHOSPH8 34
  • 86.44 (n)
cow
(Bos Taurus)
Mammalia MPHOSPH8 34 35
  • 82.86 (n)
rat
(Rattus norvegicus)
Mammalia Mphosph8 34
  • 80.87 (n)
mouse
(Mus musculus)
Mammalia Mphosph8 34 16 35
  • 80.55 (n)
oppossum
(Monodelphis domestica)
Mammalia MPHOSPH8 35
  • 74 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia MPHOSPH8 35
  • 74 (a)
OneToOne
chicken
(Gallus gallus)
Aves MPHOSPH8 34 35
  • 68.84 (n)
lizard
(Anolis carolinensis)
Reptilia MPHOSPH8 35
  • 54 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia mphosph8 34
  • 64.92 (n)
Str.2500 34
zebrafish
(Danio rerio)
Actinopterygii mphosph8 35
  • 41 (a)
OneToOne
wufc38d04 34
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.4384 34
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 32 (a)
OneToMany
-- 35
  • 31 (a)
OneToMany
Species where no ortholog for MPHOSPH8 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MPHOSPH8 Gene

ENSEMBL:
Gene Tree for MPHOSPH8 (if available)
TreeFam:
Gene Tree for MPHOSPH8 (if available)

Paralogs for MPHOSPH8 Gene

(3) SIMAP similar genes for MPHOSPH8 Gene using alignment to 2 proteins:

Pseudogenes.org Pseudogenes for MPHOSPH8 Gene

genes like me logo Genes that share paralogs with MPHOSPH8: view

No data available for Paralogs for MPHOSPH8 Gene

Variants for MPHOSPH8 Gene

Sequence variations from dbSNP and Humsavar for MPHOSPH8 Gene

SNP ID Clin Chr 13 pos Sequence Context AA Info Type
rs1000077422 -- 19,672,533(+) AGCTC(C/T)AAAAC nc-transcript-variant, utr-variant-3-prime
rs1000197650 -- 19,646,752(+) AAGAA(C/T)TAAAG nc-transcript-variant, reference, synonymous-codon
rs1000457086 -- 19,649,917(+) CTTGT(C/T)GCTTT intron-variant
rs1000509589 -- 19,656,373(+) TAGCA(A/C)GGTGA intron-variant
rs1000580413 -- 19,655,045(+) GTTTT(A/T)AAAAA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for MPHOSPH8 Gene

Variant ID Type Subtype PubMed ID
dgv1607n100 CNV gain 25217958
dgv1608n100 CNV gain 25217958
dgv334e199 CNV deletion 23128226
esv2747051 CNV deletion 23290073
esv2761244 CNV gain 21179565
esv3582126 CNV gain 25503493
esv3582138 CNV gain 25503493
esv3631393 CNV gain 21293372
esv3631396 CNV gain 21293372
esv3631398 CNV gain 21293372
esv3892298 CNV gain 25118596
esv3892299 CNV gain 25118596
nsv1041042 CNV gain 25217958
nsv1047555 CNV loss 25217958
nsv1051666 CNV gain 25217958
nsv1054226 CNV gain 25217958
nsv1078303 OTHER inversion 25765185
nsv1115628 CNV deletion 24896259
nsv1140246 OTHER inversion 24896259
nsv1148310 CNV deletion 26484159
nsv1151909 OTHER inversion 26484159
nsv516945 CNV gain+loss 19592680
nsv977201 CNV duplication 23825009

Variation tolerance for MPHOSPH8 Gene

Residual Variation Intolerance Score: 22.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.69; 57.28% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for MPHOSPH8 Gene

Human Gene Mutation Database (HGMD)
MPHOSPH8
SNPedia medical, phenotypic, and genealogical associations of SNPs for
MPHOSPH8

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MPHOSPH8 Gene

Disorders for MPHOSPH8 Gene

Relevant External Links for MPHOSPH8

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
MPHOSPH8

No disorders were found for MPHOSPH8 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for MPHOSPH8 Gene

Publications for MPHOSPH8 Gene

  1. Identification of novel M phase phosphoproteins by expression cloning. (PMID: 8885239) Matsumoto-Taniura N. … Westendorf J.M. (Mol. Biol. Cell 1996) 2 3 4 64
  2. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells. (PMID: 26022416) Tchasovnikarova I.A. … Lehner P.J. (Science 2015) 3 4 64
  3. Mitotic phosphorylation of MPP8 by cyclin-dependent kinases regulates chromatin dissociation. (PMID: 23416073) Nishigaki M. … Nakanishi M. (Biochem. Biophys. Res. Commun. 2013) 3 4 64
  4. Structural insights for MPP8 chromodomain interaction with histone H3 lysine 9: potential effect of phosphorylation on methyl-lysine binding. (PMID: 21419134) Chang Y. … Cheng X. (J. Mol. Biol. 2011) 3 4 64
  5. Structural basis for specific binding of human MPP8 chromodomain to histone H3 methylated at lysine 9. (PMID: 22022377) Li J. … Zang J. (PLoS ONE 2011) 3 4 64

Products for MPHOSPH8 Gene

Sources for MPHOSPH8 Gene

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