Free for academic non-profit institutions. Other users need a Commercial license

Aliases for MPHOSPH8 Gene

Aliases for MPHOSPH8 Gene

  • M-Phase Phosphoprotein 8 2 3 5
  • Two Hybrid-Associated Protein 3 With RanBPM 3 4
  • TWA3 3 4
  • Mpp8 3 4
  • M-Phase Phosphoprotein, Mpp8 3
  • M-Phase Phosphoprotein, Mpp 3
  • HSMPP8 3

External Ids for MPHOSPH8 Gene

Previous GeneCards Identifiers for MPHOSPH8 Gene

  • GC13P019107
  • GC13P020208
  • GC13P001012

Summaries for MPHOSPH8 Gene

GeneCards Summary for MPHOSPH8 Gene

MPHOSPH8 (M-Phase Phosphoprotein 8) is a Protein Coding gene. GO annotations related to this gene include methylated histone binding.

UniProtKB/Swiss-Prot for MPHOSPH8 Gene

  • Heterochromatin component that specifically recognizes and binds methylated Lys-9 of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3 (PubMed:26022416). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592).

Gene Wiki entry for MPHOSPH8 Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MPHOSPH8 Gene

Genomics for MPHOSPH8 Gene

Regulatory Elements for MPHOSPH8 Gene

Promoters for MPHOSPH8 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around MPHOSPH8 on UCSC Golden Path with GeneCards custom track

Genomic Location for MPHOSPH8 Gene

Chromosome:
13
Start:
19,633,647 bp from pter
End:
19,673,459 bp from pter
Size:
39,813 bases
Orientation:
Plus strand

Genomic View for MPHOSPH8 Gene

Genes around MPHOSPH8 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MPHOSPH8 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MPHOSPH8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MPHOSPH8 Gene

Proteins for MPHOSPH8 Gene

  • Protein details for MPHOSPH8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q99549-MPP8_HUMAN
    Recommended name:
    M-phase phosphoprotein 8
    Protein Accession:
    Q99549
    Secondary Accessions:
    • B7Z6F9
    • Q5JPE5
    • Q5JTQ0
    • Q86TK3
    • Q96MK4
    • Q9BTP1

    Protein attributes for MPHOSPH8 Gene

    Size:
    860 amino acids
    Molecular mass:
    97182 Da
    Quaternary structure:
    • Homodimer (PubMed:21419134, PubMed:22022377, PubMed:22086334). Interacts (via chromo domain) with histone H3K9me3 (PubMed:20871592). Has the highest affinity for H3K9me3, and lesser affinity for H3K9me2 and H3K9me1 (PubMed:20871592). Component of the HUSH complex; at least composed of FAM208A/TASOR, PPHLN1 and MPHOSPH8 (PubMed:26022416). Interacts with DNMT3, EHMT1 and SETDB1 (PubMed:20871592, PubMed:22086334).
    SequenceCaution:
    • Sequence=AAH46214.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAB71284.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=CAI46172.1; Type=Frameshift; Positions=248, 255, 731; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for MPHOSPH8 Gene

    Alternative splice isoforms for MPHOSPH8 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MPHOSPH8 Gene

Proteomics data for MPHOSPH8 Gene at MOPED

Post-translational modifications for MPHOSPH8 Gene

  • Phosphorylated in M (mitotic) phase. Phosphorylation by CDK1 promotes dissociation from chromatin.
  • Ubiquitination at Lys 708
  • Modification sites at PhosphoSitePlus

Other Protein References for MPHOSPH8 Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

No data available for DME Specific Peptides for MPHOSPH8 Gene

Domains & Families for MPHOSPH8 Gene

Gene Families for MPHOSPH8 Gene

Suggested Antigen Peptide Sequences for MPHOSPH8 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q99549

UniProtKB/Swiss-Prot:

MPP8_HUMAN :
  • The chromo domain mediates interaction with methylated Lys-9 of histone H3 (H3K9me), with the highest affinity for the trimethylated form (H3K9me3).
  • Contains 4 ANK repeats.
Domain:
  • The chromo domain mediates interaction with methylated Lys-9 of histone H3 (H3K9me), with the highest affinity for the trimethylated form (H3K9me3).
  • Contains 1 chromo domain.
Similarity:
  • Contains 4 ANK repeats.
genes like me logo Genes that share domains with MPHOSPH8: view

Function for MPHOSPH8 Gene

Molecular function for MPHOSPH8 Gene

UniProtKB/Swiss-Prot Function:
Heterochromatin component that specifically recognizes and binds methylated Lys-9 of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3 (PubMed:26022416). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592).
genes like me logo Genes that share phenotypes with MPHOSPH8: view

Animal Model Products

CRISPR Products

Inhibitory RNA Products

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Gene Ontology (GO) - Molecular Function , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for MPHOSPH8 Gene

Localization for MPHOSPH8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MPHOSPH8 Gene

Nucleus. Chromosome. Note=Detected on heterochromatin (PubMed:20871592, PubMed:26022416). Dissociates from chromatin during interphase and early mitosis (PubMed:23416073). Detected on nucleosomes (PubMed:20871592). {ECO:0000269 PubMed:20871592, ECO:0000269 PubMed:23416073, ECO:0000269 PubMed:26022416}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for MPHOSPH8 Gene COMPARTMENTS Subcellular localization image for MPHOSPH8 gene
Compartment Confidence
extracellular 5
nucleus 5
plasma membrane 5
cytosol 2

Gene Ontology (GO) - Cellular Components for MPHOSPH8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000788 nuclear nucleosome IDA 20871592
GO:0005730 nucleolus IDA --
GO:0005737 cytoplasm IDA 8885239
GO:0070062 extracellular exosome IDA 23376485
genes like me logo Genes that share ontologies with MPHOSPH8: view

Pathways & Interactions for MPHOSPH8 Gene

SuperPathways for MPHOSPH8 Gene

No Data Available

Gene Ontology (GO) - Biological Process for MPHOSPH8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0044030 regulation of DNA methylation IMP 20871592
genes like me logo Genes that share ontologies with MPHOSPH8: view

No data available for Pathways by source and SIGNOR curated interactions for MPHOSPH8 Gene

Drugs & Compounds for MPHOSPH8 Gene

No Compound Related Data Available

Transcripts for MPHOSPH8 Gene

Unigene Clusters for MPHOSPH8 Gene

M-phase phosphoprotein 8:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for MPHOSPH8 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^ 14 ^ 15a · 15b
SP1: -
SP2:
SP3:
SP4:
SP5:

Relevant External Links for MPHOSPH8 Gene

GeneLoc Exon Structure for
MPHOSPH8
ECgene alternative splicing isoforms for
MPHOSPH8

Expression for MPHOSPH8 Gene

mRNA expression in normal human tissues for MPHOSPH8 Gene

Protein differential expression in normal tissues from HIPED for MPHOSPH8 Gene

This gene is overexpressed in Testis (14.0), Peripheral blood mononuclear cells (12.1), Heart (7.9), and Pancreas (7.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for MPHOSPH8 Gene



SOURCE GeneReport for Unigene cluster for MPHOSPH8 Gene Hs.269654

genes like me logo Genes that share expression patterns with MPHOSPH8: view

Protein tissue co-expression partners for MPHOSPH8 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for MPHOSPH8 Gene

Orthologs for MPHOSPH8 Gene

This gene was present in the common ancestor of chordates.

Orthologs for MPHOSPH8 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia MPHOSPH8 35
  • 82.86 (n)
  • 81.35 (a)
MPHOSPH8 36
  • 80 (a)
OneToOne
dog
(Canis familiaris)
Mammalia MPHOSPH8 35
  • 86.44 (n)
  • 85.81 (a)
mouse
(Mus musculus)
Mammalia Mphosph8 35
  • 80.55 (n)
  • 78.82 (a)
Mphosph8 16
Mphosph8 36
  • 78 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia MPHOSPH8 35
  • 99.38 (n)
  • 99.42 (a)
MPHOSPH8 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Mphosph8 35
  • 80.87 (n)
  • 80.54 (a)
oppossum
(Monodelphis domestica)
Mammalia MPHOSPH8 36
  • 74 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia MPHOSPH8 36
  • 74 (a)
OneToOne
chicken
(Gallus gallus)
Aves MPHOSPH8 35
  • 68.84 (n)
  • 65.8 (a)
MPHOSPH8 36
  • 63 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia MPHOSPH8 36
  • 54 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia mphosph8 35
  • 64.92 (n)
  • 60.87 (a)
Str.2500 35
zebrafish
(Danio rerio)
Actinopterygii wufc38d04 35
mphosph8 36
  • 41 (a)
OneToOne
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.4384 35
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 31 (a)
OneToMany
-- 36
  • 32 (a)
OneToMany
Species with no ortholog for MPHOSPH8:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MPHOSPH8 Gene

ENSEMBL:
Gene Tree for MPHOSPH8 (if available)
TreeFam:
Gene Tree for MPHOSPH8 (if available)

Paralogs for MPHOSPH8 Gene

(3) SIMAP similar genes for MPHOSPH8 Gene using alignment to 2 proteins:

Pseudogenes.org Pseudogenes for MPHOSPH8 Gene

genes like me logo Genes that share paralogs with MPHOSPH8: view

No data available for Paralogs for MPHOSPH8 Gene

Variants for MPHOSPH8 Gene

Sequence variations from dbSNP and Humsavar for MPHOSPH8 Gene

SNP ID Clin Chr 13 pos Sequence Context AA Info Type
rs167117 -- 19,644,165(-) ATACA(A/C)ACAGA intron-variant
rs176796 -- 19,644,297(-) ATATA(G/T)AGGGA intron-variant
rs417674 -- 19,634,254(-) GCTCC(C/T)TTCAC intron-variant
rs456149 -- 19,640,097(+) tctta(A/T)aaaaa intron-variant
rs456229 -- 19,647,677(+) CATTA(A/C/G)CCAGT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for MPHOSPH8 Gene

Variant ID Type Subtype PubMed ID
esv2747051 CNV Deletion 23290073
dgv334e199 CNV Deletion 23128226
nsv899855 CNV Gain 21882294
nsv516945 CNV Gain+Loss 19592680
nsv899856 CNV Gain 21882294
nsv899857 CNV Gain 21882294

Variation tolerance for MPHOSPH8 Gene

Residual Variation Intolerance Score: 22.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.69; 57.28% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for MPHOSPH8 Gene

HapMap Linkage Disequilibrium report
MPHOSPH8
Human Gene Mutation Database (HGMD)
MPHOSPH8

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MPHOSPH8 Gene

Disorders for MPHOSPH8 Gene

Relevant External Links for MPHOSPH8

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
MPHOSPH8

No disorders were found for MPHOSPH8 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for MPHOSPH8 Gene

Publications for MPHOSPH8 Gene

  1. Identification of novel M phase phosphoproteins by expression cloning. (PMID: 8885239) Matsumoto-Taniura N. … Westendorf J.M. (Mol. Biol. Cell 1996) 2 3 4 67
  2. Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis. (PMID: 25660450) Murata K. … Shimada M. (Biochem. Biophys. Res. Commun. 2015) 3
  3. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells. (PMID: 26022416) Tchasovnikarova I.A. … Lehner P.J. (Science 2015) 3
  4. The BioPlex Network: A Systematic Exploration of the Human Interactome. (PMID: 26186194) Huttlin E.L. … Gygi S.P. (Cell 2015) 3
  5. The central role of EED in the orchestration of polycomb group complexes. (PMID: 24457600) Cao Q. … Chinnaiyan A.M. (Nat Commun 2014) 3

Products for MPHOSPH8 Gene

Sources for MPHOSPH8 Gene

Back to Top

Content