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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

MPHOSPH6 Gene

protein-coding   GIFtS: 53
GCID: GC16M082181

M-phase phosphoprotein 6

  Search for MPHOSPH6
in our new
 Human Malady Compendium 
Biological research products
for MPHOSPH6
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
M-Phase Phosphoprotein 61 2
MPP61 2 3
MPP2 5
MPP-62

External Ids:    HGNC: 72141   Entrez Gene: 102002   Ensembl: ENSG000001356987   OMIM: 6055005   UniProtKB: Q995473   

Export aliases for MPHOSPH6 gene to outside databases

Previous GC identifers: GC16M073023 GC16M083142 GC16M081920 GC16M081961 GC16M080739 GC16M067936


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: MPH6_HUMAN, Q99547
Function: RNA-binding protein that associates with the RNA exosome complex. Involved in the 3'processing of the 7S
pre-RNA to the mature 5.8S rRNA and may play a role in recruiting the RNA exosome complex to pre-rRNA; this function
may include C1D

Gene Wiki entry for MPHOSPH6


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000016.9  NC_018927.1  NT_010498.15  
Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for MPHOSPH6
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMPHOSPH6 promoter sequence
   Search SABiosciences Chromatin IP Primers for MPHOSPH6

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MPHOSPH6


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16q23.3   Ensembl cytogenetic band:  16q23.3   HGNC cytogenetic band: 16q23.3

MPHOSPH6 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MPHOSPH6 gene location

GeneLoc information about chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16M082181:  view genomic region     (about GC identifiers)

Start:
82,181,403 bp from pter      End:
82,203,831 bp from pter
Size:
22,429 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: MPH6_HUMAN, Q99547 (See protein sequence)
Recommended Name: M-phase phosphoprotein 6  
Size: 160 amino acids; 19024 Da
Subunit: Associates with the RNA exosome complex, probably mediated by EXOSC10. Interacts with ARHGAP18, EXOSC10 and
SKIV2L2
Subcellular location: Nucleus, nucleolus. Cytoplasm. Note=Cytoplasmic in M phase
Secondary accessions: B2RAF0

Explore the universe of human proteins at neXtProt for MPHOSPH6: NX_Q99547

Post-translational modifications:

  • Phosphorylated in M (mitotic) phase1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q99547

  • MPHOSPH6 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_005783.2  
    ENSEMBL proteins: 
     ENSP00000454996   ENSP00000258169   ENSP00000456626   ENSP00000457537   ENSP00000454900  

    Human Recombinant Protein Products: 
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    Novus Biologicals MPHOSPH6 Lysates
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    Browse ProSpec Recombinant Proteins
    Uscn Proteins for MPHOSPH6

    Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000176colocalizes with nuclear exosome (RNase complex) TAS11719186
    GO:0000178colocalizes with exosome (RNase complex) IDA11719186
    GO:0005634nucleus IDA--
    GO:0005730nucleolus IDA--
    GO:0005737cytoplasm IDA--


    MPHOSPH6 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    MPHOSPH6 for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR019324 MPP6

    Graphical View of Domain Structure for InterPro Entry Q99547

    ProtoNet protein and cluster: Q99547

    UniProtKB/Swiss-Prot: MPH6_HUMAN, Q99547
    Similarity: Belongs to the MPP6 family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: MPH6_HUMAN, Q99547
    Function: RNA-binding protein that associates with the RNA exosome complex. Involved in the 3'processing of the 7S
    pre-RNA to the mature 5.8S rRNA and may play a role in recruiting the RNA exosome complex to pre-rRNA; this function
    may include C1D

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    hsa-miR-1265
    SwitchGear 3'UTR luciferase reporter plasmidMPHOSPH6 3' UTR sequence
    Inhib. RNA
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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003723RNA binding IEA--
    GO:0005515protein binding IPI17412707


    MPHOSPH6 for ontologies           About GeneDecksing


    2 GenomeRNAi human phenotypes for MPHOSPH6:
     Decreased Tat-dependent transc  Synthetic lethal with Ras 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Deadenylation-dependent mRNA decay
    RNA degradation0.50

    Pathway sources
    See GeneCards unified pathways
    Show all pathways



    1         Kegg Pathway  (Kegg details for MPHOSPH6):
        RNA degradation


    MPHOSPH6 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for MPHOSPH6

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/49 Interacting proteins for MPHOSPH6 (Q995471, 2, 3 ENSP000002581694) via UniProtKB, MINT, STRING, and/or I2D (see all 49)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SKIV2L2P422851, 3, ENSP000002306404EBI-373187,EBI-347612 I2D: score=3 STRING: ENSP00000230640
    APLP1P516932, 3MINT-65273 I2D: score=5 
    C14orf1Q9UKR52, 3MINT-65278 I2D: score=5 
    GDF9O603832, 3MINT-65271 I2D: score=5 
    TP53P046372, 3MINT-65272 I2D: score=5 
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000087M phase of mitotic cell cycle TAS8885239
    GO:0000460maturation of 5.8S rRNA IMP17412707


    MPHOSPH6 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for MPHOSPH6
    Search CenterWatch for drugs/clinical trials and news about MPHOSPH6 / MPH6 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for MPHOSPH6 gene: 
    NM_005792.2  

    Unigene Cluster for MPHOSPH6:

    M-phase phosphoprotein 6
    Hs.344400  [show with all ESTs]
    Unigene Representative Sequence: BX537773
    8 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000563100 ENST00000258169(uc002fgw.3) ENST00000563504 ENST00000568016
    ENST00000569599 ENST00000566748 ENST00000569021 ENST00000567729

    miRNA
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    1 QIAGEN miScript miRNA Assays for microRNA that regulate MPHOSPH6:
    hsa-miR-1265
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    Inhib. RNA
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    Additional cDNA sequence: 

    AK222667.1 AK314163.1 BC005242.1 BC011020.1 BC029395.1 BC031017.1 BX537773.1 X98263.1 

    13 DOTS entries:

    DT.115950  DT.95091903  DT.100774055  DT.91748625  DT.120690833  DT.40123368  DT.426581  DT.100774042 
    DT.100774054  DT.101968302  DT.91746759  DT.99985608  DT.91727098 

    24/175 AceView cDNA sequences (see all 175):

    BQ011148 AA626333 BU629926 AI379304 AA476760 CK429207 AI248862 AW244131 
    AI473364 BE047811 AW149091 BM970535 CF528512 CA441038 BU622913 CD677695 
    AI831131 AA703558 CR611170 W86550 AA694019 AI032537 BM666297 BU595100 

    GeneLoc Exon Structure

    5/7 Alternative Splicing Database (ASD) splice patterns (SP) for MPHOSPH6 (see all 7)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6a · 6b · 6c ^ 7 ^ 8a · 8b · 8c · 8d
    SP1:                    -     -     -                 -     -           -     -                                 
    SP2:                    -           -                 -                                                         
    SP3:                                -                 -     -           -     -                                 
    SP4:                                                  -     -           -     -                                 
    SP5:                                            -     -     -           -     -                                 


    ECgene alternative splicing isoforms for MPHOSPH6

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    MPHOSPH6 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: CTCCCAGGTC

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See MPHOSPH6 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for MPHOSPH6

    SOURCE GeneReport for Unigene cluster: Hs.344400
        SABiosciences Custom PCR Arrays for MPHOSPH6
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MPHOSPH6

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for MPHOSPH6 gene from 6/19 species (see all 19)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Mphosph61 , 5 M phase phosphoprotein 61, 5 82.71(n)1
    87.5(a)1
      8 (65.09 cM)5
    685331  NM_026758.31  NP_081034.11 
     1177916455 
    chicken
    (Gallus gallus)
    Aves MPHOSPH61 M-phase phosphoprotein 6 76.87(n)
    76.87(a)
      415811  XM_414172.3  XP_414172.3 
    lizard
    (Anolis carolinensis)
    Reptilia MPHOSPH66
    --
    66(a)
    1 ↔ 1
    GL343663.1(16846-24313)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.73222 Xenopus laevis transcribed sequence with moderate similarity more 77.31(n)    AW636952.1 
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.157572 Transcribed sequence with weak similarity to protein more 76.27(n)    AL719826.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Mpp66
    M-phase phosphoprotein 6
    31(a)
    1 ↔ 1
    2L(21156874-21157685)


    ENSEMBL Gene Tree for MPHOSPH6 (if available)
    TreeFam Gene Tree for MPHOSPH6 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for MPHOSPH6 gene

    MPHOSPH6 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for MPHOSPH6
    PGOHUM00000262414


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/763 NCBI SNPs in MPHOSPH6 are shown (see all 763    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 16 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs99297021,2
    C,F,--82181316(+) gtcctA/Gtcttt 1 -- ds50014Minor allele frequency- G:0.34WA CSA 123
    rs124487851,2
    C,F,H,--82181402(+) ATGTAC/GCTCAT 1 -- ds50017Minor allele frequency- G:0.14NS NA WA EA 480
    rs99396651,2
    C,F,--82181403(+) TGTACC/TTCATT 1 -- ds50011Minor allele frequency- T:0.10WA 118
    rs1390669481,2
    --82181423(+) GGAGCA/GAAATT 1 -- ds50010--------
    rs124460301,2
    C,F,H,--82181505(+) ATTCTA/GTGTGC 1 -- ds50017Minor allele frequency- G:0.21NA EA 386
    rs788571141,2
    F,--82181563(+) TACCAT/CTGGTC 1 -- ds50011Minor allele frequency- C:0.02WA 118
    rs1863298931,2
    --82181573(+) CAGTGA/GTAAAT 1 -- ds50010--------
    rs124486721,2
    C,F,A,H,--82181582(+) ATTCTG/ATTGGG 1 -- ds500123Minor allele frequency- A:0.18NS EA NA WA 2768
    rs1896089991,2
    --82181595(+) ATACCA/GTAAGA 1 -- ds50010--------
    rs99392181,2
    C,F,H,--82181608(+) CTAAAG/AGTTAT 1 -- ds50019Minor allele frequency- A:0.07NS EA NA WA 1118

    HapMap Linkage Disequilibrium report for MPHOSPH6 (82181403 - 82203831 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for MPHOSPH6
         1 Indel: 60221

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing MPHOSPH6
    DNA2.0 Custom Variant and Variant Library Synthesis for MPHOSPH6

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    MPHOSPH6 for disorders           About MalaCards

    MPHOSPH6 for disorders           About GeneDecksing

    OMIM gene information: 605500    OMIM disorders: --

    Human Genome Epidemiology (HuGE) Navigator: MPHOSPH6 (2 documents)

    Export disorders for MPHOSPH6 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for MPHOSPH6 gene, integrated from 9 sources (see all 26):
    (articles sorted by number of sources associating them with MPHOSPH6)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification of novel M phase phosphoproteins by expression cloning. (PubMed id 8885239)1, 2, 3 Matsumoto-Taniura N....Westendorf J.M. (1996)
    2. MPP6 is an exosome-associated RNA-binding protein involved in 5.8S rRNA maturation. (PubMed id 16396833)1, 2, 9 Schilders G....Pruijn G.J. (2005)
    3. Dis3-like 1: a novel exoribonuclease associated with the human exosome. (PubMed id 20531389)1, 2 Staals R.H....Pruijn G.J. (2010)
    4. C1D and hMtr4p associate with the human exosome subunit PM/Scl-100 and are involved in pre-rRNA processing. (PubMed id 17412707)1, 2 Schilders G.... Pruijn G.J.M. (2007)
    5. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)1, 2 Olsen J.V....Mann M. (2006)
    6. A protein interaction framework for human mRNA degradation. (PubMed id 15231747)1, 2 Lehner B. and Sanderson C.M. (2004)
    7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    8. AU binding proteins recruit the exosome to degrade ARE-containing mRNAs. (PubMed id 11719186)1, 2 Chen C.-Y.... Karin M. (2001)
    9. A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
    10. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 10200 HGNC: 7214 AceView: MPHOSPH6 Ensembl:ENSG00000135698 euGenes: HUgn10200
    ECgene: MPHOSPH6 Kegg: 10200 H-InvDB: MPHOSPH6

    (According to HUGE)
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
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    NameDescription
    PharmGKB entry for MPHOSPH6 Pharmacogenomics, SNPs, Pathways

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