Home
GeneCards Guide
Suite
- MalaCards
- GeneALaCart
- GeneDecks
- GeneIP
- GeneLoc
Terms and Conditions
- Terms of Use
- 3rd Party Notice
About Us
User Feedback
Mirror sites

Set Analyses:

Generates a file of GeneCards annotations for your list of genes

Advanced Search

Search By

Section (entire)

for

All GeneCards Within gene subset (to enable gene prioritization):
or upload a file of gene symbols

Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

MMP12 Gene

protein-coding GIFtS: 70
GCID: GC11M102767

matrix metallopeptidase 12 (macrophage elastase)

(Previous name: matrix metalloproteinase 12 (macrophage elastase) )

Explore 82 diseases affiliated with
MMP12 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Matrix Metallopeptidase 12 (Macrophage Elastase)¹ ²		EC 3.4.24.65³ ⁸
HME¹ ² ³		Macrophage Metalloelastase²
Matrix Metalloproteinase 12 (Macrophage Elastase)¹ ²		HME¹ ² ³
ME² ³		Macrophage Elastase³
MME² ³		Matrix Metalloproteinase-12³
MMP-12² ³		EC 3.4.24⁸

External Ids:	HGNC: 7158¹	Entrez Gene: 4321²	Ensembl: ENSG00000110347⁷	OMIM: 601046⁵	UniProtKB: P39900³

Export aliases for MMP12 gene to outside databases

Previous GC identifers: GC11M104927 GC11M104090 GC11M102271 GC11M102238 GC11M098661

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for MMP12:

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal

physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease

processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when

cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to

yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble

elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema.

The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: MMP12_HUMAN, P39900

Function: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and

small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at

the P1 site, with small hydrophobic residues (preferably alanine) occupying P3

summary for MMP12:

Matrix metalloproteases (matrix metalloproteinase, MMPs), also called matrixins, are zinc-dependent

endopeptidases that are the major proteases involved in ECM degradation. MMPs are capable of degrading a

wide range of extracellular molecules and a number of bioactive molecules. 24 matrixin genes have been

identified in humans, which can be organized into six groups based on domain organization and substrate

preference: Collagenases (MMP-1, -8 and -13), Gelatinases (MMP-2 and MMP-9), Stromelysins (MMP-3, -10 and

-11), Matrilysin (MMP-7 and MMP-26), Membrane-type (MT)-MMPs (MMP-14, -15, -16, -17, -24 and -25) and others

(MMP-12, -19, -20, -21, -23, -27 and -28). MMP activity is regulated by two major endogenous inhibitors:

alpha2-macroglobulin and tissue inhibitors of metalloproteases (TIMPs). MMPs play a central role in cell

proliferation, migration, differentiation, angiogenesis, apoptosis and host defences. Dysregulatoin of MMPs

has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis and cancer.

Tumour metastasis is a multistep process involving the dessemination of tumor cells from the primary tumor

to secondarys at a distant organ or tissue. One of the first steps in metastasis is the degradation of the

basement membrane, a process in which MMPs have been implicated. MMPs are secreted by tumor cells themselves

or by surrounding stromal cells stimulated by the nearby tumor. Numerous studies have linked altered MMP

expression in different human cancers with poor disease prognosis. MMP-1, -2, -3, -7, -9, -13 and -14 all

have elevated expression in primary tumors and/or metastases. Synthetic or natural inhibitors of MMPs result

in inhibition of metastasis, while up-regulation of MMPs led to enhanced cancer cell invasion.

Gene Wiki entry for MMP12 (Matrix metallopeptidase 12)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000011.9 NC_018922.1 NT_033899.8

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the MMP12 gene promoter:
RFX1 Sox5 GATA-3 STAT5A HNF-3beta GATA-2 GATA-1 RORalpha1 Meis-1a Meis-1
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: MMP12 promoter sequence

Search SABiosciences Chromatin IP Primers for MMP12

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MMP12

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11q22.3 Ensembl cytogenetic band: 11q22.2 HGNC cytogenetic band: 11q22.3

MMP12 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MMP12 gene location

GeneLoc information about chromosome 11 GeneLoc Exon Structure

GeneLoc location for GC11M102767: view genomic region (about GC identifiers)

Start:	102,733,464 bp from pter	End:	102,745,764 bp from pter
Size:	12,301 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: MMP12_HUMAN, P39900 (See protein sequence)

Recommended Name: Macrophage metalloelastase precursor

Size: 470 amino acids; 54002 Da

Cofactor: Binds 4 calcium ions per subunit

Cofactor: Binds 2 zinc ions per subunit

Subcellular location: Secreted, extracellular space, extracellular matrix (Probable)

6/54 PDB 3D structures from and Proteopedia for MMP12 (see all 54):

1JIZ (3D)

1JK3 (3D)

1OS2 (3D)

1OS9 (3D)

1RMZ (3D)

1ROS (3D)

Secondary accessions: B2R9X8 B7ZLF6 Q2M1L9

Explore the universe of human proteins at neXtProt for MMP12: NX_P39900

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_P39900

4/5 DME Specific Peptides for MMP12 (P39900) (see all 5)

FKDRFFW

GIQSLYG

VKKIDAA

WSNVTPL

MMP12 Protein expression data from MOPED and PaxDb: --

REFSEQ proteins: NP_002417.2 Reactome Protein details: P39900
Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
	R&D Systems Recombinant & Natural Proteins for MMP12 (MMP-12)
	Enzo Life Sciences proteins for MMP12
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells OriGene Protein Over-expression Lysate: MMP12 OriGene Custom Protein Services for MMP12
	GenScript Custom Purified and Recombinant Proteins Services for MMP12
	Novus Biologicals MMP12 Proteins Novus Biologicals MMP12 Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for MMP12

Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005578	proteinaceous extracellular matrix	IEA	--

MMP12 for ontologies About GeneDecksing

MMP12 Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of MMP12
	R&D Systems Antibodies for MMP12 (MMP-12)
	OriGene Antibodies: MMP12 OriGene Custom Antibody Services for MMP12
	GenScript Custom Superior Antibodies Services for MMP12
	Novus Biologicals MMP12 Antibodies
	Abcam antibodies for MMP12
	Uscn Antibodies for MMP12
	ThermoFisher Antibodies for MMP12

Assay Products for MMP12:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	R&D Systems Multiplex/Array Assay Kits & Reagents for MMP12 (MMP-12)
	GenScript MMP12-Activity-based Protease Assay for Compound Screening
	Enzo Life Sciences assays for MMP12
	Uscn ELISAs and CLIAs for MMP12

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

MMP12 for domains About GeneDecksing

5/10 InterPro domains/families (see all 10):

IPR024079 MetalloPept_cat_dom

IPR021158 Pept_M10A_Zn_BS

IPR021190 Pept_M10A_matrixin

IPR000585 Hemopexin/matrixin

IPR018486 Hemopexin/matrixin_CS

Graphical View of Domain Structure for InterPro Entry P39900

ProtoNet protein and cluster: P39900

2 Blocks protein families:

IPB000585 Hemopexin repeat

IPB001818 Matrixin

UniProtKB/Swiss-Prot: MMP12_HUMAN, P39900

Domain: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the

enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme

Similarity: Belongs to the peptidase M10A family

Similarity: Contains 4 hemopexin-like domains

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

UniProtKB/Swiss-Prot: MMP12_HUMAN, P39900

Function: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and

small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at

the P1 site, with small hydrophobic residues (preferably alanine) occupying P3

Catalytic activity: Hydrolysis of soluble and insoluble elastin. Specific cleavages are also produced at 14-Ala-

-Leu-15 and 16-Tyr- -Leu-17 in the B chain of insulin

Induction: By exposure to bacterial lipopolysaccharides (LPS). Inhibited by dexamethasone

Genatlas biochemistry entry for MMP12:

matrix metalloproteinase 12,regulator in matrix remodeling in the MMP cluster,expressed in alveolar macrophage and

placental cells,mRNA upregulated in rheumatoid arthritis

Enzyme Numbers (IUBMB): EC 3.4.24.65¹ ² EC 3.4.24²

miRNA Products:		OriGene 3'-UTR Clone: MMP12 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat MMP12
		Search QIAGEN for miScript miRNA Assays for microRNAs that regulate MMP12
		SwitchGear 3'UTR luciferase reporter plasmid: MMP12 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for MMP12 (see all 7) OriGene shRNA RFP: MMP12 OriGene siRNA: MMP12
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat MMP12

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for MMP12

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MMP12 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MMP12 OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: MMP12 (NM_002426)
		Sino Biological Human cDNA Clone for MMP12
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for MMP12
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MMP12

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for MMP12
		Search LifeMap BioReagents cell lines for MMP12

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MMP12

Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0004175	endopeptidase activity	TAS	8226919
GO:0004222	metalloendopeptidase activity	TAS	8226919
GO:0005509	calcium ion binding	IEA	--
GO:0008270	zinc ion binding	IEA	--

MMP12 for ontologies About GeneDecksing

2 GenomeRNAi human phenotypes for MMP12:

Cell division defect

Increased 8N DNA content

Animal Models:
Mouse knock-out Mmp12^tm1Sds for MMP12
8 MGI mutant phenotypes (inferred from 2 alleles) (MGI details for Mmp12):

cardiovascular system	hematopoietic system	homeostasis/metabolism	immune system	integument
nervous system	reproductive system	respiratory system

MMP12 for phenotypes About GeneDecksing

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways - 5/8 super-pathways (see all 8) About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Cell adhesion_ECM remodeling

	Cell adhesion_ECM remodeling	1.00			Metalloproteases in connective tissue degradation	0.31
	Cell adhesion ECM remodeling	1.00

Degradation of the extracellular matrix

Degradation of the extracellular matrix

1.00

Degradation of collagen

0.79

Matrix Metalloproteinases

Matrix Metalloproteinases

1.00

Inhibition of Matrix Metalloproteinases

0.68

Integrin Pathway

UPA-UPAR Pathway

0.51

Transendothelial Migration of Leukocytes

0.38

Colorectal Cancer Metastasis

Colorectal Cancer Metastasis

1.00

Pathway sources
See GeneCards unified pathways
Show all pathways

2 EMD Millipore Pathways for MMP12

	Cell adhesion ECM remodeling
	Metalloproteases in connective tissue degradation

5 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for MMP12

	Inhibition of Matrix Metalloproteinases
	Colorectal Cancer Metastasis
	Transendothelial Migration of Leukocytes
	UPA-UPAR Pathway
	Pancreatic Adenocarcinoma

1 GeneGo (Thomson Reuters) Pathway for MMP12

Cell adhesion ECM remodeling

1 BioSystems Pathway for MMP12

Matrix Metalloproteinases

4 Reactome Pathways for MMP12

	Extracellular matrix organization
	Gelatin degradation by MMP1, 2, 3, 7, 8, 9, 12, 13
	Degradation of the extracellular matrix
	Degradation of collagen

MMP12 for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for MMP12

5/16 Interacting proteins for MMP12 (P39900^{2, 3}) via UniProtKB, MINT, STRING, and/or I2D (see all 16)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
CXCL2	P19875³	I2D: score=2
F12	P00748³	I2D: score=2
PLAUR	Q03405³	I2D: score=2
TFPI	P10646³	I2D: score=2
CCL13	Q99616³	I2D: score=1

About this table

Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006508	proteolysis	IEA	--
GO:0035313	wound healing, spreading of epidermal cells	IDA	15802269
GO:0060054	positive regulation of epithelial cell proliferation involved in wound healing	IDA	15802269

MMP12 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

MMP12 for compounds About GeneDecksing

EMD Millipore small molecules for MMP12:
Small Molecule - inhibitor

Enzo Life Sciences drugs & compounds for MMP12

Compounds for MMP12 available from Tocris Bioscience About this table

Compound	Action	CAS #
Marimastat	Broad spectrum MMP inhibitor	[154039-60-8]
ONO 4817	Broad spectrum MMP inhibitor	[223472-31-9]
Batimastat	Potent, broad spectrum MMP inhibitor	[130370-60-4]
PD 166793	Broad spectrum MMP inhibitor	[199850-67-4]
UK 370106	Highly selective MMP-3 and MMP-12 inhibitor	[230961-21-4]

1 HMDB Compound for MMP12 About this table

Compound	Synonyms	CAS #	PubMed Ids
Calcium	Ca (see all 2)	7440-70-2	--

10/15 DrugBank Compounds for MMP12 (see all 15) About this table

Compound	Synonyms	CAS #	Type	Actions	PubMed Ids
Acetohydroxamic Acid	Acethydroxamsaure (see all 10)	546-88-3	target	inhibitor	16959356 16899369 15809432 17269766
Batimastat	--	130370-60-4	target	--	17139284 17016423 10592235
CP-271485	(6R)-4-BENZYL-6-(1-METHYL-2,2-DIOXIDO-1,3-DIHYDRO-2,1-BENZISOTHIAZOL-5-YL)MORPHOLIN-3-ONE (see all 2)	--	target	--	17139284 17016423 10592235
PF-00356231	--	--	target	--	17139284 17016423 10592235
2-[2-(1,3-Dioxo-1,3-Dihydro-2h-Isoindol-2-Yl)Ethyl]-4-(4'-Ethoxy-1,1'-Biphenyl-4-Yl)-4-Oxobutanoic Acid	--	--	target	--	17139284 17016423
Marimastat	--	154039-60-8	target	inhibitor	10852638 10669951
(1S,5S,7R)-N~7~-(BIPHENYL-4-YLMETHYL)-N~3~-HYDROXY-6,8-DIOXA-3-AZABICYCLO[3.2.1]OCTANE-3,7-DICARBOXAMIDE	--	--	target	--	10592235
2-[(4-fluorophenyl)sulfonylamino]-N-oxo-ethanamide	--	--	target	--	10592235
N-(biphenyl-4-ylsulfonyl)-D-leucine	--	--	target	--	10592235
N-(dibenzo[b,d]thiophen-3-ylsulfonyl)-L-valine	--	--	target	--	10592235

10/21 Novoseek chemical compound relationships for MMP12 gene (see all 21) About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
marimastat	66	1	17234180 (1)
acetohydroxamic acid	63	3	15809432 (1), 16899369 (1)
batimastat	52.7	2	15809432 (1), 11575928 (1)
hydroxamate	51.2	5	11575929 (2), 12504900 (1), 16674108 (1)
zinc	26.9	9	15289103 (2), 12504900 (1), 11575929 (1), 16737445 (1)
matrigel	20.5	1	16917801 (1)
vegf	19	14	10636104 (4), 19251952 (1), 17981559 (1), 11740820 (1) (see all 7)
etanercept	8.96	1	15781250 (1)
serine	4.41	2	9218437 (1)
lipid	2.88	1	18823978 (1)

Search CenterWatch for drugs/clinical trials and news about MMP12

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for MMP12 gene:

NM_002426.4

Unigene Cluster for MMP12:

Matrix metallopeptidase 12 (macrophage elastase)

Hs.1695 [show with all ESTs]

Unigene Representative Sequence: NM_002426

2 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000532855(lincRNA)(uc001phk.3) ENST00000326227(lincRNA)

miRNA Products:		OriGene 3'-UTR Clone: MMP12 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat MMP12
		Search QIAGEN for miScript miRNA Assays for microRNAs that regulate MMP12
		SwitchGear 3'UTR luciferase reporter plasmid: MMP12 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for MMP12 (see all 7) OriGene shRNA RFP: MMP12 OriGene siRNA: MMP12
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat MMP12

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MMP12 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MMP12 OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: MMP12 (NM_002426)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for MMP12
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MMP12

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for MMP12 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat MMP12
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat MMP12
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MMP12

Additional cDNA sequence:

AK313959.1 BC112301.1 BC143773.1 L23808.1

3 DOTS entries:

DT.310810 DT.91727117 DT.95263647

24/67 AceView cDNA sequences (see all 67):

BQ011671 AL552270 AV716402 AL552239 AW170498 CR603756 CR594246 BE466604

AI368844 BQ011440 BQ012914 CB241588 BX377010 N30838 N41366 L23808

BM831899 AL569775 N41372 AA639771 AI203356 AW197615 BG215020 BE440047

GeneLoc Exon Structure

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

MMP12 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: CTCTGTAAGT

About this image

MMP12 expression in embryonic tissues and stem cells

Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

3 LifeMap In Vivo Development Anatomical Compartments/Cells

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Placenta

Extravillous Cytotrophoblast Layer

Endovascular Cytotrophoblast Cells

Extraembryonic Tissues, Placenta

Placenta

Extravillous Cytotrophoblast Layer

Interstitial Cytotrophoblast Cells

Extraembryonic Tissues, Placenta

Adipose

Visceral White Adipose

Adipose

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

Genevestigator expression for MMP12

SOURCE GeneReport for Unigene cluster: Hs.1695

UniProtKB/Swiss-Prot: MMP12_HUMAN, P39900

Tissue specificity: Found in alveolar macrophages but not in peripheral blood monocytes

SABiosciences Expression via Pathway-Focused PCR Arrays including MMP12:

Lung Cancer in human mouse rat

Extracellular Matrix & Adhesion Molecules in human mouse rat

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for MMP12 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat MMP12
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat MMP12
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MMP12

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MMP12

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of human and mouse.

Orthologs for MMP12 gene from 1/6 species (see all 6) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Mmp12^{1 , 5}	matrix metallopeptidase 12^{1, 5}	75.7(n)¹ 63.34(a)¹		9 (2.46 cM)⁵ 17381¹ NM_008605.3¹ NP_032631.3¹ 7344381⁵

ENSEMBL Gene Tree for MMP12 (if available)
TreeFam Gene Tree for MMP12 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 11 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs28381704^1,2	C,	--	98660993(-)	`CCCTGA/TCACAT`	1	--	ds500¹		5		NS	190
rs28381703^1,2	C,F,	--	98661253(-)	`AGTGCT/CTACAG`	1	--	ds500¹		6		NS WA	308
rs28381702^1,2	C,F,	--	98661488(-)	`AAAAA-/ATCATT`	1	--	ut3¹		5		NS	188
rs56184183^1,2	C	--	98661833(+)	`CAAAAA/CCCATT`	1	--	ut3¹		0	--	--	--	--
rs28381701^1,2	C	--	98661860(-)	`GGTTTC/G/TGTTGT`	3	R G C	mis¹ ese³		7		NS NA	190
rs143740509^1,2	C,	--	98661966(+)	`GAAAAC/GAAATT`	1	--	int¹		0	--	--	--	--
rs28381700^1,2	C,	--	98662076(-)	`TAAGGG/ACATGA`	1	--	int¹		5		NS	184
rs28381699^1,2	C,	--	98662161(-)	`ATGAGG/ATCTTG`	1	--	int¹		5		NS	178
rs28381698^1,2	C,F,	--	98662225(-)	`TTTATG/ATCCTC`	1	--	int¹		6		NS WA	304
rs28381696^1,2	C,	--	98662287(-)	`ACTTGG/AAGTAT`	1	--	int¹		5		NS	188

HapMap Linkage Disequilibrium report for MMP12 (102733464 - 102745764 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for MMP12: --
Human Gene Mutation Database (HGMD): MMP12

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing MMP12

DNA2.0 Custom Variant and Variant Library Synthesis for MMP12

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

MMP12 for disorders           About GeneDecksing

OMIM gene information: 601046    OMIM disorders: --

20/82 diseases for MMP12 (see all 82):    About MalaCards

emphysema alpha 1-antitrypsin deficiency encephalomyelitis arthritis

refractive error dermatitis herpetiformis aortic aneurysm abdominal aortic aneurysm

gastric cardia adenocarcinoma arteriovenous malformation pulmonary emphysema anaplastic astrocytoma

chronic obstructive pulmonary disease esophageal squamous cell carcinoma ollier disease squamous cell carcinoma

aggressive periodontitis celiac disease exostosis moyamoya disease

3 diseases from the University of Copenhagen DISEASES database for MMP12:

Chronic obstructive pulmonary disease Ehrlichiosis Exostosis

10/48 Novoseek disease relationships for MMP12 gene (see all 48) About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
pulmonary disease chronic obstructive	63.9	17	18619044 (2), 15355665 (1), 19775099 (1), 15723202 (1) (see all 8)
pulmonary emphysema	63.7	2	19456279 (1), 9082500 (1)
solar elastosis	59.4	1	12190877 (1)
inflammation	44.3	24	16510458 (3), 19725580 (2), 18001475 (2), 19706765 (1) (see all 14)
aortic aneurysm abdominal	43.8	6	9835614 (1), 16122719 (1), 16082623 (1), 17182940 (1) (see all 5)
atherosclerotic plaque	40.6	4	10825169 (1), 15073384 (1), 17194896 (1), 18823978 (1)
bronchoalveolar lavage fluid	36.5	2	9230755 (1)
atherosclerosis	35.1	28	18403602 (5), 10825169 (2), 15359603 (1), 19456279 (1) (see all 10)
dermatitis herpetiformis	32.3	3	10201535 (1), 11247893 (1)
metastasis	26.9	16	15709175 (2), 18396640 (2), 17357518 (2), 11923608 (2) (see all 9)

Genetic Association Database (GAD): MMP12
Human Genome Epidemiology (HuGE) Navigator: MMP12 (59 documents)

Export disorders for MMP12 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for MMP12 gene, integrated from 9 sources (see all 358):
(articles sorted by number of sources associating them with MMP12)

Utopia: connect your pdf to the dynamic
world of online information

Crystal structure of human macrophage elastase (MMP-12) in complex with a hydroxamic acid inhibitor. (PubMed id 11575929)^{1, 2, 9} Nar H.... Jung B. (2001)
Substrate specificity determinants of human macrophage elastase (MMP- 12) based on the 1.1 A crystal structure. (PubMed id 11575928)^{1, 2, 9} Lang R....Maskos K. (2001)
Hydrolysis of a broad spectrum of extracellular matrix proteins by human macrophage elastase. (PubMed id 9115292)^{1, 2, 9} Gronski T.J. Jr.... Shapiro S.D. (1997)
Cloning and characterization of a unique elastolytic metalloproteinase produced by human alveolar macrophages. (PubMed id 8226919)^{1, 2, 9} Shapiro S.D.... Ley T.J. (1993)
Genotype-phenotype relationships in an investigation of the role of proteases in abdominal aortic aneurysm expansion. (PubMed id 16082623)^{1, 4, 9} Eriksson P....Powell J.T. (2005)
The role of matrix metalloproteinase polymorphisms in the rate of decline in lung function. (PubMed id 11875051)^{1, 4, 9} Joos L....Sandford A.J. (2002)
Genotypes and haplotypes of matrix metalloproteinase 1, 3 and 12 genes and the risk of lung cancer. (PubMed id 16311244)^{1, 4, 9} Su L....Christiani D.C. (2006)
Polymorphisms in matrix metalloproteinase-1, -3, -9, and -12 genes in relation to subarachnoid hemorrhage. (PubMed id 11546917)^{1, 4, 9} Zhang B....Ye S. (2001)
Genetic polymorphisms in the matrix metalloproteinase 12 gene (MMP12) and breast cancer risk and survival: the Shanghai Breast Cancer Study. (PubMed id 15987457)^{1, 4} Shin A....Zheng W. (2005)
Genetic analysis of polymorphisms in biologically relevant candidate genes in patients with abdominal aortic aneurysms. (PubMed id 15944607)^{1, 4} Ogata T....Kuivaniemi H. (2005)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 4321	HGNC: 7158	AceView: MMP12	Ensembl:ENSG00000110347	euGenes: HUgn4321
ECgene: MMP12	H-InvDB: MMP12

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for MMP12	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for MMP12	Genetics and Cytogenetics in Oncology and Haematology
NIEHS-SNPs	http://egp.gs.washington.edu/data/mmp12/

Intellectual Property for MMP12 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

Patent Information for MMP12 gene:
Search GeneIP for patents involving MMP12

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section

	Browse Kits and Assays available from EMD Millipore
	EMD Millipore Mono- and Polyclonal Antibodies for the study of MMP12
	EMD Millipore small molecules (inhibitor) for MMP12
	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
	Browse for Gene Knock-down Tools from EMD Millipore

	EMD Millipore Custom Antibody & Bulk Services
	EMD Millipore Preclinical / Clinical Development Services
	EMD Millipore Immunoassay Services
	EMD Millipore Target Screening & Profiling Services

	Antibodies for MMP12 (MMP-12)		Browse Cell Culture Products
	Browse ELISAs		Browse Flow Cytometry Kits
	Browse Primer Pairs		Browse Kinase Activity Assays/Reagents
	Browse ELISpot Kits/Development Modules		Browse TFB/Immunoprecipitation Assays
	Browse Apoptosis Detection Kits/Reagents		Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents
	Browse DNA Damage/Repair Kits/Reagents		Multiplex/Array Assay Kits/Reagents for MMP12 (MMP-12)
	Browse Cell Selection/Detection Kits/Reagents		Browse Secondary Antibodies/Controls/Staining Reagents
	Browse Phosphatase Activity Assays/Reagents		Recombinant/Natural Proteins for MMP12 (MMP-12)

	OriGene Antibodies for MMP12		OriGene shRNA RFP for MMP12
	OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for MMP12		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for MMP12
	OriGene Protein Over-expression Lysate for MMP12		Browse OriGene Fluorogenic Cell Assay Kits
	OriGene siRNA for MMP12		OriGene 3'-UTR Clone for MMP12
	OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MMP12		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MMP12
	Browse OriGene GFP tagged cDNA clones in CMV expression vector		Browse OriGene MicroRNA Expression Plasmids
	Browse OriGene basic RS shRNAs		Browse OriGene validated miRNA SYBR primer pairs
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells		OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
	OriGene Custom Antibody Services for MMP12		OriGene Custom Protein Services for MMP12
	OriGene Custom Immunoassay Development

	QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat MMP12
	QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing MMP12
	QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MMP12
	QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat MMP12
	QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MMP12
	QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat MMP12

	GenScript Custom Purified and Recombinant Proteins Services for MMP12		GenScript cDNA clones with any tag delivered in your preferred vector for MMP12
	GenScript MMP12-Activity-based typehere Assay for Compound Screening		GenScript Custom Superior Antibodies Services for MMP12
	GenScript Custom overexpressing Cell Line Services for MMP12		CloneReady with Over 120,000 Genes
	Gene Synthesis: Any Gene in Any Vector		Vector-based siRNA and miRNA, Ready for Transfection
	Gene Mutant Library, Variants up to 10^11		Plasmid Preparation
	Custom Peptide Services

Search for Antibodies & Assays

	Regulatory tfbs in MMP12 promoter
	Search Chromatin IP Primers for MMP12
	RT² qPCR Primer Assay in human, mouse, rat MMP12
	GNC Network for MMP12
	SABiosciences PCR Arrays including human, mouse, rat MMP12

Tocris compounds for MMP12

	Browse Sino Biological Proteins and Antibodies
	cDNA Clones for MMP12
	Antibodies/Proteins Production Services
	Rabbit Monoclonal Antibody Platform
	Bulk Purchasing

	Assays/Drugs/Proteins for MMP12
	Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

	Novus Tissue Slides
	MMP12 antibodies
	MMP12 proteins
	MMP12 lysates

	Antibodies for MMP12
	See all of Abcam's Antibodies, Kits and Proteins for MMP12
	Custom Antibody / Protein Production Service
	Bulk Purchasing
	Advantages of Rabbit Monoclonal antibodies
	Abcam protocols and scientific support

Browse ProSpec Recombinant Proteins

MMP12 Proteins, Antibodies, CLIAs, and ELISAs

Search LifeMap BioReagents cell lines for MMP12

	Gene Synthesis
	Protein Engineering
	Variant Library Synthesis
	Codon Optimization
	Protein Production and Purification

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MMP12

	SwitchGear 3'UTR luciferase reporter plasmids for MMP12
	SwitchGear Promoter luciferase reporter plasmids for MMP12

ThermoFisher Antibodies for MMP12

Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MMP12

GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

View Random Gene

Category			VWF (GIFTS: 73) von Willebrand factor
GIFtS Group

The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

The GeneCards Human Gene Database

Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

Hot genes Disease genes MMP12 gene at Home site.

hostname: 356977-web1.xennexinc.com index build: 100 solr: 1.4

Advanced Search

Export list of displayed genes

MMP12 Gene

matrix metallopeptidase 12 (macrophage elastase)

Aliases
for MMP12 gene

Summaries
for MMP12 gene

Genomic Views
for MMP12 gene

Proteins
for MMP12 gene

Protein Domains /
Families
for MMP12 gene

Function
for MMP12 gene

Pathways & Interactions
for MMP12 gene

Drugs & Compounds
for MMP12 gene

Transcripts
for MMP12 gene

Expression
for MMP12 gene

Orthologs
for MMP12 gene

Paralogs
for MMP12 gene

Genomic Variants
for MMP12 gene

Disorders / Diseases
for MMP12 gene

Publications
for MMP12 gene

External Searches for MMP12 gene

Genome Databases showing MMP12 gene

Other Databases showing MMP12 gene

Specialized Databases showing MMP12 gene

Intellectual Property
for MMP12 gene

Products
for MMP12 gene

View Random Gene

Encephalomyelitis
Melanoma
bladder cancer
Pulmonary Emphysema
Glioma
Hyperplasia
Demyelination
atherosclerotic plaque
Carcinoma Basal Cell
Dermatitis Herpetiformis
Squamous Cell Cancer
Carcinoma
aggressiveness
Osteoarthritis
Solar elastosis
Anaplastic Astrocytoma
Atherosclerosis
Breast Cancer
NSCLC
Asthma
Adenocarcinoma
HEPATOCELLULAR CARCINOMA
Cancer
Aneurysm
Cancer Lung
Carcinoma Lewis Lung
Coronary Artery Disease
Meningioma
Aortic Aneurysm Abdominal
Bronchoalveolar Lavage Fluid
Skin Cancer
Colorectal Cancer
Multiple Sclerosis
Inflammation
Carcinoma Squamous Cell
Lung diseases
Pulmonary Disease Chronic Obstructive
STROKE
Rheumatoid Arthritis
Pneumonia
Tumors
Granuloma
Metastasis
Necrosis
Celiac Disease
Sclerosis Systemic
Glioblastoma
Arthritis

Advanced Search

Export list of displayed genes

MMP12 Gene

matrix metallopeptidase 12 (macrophage elastase)

Aliasesfor MMP12 gene

Summariesfor MMP12 gene

Genomic Viewsfor MMP12 gene

Proteinsfor MMP12 gene

Protein Domains /Familiesfor MMP12 gene

Functionfor MMP12 gene

Pathways & Interactionsfor MMP12 gene

Drugs & Compoundsfor MMP12 gene

Transcriptsfor MMP12 gene

Expression for MMP12 gene

Orthologsfor MMP12 gene

Paralogsfor MMP12 gene

Genomic Variantsfor MMP12 gene

Disorders / Diseasesfor MMP12 gene

Publicationsfor MMP12 gene

External Searches for MMP12 gene

Genome Databases showing MMP12 gene

Other Databases showing MMP12 gene

Specialized Databases showing MMP12 gene

Intellectual Propertyfor MMP12 gene

Productsfor MMP12 gene

View Random Gene

Aliases
for MMP12 gene

Summaries
for MMP12 gene

Genomic Views
for MMP12 gene

Proteins
for MMP12 gene

Protein Domains /
Families
for MMP12 gene

Function
for MMP12 gene

Pathways & Interactions
for MMP12 gene

Drugs & Compounds
for MMP12 gene

Transcripts
for MMP12 gene

Expression
for MMP12 gene

Orthologs
for MMP12 gene

Paralogs
for MMP12 gene

Genomic Variants
for MMP12 gene

Disorders / Diseases
for MMP12 gene

Publications
for MMP12 gene

Intellectual Property
for MMP12 gene

Products
for MMP12 gene