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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

MLST8 Gene

protein-coding   GIFtS: 56
GCID: GC16P002254

MTOR Associated Protein, LST8 Homolog (S. Cerevisiae)

Alzheimer's & Parkinson's Diseases Congress
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
MTOR Associated Protein, LST8 Homolog (S. Cerevisiae)1 2     G Protein Beta Subunit Like1
GBL2 3 5     GbetaL2
LST82 3 5     gable2
Mammalian Lethal With SEC13 Protein 82 3     Target Of Rapamycin Complex Subunit LST82
Protein GbetaL2 3     Gable3
TORC Subunit LST82 3     G Protein Beta Subunit-Like3
POP32 5     mLST83
WAT12 5     

External Ids:    HGNC: 248251   Entrez Gene: 642232   Ensembl: ENSG000001679657   OMIM: 6121905   UniProtKB: Q9BVC43   

Export aliases for MLST8 gene to outside databases

Previous GC identifers: GC16P002196 GC16P002255 GC16P002182


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

GeneCards Summary for MLST8 Gene: 
MLST8 (MTOR associated protein, LST8 homolog (S. cerevisiae)) is a protein-coding gene. Diseases associated with MLST8 include mental disorders, and human t-cell leukemia virus type 1, and among its related super-pathways are Energy dependent regulation of mTOR by LKB1-AMPK and Signaling by FGFR. GO annotations related to this gene include protein binding.

UniProtKB/Swiss-Prot: LST8_HUMAN, Q9BVC4
Function: Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and
hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated
mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the
RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1
requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated
mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome
synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E
(eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by
phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and
enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors
RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be
nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton,
probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the
serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473'
phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1
which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2
also modulates the phosphorylation of PRKCA on 'Ser-657'

Gene Wiki entry for MLST8 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000016.9  NT_010393.16  NC_018927.2  
Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for MLST8
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): MLST8 promoter sequence
   Search SABiosciences Chromatin IP Primers for MLST8

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MLST8


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16p13.3   Ensembl cytogenetic band:  16p13.3   HGNC cytogenetic band: 16p13.3

MLST8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MLST8 gene location

GeneLoc information about chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16P002254:  view genomic region     (about GC identifiers)

Start:
2,254,249 bp from pter      End:
2,259,418 bp from pter
Size:
5,170 bases      Orientation:
plus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: LST8_HUMAN, Q9BVC4 (See protein sequence)
Recommended Name: Target of rapamycin complex subunit LST8  
Size: 326 amino acids; 35876 Da
Subunit: Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR,
AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Part of the mammalian target of
rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1,
mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Interacts directly with MTOR and RPTOR.
Interacts with RHEB
Subcellular location: Cytoplasm (By similarity)
Sequence caution: Sequence=EAW85539.1; Type=Erroneous gene model prediction;
6 PDB 3D structures from and Proteopedia for MLST8:
4JSN (3D)        4JSP (3D)        4JSV (3D)        4JSX (3D)        4JT5 (3D)        4JT6 (3D)    
Secondary accessions: B3KMM4 B4DY00 D3DU88 Q5M800 Q86Y18 Q8WUI5 Q9HA66 Q9UJV6
Alternative splicing: 4 isoforms:  Q9BVC4-1   Q9BVC4-3   Q9BVC4-4   Q9BVC4-5   (Contains a N-acetylmethionine at position 1. Contains a phosphoserine at position 7)

Explore the universe of human proteins at neXtProt for MLST8: NX_Q9BVC4

Explore proteomics data for MLST8 at MOPED 

Post-translational modifications:

  • View modification sites using PhosphoSitePlus
  • View neXtProt modification sites for NX_Q9BVC4

  • MLST8 Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

    MLST8 Protein Expression
    REFSEQ proteins (4 alternative transcripts): 
    NP_001186102.1  NP_001186103.1  NP_001186104.1  NP_071767.3  

    ENSEMBL proteins: 
     ENSP00000455642   ENSP00000454698   ENSP00000457870   ENSP00000371888   ENSP00000456405  
     ENSP00000454629   ENSP00000456063   ENSP00000455850   ENSP00000456944   ENSP00000456046  
     ENSP00000455475   ENSP00000454235   ENSP00000459612   ENSP00000456076   ENSP00000301724  
     ENSP00000455458   ENSP00000380313   ENSP00000455046   ENSP00000301725  
    Reactome Protein details: Q9BVC4
    Human Recombinant Protein Products for MLST8: 
    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for MLST8
    OriGene Custom MassSpec 
    OriGene Custom Protein Services for MLST8
    GenScript Custom Purified and Recombinant Proteins Services for MLST8
    Novus Biologicals MLST8 Proteins
    Novus Biologicals MLST8 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates 
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp. 

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm ISS--
    GO:0005829cytosol TAS--

    MLST8 for ontologies           About GeneDecksing



    MLST8 Antibody Products: 
    EMD Millipore Mono- and Polyclonal Antibodies for the study of MLST8
    R&D Systems Antibodies for MLST8 (GBL)
    Cell Signaling Technology (CST) Antibodies for MLST8  (LST8)
    OriGene Antibodies for MLST8
    OriGene Custom Antibody Services for MLST8
    GenScript Custom Superior Antibodies Services for MLST8
    Novus Biologicals MLST8 Antibodies
    Abcam antibodies for MLST8
    Browse Antibodies at Cloud-Clone Corp. 
    ThermoFisher Antibodies for MLST8
    LSBio Antibodies in human, mouse, rat for MLST8 

    Assay Products for MLST8: 
    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for MLST8
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for MLST8
    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp. 
    Browse CLIAs at Cloud-Clone Corp.


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    HGNC Gene Families: 
    WDR: WD repeat domain containing

    5 InterPro protein domains:
     IPR019775 WD40_repeat_CS
     IPR001680 WD40_repeat
     IPR020472 G-protein_beta_WD-40_rep
     IPR017986 WD40_repeat_dom
     IPR015943 WD40/YVTN_repeat-like_dom

    Graphical View of Domain Structure for InterPro Entry Q9BVC4

    ProtoNet protein and cluster: Q9BVC4

    UniProtKB/Swiss-Prot: LST8_HUMAN, Q9BVC4
    Similarity: Belongs to the WD repeat LST8 family
    Similarity: Contains 7 WD repeats


    MLST8 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: LST8_HUMAN, Q9BVC4
    Function: Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and
    hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated
    mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the
    RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1
    requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated
    mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome
    synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E
    (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by
    phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and
    enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors
    RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be
    nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton,
    probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the
    serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473'
    phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1
    which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2
    also modulates the phosphorylation of PRKCA on 'Ser-657'

         Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI12408816
         
    MLST8 for ontologies           About GeneDecksing


    Phenotypes:
         5 MGI mutant phenotypes (inferred from 1 allele(MGI details for Mlst8):
     cardiovascular system  embryogenesis  growth/size  mortality/aging  nervous system 

    MLST8 for phenotypes           About GeneDecksing

    Animal Models:
       inGenious Targeting Laboratory - Custom generated mouse model solutions for MLST8 
       inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for MLST8

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for MLST8 
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for MLST8 

    miRNA
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    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat MLST8
    1 QIAGEN miScript miRNA Assays for microRNA that regulate MLST8:
    hsa-miR-2117
    SwitchGear 3'UTR luciferase reporter plasmidMLST8 3' UTR sequence
    Inhib. RNA
    Products:
        
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for MLST8
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    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for MLST8
    Sirion Biotech Customized adenovirus for overexpression of MLST8

    Clone
    Products:
         
    Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene clones in human, mouse for MLST8 (see all 7)
    OriGene ORF clones in mouse, rat for MLST8
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 4): MLST8 (NM_001199174)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MLST8
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MLST8
    Sirion Biotech Customized lentivirus for stable overexpression of MLST8 
                         Customized lentivirus expression plasmids for stable overexpression of MLST8 

    Cell Line
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    GenScript Custom overexpressing Cell Line Services for MLST8
    Search LifeMap BioReagents cell lines for MLST8
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MLST8


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
    About This Section

    SuperPaths for MLST8 About   (see all 23)                                                                                              See pathways by source

    SuperPathSelected contained pathways About (see all per SuperPath)
    1Energy dependent regulation of mTOR by LKB1-AMPK
    mTOR signalling0.63
    TOR signaling0.33
    PKB-mediated events0.61
    2Signaling by FGFR
    Downstream signaling of activated FGFR0.92
    Downstream signal transduction0.90
    Signaling by FGFR0.92
    Signaling by PDGF0.85
    Signaling by FGFR in disease0.91
    Signaling by EGFR0.84
    DAP12 signaling0.91
    Signaling by EGFR in Cancer0.83
    3PIP3 activates AKT signaling
    GAB1 signalosome0.93
    PI3K events in ERBB2 signaling0.88
    PI3K/AKT activation0.93
    PI3K events in ERBB4 signaling0.88
    PIP3 activates AKT signaling0.88
    Constitutive PI3K/AKT Signaling in Cancer0.88
    PI-3K cascade0.88
    Signaling by SCF-KIT0.72
    4IRS-related events
    Insulin receptor signalling cascade0.94
    IGF1R signaling cascade0.92
    IRS-related events0.94
    Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)0.92
    IRS-related events triggered by IGF1R0.93
    PI3K Cascade0.86
    IRS-mediated signalling0.92
    Signaling by Insulin receptor0.79
    5mTORC1-mediated signalling
    mTORC1-mediated signalling0.82
    Release of eIF4E0.55
    S6K1-mediated signalling0.82

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 R&D Systems Pathway for MLST8
        Akt Signaling Pathway


    1 Cell Signaling Technology (CST) Pathway for MLST8
        Translational Control

    5/9 BioSystems Pathways for MLST8 (see all 9)
        TOR signaling
    Senescence and Autophagy
    mTOR signaling pathway
    CXCR3-mediated signaling events
    Class I PI3K signaling events mediated by Akt

    5/47        Reactome Pathways for MLST8 (see all 47)
        Costimulation by the CD28 family
    Signaling by EGFR in Cancer
    Downstream signal transduction
    mTORC1-mediated signalling
    IRS-mediated signalling


    2         Kegg Pathways  (Kegg details for MLST8):
        mTOR signaling pathway
    PI3K-Akt signaling pathway


    MLST8 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for MLST8

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/422 Interacting proteins for MLST8 (Q9BVC41, 3 ENSP000003718884) via UniProtKB, MINT, STRING, and/or I2D (see all 422)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4AP628053I2D: score=1 
    HIST1H4BP628053I2D: score=1 
    HIST1H4CP628053I2D: score=1 
    HIST1H4DP628053I2D: score=1 
    HIST1H4EP628053I2D: score=1 
    About this table

    Gene Ontology (GO): 5/13 biological process terms (GO ID links to tree view) (see all 13):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007173epidermal growth factor receptor signaling pathway TAS--
    GO:0008286insulin receptor signaling pathway TAS--
    GO:0008543fibroblast growth factor receptor signaling pathway TAS--
    GO:0030838positive regulation of actin filament polymerization IEA--
    GO:0031295T cell costimulation TAS--

    MLST8 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    MLST8 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for MLST8 (LST8)

    6 Novoseek inferred chemical compound relationships for MLST8 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    adenylate 30.1 1 8670119 (1)
    pge1 23.5 1 2170366 (1)
    isoproterenol 19.4 1 9338092 (1)
    calcium 15.8 7 14507926 (2), 12221094 (1), 10970423 (1), 9355757 (1) (see all 6)
    atp 0 1 12512701 (1)
    dopamine 0 2 12043849 (1), 10909424 (1)

    Search CenterWatch for drugs/clinical trials and news about MLST8 / LST8

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for MLST8 gene (4 alternative transcripts): 
    NM_001199173.1  NM_001199174.1  NM_001199175.1  NM_022372.4  

    Unigene Cluster for MLST8:

    MTOR associated protein, LST8 homolog (S. cerevisiae)
    Hs.29203  [show with all ESTs]
    Unigene Representative Sequence: BC052292
    18/36 Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 36):
    ENST00000562043 ENST00000563179 ENST00000565926 ENST00000569457 ENST00000564088(uc002coy.3 uc002cpc.3)
    ENST00000382450 ENST00000569417(uc002cpb.3 uc002coz.3 uc010uvx.2)
    ENST00000570224 ENST00000568542(uc002cpd.3) ENST00000564294(uc010uvy.2)
    ENST00000562851 ENST00000567623 ENST00000562352 ENST00000565330 ENST00000567928
    ENST00000562479 ENST00000562844 ENST00000565269

    miRNA
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    Inhib. RNA
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    GenScript: all cDNA clones in your preferred vector (see all 4): MLST8 (NM_001199174)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MLST8
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MLST8
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat MLST8
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MLST8

    Additional mRNA sequence: 

    AF195883.1 AK021536.1 AK022227.1 AK098762.1 AK302201.1 AK304390.1 AY223837.1 BC001313.2 
    BC017119.1 BC052292.1 BC088354.1 

    24/30 DOTS entries (see all 30):

    DT.447261  DT.100842882  DT.95268698  DT.100803442  DT.95268700  DT.100842880  DT.100842886  DT.100842889 
    DT.91771751  DT.92020928  DT.100842881  DT.91771766  DT.120681470  DT.100772914  DT.120681448  DT.100842878 
    DT.100842896  DT.95268696  DT.100842869  DT.70103990  DT.91771764  DT.92016464  DT.100767237  DT.100842883 

    24/393 AceView cDNA sequences (see all 393):

    CR613836 AW072647 BC052292 BI766587 BP343198 BQ055743 BQ072489 BI909315 
    BI820282 AA279343 CR605614 CR610797 BM547247 BU730920 BM800847 CR591683 
    CR605693 AI380203 BQ877791 BM552443 BE260143 BI913217 BE886359 BX457638 

    GeneLoc Exon Structure

    5/21 Alternative Splicing Database (ASD) splice patterns (SP) for MLST8 (see all 21)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2a · 2b · 2c ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c · 5d · 5e ^ 6 ^ 7a · 7b ^ 8a · 8b · 8c · 8d ^ 9 ^ 10a · 10b · 10c ^ 11a ·
    SP1:                    -     -     -                       -                             -                                         -                 -         
    SP2:                                                        -                             -                                         -                 -         
    SP3:                    -     -     -     -                 -                             -                                         -                 -         
    SP4:                    -     -     -                       -                                                                                                   
    SP5:                    -     -     -                       -                             -                                         -     -     -     -         

    ExUns: 11b · 11c · 11d ^ 12a · 12b
    SP1:                              
    SP2:                              
    SP3:                              
    SP4:                              
    SP5:                              


    ECgene alternative splicing isoforms for MLST8

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    MLST8 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GCCAGGTGGA
    MLST8 Expression
    About this image


    See MLST8 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for MLST8

    SOURCE GeneReport for Unigene cluster: Hs.29203

    UniProtKB/Swiss-Prot: LST8_HUMAN, Q9BVC4
    Tissue specificity: Broadly expressed, with highest levels in skeletal muscle, heart and kidney

        SABiosciences Expression via Pathway-Focused PCR Array including MLST8: 
              mTOR Signaling in human mouse rat

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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MLST8

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for MLST8 gene from 10/25 species (see all 25)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Mlst81 , 5 MTOR associated protein, LST8 homolog (S. cerevisiae)1, 5 87.63(n)1
    97.85(a)1
      17 (12.39 cM)5
    567161  NM_001252463.11  NP_001239392.11 
     244735505 
    chicken
    (Gallus gallus)
    Aves MLST81 MTOR associated protein, LST8 homolog (S. cerevisiae) 84.62(n)
    93.85(a)
      416558  XM_414858.3  XP_414858.3 
    lizard
    (Anolis carolinensis)
    Reptilia MLST86
    Uncharacterized protein
    79(a)
    1 ↔ 1
    GL343827.1(23923-31257)
    African clawed frog
    (Xenopus laevis)
    Amphibia MGC687132 hypothetical protein MGC68713 79.46(n)    BC060429.1 
    zebrafish
    (Danio rerio)
    Actinopterygii BC044176.12   -- 80.17(n)   334244  BC044176.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG30041 CG3004 58.25(n)
    50.82(a)
      31903  NM_132344.3  NP_572572.1 
    worm
    (Caenorhabditis elegans)
    Secernentea C10H11.86
    Protein C10H11.8, isoform b
    22(a)
    1 ↔ 1
    I(4735756-4738100)
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes LST8(YNL006W)4
    LST81
    Protein required for the transport of amino acid permease more4
    Lst8p1
    50.45(n)1
    49.66(a)1
      14(620067-620978)4
    8557261, 4  NP_014392.31  NP_014392.14 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AT3G181401 transducin/WD40 domain-containing protein 51.62(n)
    47.16(a)
      821339  NM_112696.2  NP_188442.1 
    rice
    (Oryza sativa)
    Liliopsida Os03g06817001 hypothetical protein 52.73(n)
    49.83(a)
      4333729  NM_001057442.1  NP_001050907.1 


    ENSEMBL Gene Tree for MLST8 (if available)
    TreeFam Gene Tree for MLST8 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for MLST8 gene
    1 SIMAP similar gene for MLST8 using alignment to 10 protein entries:     LST8_HUMAN (see all proteins):
    KATNB1

    MLST8 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/170 SNPs in MLST8 are shown (see all 170)    About this table     
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 16 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs601549711,2
    C--2253300(+) ATAAA-/TAAA  
            
    AGGCC
    3 -- us2k11Minor allele frequency- TAAA:0.00NA 2
    rs781155281,2
    C,F--2253614(+) TGGACC/TCTGCG 3 -- us2k12Minor allele frequency- T:0.05CSA WA 120
    rs1873551891,2
    C--2253619(+) CCTGCA/GGGGCA 3 -- us2k10--------
    rs268651,2
    C,F,A,H--2253773(-) GCCCTT/CCAGCA 4 -- us2k19Minor allele frequency- C:0.46NS EA NA 660
    rs1924948231,2
    --2253853(+) TCAGGA/CATGGT 4 -- us2k10--------
    rs1391197051,2
    --2253862(+) GTGCCA/GTCAAC 4 -- us2k10--------
    rs133381401,2
    C,F--2253906(+) CCGGGC/TGCAGT 4 -- us2k14Minor allele frequency- T:0.29NA WA CSA 7
    rs268641,2
    C,F--2253918(-) caggcG/Atgagc 4 -- us2k14Minor allele frequency- A:0.38NA CSA 8
    rs1838272321,2
    --2253941(+) TTTGGG/TAGGCT 4 -- us2k10--------
    rs757995811,2
    C--2254016(+) AGTCTC/GTCTCT 4 -- us2k10--------

    HapMap Linkage Disequilibrium report for MLST8 (2254249 - 2259418 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 10/18 variations for MLST8 (see all 18):    About this table     
    Variant IDTypeSubtypePubMed ID
    esv2422427CNV Duplication17116639
    nsv1711CNV Insertion18451855
    dgv2566n71CNV Loss21882294
    dgv2562n71CNV Loss21882294
    dgv2545n71CNV Loss21882294
    dgv2546n71CNV Loss21882294
    nsv457351CNV Loss19166990
    dgv2540n71CNV Loss21882294
    nsv471072CNV Loss18288195
    nsv905132CNV Loss21882294

    1 SABiosciences Cancer Mutation PCR Assay for MLST8:
    Cosmic IdAA Change
    86101p.Q312R
    SeqTarget long-range PCR primers for resequencing MLST8
    DNA2.0 Custom Variant and Variant Library Synthesis for MLST8

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    OMIM gene information: 612190    OMIM disorders: --

    16 diseases for MLST8:    About MalaCards
    mental disorders    human t-cell leukemia virus type 1    tuberous sclerosis    cholera
    schizophrenia    bipolar disorder    t-cell leukemia    neuroblastoma
    colon cancer    alzheimer's disease    retinitis    endotheliitis
    leukemia    pancreatitis    hepatitis    neuronitis


    MLST8 for disorders           About GeneDecksing


    Congresses - knowledge worth sharing:  
    Alzheimer's & Parkinson's Diseases Congress (ADPD) 18 - 22 March 2015

    1 Novoseek inferred disease relationship for MLST8 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    schizophrenia 0 2 12043849 (1)

    Human Genome Epidemiology (HuGE) Navigator: MLST8 (1 document)

    Export disorders for MLST8 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for MLST8 gene, integrated from 9 sources (see all 131):
    (articles sorted by number of sources associating them with MLST8)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification of Protor as a novel Rictor-binding component of mTOR complex-2. (PubMed id 17461779)1, 2 Pearce L.R.... Alessi D.R. (2007)
    2. Rheb binds and regulates the mTOR kinase. (PubMed id 15854902)1, 2 Long X....Avruch J. (2005)
    3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    4. Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive. (PubMed id 15467718)1, 2 Jacinto E....Hall M.N. (2004)
    5. GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR. (PubMed id 12718876)1, 2 Kim D.-H.... Sabatini D.M. (2003)
    6. Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. (PubMed id 12408816)1, 2 Loewith R.... Hall M.N. (2002)
    7. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1, 3 Strausberg R.L....Marra M.A. (2002)
    8. A Y2H-seq approach defines the human protein methyltransferase interactome. (PubMed id 23455924)1 Weimann M.... Stelzl U. (2013)
    9. Association of CAD, a multifunctional protein involved in pyrimidine synthesis, with mLST8, a component of the mTOR complexes. (PubMed id 23594158)1 Nakashima A....Yonezawa K. (2013)
    10. Development and application of a DNA microarray-based yeast two-hybrid system. (PubMed id 23275563)1 Suter B....Wanker E.E. (2013)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 64223 HGNC: 24825 AceView: GBL Ensembl:ENSG00000167965 euGenes: HUgn64223
    ECgene: MLST8 Kegg: 64223 H-InvDB: MLST8

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for MLST8 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for MLST8 gene:
    Search GeneIP for patents involving MLST8

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Sirion Biotech, Cell lines from GenScript, and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
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    About This Section

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    GeneCards Homepage - Last full update: 23 Oct 2013 - Incrementals: 4 Nov 2013 , 7 Nov 2013 , 23 Jan 2014

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