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MLST8 Gene

protein-coding   GIFtS: 58
GCID: GC16P002254

MTOR Associated Protein, LST8 Homolog (S. Cerevisiae)

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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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This gene clusters with an RNA gene
Subcategory (RNA class): antisense

Quality score for the ORGUL clustered with this gene is 3

Aliases
MTOR Associated Protein, LST8 Homolog (S. Cerevisiae)1 2     G Protein Beta Subunit Like1
GBL2 3 5     GbetaL2
LST82 3 5     gable2
Mammalian Lethal With SEC13 Protein 82 3     Target Of Rapamycin Complex Subunit LST82
Protein GbetaL2 3     Gable3
TORC Subunit LST82 3     G Protein Beta Subunit-Like3
POP32 5     mLST83
WAT12 5     

External Ids:    HGNC: 248251   Entrez Gene: 642232   Ensembl: ENSG000001679657   OMIM: 6121905   UniProtKB: Q9BVC43   
ORGUL members:         

Export aliases for MLST8 gene to outside databases

Previous GC identifers: GC16P002196 GC16P002255 GC16P002182


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for MLST8 Gene:
MLST8 (MTOR associated protein, LST8 homolog (S. cerevisiae)) is a protein-coding gene.

UniProtKB/Swiss-Prot: LST8_HUMAN, Q9BVC4
Function: Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and
hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated
mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the
RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1
requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated
mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome
synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E
(eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by
phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and
enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors
RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be
nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton,
probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the
serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473'
phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1
which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2
also modulates the phosphorylation of PRKCA on 'Ser-657'

Gene Wiki entry for MLST8 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000016.9  NT_010393.17  NC_018927.2  
Regulatory elements:
   Search for regulatory transcription factor binding sites for MLST8
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): MLST8 promoter sequence
   Search Chromatin IP Primers for MLST8

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MLST8


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16p13.3   Ensembl cytogenetic band:  16p13.3   HGNC cytogenetic band: 16p13.3

MLST8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MLST8 gene location

GeneLoc information about chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16P002254:  view genomic region     (about GC identifiers)

Start:
2,254,249 bp from pter      End:
2,259,418 bp from pter
Size:
5,170 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: LST8_HUMAN, Q9BVC4 (See protein sequence)
Recommended Name: Target of rapamycin complex subunit LST8  
Size: 326 amino acids; 35876 Da
Subunit: Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR,
AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Part of the mammalian target of
rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1,
mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Interacts directly with MTOR and RPTOR.
Interacts with RHEB
Sequence caution: Sequence=EAW85539.1; Type=Erroneous gene model prediction;
6 PDB 3D structures from and Proteopedia for MLST8:
4JSN (3D)        4JSP (3D)        4JSV (3D)        4JSX (3D)        4JT5 (3D)        4JT6 (3D)    
Secondary accessions: B3KMM4 B4DY00 D3DU88 Q5M800 Q86Y18 Q8WUI5 Q9HA66 Q9UJV6
Alternative splicing: 4 isoforms:  Q9BVC4-1   Q9BVC4-3   Q9BVC4-4   Q9BVC4-5   (Contains a N-acetylmethionine at position 1. Contains a phosphoserine at position 7)

Explore the universe of human proteins at neXtProt for MLST8: NX_Q9BVC4

Explore proteomics data for MLST8 at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys86, Lys245, Lys305
  • Modification sites at PhosphoSitePlus

  • See MLST8 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (4 alternative transcripts): 
    NP_001186102.1  NP_001186103.1  NP_001186104.1  NP_071767.3  

    ENSEMBL proteins: 
     ENSP00000455642   ENSP00000454698   ENSP00000457870   ENSP00000371888   ENSP00000456405  
     ENSP00000454629   ENSP00000456063   ENSP00000455850   ENSP00000456944   ENSP00000456046  
     ENSP00000455475   ENSP00000454235   ENSP00000459612   ENSP00000456076   ENSP00000301724  
     ENSP00000455458   ENSP00000380313   ENSP00000455046   ENSP00000301725  
    Reactome Protein details: Q9BVC4

    MLST8 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for MLST8
    OriGene Custom MassSpec
    OriGene Custom Protein Services for MLST8
    GenScript Custom Purified and Recombinant Proteins Services for MLST8
    Novus Biologicals MLST8 Proteins
    Novus Biologicals MLST8 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp.

     
    Search eBioscience for Proteins for MLST8 

    MLST8 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of MLST8
    R&D Systems Antibodies for MLST8 (GBL)
    Cell Signaling Technology (CST) Antibodies for MLST8  (LST8)
    OriGene Antibodies for MLST8
    OriGene Custom Antibody Services for MLST8
    Novus Biologicals MLST8 Antibodies
    Abcam antibodies for MLST8
    Browse Antibodies at Cloud-Clone Corp.
    ThermoFisher Antibodies for MLST8
    LSBio Antibodies in human, mouse, rat for MLST8

    MLST8 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for MLST8
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for MLST8
    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp.
    Browse CLIAs at Cloud-Clone Corp.
    Search eBioscience for ELISAs for MLST8 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    WDR: WD repeat domain containing

    Selected InterPro protein domains (see all 6):
     IPR019775 WD40_repeat_CS
     IPR011047 Quinonprotein_ADH-like_supfam
     IPR001680 WD40_repeat
     IPR020472 G-protein_beta_WD-40_rep
     IPR017986 WD40_repeat_dom

    Graphical View of Domain Structure for InterPro Entry Q9BVC4

    ProtoNet protein and cluster: Q9BVC4

    UniProtKB/Swiss-Prot: LST8_HUMAN, Q9BVC4
    Similarity: Belongs to the WD repeat LST8 family
    Similarity: Contains 7 WD repeats


    Find genes that share domains with MLST8           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: LST8_HUMAN, Q9BVC4
    Function: Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and
    hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated
    mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the
    RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1
    requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated
    mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome
    synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E
    (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by
    phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and
    enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors
    RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be
    nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton,
    probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the
    serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473'
    phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1
    which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2
    also modulates the phosphorylation of PRKCA on 'Ser-657'

         Gene Ontology (GO): 1 molecular function term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI12408816
         
    Find genes that share ontologies with MLST8           About GenesLikeMe


    Phenotypes:
         5 MGI mutant phenotypes (inferred from 1 allele(MGI details for Mlst8):
     cardiovascular system  embryogenesis  growth/size/body  mortality/aging  nervous system 

    Find genes that share phenotypes with MLST8           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MLST8
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for MLST8

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for MLST8
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for MLST8

    miRNA
    Products:
        
    miRTarBase miRNAs that target MLST8:
    hsa-mir-4326 (MIRT052837)

    Block miRNA regulation of human, mouse, rat MLST8 using miScript Target Protectors
    1 qRT-PCR Assays for microRNA that regulate MLST8:
    hsa-miR-2117
    SwitchGear 3'UTR luciferase reporter plasmidMLST8 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for MLST8
    Predesigned siRNA for gene silencing in human, mouse, rat MLST8

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for MLST8

    Clone
    Products:
         
    OriGene clones in human, mouse for MLST8 (see all 7)
    OriGene ORF clones in mouse, rat for MLST8
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 4): MLST8 (NM_001199174)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MLST8
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MLST8
    Addgene plasmids for MLST8 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for MLST8
    Browse ESI BIO Cell Lines and PureStem Progenitors for MLST8 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MLST8


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    LST8_HUMAN, Q9BVC4: Cytoplasm (By similarity)
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol4
    cytoskeleton2
    extracellular1
    nucleus1

    Gene Ontology (GO): 2 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm ISS--
    GO:0005829cytosol TAS--

    Find genes that share ontologies with MLST8           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for MLST8 About   (see all 22)  
    See pathways by source

    SuperPathSelected contained pathways About (see all per SuperPath)
    1Insulin receptor signalling cascade
    Insulin receptor signalling cascade0.94
    Signaling by Insulin receptor0.80
    IRS-related events0.94
    PKB-mediated events0.41
    Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)0.92
    mTOR signalling0.39
    IRS-mediated signalling0.92
    TOR signaling0.32
    2PI-3K cascade
    PI3K/AKT activation0.93
    PI3K/AKT Signaling in Cancer0.88
    GAB1 signalosome0.93
    PI3K events in ERBB4 signaling0.88
    Constitutive PI3K/AKT Signaling in Cancer0.88
    Role of LAT2/NTAL/LAB on calcium mobilization0.80
    PIP3 activates AKT signaling0.88
    Signaling by SCF-KIT0.72
    3Signaling by FGFR
    Signaling by FGFR0.91
    DAP12 interactions0.89
    Signaling by FGFR in disease0.91
    Signaling by PDGF0.87
    Downstream signaling of activated FGFR0.91
    Signaling by EGFR0.83
    Downstream signal transduction0.90
    Signaling by EGFR in Cancer0.82
    4CD28 co-stimulation
    CD28 co-stimulation0.66
    Costimulation by the CD28 family0.44
    CD28 dependent PI3K/Akt signaling0.66
    5Downstream Signaling Events Of B Cell Receptor (BCR)
    Downstream Signaling Events Of B Cell Receptor (BCR)0.79
    Signaling by the B Cell Receptor (BCR)0.79


    Find genes that share SuperPaths with MLST8           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 R&D Systems Pathway for MLST8
        Akt Signaling Pathway

    1 Downloadable PowerPoint Slide of GeneGlobe Pathway Central Maps for MLST8
        mTOR Pathway

    1 Cell Signaling Technology (CST) Pathway for MLST8
        Translational Control

    Selected BioSystems Pathways for MLST8 (see all 11)
        TOR signaling
    Senescence and Autophagy
    Interferon type I
    Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
    mTOR signaling pathway

    Selected Reactome Pathways for MLST8 (see all 7)
        Release of eIF4E
    HSF1-dependent transactivation
    Constitutive PI3K/AKT Signaling in Cancer
    S6K1-mediated signalling
    PIP3 activates AKT signaling


    2 Kegg Pathways  (Kegg details for MLST8):
        mTOR signaling pathway
    PI3K-Akt signaling pathway

        Pathway & Disease-focused RT2 Profiler PCR Array including MLST8: 
              mTOR Signaling in human mouse rat

    Interactions:

        Search GeneGlobe Interaction Network for MLST8

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for MLST8 (Q9BVC41, 3 ENSP000003718884) via UniProtKB, MINT, STRING, and/or I2D (see all 501)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4AP628053I2D: score=1 
    HIST1H4BP628053I2D: score=1 
    HIST1H4CP628053I2D: score=1 
    HIST1H4DP628053I2D: score=1 
    HIST1H4EP628053I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 13):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007173epidermal growth factor receptor signaling pathway TAS--
    GO:0008286insulin receptor signaling pathway TAS--
    GO:0008543fibroblast growth factor receptor signaling pathway TAS--
    GO:0030838positive regulation of actin filament polymerization IEA--
    GO:0031295T cell costimulation TAS--

    Find genes that share ontologies with MLST8           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for MLST8 (LST8)

    6 Novoseek inferred chemical compound relationships for MLST8 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    adenylate 30.1 1 8670119 (1)
    pge1 23.5 1 2170366 (1)
    isoproterenol 19.4 1 9338092 (1)
    calcium 15.8 7 14507926 (2), 12221094 (1), 10970423 (1), 9355757 (1) (see all 6)
    atp 0 1 12512701 (1)
    dopamine 0 2 12043849 (1), 10909424 (1)



    Find genes that share compounds with MLST8           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

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    REFSEQ mRNAs for MLST8 gene (4 alternative transcripts): 
    NM_001199173.1  NM_001199174.1  NM_001199175.1  NM_022372.4  

    Unigene Cluster for MLST8:

    MTOR associated protein, LST8 homolog (S. cerevisiae)
    Hs.29203  [show with all ESTs]
    Unigene Representative Sequence: BC052292
    Selected Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 36):
    ENST00000562043 ENST00000563179 ENST00000565926 ENST00000569457 ENST00000564088(uc002coy.3 uc002cpc.3)
    ENST00000382450 ENST00000569417(uc002cpb.3 uc002coz.3 uc010uvx.2)
    ENST00000570224 ENST00000568542(uc002cpd.3) ENST00000564294(uc010uvy.2)
    ENST00000562851 ENST00000567623 ENST00000562352 ENST00000565330 ENST00000567928
    ENST00000562479 ENST00000562844 ENST00000565269
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat MLST8 using miScript Target Protectors
    1 qRT-PCR Assays for microRNA that regulate MLST8:
    hsa-miR-2117
    SwitchGear 3'UTR luciferase reporter plasmidMLST8 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for MLST8
    Predesigned siRNA for gene silencing in human, mouse, rat MLST8
    Clone
    Products:
         
    OriGene clones in human, mouse for MLST8 (see all 7)
    OriGene ORF clones in mouse, rat for MLST8
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 4): MLST8 (NM_001199174)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MLST8
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MLST8
    Addgene plasmids for MLST8 
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for MLST8
    OriGene qSTAR qPCR primer pairs in human, mouse for MLST8
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MLST8
      QuantiTect SYBR Green Assays in human, mouse, rat MLST8
      QuantiFast Probe-based Assays in human, mouse, rat MLST8

    Additional mRNA sequence: 

    AF195883.1 AK021536.1 AK022227.1 AK098762.1 AK302201.1 AK304390.1 AY223837.1 BC001313.2 
    BC017119.1 BC052292.1 BC088354.1 

    Selected DOTS entries (see all 30):

    DT.447261  DT.100842882  DT.95268698  DT.100803442  DT.95268700  DT.100842880  DT.100842886  DT.100842889 
    DT.91771751  DT.92020928  DT.100842881  DT.91771766  DT.120681470  DT.100772914  DT.120681448  DT.100842878 
    DT.100842896  DT.95268696  DT.100842869  DT.70103990  DT.91771764  DT.92016464  DT.100767237  DT.100842883 

    Selected AceView cDNA sequences (see all 393):

    CR596002 BU167228 CR610557 BP338038 BU730920 CR613836 BM552443 BQ064206 
    CA488312 BQ636116 BI820282 BC001313 BG291182 CR599293 CR596334 H61681 
    CR602887 BC017119 BE886359 BU625761 BM921985 AI989687 BU845488 BM759574 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for MLST8 (see all 21)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2a · 2b · 2c ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c · 5d · 5e ^ 6 ^ 7a · 7b ^ 8a · 8b · 8c · 8d ^ 9 ^ 10a · 10b · 10c ^ 11a ·
    SP1:                    -     -     -                       -                             -                                         -                 -         
    SP2:                                                        -                             -                                         -                 -         
    SP3:                    -     -     -     -                 -                             -                                         -                 -         
    SP4:                    -     -     -                       -                                                                                                   
    SP5:                    -     -     -                       -                             -                                         -     -     -     -         

    ExUns: 11b · 11c · 11d ^ 12a · 12b
    SP1:                              
    SP2:                              
    SP3:                              
    SP4:                              
    SP5:                              


    ECgene alternative splicing isoforms for MLST8

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    MLST8 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GCCAGGTGGA
    MLST8 Expression
    About this image

    MLST8 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    MLST8 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.29203

    UniProtKB/Swiss-Prot: LST8_HUMAN, Q9BVC4
    Tissue specificity: Broadly expressed, with highest levels in skeletal muscle, heart and kidney

        Pathway & Disease-focused RT2 Profiler PCR Array including MLST8: 
              mTOR Signaling in human mouse rat

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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MLST8

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for MLST8 gene from Selected species (see all 25)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Mlst81 , 5 MTOR associated protein, LST8 homolog (S. cerevisiae)1, 5 87.63(n)1
    97.85(a)1
      17 (12.39 cM)5
    567161  NM_001252463.11  NP_001239392.11 
     244735505 
    chicken
    (Gallus gallus)
    Aves MLST81 MTOR associated protein, LST8 homolog (S. cerevisiae) 84.66(n)
    93.87(a)
      416558  XM_004945247.1  XP_004945304.1 
    lizard
    (Anolis carolinensis)
    Reptilia MLST86
    MTOR associated protein, LST8 homolog (S. cerevisi...
    79(a)
    1 ↔ 1
    GL343827.1(23923-31257)
    African clawed frog
    (Xenopus laevis)
    Amphibia MGC687132 hypothetical protein MGC68713 79.46(n)    BC060429.1 
    zebrafish
    (Danio rerio)
    Actinopterygii BC044176.12   -- 80.17(n)   334244  BC044176.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Lst81 Lst8 58.25(n)
    50.82(a)
      31903  NM_132344.4  NP_572572.1 
    worm
    (Caenorhabditis elegans)
    Secernentea C10H11.86
    Protein C10H11.8, isoform b
    22(a)
    1 ↔ 1
    I(4735756-4738100) WBGene00015697
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes LST8(YNL006W)4
    LST81
    Protein required for the transport of amino acid permease more4
    LST81
    50.78(n)1
    49.66(a)1
      14(620067-620978)4
    8557261, 4  NP_014392.31  NP_014392.14 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AT3G181401 AT3G18140 51.39(n)
    47.16(a)
      821339  NM_112696.2  NP_188442.1 
    rice
    (Oryza sativa)
    Liliopsida Os03g06817001 Os03g0681700 52.51(n)
    49.83(a)
      4333729  NM_001057442.1  NP_001050907.1 


    ENSEMBL Gene Tree for MLST8 (if available)
    TreeFam Gene Tree for MLST8 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for MLST8 gene
    1 SIMAP similar gene for MLST8 using alignment to 10 protein entries:     LST8_HUMAN (see all proteins):
    KATNB1

    Find genes that share paralogs with MLST8           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for MLST8 (see all 170)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 16 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs601549711,2
    C--2253300(+) ATAAA-/TAAA  
            
    AGGCC
    3 -- us2k11Minor allele frequency- TAAA:0.00NA 2
    rs781155281,2
    C,F--2253614(+) TGGACC/TCTGCG 3 -- us2k12Minor allele frequency- T:0.05CSA WA 120
    rs1873551891,2
    C--2253619(+) CCTGCA/GGGGCA 3 -- us2k10--------
    rs268651,2
    C,F,A,H--2253773(-) GCCCTT/CCAGCA 4 -- us2k19Minor allele frequency- C:0.46NS EA NA 660
    rs1924948231,2
    --2253853(+) TCAGGA/CATGGT 4 -- us2k10--------
    rs1391197051,2
    --2253862(+) GTGCCA/GTCAAC 4 -- us2k10--------
    rs133381401,2
    C,F--2253906(+) CCGGGC/TGCAGT 4 -- us2k14Minor allele frequency- T:0.29NA WA CSA 7
    rs268641,2
    C,F--2253918(-) caggcG/Atgagc 4 -- us2k14Minor allele frequency- A:0.38NA CSA 8
    rs1838272321,2
    --2253941(+) TTTGGG/TAGGCT 4 -- us2k10--------
    rs757995811,2
    C--2254016(+) AGTCTC/GTCTCT 4 -- us2k10--------

    HapMap Linkage Disequilibrium report for MLST8 (2254249 - 2259418 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for MLST8 (see all 18):    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2422427CNV Duplication17116639
    nsv1711CNV Insertion18451855
    dgv2566n71CNV Loss21882294
    dgv2562n71CNV Loss21882294
    dgv2545n71CNV Loss21882294
    dgv2546n71CNV Loss21882294
    nsv457351CNV Loss19166990
    dgv2540n71CNV Loss21882294
    nsv471072CNV Loss18288195
    nsv905132CNV Loss21882294

    1 Site Specific Mutation Identification with PCR Assay for MLST8:
    Cosmic IdAA Change
    86101p.Q312R
    SeqTarget long-range PCR primers for resequencing MLST8
    DNA2.0 Custom Variant and Variant Library Synthesis for MLST8

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 612190    OMIM disorders: --


    Find genes that share disorders with MLST8           About GenesLikeMe

    1 Novoseek inferred disease relationship for MLST8 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    schizophrenia 0 2 12043849 (1)

    Human Genome Epidemiology (HuGE) Navigator: MLST8 (1 document)

    Export disorders for MLST8 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for MLST8 gene, integrated from 10 sources (see all 134):
    (articles sorted by number of sources associating them with MLST8)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification of Protor as a novel Rictor-binding component of mTOR complex-2. (PubMed id 17461779)1, 2 Pearce L.R.... Alessi D.R. (Biochem. J. 2007)
    2. Rheb binds and regulates the mTOR kinase. (PubMed id 15854902)1, 2 Long X.... Avruch J. (Curr. Biol. 2005)
    3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    4. Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive. (PubMed id 15467718)1, 2 Jacinto E....Hall M.N. (Nat. Cell Biol. 2004)
    5. GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR. (PubMed id 12718876)1, 2 Kim D.-H.... Sabatini D.M. (Mol. Cell 2003)
    6. Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. (PubMed id 12408816)1, 2 Loewith R.... Hall M.N. (Mol. Cell 2002)
    7. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1, 3 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)
    8. Pathway Analysis of ChIP-Seq-Based NRF1 Target Genes Suggests a Logical Hypothesis of their Involvement in the Pathogenesis of Neurodegenerative Diseases. (PubMed id 24250222)1 Satoh J....Yamamoto Y. (Gene Regul Syst Bio 2013)
    9. A Y2H-seq approach defines the human protein methyltransferase interactome. (PubMed id 23455924)1 Weimann M.... Stelzl U. (Nat. Methods 2013)
    10. Association of CAD, a multifunctional protein involved in pyrimidine synthesis, with mLST8, a component of the mTOR complexes. (PubMed id 23594158)1 Nakashima A....Yonezawa K. (J. Biomed. Sci. 2013)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 64223 HGNC: 24825 AceView: GBL Ensembl:ENSG00000167965 euGenes: HUgn64223
    ECgene: MLST8 Kegg: 64223 H-InvDB: MLST8

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for MLST8 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

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    Patent Information for MLST8 gene:
    Search GeneIP for patents involving MLST8

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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