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MLKL Gene

protein-coding   GIFtS: 57
GCID: GC16M074705

Mixed Lineage Kinase Domain-Like

  Search for MLKL
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Mixed Lineage Kinase Domain-Like1 2
hMLKL2 3
Mixed Lineage Kinase Domain-Like Protein2

External Ids:    HGNC: 266171   Entrez Gene: 1972592   Ensembl: ENSG000001684047   OMIM: 6151535   UniProtKB: Q8NB163   

Export aliases for MLKL gene to outside databases

Previous GC identifers: GC16M073264 GC16M060455


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for MLKL Gene:
MLKL (mixed lineage kinase domain-like) is a protein-coding gene. GO annotations related to this gene include protein kinase activity.

UniProtKB/Swiss-Prot: MLKL_HUMAN, Q8NB16
Function: Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process.
Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane
and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have
protein kinase activity




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000016.9  NT_010498.16  NC_018927.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the MLKL gene promoter:
         TBP   AML1a   POU6F1 (c2)   E4BP4   C/EBPalpha   Evi-1   CREB   NRF-2   deltaCREB   En-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMLKL promoter sequence
   Search Chromatin IP Primers for MLKL

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MLKL


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16q23.1   Ensembl cytogenetic band:  16q23.1   HGNC cytogenetic band: 16q22.3

MLKL Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MLKL gene location

GeneLoc information about chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16M074705:  view genomic region     (about GC identifiers)

Start:
74,705,753 bp from pter      End:
74,734,858 bp from pter
Size:
29,106 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: MLKL_HUMAN, Q8NB16 (See protein sequence)
Recommended Name: Mixed lineage kinase domain-like protein  
Size: 471 amino acids; 54479 Da
Subunit: Homotrimer; forms homotrimers on necroptosis induction. Interacts with RIPK3; the interaction is direct.
Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3. Within this complex, may play a role in
the proper targeting of RIPK1/RIPK3 to its downstream effector PGAM5
Miscellaneous: Interaction with RIPK3 is species specific: human MLKL only interacts with human RIPK3 and not
mouse RIPK3 (PubMed:22265413)
2 PDB 3D structures from and Proteopedia for MLKL:
4M67 (3D)        4MWI (3D)    
Secondary accessions: A6NCE4 Q8N6V0
Alternative splicing: 2 isoforms:  Q8NB16-1   Q8NB16-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for MLKL: NX_Q8NB16

Explore proteomics data for MLKL at MOPED

Post-translational modifications: 

  • Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces
    homotrimerization and localization to the plasma membrane1
  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See MLKL Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001135969.1  NP_689862.1  

    ENSEMBL proteins: 
     ENSP00000303118   ENSP00000308351   ENSP00000459935   ENSP00000461110   ENSP00000459920  
     ENSP00000461666  

    MLKL Human Recombinant Protein Products:

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    Browse OriGene Protein Over-expression Lysates
    OriGene Custom MassSpec
    OriGene Custom Protein Services for MLKL
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    Novus Biologicals MLKL Proteins
    Novus Biologicals MLKL Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for MLKL

     
    Search eBioscience for Proteins for MLKL 

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    Cloud-Clone Corp. ELISAs for MLKL
    Cloud-Clone Corp. CLIAs for MLKL
    Search eBioscience for ELISAs for MLKL 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    IUPHAR Guide to PHARMACOLOGY protein family classification: mixed lineage kinase domain-like
    TKL-unique family

    3 InterPro protein domains:
     IPR011009 Kinase-like_dom
     IPR000719 Prot_kinase_dom
     IPR001245 Ser-Thr/Tyr_kinase_cat_dom

    Graphical View of Domain Structure for InterPro Entry Q8NB16

    ProtoNet protein and cluster: Q8NB16

    UniProtKB/Swiss-Prot: MLKL_HUMAN, Q8NB16
    Domain: The protein kinase domain is catalytically inactive but contains an unusual pseudoactive site with an
    interaction between Lys-230 and Gln-356 residues. Upon phosphorylation by RIPK3, undergoes an active conformation
    (By similarity)
    Domain: The coiled coil region 2 is responsible for homotrimerization (PubMed:24316671)
    Similarity: Belongs to the protein kinase superfamily
    Similarity: Contains 1 protein kinase domain


    Find genes that share domains with MLKL           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: MLKL_HUMAN, Q8NB16
    Function: Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process.
    Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane
    and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have
    protein kinase activity
    Enzyme regulation: Inhibited by necrosulfonamide, a specific inhibitor of necroptosis that targets Cys-86

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004672NOT protein kinase activity ISS--
    GO:0004713protein tyrosine kinase activity ----
    GO:0005515protein binding IPI--
    GO:0005524ATP binding IDA--
    GO:0016772transferase activity, transferring phosphorus-containing groups ----
         
    Find genes that share ontologies with MLKL           About GenesLikeMe


    Phenotypes:
         6 GenomeRNAi human phenotypes for MLKL:
     Decreased focal adhesion (FA)   Decreased influenza A virus in  Downregulation of NF-kappaB pa  Downregulation of NF-kappaB pa 
     Increased colony dispersion (i  Synthetic lethal with Ras 

         5 MGI mutant phenotypes (inferred from 1 allele(MGI details for Mlkl):
     cellular  hematopoietic system  homeostasis/metabolism  immune system  mortality/aging 

    Find genes that share phenotypes with MLKL           About GenesLikeMe

    Animal Models:
         MGI mouse knock-out Mlklem1Jhan for MLKL

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MLKL
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for MLKL

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for MLKL
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for MLKL

    miRNA
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    Block miRNA regulation of human, mouse, rat MLKL using miScript Target Protectors
    4 qRT-PCR Assays for microRNAs that regulate MLKL:
    hsa-miR-3655 hsa-miR-194 hsa-miR-578 hsa-miR-103a-2*
    SwitchGear 3'UTR luciferase reporter plasmidMLKL 3' UTR sequence
    Inhib. RNA
    Products:
        
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    Predesigned siRNA for gene silencing in human, mouse, rat MLKL

    Gene Editing
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    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): MLKL (NM_152649)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MLKL
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MLKL

    Cell Line
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    Browse ESI BIO Cell Lines and PureStem Progenitors for MLKL 
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MLKL


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    MLKL_HUMAN, Q8NB16: Cytoplasm. Cell membrane. Note=Localizes to the cytoplasm and translocates to the plasma
    membrane on necroptosis induction
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol3
    nucleus2
    endoplasmic reticulum1
    mitochondrion1

    Gene Ontology (GO): 2 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IDA--
    GO:0005886plasma membrane IDA--

    Find genes that share ontologies with MLKL           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for MLKL About    
    See pathways by source

    SuperPathContained pathways About
    1Wnt Signaling Pathway (WikiPathways)
    DNA damage response (only ATM dependent)0.32
    2TNF signaling pathway
    TNF signaling pathway


    Find genes that share SuperPaths with MLKL           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 BioSystems Pathway for MLKL
        DNA damage response (only ATM dependent)


    1 Kegg Pathway  (Kegg details for MLKL):
        TNF signaling pathway

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for MLKL
    Interactions:

        Search GeneGlobe Interaction Network for MLKL

    STRING Interaction Network Preview (showing 5 interactants - click image to see 9)

    Selected Interacting proteins for MLKL (Q8NB161, 2, 3 ENSP000003083514) via UniProtKB, MINT, STRING, and/or I2D (see all 597)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ENSG00000237344P404252, 3, ENSP000003971774MINT-62574 MINT-62748 MINT-62672 I2D: score=2 STRING: ENSP00000397177
    ENSG00000206315P404252, 3MINT-62574 MINT-62748 MINT-62672 I2D: score=2 
    ENSG00000224952P404252, 3MINT-62574 MINT-62748 MINT-62672 I2D: score=2 
    ENSG00000225987P404252, 3MINT-62574 MINT-62748 MINT-62672 I2D: score=2 
    ENSG00000232005P404252, 3MINT-62574 MINT-62748 MINT-62672 I2D: score=2 
    About this table

    Gene Ontology (GO): 3 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006468protein phosphorylation ----
    GO:0070207protein homotrimerization IDA--
    GO:0070266necroptotic process IMP--

    Find genes that share ontologies with MLKL           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for MLKL

    2 HMDB Compounds for MLKL    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for MLKL gene (2 alternative transcripts): 
    NM_001142497.1  NM_152649.2  

    Unigene Cluster for MLKL:

    Mixed lineage kinase domain-like
    Hs.119878  [show with all ESTs]
    Unigene Representative Sequence: AK122998
    8 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000306247 ENST00000575695 ENST00000570846 ENST00000308807(uc002fdb.2 uc002fdc.2)
    ENST00000576529 ENST00000571303 ENST00000573267 ENST00000575686
    miRNA
    Products:
         
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    4 qRT-PCR Assays for microRNAs that regulate MLKL:
    hsa-miR-3655 hsa-miR-194 hsa-miR-578 hsa-miR-103a-2*
    SwitchGear 3'UTR luciferase reporter plasmidMLKL 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for MLKL
    Predesigned siRNA for gene silencing in human, mouse, rat MLKL
    Clone
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    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): MLKL (NM_152649)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MLKL
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MLKL
    Primer
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    OriGene qPCR primer pairs and template standards for MLKL
    OriGene qSTAR qPCR primer pairs in human, mouse for MLKL
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MLKL
      QuantiTect SYBR Green Assays in human, mouse, rat MLKL
      QuantiFast Probe-based Assays in human, mouse, rat MLKL

    Additional mRNA sequence: 

    AK091708.1 AK093013.1 AK122998.1 AL833362.1 BC028141.1 

    7 DOTS entries:

    DT.100649024  DT.409298  DT.100025407  DT.421346  DT.100649026  DT.101985719  DT.75157785 

    Selected AceView cDNA sequences (see all 64):

    BP345323 CR607857 AA706818 CA487239 CB159407 BC028141 BQ709249 CA487359 
    AL833362 CR595250 BQ575071 AA582672 AW963910 CB069503 BX390794 AI471283 
    AA381405 CF131680 AI334217 BX106138 AK091708 AL600710 AI351962 AA307123 

    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for MLKL    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b ^ 10 ^ 11
    SP1:                                      -     -                       -               
    SP2:                                      -                                             
    SP3:              -     -     -     -     -     -     -     -                           
    SP4:                                                                                    


    ECgene alternative splicing isoforms for MLKL

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    MLKL expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AATGAGGCCA
    MLKL Expression
    About this image


    MLKL expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 5) fully expand
     
     Heart (Cardiovascular System)
             Mature Cardiac Fibroblasts Myocardium
     
     Fibroblasts
             Mature Cardiac Fibroblasts Myocardium
     
     Testis (Reproductive System)
             Spermatid Seminiferous Tubules
     
     Gonad
             Spermatid Seminiferous Tubules
     
     Dermis (Integumentary System)
    MLKL Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    MLKL Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.119878
        Custom PCR Arrays for MLKL
    Primer
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    OriGene qPCR primer pairs and template standards for MLKL
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    QuantiFast Probe-based Assays in human, mouse, rat MLKL
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MLKL

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of chordates.

    Orthologs for MLKL gene from Selected species (see all 9)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Mlkl1 , 5 mixed lineage kinase domain-like1, 5 75.92(n)1
    63.66(a)1
      8 (57.98 cM)5
    745681  NM_029005.21  NP_083281.11 
     1113117975 
    chicken
    (Gallus gallus)
    Aves MLKL1 mixed lineage kinase domain-like 54.2(n)
    41.45(a)
      415686  XM_003641903.2  XP_003641951.1 
    lizard
    (Anolis carolinensis)
    Reptilia MLKL6
    mixed lineage kinase domain-like
    41(a)
    1 ↔ 1
    GL343840.1(169943-181785)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia mlkl1 mixed lineage kinase domain-like 48.97(n)
    37.67(a)
      100379899  XM_002931665.2  XP_002931711.1 


    ENSEMBL Gene Tree for MLKL (if available)
    TreeFam Gene Tree for MLKL (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for MLKL gene
    2 SIMAP similar genes for MLKL using alignment to 5 protein entries:     MLKL_HUMAN (see all proteins):
    DKFZp667E1714    TNNI3K

    Find genes that share paralogs with MLKL           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for MLKL (see all 844)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 16 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0413574
    A gastric adenocarcinoma sample4--see VAR_0413572 F I mis40--------
    VAR_0413554
    A gastric adenocarcinoma sample4--see VAR_0413552 L P mis40--------
    rs1860751011,2
    --74705277(+) CCTCCC/TGCCTG 2 -- int10--------
    rs1472176231,2
    --74705295(+) CAAAGC/TGCTGG 2 -- int10--------
    rs1406501341,2
    C--74705296(+) AAAGCA/GCTGGG 2 -- int10--------
    rs1427964841,2
    --74705321(+) TCACCA/GTGCCC 2 -- int10--------
    rs1474119331,2
    --74705352(+) GAGATA/GCTGAC 2 -- int10--------
    rs801476021,2
    C,F--74705424(+) GCTCTA/GGAAAT 2 -- int15Minor allele frequency- G:0.08NA WA EA 362
    rs1908814911,2
    C--74705458(+) AAAGTA/GTAGAC 2 -- int10--------
    rs1485267001,2
    --74705588(+) GAGAAC/TTCTGG 2 -- int10--------

    HapMap Linkage Disequilibrium report for MLKL (74705753 - 74734858 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for MLKL:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv906907CNV Loss21882294
    nsv906906CNV Gain21882294
    esv275138CNV Gain+Loss21479260

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing MLKL
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 615153    OMIM disorders: --


    Find genes that share disorders with MLKL           About GenesLikeMe

    Genetic Association Database (GAD): MLKL
    Human Genome Epidemiology (HuGE) Navigator: MLKL (1 document)

    Export disorders for MLKL gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for MLKL gene, integrated from 10 sources (see all 27):
    (articles sorted by number of sources associating them with MLKL)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Insights into the evolution of divergent nucleotide-binding mechanisms among pseudokinases revealed by crystal structures of human and mouse MLKL. (PubMed id 24219132)1, 2 Murphy J.M....Czabotar P.E. (Biochem. J. 2014)
    2. Plasma membrane translocation of trimerized MLKL protein is required for TNF-induced necroptosis. (PubMed id 24316671)1, 2 Cai Z.... Liu Z.G. (Nat. Cell Biol. 2014)
    3. Mixed lineage kinase domain-like is a key receptor interacting protein 3 downstream component of TNF-induced necrosis. (PubMed id 22421439)1, 2 Zhao J....Liu Z.G. (Proc. Natl. Acad. Sci. U.S.A. 2012)
    4. Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. (PubMed id 22265413)1, 2 Sun L....Wang X. (Cell 2012)
    5. Fine mapping and association studies of a high-density lipoprotein cholesterol linkage region on chromosome 16 in French-Canadian subjects. (PubMed id 19844255)1, 4 Dastani Z....Genest J. (Eur. J. Hum. Genet. 2010)
    6. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    7. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    8. The protein kinase complement of the human genome. (PubMed id 12471243)1, 2 Manning G.... Sudarsanam S. (Science 2002)
    9. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1, 3 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)
    10. Pronecrotic mixed lineage kinase domain-like protein expression is a prognostic biomarker in patients with early-stage resected pancreatic adenocarcinoma. (PubMed id 23720157)1 Colbert L.E....Yu D.S. (Cancer 2013)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 197259 HGNC: 26617 AceView: FLJ34389 Ensembl:ENSG00000168404 euGenes: HUgn197259
    ECgene: MLKL Kegg: 197259 H-InvDB: MLKL

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for MLKL Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for MLKL gene:
    Search GeneIP for patents involving MLKL

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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