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Aliases for MLK4 Gene

Aliases for MLK4 Gene

  • Mixed Lineage Kinase 4 3 4
  • Mitogen-Activated Protein Kinase Kinase Kinase MLK4 3 5
  • EC 2.7.11.25 4 61
  • KIAA1804 3 4
  • RP5-862P8.2 5
  • DJ862P8.3 3
  • EC 2.7.11 61

External Ids for MLK4 Gene

Summaries for MLK4 Gene

GeneCards Summary for MLK4 Gene

MLK4 (Mixed Lineage Kinase 4) is a Protein Coding gene. An important paralog of this gene is MAP3K9.

UniProtKB/Swiss-Prot for MLK4 Gene

  • Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MLK4 Gene

Genomics for MLK4 Gene

Regulatory Elements for MLK4 Gene

Enhancers for MLK4 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
- Elite enhancer/Elite enhancer-gene association

Enhancers around MLK4 on UCSC Golden Path with GeneCards custom track

Promoters for MLK4 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around MLK4 on UCSC Golden Path with GeneCards custom track

Genomic Location for MLK4 Gene

Chromosome:
1
Start:
233,327,743 bp from pter
End:
233,385,148 bp from pter
Size:
57,406 bases
Orientation:
Plus strand

Genomic View for MLK4 Gene

Genes around MLK4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MLK4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MLK4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MLK4 Gene

Proteins for MLK4 Gene

  • Protein details for MLK4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q5TCX8-M3KL4_HUMAN
    Recommended name:
    Mitogen-activated protein kinase kinase kinase MLK4
    Protein Accession:
    Q5TCX8
    Secondary Accessions:
    • B2RN34
    • Q5TCX7
    • Q5TCX9
    • Q8WWN1
    • Q8WWN2
    • Q96JM1

    Protein attributes for MLK4 Gene

    Size:
    1036 amino acids
    Molecular mass:
    113957 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Homodimer (By similarity). Interacts with TLR4.
    SequenceCaution:
    • Sequence=BAB47433.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for MLK4 Gene

    Alternative splice isoforms for MLK4 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MLK4 Gene

Selected DME Specific Peptides for MLK4 Gene

Post-translational modifications for MLK4 Gene

  • Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for MLK4 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for MLK4 Gene

Suggested Antigen Peptide Sequences for MLK4 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q5TCX8

UniProtKB/Swiss-Prot:

M3KL4_HUMAN :
  • Contains 1 protein kinase domain.
  • Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Domain:
  • Contains 1 protein kinase domain.
  • Contains 1 SH3 domain.
Family:
  • Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
genes like me logo Genes that share domains with MLK4: view

No data available for Gene Families for MLK4 Gene

Function for MLK4 Gene

Molecular function for MLK4 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.
UniProtKB/Swiss-Prot Function:
Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.

Enzyme Numbers (IUBMB) for MLK4 Gene

Gene Ontology (GO) - Molecular Function for MLK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004709 MAP kinase kinase kinase activity IEA --
GO:0005515 protein binding IPI 26859459
GO:0005524 ATP binding IEA --
GO:0042803 protein homodimerization activity ISS --
genes like me logo Genes that share ontologies with MLK4: view
genes like me logo Genes that share phenotypes with MLK4: view

Animal Model Products

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for MLK4 Gene

Localization for MLK4 Gene

Gene Ontology (GO) - Cellular Components for MLK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005575 cellular_component ND --
GO:0005622 intracellular IEA --
genes like me logo Genes that share ontologies with MLK4: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot and Subcellular locations from COMPARTMENTS for MLK4 Gene

Pathways & Interactions for MLK4 Gene

SuperPathways for MLK4 Gene

No Data Available

Gene Ontology (GO) - Biological Process for MLK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000186 activation of MAPKK activity IEA --
GO:0006468 protein phosphorylation ISS --
GO:0007257 activation of JUN kinase activity ISS --
GO:0046777 protein autophosphorylation ISS --
genes like me logo Genes that share ontologies with MLK4: view

No data available for Pathways by source and SIGNOR curated interactions for MLK4 Gene

Drugs & Compounds for MLK4 Gene

(1) Drugs for MLK4 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Magnesium Nutra 0
genes like me logo Genes that share compounds with MLK4: view

Transcripts for MLK4 Gene

Unigene Clusters for MLK4 Gene

Mixed lineage kinase 4:
Representative Sequences:

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for MLK4 Gene

No ASD Table

Relevant External Links for MLK4 Gene

GeneLoc Exon Structure for
MLK4

Expression for MLK4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

SOURCE GeneReport for Unigene cluster for MLK4 Gene:

Hs.547779
genes like me logo Genes that share expression patterns with MLK4: view

Primer Products

No data available for mRNA expression in normal human tissues , mRNA differential expression in normal tissues , Protein differential expression in normal tissues , Protein expression , Protein tissue co-expression partners and mRNA Expression by UniProt/SwissProt for MLK4 Gene

Orthologs for MLK4 Gene

This gene was present in the common ancestor of animals.

Orthologs for MLK4 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia LOC616014 34
  • 82.92 (n)
  • 81.37 (a)
-- 35
  • 76 (a)
OneToOne
dog
(Canis familiaris)
Mammalia KIAA1804 34
  • 83.32 (n)
  • 80.36 (a)
-- 35
  • 84 (a)
OneToOne
mouse
(Mus musculus)
Mammalia BC021891 34
  • 73.98 (n)
  • 71.33 (a)
BC021891 16
BC021891 35
  • 71 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia LOC469711 34
  • 99 (n)
  • 98.65 (a)
-- 35
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia RGD1306091 34
  • 73.75 (n)
  • 72.79 (a)
oppossum
(Monodelphis domestica)
Mammalia -- 35
  • 82 (a)
OneToOne
chicken
(Gallus gallus)
Aves LOC428586 34
  • 71.43 (n)
  • 69.88 (a)
-- 35
  • 66 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia -- 35
  • 64 (a)
OneToOne
zebrafish
(Danio rerio)
Actinopterygii si:ch211-120p12.3 34
  • 63.99 (n)
  • 64.21 (a)
si:ch211-120p12.3 35
  • 58 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta slpr 35
  • 30 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.8999 35
  • 34 (a)
OneToMany
Species where no ortholog for MLK4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MLK4 Gene

ENSEMBL:
Gene Tree for MLK4 (if available)
TreeFam:
Gene Tree for MLK4 (if available)

Paralogs for MLK4 Gene

(12) SIMAP similar genes for MLK4 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with MLK4: view

Variants for MLK4 Gene

Sequence variations from dbSNP and Humsavar for MLK4 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type
rs35465006 - 233,354,958(+) AAGAT(A/G)ACTGG reference, missense
rs35758282 - 233,375,929(+) GATGA(A/C)AGCAA reference, missense
rs34984140 - 233,376,030(+) AAATT(C/T)CATTC reference, missense
rs3795375 - 233,379,188(-) AAGGA(C/T)GGTGC reference, missense
rs3795374 - 233,379,229(-) TGAAG(A/C)TCTCT reference, missense

Structural Variations from Database of Genomic Variants (DGV) for MLK4 Gene

Variant ID Type Subtype PubMed ID
esv22442 CNV gain 19812545
esv2431778 CNV insertion 19546169
esv3305388 CNV mobile element insertion 20981092
esv3309615 CNV mobile element insertion 20981092
esv3325227 CNV insertion 20981092
esv3325872 CNV insertion 20981092
esv3354604 CNV insertion 20981092
nsv1069391 CNV deletion 25765185
nsv1112566 CNV deletion 24896259
nsv1118149 CNV deletion 24896259

Variation tolerance for MLK4 Gene

Residual Variation Intolerance Score: 87% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 11.70; 93.25% of all genes are more intolerant (likely to be disease-causing)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Relevant External Links for MLK4 Gene

Disorders for MLK4 Gene

No disorders were found for MLK4 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot , Genatlas and External Links for MLK4 Gene

Publications for MLK4 Gene

  1. MLK4 has negative effect on TLR4 signaling. (PMID: 21602844) Seit-Nebi A. … Han J. (Cell. Mol. Immunol. 2012) 3 4 65
  2. The DNA sequence and biological annotation of human chromosome 1. (PMID: 16710414) Gregory S.G. … Bentley D.R. (Nature 2006) 3 4 65
  3. Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PMID: 11347906) Nagase T. … Ohara O. (DNA Res. 2001) 3 4 65
  4. Serine/Threonine Kinase MLK4 Determines Mesenchymal Identity in Glioma Stem Cells in an NF-I_B-dependent Manner. (PMID: 26859459) Kim S.H. … Nakano I. (Cancer Cell 2016) 3 65
  5. SR protein kinases promote splicing of nonconsensus introns. (PMID: 26167880) Lipp J.J. … Guthrie C. (Nat. Struct. Mol. Biol. 2015) 3 65

Products for MLK4 Gene

Sources for MLK4 Gene

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