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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

MKI67IP Gene

protein-coding   GIFtS: 49
GCID: GC02M122484

MKI67 (FHA domain) interacting nucleolar phosphoprotein

  Search for MKI67IP
in our new
 Human Malady Compendium 
Biological research products
for MKI67IP
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
MKI67 (FHA Domain) Interacting Nucleolar Phosphoprotein1 2     Nucleolar Protein Interacting With The FHA Domain Of PKI-672 3
NIFK1 2 3 5     HNIFK1
Nopp341 2     NOPP343 5
Nucleolar Phosphoprotein Nopp342 3     MKI67 FHA Domain-Interacting Nucleolar Phosphoprotein2

External Ids:    HGNC: 178381   Entrez Gene: 843652   Ensembl: ENSG000001554387   OMIM: 6119705   UniProtKB: Q9BYG33   

Export aliases for MKI67IP gene to outside databases

Previous GC identifers: GC02M119689 GC02M120404 GC02M122391 GC02M122579 GC02M122200 GC02M114807


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for MKI67IP:
This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded
protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on
chromosomes 5, 10, 12, 15, and 19.(provided by RefSeq, Jan 2009)

Gene Wiki entry for MKI67IP


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000002.11  NC_018913.1  NT_022135.16  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the MKI67IP gene promoter:
         Max1   AhR   GR   Sox5   HNF-1A   Arnt   PPAR-alpha   HNF-1   GR-alpha   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMKI67IP promoter sequence
   Search SABiosciences Chromatin IP Primers for MKI67IP

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MKI67IP


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2q14.3   Ensembl cytogenetic band:  2q14.3   HGNC cytogenetic band: 2q14.3

MKI67IP Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MKI67IP gene location

GeneLoc information about chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02M122484:  view genomic region     (about GC identifiers)

Start:
122,484,521 bp from pter      End:
122,494,503 bp from pter
Size:
9,983 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: MK67I_HUMAN, Q9BYG3 (See protein sequence)
Recommended Name: MKI67 FHA domain-interacting nucleolar phosphoprotein  
Size: 293 amino acids; 34222 Da
Subunit: Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis
Subcellular location: Nucleus, nucleolus. Chromosome. Note=Localizes to mitotic chromosomes in conjunction with MKI67
1 PDB 3D structure from and Proteopedia for MKI67IP:
2AFF (3D)    
Secondary accessions: A8K788 Q8TB66 Q96ED4

Explore the universe of human proteins at neXtProt for MKI67IP: NX_Q9BYG3

Post-translational modifications:

  • Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is
  • phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated.
    Phosphorylation enhances MKI67 binding1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9BYG3

  • MKI67IP Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_115766.3  
    ENSEMBL proteins: 
     ENSP00000285814   ENSP00000386449   ENSP00000390174   ENSP00000406227   ENSP00000398116  

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    Uscn Proteins for MKI67IP

    Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000794condensed nuclear chromosome IDA11342549
    GO:0005654nucleoplasm IDA11342549
    GO:0005730nucleolus IDA11342549
    GO:0005737cytoplasm IDA11342549


    MKI67IP for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    MKI67IP for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR012677 Nucleotide-bd_a/b_plait
     IPR000504 RRM_dom
     IPR021043 hNIFK_FHA_Ki67_binding

    Graphical View of Domain Structure for InterPro Entry Q9BYG3

    ProtoNet protein and cluster: Q9BYG3

    1 Blocks protein family: IPB000504 RNA-binding region RNP-1 (RNA recognition motif)

    UniProtKB/Swiss-Prot: MK67I_HUMAN, Q9BYG3
    Similarity: Contains 1 RRM (RNA recognition motif) domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    miRNA
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    Inhib. RNA
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    Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166nucleotide binding IEA--
    GO:0003676nucleic acid binding ----
    GO:0003723RNA binding IDA11342549
    GO:0005515protein binding IPI11342549


    MKI67IP for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for MKI67IP:
     Synthetic lethal with Ras 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for MKI67IP

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/97 Interacting proteins for MKI67IP (Q9BYG32, 3 ENSP000002858144) via UniProtKB, MINT, STRING, and/or I2D (see all 97)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    MKI67P460132, 3, ENSP000003576434MINT-50550 I2D: score=2 STRING: ENSP00000357643
    GABARAPO951663I2D: score=1 
    PPP1CAENSP000003260314STRING: ENSP00000326031
    CBX8ENSP000002693854STRING: ENSP00000269385
    NSA2ENSP000002968024STRING: ENSP00000296802
    About this table

    Gene Ontology (GO): 4 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006461protein complex assembly NAS11342549
    GO:0009303rRNA transcription NAS11342549
    GO:0010923negative regulation of phosphatase activity IDA19389623
    GO:0016072rRNA metabolic process NAS11342549


    MKI67IP for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for MKI67IP
    Search CenterWatch for drugs/clinical trials and news about MKI67IP / MK67I 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for MKI67IP gene: 
    NM_032390.4  

    Unigene Cluster for MKI67IP:

    MKI67 (FHA domain) interacting nucleolar phosphoprotein
    Hs.367842  [show with all ESTs]
    Unigene Representative Sequence: NM_032390
    8 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000285814(uc002tnk.3) ENST00000409201 ENST00000423105 ENST00000481978
    ENST00000498570 ENST00000447132 ENST00000451734 ENST00000477693(uc010fls.3)


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    Inhib. RNA
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    Additional cDNA sequence: 

    AB044971.1 AK027097.1 AK291903.1 AK299814.1 AK311033.1 BC012457.1 BC022990.1 BC024238.2 

    10 DOTS entries:

    DT.416793  DT.97845050  DT.92450601  DT.97778505  DT.121000388  DT.97804207  DT.100028979  DT.100733042 
    DT.92450605  DT.99995751 

    24/350 AceView cDNA sequences (see all 350):

    BF589119 BG058573 AI497673 AW960765 BM799591 BP336789 AA765363 BE042711 
    BC012457 CR620927 BF063907 AA513403 AA456437 BF149128 BC022990 BU190916 
    BU198391 AA639548 BM505270 AI217616 BU620726 AI668969 BM974502 AA281395 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for MKI67IP    About this scheme

    ExUns: 1 ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7a · 7b · 7c
    SP1:        -                                                         
    SP2:                                                                  
    SP3:                                      -                           


    ECgene alternative splicing isoforms for MKI67IP

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    MKI67IP expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: AATTAATAAC

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See MKI67IP Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for MKI67IP

    SOURCE GeneReport for Unigene cluster: Hs.367842
        SABiosciences Custom PCR Arrays for MKI67IP
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    In Situ
    Assay Products:
     

     
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for MKI67IP gene from 9/30 species (see all 30)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Mki67ip1 , 5 Mki67 (FHA domain) interacting nucleolar phosphoprotein1, 5 69.64(n)1
    62.2(a)1
      1 (52.14 cM)5
    679491  NM_026472.41  NP_080748.31 
     1183218395 
    chicken
    (Gallus gallus)
    Aves MKI67IP1 MKI67 (FHA domain) interacting nucleolar phosphoprotein 59.19(n)
    54.75(a)
      424239  XM_422088.2  XP_422088.1 
    lizard
    (Anolis carolinensis)
    Reptilia MKI67IP6
    --
    53(a)
    1 ↔ 1
    3(2135472-2142996)
    African clawed frog
    (Xenopus laevis)
    Amphibia LOC3986452 hypothetical protein LOC398645 76.41(n)    BC054288.1 
    zebrafish
    (Danio rerio)
    Actinopterygii mki67ipl2 mki67 (FHA domain) interacting nucleolar phosphoprotein more 79.12(n)   317644  AF506200.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG69371 CG6937 43.51(n)
    34.94(a)
      42662  NM_142809.3  NP_651066.2 
    worm
    (Caenorhabditis elegans)
    Secernentea T04A8.66
    Protein T04A8.6
    20(a)
    1 ↔ 1
    III(4692057-4693264)
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AT5G046001 RNA recognition motif-containing protein 47.35(n)
    37.5(a)
      830337  NM_120542.4  NP_196080.1 
    rice
    (Oryza sativa)
    Liliopsida Os08g04122001 hypothetical protein 47.71(n)
    38.75(a)
      4345558  NM_001068328.1  NP_001061793.1 


    ENSEMBL Gene Tree for MKI67IP (if available)
    TreeFam Gene Tree for MKI67IP (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for MKI67IP gene

    MKI67IP for paralogs           About GeneDecksing


    5/12 Pseudogenes.org Pseudogenes for MKI67IP (see all 12)
    PGOHUM00000238710 PGOHUM00000239307 PGOHUM00000246855 PGOHUM00000246910 PGOHUM00000246911


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/330 NCBI SNPs in MKI67IP are shown (see all 330    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 2 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs353969141,2
    C--122484095(+) TTTTTT/-GAGAC 2 -- ds5001 int11Minor allele frequency- -:0.00NA 2
    rs587261641,2
    C,F,--122484315(+) TTGATT/ATCTTG 2 -- ds5001 int12Minor allele frequency- A:0.50NA CSA 4
    rs1162755281,2
    F,--122484374(+) AGCAAG/CCTACC 2 -- int1 ds50011Minor allele frequency- C:0.06WA 118
    rs1154110291,2
    F,--122484454(+) AACTCC/TTAGCC 2 -- ds5001 int11Minor allele frequency- T:0.03WA 118
    rs1850843281,2
    --122484507(+) ACAGGA/CACAAG 2 -- ds5001 int10--------
    rs1899384901,2
    --122484511(+) GCACAA/GGCTAC 2 -- int1 ds50010--------
    rs1477246291,2
    --122484528(+) TGATGA/GCAATG 2 -- ut31 int10--------
    rs1824852691,2
    --122484586(+) TTTTTC/TCCCAA 2 -- ut31 int10--------
    rs170067651,2
    C,F,H,--122484622(+) AAATAC/AATTGT 2 -- int1 ut3122Minor allele frequency- A:0.19NA NS EA WA CSA 2480
    rs770358571,2
    C,F,--122484625(+) TACATT/CGTAGA 2 -- ut31 int11Minor allele frequency- C:0.02WA 118

    HapMap Linkage Disequilibrium report for MKI67IP (122484521 - 122494503 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for MKI67IP: --

    SABiosciences Cancer Mutation PCR Assays
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    DNA2.0 Custom Variant and Variant Library Synthesis for MKI67IP

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    MKI67IP for disorders           About MalaCards

    MKI67IP for disorders           About GeneDecksing

    OMIM gene information: 611970    OMIM disorders: --


    Export disorders for MKI67IP gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for MKI67IP gene, integrated from 9 sources (see all 27):
    (articles sorted by number of sources associating them with MKI67IP)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis. (PubMed id 11342549)1, 2, 3, 9 Takagi M.... Yoneda Y. (2001)
    2. Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)1, 2 Hillier L.W....Wilson R.K. (2005)
    3. Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67. (PubMed id 16244663)1, 2 Byeon I.-J.... Tsai M.-D. (2005)
    4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    5. Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions. (PubMed id 14659764)1, 2 Li H.... Tsai M.-D. (2004)
    6. A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
    7. Functional proteomics establishes the interaction of S IRT7 with chromatin remodeling complexes and expands its role in regulation of R NA polymerase I transcription. (PubMed id 22586326)1 Tsai Y.C....Cristea I.M. (2012)
    8. A catalogue of putative HIV-1 protease host cell subst rates. (PubMed id 22944692)1 Impens F....Gevaert K. (2012)
    9. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
    10. Direct observations of shifts in the I^-sheet register of a protein-peptide complex using explicit solvent simulations. (PubMed id 21539773)1 Panteva M.T....Chong L.T. (2011)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 84365 HGNC: 17838 AceView: MKI67IP Ensembl:ENSG00000155438 euGenes: HUgn84365
    ECgene: MKI67IP H-InvDB: MKI67IP

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for MKI67IP Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for MKI67IP gene:
    Search GeneIP for patents involving MKI67IP

    GeneCards and IP:
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