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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

MIF4GD Gene

protein-coding   GIFtS: 43
GCID: GC17M073262

MIF4G domain containing


(Previous symbol: MIFD)
 Explore 1 disease affiliated with
MIF4GD via our new
 Human Malady Compendium 
Biological research products
for MIF4GD
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
MIF4G Domain Containing1 2     MGC450271
SLIP12 3 5     MIF4G Domain-Containing Protein2
AD0231 2     SLBP (Stem Loop Binding Protein)-Interacting Protein 12
MIFD1 2     HSLIP11
SLBP-Interacting Protein 12 3     

External Ids:    HGNC: 240301   Entrez Gene: 574092   Ensembl: ENSG000001254577   OMIM: 6120725   UniProtKB: A9UHW63   

Export aliases for MIF4GD gene to outside databases

Previous GC identifers: GC17M070774 GC17M068672


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for MIF4GD:
This gene encodes a protein which interacts with the N-terminus of the stem-loop binding protein (SLBP) and the 3' end
of histone mRNA. This interaction facilitates the activation of histone mRNA translation. Alternative splicing results
in multiple transcript variants encoding different isoforms. (provided by RefSeq, Jun 2011)

UniProtKB/Swiss-Prot: MI4GD_HUMAN, A9UHW6
Function: Functions in replication-dependent translation of histone mRNAs which differ from other eukaryotic mRNAs in
that they do not end with a poly-A tail but a stem-loop. May participate in circularizing those mRNAs specifically
enhancing their translation




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000017.10  NC_018928.1  NT_010783.15  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the MIF4GD gene promoter:
         ER-alpha   Pax-2   Lmo2   Pax-2a   AP-4   E4BP4   E47   c-Ets-1   c-Myb   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMIF4GD promoter sequence
   Search SABiosciences Chromatin IP Primers for MIF4GD

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MIF4GD


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 17q25.1   Ensembl cytogenetic band:  17q25.1   HGNC cytogenetic band: 17q25.1

MIF4GD Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MIF4GD gene location

GeneLoc information about chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17M073262:  view genomic region     (about GC identifiers)

Start:
73,262,309 bp from pter      End:
73,267,308 bp from pter
Size:
5,000 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: MI4GD_HUMAN, A9UHW6 (See protein sequence)
Recommended Name: MIF4G domain-containing protein  
Size: 222 amino acids; 25423 Da
Subunit: Interacts with EIF4G1, EIF4G2 and SLBP; probably tethered by SLBP to the 3'-end of mRNAs ending with the
histone stem-loop, it also interacts with EIF4G1 which is bound to their 5'-end
Subcellular location: Cytoplasm. Nucleus
Miscellaneous: Depletion of MIF4GD results in cell death and reduced histone mRNA translation
Sequence caution: Sequence=AAG09724.1; Type=Frameshift; Positions=Several;
Secondary accessions: Q8N4Q5 Q9HBL5
Alternative splicing: 2 isoforms:  A9UHW6-1   A9UHW6-2   

Explore the universe of human proteins at neXtProt for MIF4GD: NX_A9UHW6

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_A9UHW6

  • MIF4GD Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (4 alternative transcripts): 
    NP_001229427.1  NP_001229429.1  NP_001229430.1  NP_065730.2  

    ENSEMBL proteins: 
     ENSP00000321625   ENSP00000245551   ENSP00000462459   ENSP00000463334   ENSP00000462412  
     ENSP00000463579   ENSP00000463629   ENSP00000464448   ENSP00000463264   ENSP00000462655  
     ENSP00000463493  

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    Uscn Proteins for MIF4GD

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IEA--
    GO:0005737cytoplasm IEA--


    MIF4GD for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    MIF4GD for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR003890 MIF4G-like_typ-3
     IPR016021 MIF4-like_typ_1/2/3
     IPR016024 ARM-type_fold

    Graphical View of Domain Structure for InterPro Entry A9UHW6

    ProtoNet protein and cluster: A9UHW6

    UniProtKB/Swiss-Prot: MI4GD_HUMAN, A9UHW6
    Similarity: Belongs to the MIF4GD family
    Similarity: Contains 1 MIF4G domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: MI4GD_HUMAN, A9UHW6
    Function: Functions in replication-dependent translation of histone mRNAs which differ from other eukaryotic mRNAs in
    that they do not end with a poly-A tail but a stem-loop. May participate in circularizing those mRNAs specifically
    enhancing their translation

    miRNA
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    Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677DNA binding IEA--
    GO:0003723RNA binding IEA--
    GO:0005515protein binding IPI--
    GO:0008022protein C-terminus binding IPI17577209


    MIF4GD for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for MIF4GD

    STRING Interaction Network Preview (showing 5 interactants - click image to see 8)

    5/11 Interacting proteins for MIF4GD (A9UHW61, 3 ENSP000002455514) via UniProtKB, MINT, STRING, and/or I2D (see all 11)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    EXOSC6Q5RKV63, ENSP000003985974I2D: score=2 STRING: ENSP00000398597
    SVILO954253, ENSP000003481284I2D: score=1 STRING: ENSP00000348128
    UBQLN4Q9NRR53, ENSP000003572924I2D: score=3 STRING: ENSP00000357292
    DDX19AQ9NUU73, ENSP000003061174I2D: score=5 STRING: ENSP00000306117
    HGSO149643, ENSP000003312014I2D: score=5 STRING: ENSP00000331201
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006417regulation of translation IEA--
    GO:0016070RNA metabolic process IEA--


    MIF4GD for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for MIF4GD
    Search CenterWatch for drugs/clinical trials and news about MIF4GD / MI4GD 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
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    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for MIF4GD gene (4 alternative transcripts): 
    NM_001242498.1  NM_001242500.1  NM_001242501.1  NM_020679.3  

    Unigene Cluster for MIF4GD:

    MIF4G domain containing
    Hs.325631  [show with all ESTs]
    Unigene Representative Sequence: NM_001242498
    13 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000325102(uc021ucx.1 uc002jnr.3) ENST00000245551 ENST00000579297(uc002jnp.3 uc002jnq.3)
    ENST00000577542 ENST00000580571 ENST00000579119 ENST00000578305 ENST00000580717
    ENST00000579612 ENST00000577240 ENST00000579194 ENST00000581777 ENST00000580099


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    Additional cDNA sequence: 

    AK300711.1 BC033759.1 

    18 DOTS entries:

    DT.456328  DT.100021262  DT.95271450  DT.40132806  DT.100699845  DT.100775144  DT.100796367  DT.121005217 
    DT.91766617  DT.121005108  DT.121005238  DT.91766616  DT.91831401  DT.95271449  DT.99975963  DT.100796358 
    DT.121005112  DT.91766610 

    24/144 AceView cDNA sequences (see all 144):

    CA488406 BX332441 T24480 CD244825 BQ063767 BU190758 CR597523 BM983086 
    CD364733 BM719785 CA418646 AA743832 BI030881 BF726619 CR605810 AA592948 
    BU739657 CR595273 BQ330591 CR623253 BM694825 BM671199 BM710043 AA321029 

    GeneLoc Exon Structure

    5/11 Alternative Splicing Database (ASD) splice patterns (SP) for MIF4GD (see all 11)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2 ^ 3a · 3b · 3c · 3d · 3e ^ 4a · 4b ^ 5a · 5b ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b · 8c · 8d · 8e
    SP1:                    -     -                                               -                       -                                 
    SP2:                          -                                               -                       -                                 
    SP3:                    -     -                             -     -           -                       -                                 
    SP4:                                                        -                 -                       -                                 
    SP5:                    -     -                             -     -           -     -     -           -                                 


    ECgene alternative splicing isoforms for MIF4GD

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    MIF4GD expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: CTACACCAGT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See MIF4GD Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for MIF4GD

    SOURCE GeneReport for Unigene cluster: Hs.325631
        SABiosciences Custom PCR Arrays for MIF4GD
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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for MIF4GD gene from 6/16 species (see all 16)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves LOC1008588481 MIF4G domain-containing protein-like 78.68(n)
    83.78(a)
      100858848  XM_003642362.1  XP_003642410.1 
    lizard
    (Anolis carolinensis)
    Reptilia MIF4GD6
    --
    72(a)
    1 ↔ 1
    2(114647203-114652342)
    African clawed frog
    (Xenopus laevis)
    Amphibia MGC535132 hypothetical protein MGC53513 79.55(n)    BC048773.1 
    zebrafish
    (Danio rerio)
    Actinopterygii zgc641522 hypothetical protein MGC64152 72.08(n)   393355  BC053283.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG131246
    --
    10(a)
    1 → many
    2L(9903379-9908633)
    worm
    (Caenorhabditis elegans)
    Secernentea F44A2.56
    Protein F44A2.5, isoform b
    12(a)
    1 → many
    V(9297608-9302254)


    ENSEMBL Gene Tree for MIF4GD (if available)
    TreeFam Gene Tree for MIF4GD (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for MIF4GD gene
    CTIF2  

    MIF4GD for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/119 NCBI SNPs in MIF4GD are shown (see all 119    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 17 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs2020196211,2
    --73262984(+) CCATGC/TGCTGC 8 H R mis10--------
    rs2012347901,2
    --73262989(+) CGCTGC/TCCATT 8 G syn10--------
    rs617393241,2
    C,F,--73263012(+) CTGCTC/TCCCAA 8 K E mis18Minor allele frequency- T:0.06NS WA CSA EA NA EU 5916
    rs2004013921,2
    --73263018(+) CCCAAC/TCCGGT 8 I V mis10--------
    rs802126671,2
    C,--73263150(+) ACTCAT/CTCCCT 4 -- int13Minor allele frequency- C:0.05CSA WA NA 240
    rs1420709081,2
    --73263205(+) GAAAGA/GAATGA 4 -- int10--------
    rs1463291021,2
    --73263216(+) GTTCCA/GGGACT 4 -- int10--------
    rs112677751,2
    C,--73263266(+) GTCTC-/CAAAC 
    CACTTCTT
    CAAAC
    4 -- int10--------
    rs558155001,2
    C--73263281(+) CTTCAAACCACT 
    TCTTCA
    /-
    TCCCA
    4 -- int11Minor allele frequency- -:0.00NA 2
    rs47891621,2
    C,F,A,H,--73263282(+) CTTCAT/ACCCAC 4 -- int11Minor allele frequency- A:0.28NA 120

    HapMap Linkage Disequilibrium report for MIF4GD (73262309 - 73267308 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for MIF4GD: --

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing MIF4GD
    DNA2.0 Custom Variant and Variant Library Synthesis for MIF4GD

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    MIF4GD for disorders           About GeneDecksing

    OMIM gene information: 612072    OMIM disorders: --

    1 disease for MIF4GD:    About MalaCards
    ataxia


    Export disorders for MIF4GD gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for MIF4GD gene, integrated from 9 sources (see all 12):
    (articles sorted by number of sources associating them with MIF4GD)
        Utopia: connect your pdf to the dynamic
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    1. SLIP1, a factor required for activation of histone mRNA translation by the stem-loop binding protein. (PubMed id 18025107)1, 2 Cakmakci N.G....Marzluff W.F. (2008)
    2. Assembly of the SLIP1-SLBP complex on histone mRNA req uires heterodimerization and sequential binding of SLBP followed by SLIP1. (PubMed id 23286197)1 Bansal N....Thapar R. (2013)
    3. A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
    4. INT6 interacts with MIF4GD/SLIP1 and is necessary for efficient histone mRNA translation. (PubMed id 22532700)1 Neusiedler J....Jalinot P. (2012)
    5. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
    6. Novel interactors and a role for supervillin in early cytokinesis. (PubMed id 20309963)1 Smith T.C....Luna E.J. (2010)
    7. The interactome of the histone gene regulatory factor HiNF-P suggests novel cell cycle related roles in transcriptional control and RNA processing. (PubMed id 17577209)1 Miele A....Stein J.L. (2007)
    8. A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. (PubMed id 16713569)1 Lim J.... Zoghbi H.Y. (2006)
    9. Towards a proteome-scale map of the human protein-protein interaction network. (PubMed id 16189514)1 Rual J.F....Vidal M. (2005)
    10. A protein interaction framework for human mRNA degradation. (PubMed id 15231747)1 Lehner B. and Sanderson C.M. (2004)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 57409 HGNC: 24030 AceView: AD023 Ensembl:ENSG00000125457 euGenes: HUgn57409
    ECgene: MIF4GD H-InvDB: MIF4GD

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for MIF4GD Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
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    IP news from LifeMap Sciences, Inc.)
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    Patent Information for MIF4GD gene:
    Search GeneIP for patents involving MIF4GD

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